pGLO Transformation Lab by Erica Olsen https://padlet.com/eolsen2/31o187dqz0gd en-us 2016-03-08 04:15:07 UTC 2025-05-11 19:12:17 UTC hello@padlet.com The Guiding Question : By Sami Guiterrez eolsen2 https://padlet.com/eolsen2/31o187dqz0gd/wish/99564592 2016-03-08 04:19:51 UTC https://padlet.com/eolsen2/31o187dqz0gd/wish/99564592 Our Claim : By Sami Guiterrez eolsen2 https://padlet.com/eolsen2/31o187dqz0gd/wish/99564601 2016-03-08 04:20:08 UTC https://padlet.com/eolsen2/31o187dqz0gd/wish/99564601 Our Evidence : by Erica Olsen eolsen2 https://padlet.com/eolsen2/31o187dqz0gd/wish/99564619 The evidence we collected was based off of the observations we saw from the colonies grown on the LB/amp/ara plate, which totalled to one colony. From there we could determine the amount of pGLO DNA spread, which can be calculated by multiplying the total amount of DNA used by the fraction of DNA used, in this experiment it came out to 0.2. Using these two numbers, the transformation efficency can be caluclated, as it is the total number of colonies growing on the agar plate divided by the amount of DNA spread on the agar plate, which totalled to 5 (ug). ]]> 2016-03-08 04:20:22 UTC https://padlet.com/eolsen2/31o187dqz0gd/wish/99564619 Our Justification of the Evidence : by Enun Enun eolsen2 https://padlet.com/eolsen2/31o187dqz0gd/wish/99564627 2016-03-08 04:20:32 UTC https://padlet.com/eolsen2/31o187dqz0gd/wish/99564627 Transformation pGLO Lab eolsen2 https://padlet.com/eolsen2/31o187dqz0gd/wish/99564750 by: Sami Guiterrez, Enun Enun, Erica Olsen]]> 2016-03-08 04:22:27 UTC https://padlet.com/eolsen2/31o187dqz0gd/wish/99564750 sgutierrez7 https://padlet.com/eolsen2/31o187dqz0gd/wish/99565370 Can the insertion of the GFP gene from bioluminescent jellyfish into e. coli change/transform e. coli into a bioluminescent form/phenotype by expression of the GFP gene into the green fluorescent protein that glows green? ]]> 2016-03-08 04:35:01 UTC https://padlet.com/eolsen2/31o187dqz0gd/wish/99565370 sgutierrez7 https://padlet.com/eolsen2/31o187dqz0gd/wish/99566640 Yes, the insertion of the GFP gene from bioluminescent jellyfish into e. coli can change/transform e. coli into a bioluminescent form/phenotype by expression of the GFP gene into the green fluorescent protein that glows green.]]> 2016-03-08 04:59:32 UTC https://padlet.com/eolsen2/31o187dqz0gd/wish/99566640 sgutierrez7 https://padlet.com/eolsen2/31o187dqz0gd/wish/99576853 2016-03-08 07:40:46 UTC https://padlet.com/eolsen2/31o187dqz0gd/wish/99576853