https://padlet.com/biologydept/3170hpkg7utv Please use this platform to ask questions about the practical. Your questions and our responses are available to everyone. We will check in periodically to answer your queries. en-us 2020-03-23 11:00:24 UTC 2020-05-05 14:43:18 UTC hello@padlet.com biologydept https://padlet.com/biologydept/3170hpkg7utv/wish/470723612 What information should I be able to get from the seedling pictures?]]> 2020-03-23 11:02:19 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/470723612 biologydept https://padlet.com/biologydept/3170hpkg7utv/wish/470724520 Thank you for your question you should be able to see information on colour, size, and whether skotomorphogenesis or photomorphogenesis has taken place. You can see this information for all the different seedlings grown in different light conditions.

]]> 2020-03-23 11:02:47 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/470724520 https://padlet.com/biologydept/3170hpkg7utv/wish/488520650 What is meant by 'normal margins'? And should the report be structured in just two parts: Section 1 and Section 2? Or should it be structured traditionally with an Introduction, Results, Discussion etc.?]]> 2020-04-02 10:05:58 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/488520650 biologydept https://padlet.com/biologydept/3170hpkg7utv/wish/488662897 In word, if you click the 'layout' tab, there is an option to set margin size as normal, narrow, moderate, wide or custom. Please click on normal. No introduction necessary, just 2 sections please- Mutant Identifications and Discussion.]]> 2020-04-02 11:28:01 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/488662897 https://padlet.com/biologydept/3170hpkg7utv/wish/489036163 Do we need to use the template in the assessment section of Green Planet on Blackboard? (and therefore use the cover page and line numbers?)]]> 2020-04-02 14:13:21 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/489036163 https://padlet.com/biologydept/3170hpkg7utv/wish/494272097 How should we figure out what genotype D is? I can't seem to find any mutants/combinations on the powerpoint that fit. Any hints would be appreciated]]> 2020-04-06 08:41:09 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/494272097 https://padlet.com/biologydept/3170hpkg7utv/wish/494637628 When I am trying to explain my reasoning in the mutant identification section, I am worried that I am including information that would be traditionally written in the discussion section. Will this lose me marks, or does it not matter where the information goes in this report, as long as it is mentioned? ]]> 2020-04-06 12:24:10 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/494637628 biologydept https://padlet.com/biologydept/3170hpkg7utv/wish/494814739 For Genotype D, you shoud try and think about a link between photoreceptors and  chlorophyll. This should led you there. I'm afraid I can't give too much information or it would be too easy.

]]> 2020-04-06 13:45:08 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/494814739 biologydept https://padlet.com/biologydept/3170hpkg7utv/wish/494818786 As a suggestion, you don't need to include references in the mutant identification section. Just explain the phenotypes. Your discussion should show that you have read some papers about these mutants. I hope this helps.]]> 2020-04-06 13:46:49 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/494818786 https://padlet.com/biologydept/3170hpkg7utv/wish/502202811 I feel like I am repeating myself a lot when describing the phenotypes of each A - G seedling in the 5 light conditions. Is this expected, or should I be condensing the paragraphs to focus mainly on the mutant phenotypes? ]]> 2020-04-10 15:53:53 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/502202811 https://padlet.com/biologydept/3170hpkg7utv/wish/503576942 2020-04-12 15:19:15 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/503576942 https://padlet.com/biologydept/3170hpkg7utv/wish/504299582 I'm very confused about the phenotypes of seedlings A and B when grown in white light. Are you able to give some insight in to the reasons for the medium hypocotyl length for both of these genotypes? My current thoughts are ....
1. It has something to do with COP-mediated signalling (if either Pfr or CRY1 are not present then is the degradation of hy5 changed in some way?)
2. Is it to do with leaky chromophore mutations (hy1 and hy2 mutants as seen in the narrated ppt.)
3. OR am i over complicating it and the reason for this phenotype is that white light is a mixture of red and blue light and when only one photoreceptor is working you get some sort of mid-point between photomorphogenesis and skotomorphogenesis. ]]> 2020-04-13 09:59:51 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/504299582 https://padlet.com/biologydept/3170hpkg7utv/wish/505020916 what would be the correct nomenclature for a mutant lacking chromophores in all phytochromes (such as the case in the hy1 phenotype)? + is it possible for a mutant to lack a chromophore in just one phytochrome group - e.g. PHYA, seeing as all phytochrome use the same chromophores]]> 2020-04-13 18:07:59 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/505020916 https://padlet.com/biologydept/3170hpkg7utv/wish/505934929 Are references included in page count?]]> 2020-04-14 09:08:46 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/505934929 https://padlet.com/biologydept/3170hpkg7utv/wish/505981446 in the instructions it said that all the mutation are discussed on the powerpoint - is D definitely on there or do we need to do extra research?]]> 2020-04-14 09:36:03 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/505981446 https://padlet.com/biologydept/3170hpkg7utv/wish/508876141 Yes, references are include in the page count

]]> 2020-04-15 16:12:07 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/508876141 https://padlet.com/biologydept/3170hpkg7utv/wish/508877815 All the mutants were discussed in my lectures. Extra research should provide extra details.]]> 2020-04-15 16:12:49 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/508877815 https://padlet.com/biologydept/3170hpkg7utv/wish/512876253 Are they all loss of function mutants, or can they also be over expression mutants? ]]> 2020-04-17 13:43:36 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/512876253 https://padlet.com/biologydept/3170hpkg7utv/wish/516679896 2020-04-20 07:37:16 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/516679896 https://padlet.com/biologydept/3170hpkg7utv/wish/516831819 There are no triple mutants. All are single or double. 

]]> 2020-04-20 08:41:51 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/516831819 https://padlet.com/biologydept/3170hpkg7utv/wish/517263031 On blackboard it states that the only thing included in the 3 page count is the mutant justification and discussion and then we also need a cover page and bibliography which is not included in the page count, however this contradicts information on this padlet board so will we get penalised for following either one?]]> 2020-04-20 11:57:39 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/517263031 https://padlet.com/biologydept/3170hpkg7utv/wish/518411064 Is there a difference between hy1 and hy2 mutants? is there going to be a difference if both of them are present at the same time?

]]> 2020-04-20 18:44:22 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/518411064 https://padlet.com/biologydept/3170hpkg7utv/wish/518427758 Would it be possible to have another hint for genotype D? I can't get my head around it at all

]]> 2020-04-20 18:51:30 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/518427758 https://padlet.com/biologydept/3170hpkg7utv/wish/520080582 The page limit includes everything- mutant justification, discussion and bibliography. No cover page needed. I will email out to everyone to clarify this.

]]> 2020-04-21 12:29:51 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/520080582 https://padlet.com/biologydept/3170hpkg7utv/wish/520084457 2020-04-21 12:31:42 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/520084457 https://padlet.com/biologydept/3170hpkg7utv/wish/520089876 First have a think about which photoreceptor responses are missing and then think about how these photoreceptors are made.]]> 2020-04-21 12:34:09 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/520089876 jm18933 https://padlet.com/biologydept/3170hpkg7utv/wish/521494875 I am a little confused what should go in the discussion, can you offer any guidance?

]]> 2020-04-21 21:58:21 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/521494875 https://padlet.com/biologydept/3170hpkg7utv/wish/522746556 2020-04-22 11:43:27 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/522746556 https://padlet.com/biologydept/3170hpkg7utv/wish/522845506 The results should simply describe the phenotypes of your mutants and justify the genotype identification. The discussion is a chance to show some background reading about the mutants.

]]> 2020-04-22 12:33:41 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/522845506 https://padlet.com/biologydept/3170hpkg7utv/wish/522847809 I would predict about a page. Each genotype requires a short paragraph of a few lines. Some require more justification than others. The page limit is a maximum. Quality is more important than quantity!

]]> 2020-04-22 12:34:45 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/522847809 https://padlet.com/biologydept/3170hpkg7utv/wish/523925393 it was mentioned that cry1 is also known as hy4 and hy3 as phyB. Would it be wrong to refer to a phyB mutant as hy3 and vice versa?


]]> 2020-04-22 18:50:34 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/523925393 https://padlet.com/biologydept/3170hpkg7utv/wish/525129621 As long as you specify that cry1=hy4 and phyB=hy3 then this is fine. 

]]> 2020-04-23 08:41:05 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/525129621 https://padlet.com/biologydept/3170hpkg7utv/wish/525252274 You mentioned that white light is composed of both red and blue light. But does this mean that the amount/quality of red light in white light is lower than the pure red light?

]]> 2020-04-23 09:32:11 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/525252274 https://padlet.com/biologydept/3170hpkg7utv/wish/526233930 is it okay if we describe what a WT phenotype looks like in each light condition once  and then simply refer to it as WT? or do we have to mention that eg in red light the plant behaves like WT because it has short hypocotyl, open cotyledons and green colour
every time we describe the phenotype of each plant

]]> 2020-04-23 16:10:07 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/526233930 https://padlet.com/biologydept/3170hpkg7utv/wish/528390854 The white light used here was 50% red and 50% blue. The amounts of red and blue light were equal to the amounts used in pure red and pure blue experiments (high irradiance). There is no UV.]]> 2020-04-24 13:50:20 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/528390854 https://padlet.com/biologydept/3170hpkg7utv/wish/528397444 If you accurately describe your WT phenotype in each light condition once, then it is fine to refer to 'a WT phenotype'.

]]> 2020-04-24 13:52:52 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/528397444 https://padlet.com/biologydept/3170hpkg7utv/wish/529942013 Just wanted to make sure page count, margin size and font size are the only structure requirements. Do we need to adhere to the normal structure requirements as well like 1.5 line spacing?]]> 2020-04-25 12:26:02 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/529942013 https://padlet.com/biologydept/3170hpkg7utv/wish/531401891 is the skotomorphogenesis phenotype different 
from the shade avoidance phenotype? 


]]> 2020-04-26 16:08:13 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/531401891 https://padlet.com/biologydept/3170hpkg7utv/wish/531441381 Can i write in the present tense "genotype C has a short cotyledon" or does it need to be past tense]]> 2020-04-26 16:31:28 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/531441381 https://padlet.com/biologydept/3170hpkg7utv/wish/531664118 Yes please- 1.5 line spacing

]]> 2020-04-26 18:52:49 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/531664118 https://padlet.com/biologydept/3170hpkg7utv/wish/531664993 These responses are very similar and both are characterised by stem elongation but have differences.]]> 2020-04-26 18:53:26 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/531664993 https://padlet.com/biologydept/3170hpkg7utv/wish/531675956 Either is fine as long as we understand what you are saying!

]]> 2020-04-26 19:00:44 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/531675956 https://padlet.com/biologydept/3170hpkg7utv/wish/534038516 Is the reduced chlorophyll in phenotype D only present in white (red & blue) light? or is it ]]> 2020-04-27 16:23:35 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/534038516 https://padlet.com/biologydept/3170hpkg7utv/wish/534327871 Yes, the reduced chlorophyll phenotype of genotype D is only observed in white light.

]]> 2020-04-27 18:03:57 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/534327871 https://padlet.com/biologydept/3170hpkg7utv/wish/534395301 is genotype D in white light supposed to be pale green OR yellow?

]]> 2020-04-27 18:29:42 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/534395301 https://padlet.com/biologydept/3170hpkg7utv/wish/534794403 It is very pale green but can sometimes appear yellow. What is important is that it has reduced chlorophyll synthesis.

]]> 2020-04-27 21:51:28 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/534794403 https://padlet.com/biologydept/3170hpkg7utv/wish/535784332 In the results we describe the phenotype so is the discussion for extra reading on the mechanisms behind the phenotypes, including the pathways and synthesis of the photoreceptors and genetic studies that led to these conclusions etc.?

]]> 2020-04-28 09:25:55 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/535784332 https://padlet.com/biologydept/3170hpkg7utv/wish/536139675 Spot on!!

]]> 2020-04-28 12:10:32 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/536139675 https://padlet.com/biologydept/3170hpkg7utv/wish/537276219 should phototropism be taken into account?

]]> 2020-04-28 18:36:46 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/537276219 https://padlet.com/biologydept/3170hpkg7utv/wish/538643808 For clarification can white light be considered to not have a higher irradiance  than solely blue light or solely red light? ]]> 2020-04-29 09:46:50 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/538643808 https://padlet.com/biologydept/3170hpkg7utv/wish/538935159 No. No phototropism phenotypes were shown for these mutants no nothing can be deduced about this response. 

]]> 2020-04-29 12:14:10 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/538935159 https://padlet.com/biologydept/3170hpkg7utv/wish/538936911 White light has the same amount of red and blue light as single treatments. The irradiance is therefore double. 

]]> 2020-04-29 12:15:04 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/538936911 https://padlet.com/biologydept/3170hpkg7utv/wish/540436442 When plants are grown in the far-red light condition they exhibit a de-etiolated phenotype. However to my understanding a de-etiolated phenotype has chloroplast stimulation. How then should the WT far-red response phenotype be described so as to differentiate it from other de-etiolated responses such as WT under red light? Would it be enough to say de-etiolated phenotype without chlorophyll production?]]> 2020-04-29 21:42:31 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/540436442 https://padlet.com/biologydept/3170hpkg7utv/wish/541265943 Plants cannot synthesise chlorophyll in FR so they display other de-etiolation phenotypes (short hypocotyl and expanded cotyledons) but will be yellow. If you use the phrase 'de-etiolated phenotype', make sure that you have explained somewhere what this means.]]> 2020-04-30 08:34:05 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/541265943 https://padlet.com/biologydept/3170hpkg7utv/wish/541756180 Is aurea mutant found strictly in tomatos or can it also be observed in Arabidopsis?]]> 2020-04-30 12:28:21 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/541756180 https://padlet.com/biologydept/3170hpkg7utv/wish/543176429 Aurea is a tomato mutant, but there are equivalents in Arabidopsis. These were discussed in my lecture.

]]> 2020-04-30 22:47:25 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/543176429 https://padlet.com/biologydept/3170hpkg7utv/wish/544224660 Why are references included in the word count? I'm struggling to reference my discussion because my reference list eats into it. ]]> 2020-05-01 15:04:45 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/544224660 https://padlet.com/biologydept/3170hpkg7utv/wish/544225499 if two genotypes have the same mutation is it okay if we're less detailed/descriptive about the second genotype when describing it in the results/mutant justification section?

]]> 2020-05-01 15:05:07 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/544225499 https://padlet.com/biologydept/3170hpkg7utv/wish/544368290 I'm possibly not understanding something rather fundamental, if so just ignore my question but I don't understand how to differentiate between hy1 and 2 mutants based on phenotype. Are we supposed to be able to? Or if we think that one of the genotypes could be a hy1/2 mutant do we just put both down?]]> 2020-05-01 16:05:41 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/544368290 https://padlet.com/biologydept/3170hpkg7utv/wish/544580771 i understand that these mutants have decreased chromophore biosynthesis in the chloroplasts but does this also affect the chromophores of cryptochromes? or are the chromophores of cryptochromes made from a different pathway?]]> 2020-05-01 17:47:13 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/544580771 https://padlet.com/biologydept/3170hpkg7utv/wish/547117431 should we give a brief explanation  of the reason behind the length of  the hypocotyl of genotype A in white light in the results section or in discussion?]]> 2020-05-03 16:13:12 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/547117431 https://padlet.com/biologydept/3170hpkg7utv/wish/547593592 This question has been answered in an email to all students.

]]> 2020-05-03 20:54:54 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/547593592 https://padlet.com/biologydept/3170hpkg7utv/wish/547594480 As long as your report clearly explains the phenotypes of a particular genotype, you can refer to this when discussing the same genotype again.

]]> 2020-05-03 20:55:34 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/547594480 https://padlet.com/biologydept/3170hpkg7utv/wish/547598854 These are different mutants but have the same phenotype. It wouldn't be possible to distinguish between them through physical appearance alone.

]]> 2020-05-03 20:58:38 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/547598854 https://padlet.com/biologydept/3170hpkg7utv/wish/547600285 Cryptochromes have different chromphore molecules to phytochromes. These are synthesised via separate pathways.

]]> 2020-05-03 20:59:39 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/547600285 https://padlet.com/biologydept/3170hpkg7utv/wish/547603353 No explanations needed in the justifications section. Just state how phenotypes differ to WT.

]]> 2020-05-03 21:02:00 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/547603353 https://padlet.com/biologydept/3170hpkg7utv/wish/548806171 i am a bit confused by the answer to the previous question stating that no explanations are needed in the justification section as the pdf on blackboard states "The majority of marks for this practical will be given for logical reasoning and clarity of explanation (10 marks per genotype)."]]> 2020-05-04 10:15:11 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/548806171 https://padlet.com/biologydept/3170hpkg7utv/wish/548965776 Apologies for the confusion, I assumed the question was asking whether there needed to be detailed discussion. Mutant identifications need to be clearly justified with regard to the phenotypes in different conditions and known photoreceptor/light signalling component functions but deeper discussion should be left for the discussion section. 


]]> 2020-05-04 11:32:59 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/548965776 https://padlet.com/biologydept/3170hpkg7utv/wish/549278742 On my draft turnitin submission, I had 20% similarity to papers submitted to UOB. I'm guessing that this practical has been ran before, but was wondering if this will effect us at all? I don't want to be penalised for plagiarism, but cannot write my report without using similar language. ]]> 2020-05-04 13:34:56 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/549278742 https://padlet.com/biologydept/3170hpkg7utv/wish/549556202 Do we need to use a specific/journal reference style ?]]> 2020-05-04 14:56:31 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/549556202 https://padlet.com/biologydept/3170hpkg7utv/wish/549929444 This practical has run for 9 years. We don't just look at percentage similarity- there is a second check which will determine whether there are blocks of identical text, or just key word and phrases. We ignore the latter. If you are concerned, please email me and I can have a look over your draft submission and give you my assessment of the Turnitin report.

]]> 2020-05-04 16:49:42 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/549929444 https://padlet.com/biologydept/3170hpkg7utv/wish/549939273 No specific journal style- just be consistent!

]]> 2020-05-04 16:52:51 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/549939273 https://padlet.com/biologydept/3170hpkg7utv/wish/551499617 If I'm starting a sentence with phyA in terms of the holophytochrome, do I need to capitalise the P or can i write the whole thing in lower case?]]> 2020-05-05 09:10:24 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/551499617 https://padlet.com/biologydept/3170hpkg7utv/wish/552023917 2020-05-05 13:17:47 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/552023917 https://padlet.com/biologydept/3170hpkg7utv/wish/552162310 Have noticed a small mistake in my uploaded document and that the re-submission button isn't greyed out like usual. Just double checking it is ok to re-submit for this assignment?]]> 2020-05-05 14:03:11 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/552162310 https://padlet.com/biologydept/3170hpkg7utv/wish/552239056 is it correct to use the term functional redundancy when talking abut the action of two photoreceptors in white light? For example if both photoreceptors have the same action in red and blue light separately and when brought together in white light they would both be controlling the same phenotypes but by being activated by different parts of the white light, can that be called functional redundancy because technically they aren't being activated by the same wavelength of light, but in the situation they are effectively doing the same thing.]]> 2020-05-05 14:27:04 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/552239056 https://padlet.com/biologydept/3170hpkg7utv/wish/552291703 Yes, this is correct terminology. If two photoreceptors both inhibit hypocotyl elongation in white light, then they are functionally redundant for this response in this condition. This doesn't mean that they signal through the same pathway.]]> 2020-05-05 14:43:10 UTC https://padlet.com/biologydept/3170hpkg7utv/wish/552291703