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      <title>Answers to calculations by Usic</title>
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      <language>en-us</language>
      <pubDate>2021-09-28 10:05:57 UTC</pubDate>
      <lastBuildDate>2022-03-22 22:34:48 UTC</lastBuildDate>
      <webMaster>hello@padlet.com</webMaster>
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      <item>
         <title>Answer to Q1</title>
         <author>usictech</author>
         <link>https://padlet.com/usictech/2z9e3ygjolhy7k3l/wish/1516873491</link>
         <description><![CDATA[<div>10 x 25 = [C<sub>2</sub>] x 500, therefore: [C<sub>2</sub>] = (10 X 25) / 500 = 0.5 µg ml and therefore: 0.5 x 1000 = 500 ng ml<sup>-1</sup></div>]]></description>
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         <pubDate>2021-05-12 10:00:51 UTC</pubDate>
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      <item>
         <title>Answers to Q2</title>
         <author>usictech</author>
         <link>https://padlet.com/usictech/2z9e3ygjolhy7k3l/wish/1516875243</link>
         <description><![CDATA[<div>A) The dilution factor for KCl is 20-fold. To prepare the solution, use<strong> 0.050 ml</strong> ( ≡ 50.0 µl) of KCl stock solution, plus <strong>0.950 ml </strong>( ≡ 950.0 µl) of water.<br><br>B) The dilution factor for NaCl is 40-fold and the dilution factor for glucose is 10-fold. To prepare the solution, use <strong>1.25 ml </strong>of NaCl stock solution, <strong>5.00 ml</strong> of glucose stock solution and <strong>43.75 ml</strong> of water.<br> <br>C) The dilution factor for NaCl is 20-fold, the dilution factor for KCl is 80-fold, the dilution factor for CaCl<sub>2</sub> is 4-fold and the dilution factor for glucose is 20-fold. To prepare the solution, use <strong>5.00 ml</strong> of NaCl stock solution, <strong>1.25 ml </strong>of KCl stock solution, <strong>25.00 ml</strong> of CaCl<sub>2</sub> stock solution, <strong>5.00 ml </strong>of glucose stock solution and <strong>63.75 ml </strong>of water.<br><br>D) First, convert the required concentrations to the same units as those of the stock solutions. In this case, µmol ml-1 and mmol l-1 are directly equivalent, (divide by 10<sup>3</sup> to convert from µmol to mmol, then multiply by 10<sup>3 </sup>to convert from ml to l) so the numerical values remain the same. The dilution factor for NaCl is 5-fold, for CaCl<sub>2</sub> it is 8-fold and for KCl it is 10-fold. To prepare the solution, use<strong> 2.00 ml </strong>of NaCl stock solution, <strong>1.25 ml </strong>of CaCl<sub>2</sub> stock solution, <strong>1.00 ml </strong>of KCl stock solution and <strong>5.75 ml</strong> of water.<br><br>E) First, convert the glucose concentration from nmol ml<sup>-1</sup> to mmol l<sup>-1</sup> (divide by 10<sup>6</sup> to convert nmol to mmol and multiply by 10<sup>3 </sup>to convert ml to l), giving glucose at 0.2 mmol l<sup>-1</sup> (all other concentrations are in the same units as those of the stock solutions). The dilution factor for Cacl<sub>2</sub> is 20-fold, for KCl is 4-fold and for glucose is 25-fold. To prepare the solution, use <strong>1.25 ml </strong>of CaCl<sub>2 </sub>stock solution, <strong>6.25 ml</strong> of KCl stock solution, <strong>1.00 ml </strong>of glucose stock solution and <strong>16.50 ml</strong> of water.&nbsp;</div><div><br></div>]]></description>
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         <pubDate>2021-05-12 10:02:08 UTC</pubDate>
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