https://padlet.com/pirta_hotulainen/50ru Tehty onnenpotkuin en-us 2017-09-06 18:08:33 UTC 2025-10-05 18:04:20 UTC hello@padlet.com https://padlet.com/pirta_hotulainen/50ru/wish/185440401 Here we have stainings with phalloidin (left, only filamentous actin) and actin-monomer antibody (right)]]> 2017-09-07 11:00:30 UTC https://padlet.com/pirta_hotulainen/50ru/wish/185440401 https://padlet.com/pirta_hotulainen/50ru/wish/278339596 2018-09-06 10:40:20 UTC https://padlet.com/pirta_hotulainen/50ru/wish/278339596 https://padlet.com/pirta_hotulainen/50ru/wish/278339597 2018-09-06 10:40:20 UTC https://padlet.com/pirta_hotulainen/50ru/wish/278339597 https://padlet.com/pirta_hotulainen/50ru/wish/278339707 In test tube, cofilin depolymerize actin filaments. Can we test if this is cells as well?

In vitro and with test animals too. In vitro would be easier, it might have different effects in animals. And animal models may also have other depolymerizers.

How we shold do it?
How we can manipulate cofiline and how we should analyze cells?

We  GFP label actin and have also control cells. We would use photo activation to separate black and green parts.

We are using 2 groups of cells (neurons) for control and a cofilin inhibited cell: Then, we mesure the depolymerisation  speed in both cells throughout photoactivation with a fluorescent microscope. After that we are doing a statistical calcul to see if the numbers are significantly different and conclude.

How to test whether cofilin contributes to cellular processes?

We would observe the effects in knock-out mice for cofilin. Or we could see what happens in microlevel in cells. 


]]> 2018-09-06 10:40:56 UTC https://padlet.com/pirta_hotulainen/50ru/wish/278339707 https://padlet.com/pirta_hotulainen/50ru/wish/278345490 How to test:
Using mice - 3 genotypes (wild type, knock-out, overexpression)
 3 cell lines
- skeletal muscle, tibia anterioris (whole muscle biopsy)
mmune cells, skin cells]]> 2018-09-06 11:07:05 UTC https://padlet.com/pirta_hotulainen/50ru/wish/278345490