分析与推理 - How should we proceed with the next step of this research? by

Does methyl diet affect the expression of the mice agouti gene?

guying — 2024-05-06 03:35:33 UTC

Knock out the agouti gene

2024-05-16 05:51:54 UTC

DNA sequencing

2024-05-16 05:52:06 UTC

RNA sequencing

2024-05-16 05:52:39 UTC

Study the produced protein and mRNA

yumandanielzhu — 2024-05-16 05:52:43 UTC

Use different diets (for instance, different levels of vietnams) on mice with same agouti genes

2024-05-16 05:52:56 UTC

Detecting for the amount of methyl markers near the agouti gene

2024-05-16 05:53:26 UTC

Investigate step by step correlating with the central dogma

2024-05-16 05:53:42 UTC

Basically first investigate the transcription process, if the mRNA correlates with the expected DNA sequences; then if I didn't find anything I would investigate the translation process and see if the proteome correlates with the transcriptome (using sequencing and stuffs)