Elisa Malagoli- W questions slide 15 by elisamalagoli https://padlet.com/elisa_malagoli/1ifxzmni95k4 en-us 2016-12-05 04:47:13 UTC 2017-03-15 10:46:09 UTC hello@padlet.com Which piece of information can be find out from a DNA pattern bands? elisa_malagoli https://padlet.com/elisa_malagoli/1ifxzmni95k4/wish/141652992 2016-12-05 04:50:23 UTC https://padlet.com/elisa_malagoli/1ifxzmni95k4/wish/141652992 What is the basic principle of gel electrophoresis? elisa_malagoli https://padlet.com/elisa_malagoli/1ifxzmni95k4/wish/141653150 The gel is immersed with an electrophoresis buffer that provides ions to carry a current and another type of buffer to maintain pH in a costant valor.]]> 2016-12-05 04:53:44 UTC https://padlet.com/elisa_malagoli/1ifxzmni95k4/wish/141653150 Why does DNA migrate toward positive electrode? elisa_malagoli https://padlet.com/elisa_malagoli/1ifxzmni95k4/wish/141653395 DNA migrates toward a positive electrode because it has a negative charge.]]> 2016-12-05 04:57:57 UTC https://padlet.com/elisa_malagoli/1ifxzmni95k4/wish/141653395 What is the TBE composition? elisa_malagoli https://padlet.com/elisa_malagoli/1ifxzmni95k4/wish/141653563 The TBE is composed by a solution of Tris-Borato-EDTA, where EDTA takes divalent cations.]]> 2016-12-05 05:00:07 UTC https://padlet.com/elisa_malagoli/1ifxzmni95k4/wish/141653563 Why is important to add Mg ions? Are they co-enzymes or cofactors? elisa_malagoli https://padlet.com/elisa_malagoli/1ifxzmni95k4/wish/141654007 It's a cofactor]]> 2016-12-05 05:08:06 UTC https://padlet.com/elisa_malagoli/1ifxzmni95k4/wish/141654007 How can DNA be detected in agarose gel? elisa_malagoli https://padlet.com/elisa_malagoli/1ifxzmni95k4/wish/141654501 DNA can be dected by a UV-lamp]]> 2016-12-05 05:16:51 UTC https://padlet.com/elisa_malagoli/1ifxzmni95k4/wish/141654501 Why are DNA dyes dangerous for human health? elisa_malagoli https://padlet.com/elisa_malagoli/1ifxzmni95k4/wish/141654682 They are cancerogenic and also cause mutation]]> 2016-12-05 05:19:53 UTC https://padlet.com/elisa_malagoli/1ifxzmni95k4/wish/141654682 What should be done to prepare an agarose gel? elisa_malagoli https://padlet.com/elisa_malagoli/1ifxzmni95k4/wish/141654786 Mix agarose gel with electrophoresis buffer and melt it in microwave, then let it solidificate on room temperature. put the gel in electrophoresis chamber, paying attention to not rip the gel.]]> 2016-12-05 05:21:45 UTC https://padlet.com/elisa_malagoli/1ifxzmni95k4/wish/141654786 What should not be done preparing an agarose gel? elisa_malagoli https://padlet.com/elisa_malagoli/1ifxzmni95k4/wish/141656021 Preparing agarose gel need attention, it's necessary wear gloves and protective glasses, beacuse it's vey hot (and also can boil) when is just melted. Important is not touch the gel beacuse is cancerogenic and can provocate mutation. Also, for the right way to do the DNA analysis, agorose gel has to be treat with a lot of attention and care.]]> 2016-12-05 05:49:31 UTC https://padlet.com/elisa_malagoli/1ifxzmni95k4/wish/141656021 Correzione valeria_fregni https://padlet.com/elisa_malagoli/1ifxzmni95k4/wish/147230364 Hai risposto correttamente alle domande sull'elettroforesi.
Bene. VF P.S. si poteva mettere tutto in un unico padlet.]]> 2017-01-15 12:08:39 UTC https://padlet.com/elisa_malagoli/1ifxzmni95k4/wish/147230364