A2459C Lesson 03 / A2469C Lesson 12 by Jia Ramchandani https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi Some key points en-us 2021-12-06 12:49:16 UTC 2023-07-11 09:56:53 UTC hello@padlet.com EXFOLIATIVE CYTOLOGY jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1930993537 2021-12-06 12:49:16 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1930993537 Obtained by SPONTANEOUS EXFOLIATION into a body fluid --> Cells have been spontaneously shed by the body jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1930993539 2021-12-06 12:49:16 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1930993539 Obtianed by PHYSICAL / MECHANICAL EXFOLIATION jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1930993540 2021-12-06 12:49:16 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1930993540 Cells --> Transferred to a glass slide --> visualized under a microscope jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1930993541 2021-12-06 12:49:16 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1930993541 Pathologist will examine the cells and determine if they are BENIGN or MALIGNANT jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1930993542 2021-12-06 12:49:16 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1930993542 CYTOLOGY TECHNIQUE: jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1930993543 1. Quick aspiration (avoid blood clot)
2. Quick transfer of material onto the glass slides
3. Proper smearing (avoid crushing the cells)
4. Immediate fixation (avoid air drying of specimen )
5. Stain with cytological stain like Pap stain (For alcohol fixed slide) 
6. Stain with Romanowsky Stain or DiffQuik (For air dried slide)
7. Cell Block preparation (optional step)

]]> 2021-12-06 12:49:16 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1930993543 jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1930993546 2021-12-06 12:49:16 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1930993546 Brushings and Fluids and FNAC jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1930993547 2021-12-06 12:49:16 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1930993547 DIRECT SMEARS jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1930993548
- AIR-DRIED (In the lab, these air-dried smears are rehydrated by placing in normal saline for 30 seconds, and then they are fixed in 95% alcohol, and then stained with Romanowsky Stains like MGG, Leishman or DiffQuik, or other special stains)
- ALCOHOL-FIXED / WET FIXED smears (Smears immediately fixed with cytology spray fixative or immerse in 95% Ethyl Alcohol for at least 20 minutes. Stained with Pap stain)]]> 2021-12-06 12:49:16 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1930993548 LIQUID BASED CYTOLOGY (LBC) jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1930993549 - SurePath or ThinPrep]]> 2021-12-06 12:49:16 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1930993549 CELL BLOCK (Optional) jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1930993550 2021-12-06 12:49:16 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1930993550 jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1930993553 2021-12-06 12:49:16 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1930993553 jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1930993554 2021-12-06 12:49:16 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1930993554 CYTOLOGY --> Study of CELLS jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1931002940 2021-12-06 12:54:20 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1931002940 Naturally passed and freely available for collection jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1931015541 E.g. sputum, urine]]> 2021-12-06 13:00:11 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1931015541 Require active drainage jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1931019462 E.g. Serous fluids, CSF]]> 2021-12-06 13:02:18 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1931019462 Induced by instrumentation jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1931028453 E.g. cells are collected by scraping, swabs, brushing or washing from  tissue surfaces. ]]> 2021-12-06 13:06:57 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1931028453 jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1931067106 2021-12-06 13:24:59 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1931067106 jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1931073555 Gelatin foam cell blocks made from cytology fluid specimens]]> 2021-12-06 13:27:54 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1931073555 jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1931076746 2021-12-06 13:29:15 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1931076746 CYTOSPINS (for Body Fluids) jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1931129245 2021-12-06 13:50:27 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1931129245 jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1931158251 2021-12-06 14:01:37 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1931158251 jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1931161949 2021-12-06 14:03:02 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1931161949 jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1931221422 2021-12-06 14:24:56 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/1931221422 jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/2642632695 2023-07-11 09:44:21 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/2642632695 jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/2642634634 2023-07-11 09:49:01 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/2642634634 jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/2642634947 2023-07-11 09:49:52 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/2642634947 jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/2642636187 2023-07-11 09:53:00 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/2642636187 jia_ramchandani2_2 https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/2642636471 2023-07-11 09:53:46 UTC https://padlet.com/jia_ramchandani2_2/12rqm39i06i6urzi/wish/2642636471