1 by Keerthi Sivan https://padlet.com/ksivan1_314/1144ltqbjxv6 Made with a bold sensibility en-us 2018-10-19 10:52:21 UTC 2026-02-22 13:07:10 UTC hello@padlet.com 1 b) lydiafreeman97 https://padlet.com/ksivan1_314/1144ltqbjxv6/wish/294752133 Lydia's answer:
"ADP.BeF3is an ATP analog, the binding of ADP.BeF3to ATPase receiver domain to ensure ATPase remains in the ground state and cannot undergo hydrolysis. MST is microscale thermophoresis which monitors changes in the hydration shell in conditions close to that in the cell. The data suggest it is useful to demonstrate the similarity of cooperativity in ADP.BeF3being almost the same as that of the apo enzyme with both values being 1.6. However ADP.BeF3has a significantly higher binding affinity than the apoenzyme as it requires a lower concentration, approximately 42nM less, for ½ of the substrate to bind suggesting that whilst ADP.BeF3bound INO80 is less active than INO80 it still is more reactive that the apoenzyme.

 

https://doi.org/10.1016/j.str.2007.02.007

https://www.biozentrum.unibas.ch/uploads/media/Microscale_Thermophoresis_Intro.pdf
"
]]> 2018-10-19 10:56:08 UTC https://padlet.com/ksivan1_314/1144ltqbjxv6/wish/294752133 1b) ellahallyounger97 https://padlet.com/ksivan1_314/1144ltqbjxv6/wish/294752400 ADP.BeF3 tightens nucleosome binding. ]]> 2018-10-19 10:57:47 UTC https://padlet.com/ksivan1_314/1144ltqbjxv6/wish/294752400 1b) jeanne_pigeon98 https://padlet.com/ksivan1_314/1144ltqbjxv6/wish/294752924 Dissociation constant: when half the protein in bound 

]]> 2018-10-19 11:00:28 UTC https://padlet.com/ksivan1_314/1144ltqbjxv6/wish/294752924 1a) ellahallyounger97 https://padlet.com/ksivan1_314/1144ltqbjxv6/wish/294753380 thermophilum is more stable
paper E - model building and refinement was done iteratively from already known high resolution homology models]]> 2018-10-19 11:02:37 UTC https://padlet.com/ksivan1_314/1144ltqbjxv6/wish/294753380 1a) jeanne_pigeon98 https://padlet.com/ksivan1_314/1144ltqbjxv6/wish/294753391  
Paper E uses fungus Chaetomium thermophilum and has higher definition 
Paper A uses human complex 
 
Paper E has acces to way more protein, and you need to average the data from all the protein, therefore higher resolution 
Fungus is easier to purify, therefore will have to go through fewer steps of purification, and will be better quality 
 
Also its heat stable, and recombinant protein with conserved subunits between yeast and humans 

]]> 2018-10-19 11:02:40 UTC https://padlet.com/ksivan1_314/1144ltqbjxv6/wish/294753391 1c) jeanne_pigeon98 https://padlet.com/ksivan1_314/1144ltqbjxv6/wish/294754076 260 is for DNA, 280 is for proteins 

]]> 2018-10-19 11:06:17 UTC https://padlet.com/ksivan1_314/1144ltqbjxv6/wish/294754076 1 C) https://www.ogt.com/resources/literature/483_understanding_and_measuring_variations_in_dna_sample_quality hannah_martin11 https://padlet.com/ksivan1_314/1144ltqbjxv6/wish/294754741
How phenol affects absorbance ratios]]> 2018-10-19 11:08:34 UTC https://padlet.com/ksivan1_314/1144ltqbjxv6/wish/294754741 1c) jeanne_pigeon98 https://padlet.com/ksivan1_314/1144ltqbjxv6/wish/294756634
tryp 415
tyrosine : 1642
7560 total 
2057 phenol

27.2%  Is phenol

do DNA binding proteins usually have a higher percentage of phenol amino acids?

what percentage of the complex is DNA? 
 
]]> 2018-10-19 11:16:53 UTC https://padlet.com/ksivan1_314/1144ltqbjxv6/wish/294756634 1c) jeanne_pigeon98 https://padlet.com/ksivan1_314/1144ltqbjxv6/wish/294757408  The peak at lower wavelengths is caused by absorbance of peptide and carboxylic acid moieties in the compounds.

]]> 2018-10-19 11:20:40 UTC https://padlet.com/ksivan1_314/1144ltqbjxv6/wish/294757408 1c) jeanne_pigeon98 https://padlet.com/ksivan1_314/1144ltqbjxv6/wish/294766883 2 DNA chains and 10 protein chains ]]> 2018-10-19 11:49:51 UTC https://padlet.com/ksivan1_314/1144ltqbjxv6/wish/294766883 1a ellahallyounger97 https://padlet.com/ksivan1_314/1144ltqbjxv6/wish/295013639 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4706439/ ]]> 2018-10-19 21:10:44 UTC https://padlet.com/ksivan1_314/1144ltqbjxv6/wish/295013639