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Macbeth is the protagonist of the story. In the beginning, he is the Thane of Glamis, but the witches tell him that he will be declared the King. Through Lady Macbeth, he is convinced to kill King Duncan, and therefore become King.

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Malcolm is King Duncan's eldest son and is declared as Duncan's heir. Donalbain is King Duncan's second son. After Duncan's death, he is the one who suggests Malcolm flee to Ireland and go their separate ways so that they do not die. Due to this, the 2 sons become prime suspects in the murder of Duncan. Later Malcom is seen in England hiding with Macduff and together, the 2 of them are planning to take over Scotland from Macbeth.

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King Duncan is the king of Scotland. He is a good and virtuous king. He has always been nice to Macbeth, but due to Macbeth's desire to be King, Duncan gets murdered while he is asleep at Macbeth's castle.

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Lady Macbeth is the wife of King Duncan. She is a strong, rutheless and ambition lady. She is the one who suggested Macbeth to kill Duncan, and is the one who helped him carry out the murder. After the murder, she is constantly reassuring and calming Macbeth down, and helping him cover up the muder.

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The 3 witches are women and are separately distinguished from the other characters through the way they dress and speak. These 3 women create spells through chants and cast these dark spells to make people, animals, and objects die and threaten the earth. These witches also can tell the fate of people and tell Banquo and Macbeth their fates.

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Banquo is a thane in Duncan's army and at first Macbeth's noble and best friend. Banquo goes to the 3 witches to ask them of his prophecy and they tell him that his children will rule Scotland. However, he does not realize this means that he has to die for his children. Macbeth fearing that Banquo will take over the kingdom, he orders murderers to kill him. Fearing being killed, Fleance runs away.

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Macduff is a thane and loyal to Duncan and Malcolm. After Duncan's body is found by him, he suspects Macbeth for being responsible. Macbeth realizing Macduff to not be on his side, arranges for Macduff and his family to be killed, however Macduff had already fled to England to get help from the King to fight Macbeth.

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Fleance is the son of Banquo. Macbeth sees him as a threat and orders the killing of him and his father. Fleance survives and flees.

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The 3 murderers from an order from Macbeth are responsible for the killing of Banquo and his son. However, they do not fully succeed because Banquo's son escapes.

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Hecate is the leader of the witches and shows Macbeth his prophecy and destiny.

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Siward is the Earl of Northumberland and is Malcolm's uncle. Siward leads the troops into battle to get back Malcolm's throne. At the end of the battle, Siward's son dies and Siward is proud that he died in battle, as a proud soldier.

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Ross is Macduff's cousin and a loyal Scottish nobleman. He is a messenger to Macbeth, Macduff and Siward.

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Lennox is a Thane and accompanies Duncan. When Duncan dies, Lennox is with Macduff and says he cannot rememeber what happened. As the play goes on, Lennox is convinced Macbeth is guilty for killing Duncan.

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Seyton is the servant of Macbeth when everyone else has abandoned him. He delivers news to Macbeth and informs him of Lady Macbeth's death.

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The Doctor has been Macbeth's personal doctor for a while, and is loyal and concerned about Lady Macbeth and her health. He consults the Gentlewomen and uses her help to diagnose the Lady.