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You will need:
-2 droppers
-1 oz of water
-1 oz of hydrogen peroxide
-One test tube with cm markings (labeled Test Tube 5)
-Tweezers
-Notebook and pencil
-Boiled liver

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Step 1: Using your dropper, add 2 cm of water inside Test Tube 5.
Step 2: Using your tweezers,pick up the boiled liver and place t in Test Tube 5. Set the tweezers off to the side.
Step 3: Use a popsicle stick or skewer to gently push the liver down into the tube until  it touches the bottom of the tube.
Step 4: Using your dropper, add 2 cm of hydrogen peroxide to Test Tube 5
Step 5: Record your observations. What happened? Did you see a reaction take place? If so, what kind of reaction did you see?

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The results for this experiment are shown below. The x-axis represents each Test Tube. The Y-axis represents the scale of the reaction. If the Test Tube had a level of 0, that would mean that there was no reaction. 1 means little reaction, 2 means some reaction, 3 means that there was a reaction, 4 represents a big reaction, and 5 represents an "explosive" reaction.

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Enzymes are small proteins. Their job is to break down substrates. When enzymes break down these substrates, often, a chemical reaction is produced. In this experiment, the enzymes were contained inside the piece of liver, and the substrates were contained inside the hydrogen peroxide. So when the liver came into contact with the hydrogen peroxide, it produced a reaction. This is because the enzymes in the liver were breaking down the substrates in the hydrogen peroxide into oxygen and water. You could definitely see the water, but how could you be sure that the gas produced was really oxygen? This is why the piece of hot red wood was held over Test Tube 4. When enzymes are heated (in this case, by the piece of hot wood), their reaction rate speeds up. When the reaction rate is sped up, it creates speed. When molecules are moving under speed, they move faster and produce more heat. In addition to this, fire also needs oxygen to survive. Without oxygen, a fire will die. This is why the wood caught fire when it was pressed against the mixture in the top. The mixture of heat and oxygen coming from the mixture allowed the wood to reignite and catch fire. Now fast forward to Procedure II. This optional experiment allowed you to see how optimal heat affects enzymes. When the liver was boiled, it was heated. Thus, the enzymes inside the liver were also heated. Now, earlier you saw how enzymes worked faster when the heat was raised. However, when the hydrogen peroxide was added to Experiment II, no reaction took place. This is because of how enzymes interact with optimal heat. As the heat increases, enzymes work faster. However, when the temperature is raised to 40 degrees Celsius or above, the rate of reaction begins to slow down. This is because the enzyme starts to lose its shape, much like how ice starts to melt after 0 degrees Celsius. And because the substrate is no longer able to fit QUITE RIGHT into the active site of the enzyme, the reaction rate is slowed down, and even completely stops at a certain point. This is seen in Test Tube 5, when there is no reaction, simply because the boiled liver raised the temperature to a point in which the enzymes were no longer able to function properly.

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Step 1: Using your dropper, add 2 cm of water to Test Tube 1.
Step 2: Using your tweezers, pick up the liver and place it in Test Tube 1. Set the tweezers off to the side.
Step 3: Use a popsicle stick or skewer to gently push the liver down into the tube until it touches the bottom of the tube.
Step 4: Record your observations. What happened? Did you see a reaction take place? If so, what kind of reaction did you see?
Step 5: Using your dropper, add 2 cm of hydrogen peroxide to Test Tube 2
Step 6: Using your tweezers, pick up another piece of liver and place it is Test Tube 2. Set the tweezers off to the side.
Step 7: Use a popsicle stick or skewer to gently push the liver down into the tube until it touches the bottom of the tube.
Step 8: Record your observations. What happened? Did you see a reaction take place? If so, what kind of reaction did you see?
(You should have noticed that Test Tube 2 had a reaction, whereas Test Tube 2 did not. Now, you must prove that the reaction in Test Tube 2 contains oxygen.)
Step 9: Using your dropper, add 2 cm of hydrogen peroxide to Test Tube 3.
Step 10: Using your matches or lighter, light a wooden splint on fire.
Step 11: Wait for the flame to go out. You should see a glowing red tip where the flame once was. Drop the wooden splint into Test Tube 3.
Step 12: Record your observations. What happened? Did you see a reaction take place? If so, what kind of reaction did you see?
Step 13: Using your dropper, add 2 cm of hydrogen peroxide to Test Tube 4.
Step 14: Using your tweezers, pick up the liver and place it in Test Tube 4. Set the tweezers off to the side.
Step 15: Use a popsicle stick or skewer to gently push the liver down into the tube until it touches the bottom of the tube.
Step 16: Record your observations. What happened? (You should have seen the same reaction take place that happened in Test Tube 2.)
Step 17: Using your matches or lighter, light a wooden splint on fire.
Step 18: Wait for the flame to go out. You should see a glowing red tip where the flame once was.
Step 19: Gently touch the wooden splint to the bubbles on top of Test Tube 4.
Step 20: Pull the wooden splint away after about 3 seconds.
Step 21: Record your observations. What happened? Did you see a reaction take place? If so, what kind of reaction did you see?
Step 22: What can be proved from this experiment? Was your hypothesis right, or wrong? What have you learned following this experiment?

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You will need:
-2 droppers
-4 test tubes with cm markings (Labeled Test Tube 1, Test Tube 2, Test Tube 3, and Test Tube 4)
-2 popsicle sticks/skewers
-6 oz of hydrogen peroxide
-1 oz of water
- A box of matches or a lighter
-4 liver pieces
-2 wooden splints
-A notebook and pencil

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The liver contains enzymes. The purpose of this lab is to see if the enzymes in the liver can successfully break down hydrogen peroxide into water and oxygen.

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Can the liver break down hydrogen peroxide?