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Bullets fired from the same weapon carry distinct markings related to the barrel of the gun. In a case where a victim is shot, forensic scientists are able to use an SEM to determine the type of gun the bullet was shot from, thus narrowing the list of suspects.

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SEM's are used for gunshot residue analysis, identification of firearms, investigation of gemstones, examination of paint particles, and filament bulb investigations. It can also be used to examine handwriting, counterfeit money, and non-conducting materials (such as fibers)

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SEM's are often used with TEM's (Transmission Electron Microscope). The TEM is able to identify time of death from a very thin specimen.

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SEM's can magnify the surface of solid objects up to 200,000 times.