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1. To observe the growth of bacteria.
2. To identify if there is any contimination while streaking the bacteria to an agar plate.

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Before mid sem break.

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1. sterile microcentrifuge  tube was used to fill a liquot of 500 ꭎl of sterile 30% glycerol.

2.  500ꭎl of bacterial culture was added to the tube and vortex mixer was used to mix with the glycerol.

3. The organism name,strain,date and etc was label the tube .

4. Recorded the location and the tube was placed in the freezer (-20℃).

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After mid sem break

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5. A small volume of active bacteria culture was removed from the tube. The tube was open and frozen culture was scrape with a sterile pipette tip. The small volume of frozen/thawed cell was streak to an agar plate.

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The active culture

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Heat the loop wire to disinfect

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The active culture was take out to streak to an agar plate.

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Streaking time!

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6. The vialibility of the culture was check after 24 hours of incubation at 37℃

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From the photo above, Bacillus cereus is a Gram-positive and Escherichia coli is a Gram-negative. Both of this bacteria is a rod-shaped. Gram positive bacteria will retain crystal violet dye and stain dark violet or purple, its remain coloured blue or purple with gram stain when washed with absolute alcohol and water while for gram negative an be decolourized to accept counter stain (Safranin or Fuchsine); stain red or pink, they don't retain the Gram stain when washed with absolute alcohol and acetone.