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If it is heaven, must find it out tonight

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Towards the end of the play, Macbeth becomes more and more assertive in his actions and decides to kill Fleance. However, only now does Macbeth realize that he has walked too far in the "river of blood" that even if he turns around, his sins will not be forgiven. Moreover, Macbeth's innate desires for the thrown is too strong in that he no longer needs any external reinforcement and persuasion--Lady Macbeth's influence.

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I am in blood

Stepped in so far that, should I wade no more,

Returning were as tedious as go o'er.

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When Macbeth's away, Lady Macbeth suffers from guilt and regret of what she's done. This is quite contrasting from how she was like to Macbeth when he saw the ghost in Act 3 Scene 4.(Macbeth talking to invisible ghost=Lady Macbeth trying to wash off invisible bloodstain)

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Macbeth is becoming more secretive around Lady Macbeth, making him a more dominant character as it shows he has more power over controlling what she knows and his plans.

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"Be innocent of the knowledge, dearest chuck,
Till thou applaud the deed

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Difference in public and private relationship 

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Difference in public and private relationship 

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When Macbeth speaks to the invisible ghost of Banquo, Lady macbeth makes excuses for her husband in front of others. But when she speaks to Macbeth, she scolds him questioning his manhood

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"My lord is often thus

And hath been from his youth. Pray you, keep seat.

The fit is momentary; upon a thought

He will again be well. If much you note him,

You shall offend him and extend his passion.

Feed and regard him not. (aside toMACBETH) Are you a man?"

"You have ruined our good cheer and disrupted the gathering by making a spectacle of yourself

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Shows that Macbeth is becoming more independent from Lady Macbeth, as he does not consult her when he plans to kill Banquo. This makes him the more dominant figure in their relationship, as he is taking more responsibility and action in getting what he wants.

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Macbeth tells his wife that he has planned “a deed of dreadful note” for Banquo and Fleance and urges her to be jovial and kind to Banquo during the evening’s feast, in order to lure their next victim into a false sense of security

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"I'll call upon you straight; abide within.
It is concluded. Banquo, thy soul's flight,
If it is heaven, must find it out tonight

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"Here's the smell of the blood still; all the perfumes of Arabia will not sweeten this little hand."

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"How now, my lord! Why do you keep alone,

Of sorriest fancies your companions making"

"What’s to be done?
Be innocent of the knowledge, dearest chuck,

Till thou applaud the deed. Come, seeling night"

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Change in relationship between Macbeth and Lady Macbeth