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P1.1-106 Investigating the involvement of tomato rhizobacteria in resistance to bacterial wilt Jaehyo PARK (Seoul - REPUBLIC OF KOREA)

P1.1-045 Using bacteriophages as evolutionary tools to control bacterial wilt disease: plant transcriptomic response to phage-resistant bacteria | Sara FRANCO ORTEGA (York - UNITED KINGDOM)

P1.1-088 Multi-transcriptome analysis to elucidate the flavobacterium-mediated suppression of bacterial wilt and the causative bacterium | Soon-Kyeong KWON (Jinju - REPUBLIC OF KOREA)

P2.1-002 The Ralstonia pseudosolanacearum type iii effector ripl delays flowering and promotes susceptibility to pathogen in plants | Wanhui KIM (Seoul - REPUBLIC OF KOREA)

P2.1-019 Contact dependent inhibition in Ralstonia pseudosolanacearum | Rekha GOPALAN NAIR (Tübingen - GERMANY)

P2.1-062 Ralstonia tale-like proteins target host arginine decarboxylase genes and can act as hooks for plant resistance against bacterial wilt disease | Niels GALLAS (Tübingen - GERMANY)

F2.1-2 - Sa-independent mechanism in the tomato diageotropica (DGT) mutant enhance root-mediated resistance to Ralstonia solanacearum k60 Katherine RIVERA-ZULUAGA (Purdue University, West Lafayette - UNITED STATES)

P2.3-022 Emergence and evolution of a novel lineage of Ralstonia solanacearum with expanded host range Daria EVSEEVA (Tübingen - GERMANY)

P3.6-003 Developing phage therapy to reduce plant pathogen virulence in Ralstonia solanacearum Daniel NARINO ROJAS (York - UNITED KINGDOM)

P5.5-002 Find the needle in the haystack - identification of the immune receptors required for the recognition of Ralstonia solanacearum effectors in nicotiana benthamiana | Cecile SEGONZAC (Seoul - REPUBLIC OF KOREA)

P6.1-027 Diversity of strains of Ralstonia solanacearum species complex in benin in west Africa Sanju KUNWAR (Gainesville - UNITED STATES)

P6.1-084 N-acetylcysteine use for bacterial wilt of potatoes and bacterial canker of grapevine Simone PICCHI (Cordeiropolis - BRAZIL)

P8.3-001 Contribution of the quorum sensing of Ralstonia pseudosolanacearum strain oe1-1 to its infection in tomato roots and virulence | Yasufumi HIKICHI (Nankoku - JAPAN)

P8.3-002 Plant signals that induce the hrp regulon in Ralstonia solanacearum and the cognate r. solanacearum receptors Ohnishi KOUHEI (Kochi - JAPAN)

P8.3-003 Coexpression network analysis to understand the quorum sensing-dependent gene regulation mechanism in Ralstonia pseudosolanacearum strain oe1-1 | Masayuki TSUZUKI (Kochi - JAPAN)

P8.3-004 The tomato p69 serine proteases play a role in resistance to bacterial wilt | Weiqi ZHANG (Cerdanyola Del Vallès - SPAIN)

P8.3-005 Ferric uptake regulators, fur1 and fur2, affect the expression of quorum sensing-regulated genes in the conditions with and without ferrous iron in Ralstonia pseudosolanacearum strain oe1-1 | Sora TATEDA (Kochi - JAPAN)

P8.3-006 Selective isolation of Ralstonia solanacearum on msmsa in a saprophytic context Maria BERGSMA-VLAMI (Wageningen - NETHERLANDS)

P8.3-007 Comparison of rhizospheric bacterial communities of potato genotypes with diverse defense responses against Ralstonia solanacearum | Virginia FERREIRA (Montevideo - URUGUAY)

P8.3-008 Application of recombinant internal control for increasing qpcr reliability in the quantitative detection of Ralstonia solanacearum species complex (rssc) in soil samples | Li-Qun ZHANG (Beijing - CHINA)

P8.3-009 First report of Ralstonia pseudosolanacearum on boesenbergia rotunda from thailand Andrew ASPIN (Sand Hutton - UNITED KINGDOM) P8.3-010 Variety of pathogenicity of Ralstonia solanacearum strains | Wlodzimierz PRZEWODOWSKI (Bonin - POLAND)

P9.1-011 Genome editing of banana for resistance to bacterial wilt disease | Leena TRIPATHI (Nairobi - KENYA)

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Tuesday 22nd

•14:00 – 14:20 à C3.6-1 - Ecology and evolution of phage-bacteria interactions drive bacterial wilt disease dynamics Ville-Petri FRIMAN (University of Helsinki, Helsinki - FINLAND)  (Bellecour 1)

Thursday 24th

•09:40 àC5.7-5 - Can temperature adaptation drive Ralstonia solanacearum range expansion in the future? Karla CARDENAS GOMEZ (UNIVERSITY OF YORK, York - UNITED KINGDOM) (Bellecour 2)

• 18:00 - 20:00àC8.3 - Latest advancements in knowledge and management of Ralstonia species (Tête d’Or 1-2)

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Travel award for Sara Franco Ortega to participate in ICPP

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https://www.york.ac.uk/biology/centrefornovelagriculturalproducts/

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Partnerships

We work closely with a broad range of partners, including industry, government and charitable organisations, to deliver scientific breakthroughs and impact.

We have worked with organisations such as GlaxoSmithKline, The Department of Food and Rural Affairs and the UK Ministry of Defence.
Work with us

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We use cutting-edge research to harness the power of nature for the development of new products and processes.

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Thank you to all fantastic speakers and ECRs for being part of this day!

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Author contact: FRANCO ORTEGA Sara (sara.francoortega@york.ac.uk)

Using bacteriophages as evolutionary tools to control bacterial wilt disease: plant transcriptomic response to phage-resistant bacteria

FRANCO ORTEGA S. (1), FIELDS B. (2), MIKONRATA L. (2), FRIMAN V. P. (3), HARPER A. (1,2)

(1) CNAP, Department of Biology, University of York, York, UK; (2) Department of Biology, University of York, York, UK; (3) Department of Microbiology, University of Helsinki, Helsinki, Finland

Text
Ralstonia solanacearum causes disease in more than 200 plant species including bacterial wilt of tomatoes and brown rot of potatoes. This bacterium is part of the EPPO A2 list of quarantine pathogens due to its soilborne and waterborne nature, worldwide distribution and lack of effective control measures. The use of bacteriophages as biocontrol agents is promising, as they exhibit high specificity to individual bacterial species, and do not any affect eukaryotic cells. However, one aspect of the use of phage should not be underestimated: the quick ability of the bacteria to become resistant to the phage. This often leads to a trade-off, whereby in becoming resistant, bacteria lose virulence. This may be exploited to reduce the overall pathogenicity of Ralstonia populations, and can also help us to better understand plant-pathogen interactions. We have successfully identified phage which are effective at controlling R. solanacearum, and are now focused on understanding the transcriptome response of a susceptible tomato cultivar over time when inoculated with either a virulent ancestral strain, in-vitro evolved strains (non-virulent), or an ancestral strain plus phage treatment, which models co-evolution in the soil. The transcriptomic analysis reveals that there are clear differences in the immune response of the plant in each of the treatments. This work sheds light on the key genes activated in susceptible plants when phage resistance mutations occur in the bacteria.

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Author contact: ZHANG Weiqi Zhang (weiqi.zhang@cragenomica.es)

Role of PR proteins in tomato defence against Ralstonia solanacearum

ZHANG W. (1), PLANAS-MARQUÈS M. (1,2), COLL N. S. (1), VALLS M. (1,2)

(1) Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB, Bellaterra, Catalonia, Spain; (2) Department of Genetics, Microbiology and Statistics, Universitat de Barcelona, Barcelona, Catalonia, Spain

Text
Through proteomic analysis of tomato xylem and apoplast we have identified various PR proteins as specifically activated upon Ralstonia solanacearum infection in the resistant cultivar Hawaii7996.

We will present data on the characterisation of the PR-1 and P69 protein families and their roles in tomato defence to bacterial wilt.

Regarding PR-1, we chemically synthesised the CAPE peptide,derived from PR-1b and show that it can limit multiplication of R. solanacearum in tomato plants. We are also generating PR1 CRISPR mutant and overexpression lines with or without CAPE peptide and purifying these proteins to test their role against R. solanacearum in vitro and in planta.

The P69 serine proteases were purified by heterologous expression and their cleavage specificity was defined. We showed that P69D prodomain removal occurred in an autocatalytic and intramolecular reaction. Moreover, overexpression of P69B, D and G limited multiplication of R. solanacearum, while P69C caused cell death in tomato. Finally, a P69D CRISPR loss-of function mutant made resistant tomato Hawaii 7996 more susceptible to R. solanacearum but not to other vascular pathogens.

Our results demonstrate a role for several PR proteins in plant resistance to bacterial wilt.

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Author contact: GOPALAN NAIR Rekha (rekha.gopalan-nair@tuebingen.mpg.de)

Contact dependent growth inhibition in Ralstonia pseudosolanacearum

GOPALAN NAIR R. (1), EVSEEVA D. (1), MCCANN H. (1)

(1) Max Planck Institute for Biology, Tübingen, GERMANY

Text
Ralstonia pseudosolanacearum is a deadly bacterial plant pathogen known to infect many plant species. Comparative genomics of a South Korean population of R. pseudosolanacearum revealed that recombination frequently targets secreted gene products including type III secreted effectors and CdiA proteins, similar to hemagglutinin. CdiA proteins vary in the presence of a C-terminal toxin domain that inhibits the growth of adjacent cells lacking cognate immunity protein CdiI, a phenomenon called contact-dependent growth inhibition (CDI). CDI mediates both antagonistic and cooperative contact-dependent interactions, contributing to the formation of populations sharing identical CDI loci. R. pseudosolanacearum carries an expanded set of CDI loci compared to Burkholderia spp., where their function is better characterized. My work examines whether these loci mediate contact-dependent interactions and contribute to phenotypes associated with virulence and cooperative behavior in R. pseudosolanacearum.

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Author contact: FERREIRA Virginia (virginiaferreira20@gmail.com)

Comparison of rhizospheric bacterial communities of potato genotypes with diverse defense responses against Ralstonia solanacearum.

FERREIRA V. (1,2), TOURNE F. (2), EASTMAN I. (1), RODRÍGUEZ-ESPERÓN C. (1), RODRÍGUEZ G. (3), GONZÁLEZ M. (3), MURCHIO S. (3), DALLA RIZZA M. (3), VILARÓ F. (6), GALVÁN G. (6), GAIERO P. (6), LARAMA G. (4), GONZÁLEZ M. (5), PLATERO R. (1), SIRI M. (2)

(1) Instituto de Investigaciones Biológicas Clemente Estable , Montevideo, URUGUAY; (2) College of Chemistry, Universidad de la República, Montevideo, URUGUAY; (3) National Research Institute for Agriculture , Montevideo, URUGUAY; (4) Biocontrol Research Laboratory, Universidad de La Frontera, Temuno, CHILE; (5) Research Center for Arid Zones, Universidad de La Serena, La Serena , CHILE; (6) College of Agriculture, Universidad de la República, Montevideo, URUGUAY

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Potato (Solanum tuberosum) is one of the most important widespread hosts of Ralstonia solanacearum, the causal agent of bacterial wilt. In Uruguay, germplasm with resistance to R. solanacearum has been identified and advanced clones with different responses to R. solanacearum infection were selected and characterized. Previous results showed that plant resistance was correlated with differential bacterial colonization patterns and induced defense responses after infection. The aim of this work is to study the correlation between plant resistance and rhizosphere microbiome in selected genotypes with different responses to bacterial wilt. Plants were grown in a macrotunnel greenhouse with soil collected from a potato field. Pathogen colonization effects on rhizosphere microbiota were evaluated in healthy and infected plants. Disease progression was recorded and pathogen was quantified in rhizosphere samples. A resistant genotype showed delay in pathogen colonization and high final pathogen concentration in the rhizosphere, comparable to the susceptible genotype. These results suggest that the resistant genotype restricts rhizosphere pathogen colonization, preventing root and stem infection. Bacterial community composition is being analyzed in 76 samples comparing the sequence of V3-V4 region of 16S rARN. It is expected to identify potentially beneficial microbial groups related with resistant plants, contributing to an integrated disease control.

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Author contact: GORTER Florien (florien.gorter@wur.nl)

Host range of a new Ralstonia pseudosolanacearum (phylotype i) strain detected in Dutch surface waters and bittersweet

GORTER F. (1), VOGELAAR M. (1,2), LOMBAERS C. (1), POLEIJ L. (1), MENDES O. (1), BERGSMA-VLAMI M. (2), VAN DER WOLF J. (1)

(1) Wageningen University and Research, Wageningen, NETHERLANDS; (2) Dutch Food and Consumer Product Safety Authority, Wageningen, NETHERLANDS

Text
Species of the Ralstonia solanacearum species complex (RSSC) cause bacterial wilt and are aggressive, contagious and persistent. As such, all species within the RSSC, including Ralstonia pseudosolanacearum (phylotype I), have a quarantine status. Until recently, R. pseudosolanacearum (phylotype I) was only sporadically found within the European Union, and the pathogen was absent from the aquatic environment within this region. However, starting from 2020, the bacterium has repeatedly been found in Dutch surface waters, as well as in bittersweet, a known host of R. solanacearum (phylotype II). Here, we used one of the original isolates from 2020 to determine the host range of this novel pathogen. We introduced a rifampicin resistance marker via homologous recombination and used the transformed strain to inoculate 24 different plant species from the Solanaceae, Rosaceae, and other plant families that are known or suspected hosts of the RSSC. Both cultivated crops and weeds were included in the assays. Young plants were stem inoculated, grown under controlled conditions and monitored for 8 weeks. In case of symptomatic or asymptomatic infection, the assays were repeated using soil inoculation. Research was supported by the Dutch Food and Consumer Product Safety Authority, Naktuinbouw, the Nederlandse Algemene Keuringsdienst, Glastuinbouw NL (Stichting Kennis in je Kas), HZPC, BO Akkerbouw, Stichting Aardbeionderzoek, Deliflor Chrysanten and the Nederlandse Aardappelorganisatie.

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Author contact: EVSEEVA Daria (daria.evseeva@tuebingen.mpg.de)

Emergence and evolution of a novel lineage of Ralstonia solanacearum with expanded host range

EVSEEVA D. (1), PECRIX Y. (2), POUSSIER S. (4), WICKER E. (3), KUCKA M. (5), CHAN F. (5), MCCANN H. (1)

(1) Max Planck Institute for Biology, Tübingen, GERMANY; (2) CIRAD, Réunion, FRENCH SOUTHERN TERRITORIES; (3) CIRAD, Montpellier, FRANCE; (4) Université de la Réunion, Réunion, FRENCH SOUTHERN TERRITORIES; (5) Max Planck Friedrich Miescher Laboratory, Tübingen, GERMANY

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Members of the Ralstonia solanacearum species complex (RSSC) are historically known to cause brown rot of potato and Moko disease of banana in Central America. A novel lineage detected in the island of Martinique in 1999 exhibited a dramatically different host range and pathogenicity profile. This new lineage, referred to as 4NPB, can infect cucurbits Anthurium and Heliconia spp. and is more aggressive on solanaceous crops than previous variants. We sequenced 460 RSSC strains sampled across Martinique and French Guiana during the emergence of this novel lineage. Pangenome and phylogenetic analyses were performed to identify genomic changes linked with the emergence of the new lineage. This analysis reveals the 4NPB population likely emerged from a population of Moko-causing strains found in association with a tomato host on the mainland, followed by dispersal to Martinique. While 4NPB and Moko-causing strains are closely related, variation in the accessory genome includes the exchange of Type 3 secreted effectors and multiple genes with predicted catalytic activity. Recombination hotspots found between 4NPB and Moko strains include various toxin-antitoxin systems, which are potentially involved in intra-species competition and signaling. These changes may underly the altered pathogenicity and host-range profile of 4NPB, contributing to the dramatic expansion of this lineage across Martinique.

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3:00 - 3:20 - Invited speaker: Dr. Remi Peyraud (iMEAN)

3:20 – 3:40 pm - Invited speaker: Dr. Stephane Genin (Laboratoire des Interactions Plantes Micro-organismes Evironnement (LIPME), INRAE, CNRS)

Title:Addressing the nutritional virulence concept in Ralstonia solanacearum from -omics data

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1:45 - 2:05 pm - Invited speaker: Prof. Marc Valls (Universitat de Barcelona, Center for Research in Agricultural Genomics (CRAG))

Title: The Ralstonia solanacearum virulence and fitness genes

2:05 - 2:25 pm - Invited speaker: Dr. Andrea Harper (University of York)

Title: Exploiting reservoir hosts of R. solanacearum

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11:10 - 11:30 am - Invited speaker: Prof. Wei Zhong (Nanjing Agricultural University)

Title: Microbial “helpers” of Ralstonia solanacearum in plant rhizosphere

11:30 - 11:50 pm - Invited speaker: Dr. Clara Torres-Barcelo (INRAE)

Title: Unveiling phage-bacteria interactions in the light of Ralstonia solanacearum's phylogeny

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9:05 - 9:25am - Invited speaker: Dr. Alice Guidot (INRAE, CNRS)
9:25 - 09:45 am - Invited speaker: Dr. Tiffany Lowe-Power (UC Davis)
09:45 - 10:05 am - Invited speaker: Prof. Ville Friman (University of Helsinki/University of York)

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Dont forget to vote for the best ECR talk!

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Dont forget to vote for the best ECR talk!

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●  Session 1. Ralstonia solanacearum evolution
10:05 - 10:20 am - Short talks (7 min each)
- Daria Evseeva, Title: Understanding the ecology and evolution of bacterial wilt disease in the plant microbiomes

-  Dr. Florien Gorter, Title: Host range of a new Ralstonia pseudosolanacearum (phylotype I) strain detected in Dutch surface waters and bittersweet

●     Session 2. Interactions between Ralstonia solanacearum and the plant microbiome
11:50 - 12:05 pm - Short talks (7 min each)
- Dr. Virginia Ferreira, Title: Comparison of rhizospheric bacterial communities of potato genotypes with diverse defense responses against Ralstonia solanacearum

-  Dr. Rekha Gopalan Nair, Title: Contact dependent growth inhibition in Ralstonia pseudosolanacearum

●    Session 3. Plant responses and Ralstonia solanacearum virulence
2:25 - 2:40 pm - Short talks (7 min each)
- Weiqi Zhang, Title: Role of PR proteins in tomato defence against Ralstonia solanacearum

-  Dr. Sara Franco Ortega, Title: Using bacteriophages as evolutionary tools to control bacterial wilt disease: plant transcriptomic response to phage-resistant bacteria

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Postdoctoral Research Associate at University of York (UK)
sara.francoortega@york.ac.uk
@SaraFrancoOrteg

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Professor in Microbiology at University of Helsinki (Finland)
ville-petri.friman@helsinki.fi
@FrimanScience

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Ville-Petri Friman | People finder | University of Helsinki

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Lecturer in Plant Sciences at the University of York (UK)
andrea.harper@york.ac.uk
@Andrea_L_Harper

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We thank BSPP for funding this event!
https://www.bspp.org.uk/

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AGENDA

●     9 - 9:05 am Welcome

●     9:05 - 10:35 am Session 1. Ralstonia solanacearum evolution
●     10:35 - 11:10 am Coffee break

●     11:10 - 12:25 pm Session 2. Interactions between Ralstonia solanacearum and the plant microbiome
●     12:25 - 1:45pm Lunch break / Networking session (Lunch boxes provided)
●     1:45 - 3:00 pm Session 3. Plant responses and Ralstonia solanacearum virulence
●     3:00 – 3:50 pm Session 4. The era of big data – New technologies to analyse and understand bacterial wilt
disease
●     3:50 - 4:00 pm Closing session with highlights and presentation awards for ECRs
●     4:00 - 4:30 pm Coffee break / Networking session

ICPP Welcome Reception starts at 6 pm

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Title: Addressing the nutritional virulence concept in Ralstonia solanacearum from -omics data

https://www.lipme.fr/rap-membres

He is the head of the Ralstonia Adaptation and Pathogenesis team in LIPME (Laboratoire des interactions plantes – microbes – environnement) in Auzeville (France). He obtained his PhD from the Paris XI-Orsay University and follow it with a post-doctoral fellowship at the Department of Genetics at the University of Munich (Germany) investigating the growth of the filamentous fungi Ustilago maydis. He moved after that to LIPME, a laboratory which combined INRAE (Institut national de la recherche agronomique) and CNRS (Centre national de la recherche scientifique) funding. His research focuses on understanding the adaptative potential of R. solanacearum to the plant and the environment, especially the ability of this bacterium to colonize a wide range of plant species and understanding the molecular mechanisms behind bacterial wilt disease in different host plants such as Arabidopsis and tomato. His group has identified essential pathogenicity determinants such as the Type 3 protein secretion systems. His research has moved to a system-biology approach which allows the reconstruction of metabolic and virulence regulatory networks to underpin the dynamics of the pathogen during plant infection.

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Title: Systems Biology of Ralstonia solanacearum, from model strain to species complex

https://imean-biotech.com/about/

He is the CEO and co-founder of the life modelling company iMEAN (Toulouse, France). He studied biochemistry and he got his PhD in systems biology at ETH Zürich (Switzerland). After obtaining EU funding, he started modelling living organisms during his time at INRAE (Toulose, France) by using system biology approaches which lead to the funding of the startup iMEAN in partnership with Lucas Marmiesse. iMEAN focuses on producing state-of-the-art in silico models analysis and developing innovative digital services for biotechnology companies and academics. To do that, they use mathematical representations of the molecular networks at the genome level using omics data, especially transcriptomics and metabolomics.

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Title: Exploiting reservoir hosts of R. solanacearum

https://www.york.ac.uk/biology/research/plant-biology/andrea-harper/

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Title: The Ralstonia solanacearum virulence and fitness genes

https://www.cragenomica.es/staff/marc-valls
http://www.ub.edu/bactplantdiseases/members.html

He is a Professor at the Department of Genetics, Microbiology and Statistics at the University of Barcelona (Spain) and a Research leader at CRAG (Centre for Research in Agricultural Genomics, Barcelona, Spain) leading the group focused on bacterial plant disease and plant cell death. He studied biology at the University of Barcelona, and obtain his PhD at the same university. Then he did his first postdoc at the CSIC, the Spanish National Biotechnology Center in Madrid, working in molecular environmental microbiology, followed by a Marie Curie/EMBO postdoctoral fellowship at the Laboratory of Plant-Microbe interactions (LIPM) part of INRAE (Toulouse, France). In 2008, he obtained a lectureship at the University of Barcelona. His team is focused on understanding the molecular mechanisms underpinning the interaction between plant-pathogenic bacteria in order to obtain sustainable strategies to combat plant pathogens in the field. His group is divided into two areas: understanding genetic determinants of R. solanacearum to find novel targets to control the disease, as well as understating the plant's response to the bacterial invasion, identifying candidate genes, metabolites and mechanisms behind the plant's defence.

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Title: Unveiling phage-bacteria interactions in the light of Ralstonia solanacearum's phylogeny

https://www6.paca.inrae.fr/pathologie_vegetale_eng/Contact-us/Mistral-team/TORRES-BARCELO-Clara

She a researcher at the Plant Pathology Unit at INRAE (Institut national de la recherche agronomique) in Avignon, France. She completed her PhD at the Institute of Molecular and Cell Plant Biology (Polytechnic University of València – Spanish Research Council) in Valencia (Spain) studying molecular compensatory evolution of plant viruses. In 2010, she moved to the Department of Zoology at the University of Oxford (UK) holding a postdoctoral fellowship. During this time, she studied the evolutionary aspects of stress responses of the major human and animal pathogen Pseudomonas aeruginosa when exposed to antibiotics. In 2013, she moved to ISEM (Institute of Evolutionary Sciences of Montpellier, France) where she continued her research on P. aeruginosa evolution of resistance when exposed to antibiotics and bacteriophages at the same time. She held a one-year lectureship at the University of Montpellier (France) and another post-doctoral contract at the University of Reunion Island (France). In 2019 she moved to INRAE. Her research focuses on the interaction between bacteriophages and bacteria in agricultural environments, especially focusing on plant pathogenic bacteria such as R. solanacearum, Erwinia amylovora and Pseudomonas syringae.

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Title: Microbial “helpers” of Ralstonia solanacearum in plant rhizosphere

https://english.njau.edu.cn/mainm.htm

He is a Professor at Nanjing Agricultural University (China). He studied Agricultural resources and environment bachelor at Nanjing Agricultural University followed by a PhD in Plant nutrition at the same university. His group is focused on understanding the rhizosphere microbiome interactions and their role in plant health, especially on identifying the microbiomes that distinguish healthy and diseased plants, their function and how they interact with each other. He is also interested in the possibilities of managing the soil microbiome to design novel bio-products to improve plant health.

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Title: Phage resistance-virulence trade-offs in Ralstonia solanacearum

https://www.helsinki.fi/en/about-us/people/people-finder/ville-petri-friman-9089357

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Ville-Petri Friman | People finder | University of Helsinki

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Title: Understanding Ralstonia's adaptations to their lifecycle through the lens of barcoded genetic screens

https://plantpathology.ucdavis.edu/people/tiffany-lowe-power

She is an Assistant Professor at the University of California, Daivs (California, USA). She obtained her Bachelor degree in Applied Biology at the Georgia Institute of Technology and her PhD in the Department of Plant Pathology University of Wisconsin–Madison where she studied metabolic changes of R. solanacearum during plant pathogenesis, especially how the degradation of plant defences protect R. solanacearum from toxicity and how the production of metabolites accelerate disease. She followed her PhD with a postdoc at the University of California, Berkeley studying the molecular microbial ecology of plant-associated bacteria. Her team is formed by plant pathologists and microbiologists and focused on the fitness adaptations of the bacteria when colonizing the plant and causing disease. Her team uses R. solanacearum as a model to study molecular microbial ecology, host specificity and physiology to understand how the host shapes bacterial behaviour and vice versa, how xylem pathogens also modify the host environment.

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Title: Multihost experimental evolution of Ralstonia solanacearum unveils genetic and epigenetic bases involved in adaptation to plants

https://www.lipme.fr/rap-membres

She is a researcher at LIPME (Laboratoire des interactions plantes – microbes – environnement) in Auzeville (France). She studied Microbial Ecology at Lyon University followed by her PhD at the same university studying ectomycorrhizal fungus Hebeloma cylindrosporum populations. In 2004 she started her first post-doctoral fellowship at Uppsala University (Sweden) studying the distribution and persistence of populations of the postfire wood decay fungi Daldinia loculata and ectomycorrhizal Lactarius mammosus. After 4 years, she moved to CIRAD (French Agricultural Research Centre for International Development) at La Réunion (France) where she started working with R. solanacearum by doing comparative genomic analysis of tomato strains within the R. solanacearum species complex. She studied horizontal gene transfer and specific strain-specific genes that could be used for phylotype identification. She moved to Stephane Genin’s Ralstonia Adaptation and Pathogenesis team at LIPME in 2008 continuing her work in R. solanacearum and focusing on experimental evolution experiments, especially on how the bacteria adapts to the host. In 2019, she obtained the French Habilitation à Diriger les Recherches to manage her own research.