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The brain sends a nerve impulse through the motor unit to a Neuromuscular Junction.
Nerve impulse causes voltage gated calcium ion channels to open which allow calcium ions to enter the neuron.  This increase in Calcium stimulates the release of the neurotransmitter, acetylcholine, which is released into the cleft. ACh binds to a ACh receptor sight and activates voltage gated sodium channel  which pumps Na+ in and K+ out. More Na+ is pumped into the muscle cell than K+ is pumped out causing the cell interior to become less negative or depolarized.