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microbial biotransformation https://app.nearpod.com/?pin=49F7F2FD9D63B5A026146B5A0487A743-1

https://edpuzzle.com/assignments/6188cd67bccbe841996ad9da/watch

https://edpuzzle.com/assignments/61886b208eab3f419c5e4f62/watch

https://edpuzzle.com/assignments/6188684ea45985418c0abae1/watch

https://edpuzzle.com/assignments/6170e8aaeedf3d416e9c8013/watch

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No mobile devices in your classroom? No worries! You can enjoy Nearpod from any web browser :) Create, engage, and assess your students in every lesson!

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MUHAMMAD RUSYAIDI BIN ROSLI | A18SC0422

NOR HAIZAADIELA BINTI ABDUL RAHMAN | A18SC0429

NUR ATIQAH ABD MUTALIB | A18SC0441

YONG JIA YI | A18SC0496

e) With the depletion of fossil fuels, researchers are searching for an alternative energy source that is environmentally friendly. Ideally from microorganisms that are able to produce hydrogen.
i) The researchers would be bioprospecting the microbial community in producing alternative fuel. Explain one method you would choose and why. (5 marks)

Bioprospecting is the exploration of biodiversity for new sources of social and commercial values.
The advent of metabolic engineering and the increase in the number of whole-genome sequenced organisms has contributed to improvement in the manipulation of microbial metabolic pathways and the production of numerous essential chemicals for the production of biofuel.

ii) Propose the type of location, the type of microorganisms, the species or genera, the metabolism and mechanism in producing renewable energy, when is the product produce (primary or secondary). (15 marks)

Type of location: Sewage sludge
Type of microorganisms: Bacteria
Species: Clostridium butyricum

Clostridia are Gram-positive, anaerobic or obligate anaerobic bacteria that are among a metabolically diverse group .Clostridia perform diverse metabolic functions, including the conversion of starch, protein, and purines into organic acids (i.e., acetic, butyric, and caproic acids), alcohols, CO2, and hydrogen. Because of their broad and flexible metabolic capabilities, Clostridium species are included in many microbial co-culture ecosystems, which we will refer to in this review as “Clostridium co-culture systems.”
Typically, Clostridium co-culture systems are used to produce biofuels such as hydrogen and CH4, solvents, and organic acids. Because cellulosic materials are commonly found in nature , the specific metabolic capacities of cellulolytic strains and producers in co-culture systems have attracted significant attention and offered many long-term prospects for development.
The co-culture of C. cellulovorans and C. acetobutylicum achieved a 2- to 3-fold improvement in hydrogen production in comparison with C. acetobutylicum mono-cultures from lignocellulosic substrates.

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MUHAMMAD ASHYRAF BIN AZREEN (A18SC0421)

NUR SYAMIERA BINTI SANI (A18SC0452)

NUR AFIQAH BINTI KAMAL (A18SC0459)

NURUL NAZIHAH BINTI KASIM (A18SC0463)

g) Removal of sulfide minerals in soil sample. The soil is taken from a mining environment where organic nutrients are extremely low.

i) Describe and explain the metabolism of this microorganism (8 marks)

Removal of sulfide minerals is defined as oxidation of sulfide by the microbes. The metabolism of this microorganism is chemolithoautotrophic. This microbe obtains energy from chemical compounds and since the organic nutrients are extremely low, it can derive the energy from the inorganic compound which in this case is sulfide by oxidation of sulfide to sulfate. The oxidation of sulfide can occur in two ways which are aerobically and anaerobically. Aerobically, the oxidation can occur in substrate-level phosphorylation depending on AMP (transfer phosphate group to ADP to produce ATP) or in oxidative phosphorylation (ETC) where the sulfide acts as electron donor and oxygen as electron acceptor to produce ATP. Anaerobically, sulfide will oxidize to sulfate by using nitrate as terminal electron acceptor which then reduces to dinitrogen in ETC with the help of sulfite oxidase enzyme. Since organic nutrients are low, this microbe can only obtain carbon source from inorganic carbon, which is through carbon dioxide fixation (calvin cycle) using the energy source from the oxidation of sulfide. It can also use the energy of the reducing power from reverse electron flow using sulfur as a higher redox potential that can reduce NAD+ to NADH. NADH is then used in the calvin cycle for carbon source. Thus, it is considered as autotroph.  Example of bacteria is thiobacillus thiooxidans.

ii) What is the primary and secondary product of the microorganism? (2 marks)

NADH as primary product while ATP as secondary product.

iii) List the application use for this type of metabolism. (2 marks)

Bioleaching or biomining

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Muhammad Aidil Izzuddin Bin Sudarman (A18SC0417)

Muthiah Nuri Rahmani (A18SC0504)

Siti Fatihah Hidayatul Binti Halim (A18SC0475)

Fatin Natasha Binti Yusof (A18SC0392)

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NIK AHMAD NA'IM BIN JOHN ISMAIL (A18SC0425)

MOHAMAD KHAIRUL AZIZI BIN KHASIM (A18SC0414)

NUR AIDA FARZANA BINTI FADZLI (A18SC0434)

NUR SYAKIRA BINTI MOHD HAMIDI (A18SC0451)

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MASTURAH BINTI SHALEHUDDIN (A18SC0410)

ZULHISHAM BIN ZULZAHRIN (A18SC0498)

ANAS AZIZI BIN MOHD ASRI (A18SC0377)

SITI NOORASHIKIN BIN HARIZAN (A18SC0477)

f) Discuss the microbial community and nutrient requirement and possible metabolism that are present in an acid mine drainage.

Acid mine drainage is a formation and movement of highly acidic (sulphuric acid) water rich in heavy metals. This acidic water forms through the chemical reaction of surface water (rainwater, snowmelt, pond water) and shallow subsurface water with rocks that contain sulfur-bearing minerals, resulting in sulfuric acid.

As such, microbes that are able to suvive in acid mine drainage are microbes that uses chemicals as an energy source (chemo-), with its chemical being some type of inorganic chemicals (-litho-) and also uses inorganic carbon, like CO2 (-auto). Hence,  microbial community that resides on the acid mine drainage are acidophilic chemolithoautotrophic microbes

In acid mine drainage :

- microbial community: acidophile microbes

- nutrient requirement: Sulphur

- possible metabolism that are present in an acid mine drainage – chemolithoautotrophy

- Sulfate is essentially chemically inert, nonvolatile and not toxic and hence sulfate emissions may be viewed as benign to the environment. However, it is recognized that high sulfate concentrations can negatively influence the natural sulfur cycle.

- As a treatment option, Sulphate-Reducing Bacteria (SRB) use sulfate  as a terminal electron acceptor while selected organic compounds can donate electrons. This treatment will neutralize the acidity of the drain and also percipitate the heavy metals that present there

- Example, the anaerobes of the genus Desulfovibrio are capable of reducing SO to H2S. Hydrogen sulphide is thus promote alkalinity and likely to accumulate in waterlogged soils.  Hydrogen sulphide reacts with lead, iron and zinc to form insoluble precipitates. Thus, in areas where H2S is prevalent, iron metal will not be available in the soluble form which is required for all organisms.

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MUHAMMAD AIDIL WAFI BIN ROSLI | A18SC0418

NUR ADLINA BINTI NAZRI | A18SC0433

NUR AINA SOFIA BINTI ZUBIR | A18SC0435

SITI ZULAIKHA BINTI SYAMSUL BAHRI | A18SC0480

Chemolithotrophy questions and answers:

1) For what two purposes are inorganic compounds used by chemolithotrophs?

Inorganic compounds used by chemolithotrophs are for their energy and electron needs.

2) Why are the sulphur-oxidizing lithotrophs typically acidophiles?

Sulphur oxidation reactions products present a proton production which results in acidification of the environment or medium thus lowering the pH. Acidophiles are the only microorganism that could live under an acidic environment and managed to oxidize sulphur compounds to produce sulphuric acid, therefore, it results in acidophiles as sulphur-oxidizing lithotrophs.

3) What is the function of rusticyanin and where is it found in the cell?

Rusticyanin is an electron acceptor from Fe2+. It is a periplasmic copper-containing protein. It could be found in periplasmic of the cells.

4) What is the inorganic electron donor for Nitrosomonas? For Nitrobacter?

Inorganic electron donor of Nitrosomonas is ammonia while for Nitrobacter is nitrite.

5) What do nitrifying bacteria use as a carbon source?

Nitrifying bacteria use carbon dioxide as their carbon source because they are chemoautotrophs.

6) What is the anammox reaction and how does it differ from aerobic nitrification?

Anammox is a reaction under anoxic conditions which oxidizes ammonium to a gas known as dinitrogen using a nitrite as electron acceptor.

Tutorial question and answers:

a) With every breath you take, thank the ocean. Based on this statement, discuss the diversity of microorganisms and its metabolism that are responsible for giving us the means to breathe. (10 marks).

Breathing is a process of taking oxygen and releasing carbon dioxides.

Oxygen is needed to undergo the cell respiration process to generate energy inside our body.

Oxygen is mostly created under a photosynthesis process from photosynthetic organism.

The statement shows photosynthetic organisms not only present on the land but also in the ocean because 71% of Earth is covered by ocean and only 29% covered by land.

Several photosynthetic organisms in the ocean that are responsible for making oxygen known as marine photosynthesizer such as phytoplankton and seaweed.

Both marine photosynthesizers are photolithoautotrophs where their energy source is from the sunlight, the electron source is from inorganic compound known as water and their carbon source is carbon dioxides.

Photolithoautotrophs contain photosynthetic pigments that will trapped the light energy from the sunlight.

This light energy will be converted into chemical energy through photophosphorylation to transfer electrons from oxidizing water molecules.

The chemical reaction as below:

2H2O  → 4H+ + O2 + 4e

For every 2 molecules of water, it will be oxidized through phosphorylation into four protons, four electrons and oxygen.

The four electrons would be transferred further making water molecules known as electron donor while oxygen will be released into the environment.

It was known that half of the oxygen released into the environment is from the ocean.

Therefore, this statement shows that we have to appreciate the ocean by acknowledging the importance of it for our lives.

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1. Type of location

In soils

2. Type of microorganisms and Species/ genera

Archaea
-Euryarchaeota -Crenarchaeota

Bacteria
-Aquificae
-Actinobacteria
-Chloroflexi Proteobacteria
-Chlorobi,
-Firmicutes -Thermodesulfobacteria

3. Metabolism & Mechanism

The first step of soil carbon sequestration is the process of photosynthesis. The sun will provide energy to the plants to perform photosynthesis. Next, the plants will absorb the carbon from the atmosphere to build its roots, stems and leaves. It only returns some of the carbon to the atmosphere through process of respiration. The roots of the plants will release some of the carbon to the soil for the microorganisms to gain their food. When the microbes die, the carbon inside their bodies will dissolve with the mineral and increase the microbial carbon cycles through the soil. Thus, it will increase the soil carbon stocks.

CARBON SEQUESTRATION IN SOILS. (n.d.). https://www.esa.org/esa/wp-content/uploads/2012/12/carbonsequestrationinsoils.pdf

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MUHAMMAD ASHYRAF BIN AZREEN (A18SC0421)

NUR SYAMIERA BINTI SANI (A18SC0452)

NUR AFIQAH BINTI KAMAL (A18SC0459)

NURUL NAZIHAH BINTI KASIM (A18SC0463)

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KHAYRIN ILYANA BINTI MOHD LOTFI
(A18SC0508)
ZAWANI NADZIRAH BINTI ZAMANI
(A18SC0497)
MOHAMAD FIRZAN BIN MOHD NOOR
(A18SC0413)
THIWANTINI ASOGAN
(A18SC0490)

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MUHAMMAD RUSYAIDI BIN ROSLI | A18SC0422

NOR HAIZAADIELA BINTI ABDUL RAHMAN | A18SC0429

NUR ATIQAH ABD MUTALIB | A18SC0441

YONG JIA YI | A18SC0496

1) Secondary metabolite
Streptomycin.

2) How does the secondary metabolite been produced?

Fermentation

Sterilize all the above contents and fed into the stirred tank fermentor. Then, transfer the bacterial inoculum of about 4-5% to the medium. Maintain a temperature between the range of 25-30 degrees Celsius and pH between 7.6-8 inside the fermentor. The production of streptomycin requires continuous fermentation, and the growth of S.griseus requires continuous aeration and agitation. The fermentation process takes 4-10 days. The fermentation process involves three consecutive phases.

First phase

During the first phase, the S.griseus grows by utilizing soya meal and releases ammonia through proteolytic activity. The pH rises during this phase. S.griseus slowly utilizes the carbon source present in the soy meal and the glucose and thereby producing streptomycin at a slower rate.

Second phase

After the utilization of soy meal and glucose, the ammonia is secreted, and the streptomycin is synthesized rapidly. In this phase, the incubation period lasts for 24 hours to 6-7 days. The pH during this phase remains constant between 7.6 and 8. There is little or no production of mycelium.

Third phase

It is the final phase of fermentation, where the concentration of glucose depletes from the medium that results in cessation of streptomycin production, and the bacterial cells begin to lyse. As a result, the medium’s pH increases by the deposition of ammonia after the cell lysis. Before cell lysis, harvest the fermentative medium for the process of recovery and purification.

3) Relate primary and secondary metabolism of the secondary  metabolite

Antibiotics are products of secondary metabolism from streptomycin that inhibit the growth of other microorganisms even at low concentrations. To be able to obtain secondary metabolites, metabolic pathway reaction methods are conducted using multienzyme complexes or an individual enzyme. It is generally believed that secondary metabolism is derived from primary metabolism by gene duplication and possibly by allelic divergence. The primary mechanism of action is inhibition of translation by binding to the ribosome.

4) Which microbes is it from and what other microbes produce this secondary metabolite?

Original microbe producing streptomycin: Streptomyces griseus

Other: Streptomyces glaucescens

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Muhammad Aidil Izzuddin Bin Sudarman (A18SC0417)

Muthiah Nuri Rahmani (A180504)

Siti Fatihah Hidayatul Binti Halim (A18SC0475)

Fatin Natasha Binti Yusof (A18SC0392)

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NIK AHMAD NA'IM BIN JOHN ISMAIL (A18SC0425)

MOHAMAD KHAIRUL AZIZI BIN KHASIM (A18SC0414)

NUR AIDA FARZANA BINTI FADZLI (A18SC0434)

NUR SYAKIRA BINTI MOHD HAMIDI (A18SC0451)

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MUHAMMAD AIDIL WAFI BIN ROSLI | A18SC0418

NUR ADLINA BINTI NAZRI | A18SC0433

NUR AINA SOFIA BINTI ZUBIR | A18SC0435

SITI ZULAIKHA BINTI SYAMSUL BAHRI | A18SC0480

Phototrophic questions and answers:

1. What is the difference between oxygenic and anoxygenic phototroph?

For CO2 reduction, oxygenic phototroph use H2O as electron donor while anoxygenic phototrophs use reduced sulfur (H2S) or molecular hydrogen (H2) as electron donor.

For oxygenic phototroph produce oxygen (O2) while anoxygenic phototroph does not produce oxygen

2. How are photoautotrophs and photoheterotrophs similar and how do they differ?

Similarity: both use light energy and carry out photosynthesis process

Differences: photoautotrophs use CO2 as carbon source while photoheterotrophs use organic carbon as carbon source

3. Group the photosynthetic bacteria according to its trophy and oxygenic/anoxygenic phototrophs.

Photoautotrophs: oxygenic ( cyanobacteria, prochlorophyta), anoxygenic (green sulfur bacteria and purple sulfur bacteria)

Photoheterotrophs: anoxygenic (purple non-sulfur bacteria, green non-sulfur bacteria and heliobacteria)

Secondary Metabolites questions and answers:

1. Secondary metabolite

Penicillin G is an important antibiotic under a class of β-lactam.

2. How is the secondary metabolite produced?

Penicillin is produced by strains of Penicillium notatum or Penicillium chrysogenum when inoculated into a suitable nutrient medium.

The biosynthesis of benzyl penicillin occurs via the condensation of its precursors, phenyl acetic acid, L-cysteine, and L-valine.

The rate-limiting reaction is the synthesis of the side-chain precursor, and thus phenyl acetate is usually added to the fermentation medium.

Acylation of the β-lactam-thiazolidine ring appears to be one of the final steps in biosynthesis

3. Relate primary and secondary metabolism of the secondary  metabolite.

Primary metabolism of penicillin G is glycolysis and the Krebs cycle  that are for growth and multiplication of fungi.

Glycolysis in cytoplasm is responsibles in producing pyruvate which then would be preceded in the Krebs cycle in mitochondria process for producing α-ketoglutarate.

Pyruvate from glycolysis would also be oxidized by NAD+ into acetyl-CoA in cytoplasm while α-ketoglutarate would leave the mitochondria to undergo secondary metabolism that are not involved in growth and multiplication of fungi which is penicillin biosynthesis pathway.

Acetyl-CoA and α-ketoglutarate would interact between each other forming α-aminoadipate.

α-aminoadipate would interact with L-cysteine forming L-α-aminoadipylcysteine.

This compound would then undergo chemical interaction with L-valine to form ACV-tripeptide.

This chemical complex is responsibles in producing Penicillin G for secondary metabolism

4. Which microbes is it from and what other microbes produce this secondary metabolite?

Penicillin is produced by fungi, especially of Penicillium mold. In regards to this activity, penicillin is specifically produced by fungus Penicillium chrysogenum. Although it is commonly produced by the aforementioned fungus, P. rubens and P. notatum are also some of the known producers of Penicillin.

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MASTURAH BINTI SHALEHUDDIN (A18SC0410)

ZULHISHAM BIN ZULZAHRIN (A18SC0498)

ANAS AZIZI BIN MOHD ASRI (A18SC0377)

SITI NOORASHIKIN BIN HARIZAN (A18SC0477)

Questions and Answers

1. Secondary metabolite,

Polyhydroxyalkanoate (PHA)

2. How does the secondary metabolite been produced?

Polyhydroxyalkanoates are a class of natural (not synthetic) polyesters that are derived from bacterial fermentation of sugar or lipids. Basically, microorganisms synthesize polyesters in nutrient-deficient conditions, and these PHAs can then be harvested.

3) Relate primary and secondary metabolism of the secondary  metabolite

Biosynthesis of PHA is usually caused by lack of macro elements like phosphorus, nitrogen, trace elements and oxygen but excess in carbons. the primary metabolite products that made up PHA are all carbon-rich molecules

4) Which microbes is it from and what other microbes produce this secondary metabolite?

Cupriavidus necator, recombinant Bascillus subtilis

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Name: YONG JIA YI
Lives : Petaling Jaya ,Selangor
Likes : Travel , cafe hop, photography , nature.
Ambitious: Get rich to enjoy all the funs.

# data[].attributes.body
Name: Nurul Afiqah Binti Kamal
Lives: Limbang,Sarawak
Likes: Music,Traveling,Novels
Ambition: Living in health, good and stable life.

# data[].attributes.body
Name : Mohamad Khairul Azizi bin Khasim
Lives : Simpang Renggam, Johor
Likes : Games, Movies, Travel
Ambition : Time and Financial freedom

# data[].attributes.body
Name : Thiwanthini Asogan
Lives : Skudai, Johor
Likes : foods hunting, animals, travelling, planting, flowers, potraits, colouring, singing, dancing.
Ambition : Achieve my future goals, become scientists, travels worldwide, and lives my happy life. ;)

# data[].attributes.body
Name: Muthiah Nuri Rahmani
Lives: Bandung, West Java, Indonesia
Likes: Sunset, rainy season, taking pictures with camera, crime movies and dramas, cooking and baking.
Ambition: To become a dietitian, travel around the world, also live a less waste life

# data[].attributes.body
Name: Mohamad Firzan Bin Mohd Noor
Lives: Puncak Alam, Selangor
Likes: Anime, games
Ambition: Able to smile even when I'm hurt

# data[].attributes.body
Name : Nik Ahmad Na'im bin John Ismail
Lives : Kluang, Johor
Likes: Foods, Read, Action movies, Historic documentaries, Musics, Stories, Gaming, Drawings, Photo Editing, Travelling.
Ambition : Living my dream

# data[].attributes.body
I am your course lecturer for SMBT 4183.
Name : Dr Siti Halimah Hasmoni
Lives : Johor Bahru
Likes : Adele, Beaches, cafe, crime docu series and Coffee
Ambition : reading all the books that i bought and Living my dream

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Name: Nur Aina Sofia binti Zubir
Lives: Rawang, Selangor
Likes: Coffee, Anime, Kdrama, Cats, Sing
Ambition: Able to finish reading even just a thin book cuz I'm not a reader hehe

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Hi, I'm wafi or also go by the name of thewafism.

# data[].attributes.subject
Hi, I'm wafi or also go by the name of thewafism.

# data[].attributes.body
Name: Muhammad Aidil Wafi bin Rosli
Lives: Banting, Selangor.
Likes: Adele too! The Sims, City Skylines, nature, sitcom, anime, cartoon, romcom, any talent competition show, I recently grow fond for yellow colour, listening to various type of music (pop, rnb, ballad, opera, rock, metal, jazz and any kind of songs), english (but aint pro), shopping
Ambition: so many till i dont know how to tell, but some of them are, playing all instruments, publish book, have a big house, become richer, have a good life in a great comfortable pace, be confident and proud of myself every single day without thinking other peoples opinion.

# data[].attributes.body
Name: Nur Atiqah Abdul Mutalib
Lives: Batu Pahat, Johor
Likes: Cats, foods, movies, badminton
Ambition: To be a better me in future

# data[].attributes.body
Name: Nur Syamiera binti Sani
Lives: Batu Pahat, Johor
Likes: Anime, Nature, Sports, Cats, Piano, Musics, Graphics, Videos, Animation and being myself
Ambition: Make my parents proud, live my life to the fullest and  get married 😂 jkjk

# data[].attributes.body
Name : Nor Haizaadiela bt Abdul Rahman
Lives : Kemaman, Terengganu
Likes : Desserts, travelling, gather with friends, beaches too and now I am trying to be a book lover.
Ambition : Being someone that benefit to many people, futfill my purpose of life and get to go to everywhere I want, aamiin !

# data[].attributes.body
Name: Zulhisham bin Zulzahrin
Lives: Sungai Buloh, Selangor
Likes: sticker deco, eating, movies, sending tiktoks to my friends, spending money and gossip
Ambition: To live a busy researcher life who also travels a lot

# data[].attributes.body
Name : Nurul Nazihah Kasim
Lives : Bera, Pahang
Likes : Having online friends from other country, listening to music, living my own world (alone)
Ambition : Travel around the world with my own money, loving myself better

# data[].attributes.body
Name: Fatin Natasha Binti Yusof

Lives: Johor Bahru

Likes: Music and lyrics (All genre but prefer baroque, Lana Del Ray), Poetry, Deep conversation, Voluntary works and Sciences

Ambition: To be happy :')

# data[].attributes.body
Name: Nur Syakira binti Mohd Hamidi
Lives: Segamat, Johor.
Likes: reading novels, cooking, watch movies, cartoon, anime, I love blue colour
Ambition: I want to  be a teacher, have my own house and my own car, be a good daughter to my parents and always be good to others

# data[].attributes.body
Name: Masturah Binti Shalehudin

Lives: Sungai Buloh, Selangor

Likes: +×+, webtoon, kdrama, ice cream, FamilyMart, Mcd, sleep, etc

Ambition: to live a peaceful life 🥴

# data[].attributes.body
Name: Nur Aida Farzana binti Fadzli

Lives: Seberang Jaya, Pulau Pinang.

Likes: Art, Photographing, Anime, Talking about life, Nature.

Ambition: Be someone that can inspired others.

# data[].attributes.body
Name : Zawani Nadzirah binti Zamani

Lives : Seri Kembangan, Selangor

Likes : EXO (KPOP group), Cats, Baking, Swimming, Playing games, Reading novel and History (a boring subject for some people but meh >.> I like it)

Ambition :
- Make my beloved parents and family proud of me
- Reading all the books that I bought too
- Be confident since I am still too shy :')
- Open my own bakery in the future
- Lastly, secure a job and become rich. So, my parents won't need to work anymore and just rest at home.

# data[].attributes.body
Name: Muhammad Rusyaidi Bin Rosli
Lives: Bayan Lepas, Penang.
Likes: Five letter words, games. G for gratification, A for astonishing, M for marvellous, E for exciting and S for satisfying. R+B=Purple including black and white.
Ambition: To finish this game called My Life.

# data[].attributes.body
Name: Siti Zulaikha binti Syamsul Bahri
Lives: Skudai, Johor
Likes: Imagine Dragons, Bruno Mars and The Weeknd. Cooking, reading and watching any good dramas, k-drama and anime. Learning about skincare is for sure.
Ambition: To become an aesthetician and owning an aesthetic clinic or have a skincare line or pursuing academic field till I got my PhD or just do everything I said before while enjoying a good life of mine:)

# data[].attributes.body
Name : Anas Azizi bin Mohd Asri
Lives : Kedah Darul Aman
Likes : Martial art, social media, novels
Ambition : Scientist/Nutritionist

# data[].attributes.body
Name: Siti Noorashikin bin Harizan

Lives: Sungai Buloh, Selangor

Likes: Nature, traveling, and doing stuff outdoors

My goal in life is to live a healthy life in a unique manner, never lose my self-respect, and always be self-sufficient also prove myself in my chosen field and be on top at all times.