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SLS measures the amplitude of scattering, regardless of its fluctuations.

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Difference : Dynamic light scattering monitors the fluctuations of the scattered photons over very short time intervals from the sample

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Difference : Static light scattering measures the average scattered intensity of a population of particles in solution by integrating the scattered signal over a period of time

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monitors the fluctuations of the scattered photons over very short time intervals from the sample

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measures the average scattered intensity of a population of particles in solution by integrating the scattered signal over a period of time

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Theory - DLS is based on the extraction of spectral information derived from time-dependent fluctuations of the light scattered from a spatially limited volume within the sample. Specifically, when a suspension of particles is hit by a monochromatic coherent beam of light, generated scattered light waves spread out in all directions. Scattered waves interference in the far field region generates a net scattered light intensity. Due to the random motion of the suspended particles within the sample, the interference can be stochastically either constructive or destructive, hence resulting in a stochastic light intensity signal

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Theory
dynamic light scattering is a technique that use to determine the size distribution profile of small particle in suspension.

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Application: measure the oscillation of macromolecule (brownian motion)

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The dynamic light scattering (DLS) technique is applied to the dust component of a complex (dusty) plasma, revealing a Gaussian intensity autocorrelation function for scattering angles between 4° and 175°.

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differences:
DLS-
- record of the fluctuations in scattering intensity
-determine hydrodynamic radius

SLS
-record the amount of light scattered by a sample
-determine sample properties like molecular weighty like molecular weigt and second virial coefficient by using intensity of light at different concentration

SLS-

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Theory
-to investigate the size of various object

Application
-measure scattered light angle and intensity
-transform that scattering data into a particle size distribution

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DLS uses the recording of the fluctuations in scattering intensity over time in order to characterise motion within the sample. By quantifying these fluctuations, through either correlation or spectral analysis, diffusion coefficients can be calculated which in turn can be used to determine hydrodynamic radius.

In short, DLS measures how scattering changes over time, regardless of the amplitude (amplitude only matters for instrumentation optimisation)

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Applicatipn of static
1. Determination of the weight average molecular weight of a macromolecule such as polymer or a protein.
2. measurement of the radius of the gyration or the form and structure factor.

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Theory- Static light scattering (SLS) is a technique to measure absolute molecular weight using the relationship between the intensity of light scattered by a molecule and its molecular weight and size, as described by the Rayleigh theory.

Application-   SLS is the determination of M w, which is the weighted average molecular weight of a macromolecule such as, for example, a polymer or a protein. Other popular applications of SLS include the measurement of the radius of gyration R gor the form and structure factor.

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Static light scattering involves recording the amount of light scattered by a sample, either at a range of angles and/or for different sample concentrations.

Recording the intensity of light scattered at different angles allows theoretical models to be used to determine the size, shape or structure of the scattering material.

Some SLS measurements use the intensity of light scattered at different concentrations of the sample in order to determine sample properties like molecular weight and second virial coefficient.

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Application: able to determine the way of macro molecules

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Dynamic Light Scattering (DLS) refers to an optical technique used for analyzing dynamic properties and size distribution of a broad variety of physical, chemical and biological systems composed of several suspended constituents [1]. These can be colloidal particles, macromolecules, bubbles or droplets.

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Dynamic light scattering is a technique to determine the size distribution profile of small particle in suspension.

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Theory:optical technique that measure the intensity of the scattered light in dependence
Application: to determine Mw, which is the weighted average molecular weight of macromolecule such as polymer or a protein
Difference: Measures the average scattered in solution by integrating the scattred signal over a period of time. Used to determine particle size,and if the system is appropriately calibrated, molecular weight of the particles.
GRoup 5

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Differences: the light is constantly changing

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Theory- non- invasive well establish technique to measure the size and size the distribution of molecules and particles typically in the submicron region.

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GP 7
is a technique in physics that can be used to determine the size distribution profile of small particles in suspension or polymer in solution. DLS application uses a light source such as a laser, which is shone onto particles that are in Brownian motion in solution, the light that hits the particles is scattered in all direction, which is referred to as Rayleigh scattering. The fact that the particles are undergoing Brownian motion means the light is scattered at different intensities. These intensity fluctuations can then be analysed to provide information about the size and size distribution of particles..

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Differences: the light is stay the same

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Monitor the fluctuation of the scattered photon over very short time interval from the sample (involve indication of the Brownian Movement). The purpose is to determine particle size.

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Theory-  its a technique to measure absolute molecular weight using the relationship between intensity of light scatter by a molecule and its mecular weight and size.

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Measure the average scattered intensity of a populatiom of particle in a solution by integrating the scattered signal over a period of time. The purpose is to determine appropriately calibrated molecular weight of the particle.

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A method for determining structure by measuring the total or time-average scattering intensity of scattered visible light as a function of angle. Application of static light scattering is the determintion of molecular weight which is the weighted average molecular weight of a macromolecule such as for example a polymer or a protein. (G3)

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A method for determining structure by measuring the total or time-average scattering intensity of scattered visible light as a function of angle. Application of static light scattering is the determintion of molecular weight which is the weighted average molecular weight of a macromolecule such as for example a polymer or a protein. (G3)