# data[].attributes.headline
Transfer "transformation solution" into two tubes

# data[].attributes.subject
Transfer "transformation solution" into two tubes

# data[].attributes.body
The solution helps prepare the cells for transformation by making them able to take up plasmid DNA

# data[].attributes.body
Increases the cell membrane permeability to the plasmid DNA

# data[].attributes.headline
Suspend bacterial colonies in "transformation solution"

# data[].attributes.subject
Suspend bacterial colonies in "transformation solution"

# data[].attributes.body
Gets the cells ready to take up plasmid DNA

# data[].attributes.headline
Add pGLO plasmid DNA to the "+pGLO" tube

# data[].attributes.subject
Add pGLO plasmid DNA to the "+pGLO" tube

# data[].attributes.body
Introduces the plasmid to the cells

# data[].attributes.body
Helps plasmid DNA bind to cell membranes

# data[].attributes.headline
Heat-shock and then place is back on ice

# data[].attributes.subject
Heat-shock and then place is back on ice

# data[].attributes.body
The heat opens the pores of the cells and allows the plasmid to enter, and then the cooling closes the pores, preventing it from escaping the cell

# data[].attributes.headline
Add LB broth and incubate at room temperature

# data[].attributes.subject
Add LB broth and incubate at room temperature

# data[].attributes.body
Allows the cells to recover and express the antibiotic resistance gene

# data[].attributes.body
Allows bacterial colonies to grow and express the plasmid

# data[].attributes.body
Checks for GFP expression in the transformed colonies

# data[].attributes.headline
Inoculate 2 tubes, one labeled "+" and the other "-"

# data[].attributes.subject
Inoculate 2 tubes, one labeled "+" and the other "-"

# data[].attributes.body
Prepares cultures for protein production

# data[].attributes.body
Ensures even distribution of bacteria in the tubes

# data[].attributes.headline
Incubate for 2 days, then at room temperature

# data[].attributes.subject
Incubate for 2 days, then at room temperature

# data[].attributes.body
Promotes bacterial growth and GFP production

# data[].attributes.headline
Transfer 2mL of culture into a microtube and centrifuge

# data[].attributes.subject
Transfer 2mL of culture into a microtube and centrifuge

# data[].attributes.body
Pellets the bacterial cells

# data[].attributes.headline
Pour out supernatant, leaving only the pellet

# data[].attributes.subject
Pour out supernatant, leaving only the pellet

# data[].attributes.body
Isolates the bacterial cells for lysis

# data[].attributes.headline
Add the solution to the tube and resuspend the pellet

# data[].attributes.subject
Add the solution to the tube and resuspend the pellet

# data[].attributes.body
Prepares for bacterial lysis

# data[].attributes.headline
Add a drop of lysozyme and mix gently, then freeze

# data[].attributes.subject
Add a drop of lysozyme and mix gently, then freeze

# data[].attributes.body
Begins lysing the bacterial cells and preserves sample for the next experiment section

# data[].attributes.body
Separates the lysate from the cell debris

# data[].attributes.body
Sets up for protein purification

# data[].attributes.headline
Add aliquots of the supernatant into the top of the column

# data[].attributes.subject
Add aliquots of the supernatant into the top of the column

# data[].attributes.body
Loads the lysate onto the column for separation

# data[].attributes.body
Helps the binding of proteins to the column

# data[].attributes.headline
Transfer binding solution to another microtube

# data[].attributes.subject
Transfer binding solution to another microtube

# data[].attributes.body
Prepares for protein extraction and storage

# data[].attributes.headline
Remove the caps of the column, place in a tube, and let it drip until in reaches the surface of the HIC matrix

# data[].attributes.subject
Remove the caps of the column, place in a tube, and let it drip until in reaches the surface of the HIC matrix

# data[].attributes.body
Begins the protein separation process

# data[].attributes.headline
Load the supernatant into the top of the column

# data[].attributes.subject
Load the supernatant into the top of the column

# data[].attributes.body
Separates GFP from other proteins

# data[].attributes.headline
Add wash buffer and let the solution pass through the column

# data[].attributes.subject
Add wash buffer and let the solution pass through the column

# data[].attributes.body
Washed away unbound proteins

# data[].attributes.headline
Add TE buffer and let the entire volume flow through the column

# data[].attributes.subject
Add TE buffer and let the entire volume flow through the column

# data[].attributes.body
Removes GFP protein from the column

# data[].attributes.headline
Transfer column to tube just before the GFP drips, and then wrap the tube and refrigerate

# data[].attributes.subject
Transfer column to tube just before the GFP drips, and then wrap the tube and refrigerate

# data[].attributes.body
Collects purified GFP

# data[].attributes.headline
Get the protein samples and prepare them for electrophoresis

# data[].attributes.subject
Get the protein samples and prepare them for electrophoresis

# data[].attributes.body
Analyzes the purity and the size of the GFP protein

# data[].attributes.body
Prepares them for loading onto the gel

# data[].attributes.headline
Add 15 microliters of appropriate loading buffer into each new tube

# data[].attributes.subject
Add 15 microliters of appropriate loading buffer into each new tube

# data[].attributes.body
Increases density of sample and provides visible markers

# data[].attributes.body
Denatures the proteins for separation

# data[].attributes.body
Ensures the proteins have fully denatured

# data[].attributes.body
Prepares for protein separation by electrophoresis

# data[].attributes.headline
Place the top on the chamber and connect to power supply

# data[].attributes.subject
Place the top on the chamber and connect to power supply

# data[].attributes.body
Begins electrophoresis to separate protein by size

# data[].attributes.body
Separates the proteins by size

# data[].attributes.body
Analyzes the protein bands