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Today was the first meeting with our new SV, Dr.Siti Aisyah Mualif in module 2. She explained the topic, objectives, and what we need to do during this second half of the Lab 4 project.

After that, we were divided into a breakout room based on our group to discuss what things are needed to be input to achieve each of the objectives.  The overall session was conducted from 2-3pm.

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This week, each of us tried to find the journals regarding what method is the best to be taken, compare it, then find the enzyme that will suit for the detection of sulfonamide. It was a quite long discussion since the topic is kind of hard to understand. The journals are long enough to get where the point of the mechanism is.

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We help each other to fill up the jam board especially filling up each method with its mechanism. Where personally I had to find out about the electrochemical biosensor, and from my understanding is the electrochemical biosensor should sense and convert the signal effectively and the same signal must trigger the therapeutic release.

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In today's class is we showed Dr.Aisyah about our progress in filling up the jam board. And turns out that we couldn't really explain the details, since we still didn't really get the point of the mechanism. That's why we tried to look for more journals, explanations from youtube, and books as references that the Dr. had passed through group chat.

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After all of the methods had been summarized on the jam board, we all discussed to decide one best mechanism to be applied for sulfonamide biosensor, which's eventually is Electrochemical. Right after that, we also started to write the details on the report because Dr.Aisyah told us so. However, we still couldn't decide on the material for the test strip.

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As usual weekly during an online Lab meetings, we had to report our recent progress either in the form of its report or presentation. Our biosensors design was still rough on a piece of paper, not yet in software or in 3D form.

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Our recent biosensors design by this week was too big and too compact or simple according to Dr.Aisyah. Due to that, my group member asked me to make a new design to either replace or just modify that had been made by Yuni and Syarifah. Yet, I decided to fully redesign. Besides that, during the meeting, some of us got asked by Dr.Aisyah about our understanding of how the mechanism of our biosensors works so expectedly we could be more prepared for the QnA section later.

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In this last session of class, Dr.Aisyah wanted us to show our updated work either the 3D design itself, presentation slides, and report. Yet, we still didn't show the finalized design that I made since it's ongoing and roughly ready to be presented. All in all, we got a lot of insights and comments from Dr. to improve our materials especially for presenting, such as citation mus be included in each sentences that we took from references (although it's showed on slides, not report).

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For the current time being, I got the task to look for how is the form of electrode we can use later in order to can be compact and simple. Yet, by reading the journals, I still couldn't understand the mechanism of how it can detect the analyte dropped on it later. On the other hand, there was also a form of electrode similar to two bars that must be dunk into the tested liquid sample.

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Since this week is off due to the severe flood happening in Malaysia, some of our friends got affected. Thus, we only listed back everything that we needed to search and also what we had found so far.

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Most of us were still confused about what the function of the buffer is, including me. So, we discussed how the overall mechanism of the biosensor is until being able to detect either an analyte contains sulfonamide or not. Besides that, we also divided the task for making presentation slides and new task division where I took in charge to make 3D design of the biosensor device and its strip or electrode.

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We discussed the most important and most confusing part of this project which is Result and Discussion, as well as literature review since it seemed a lot to present later within the limited time, additionally with the explanation of carbon and tyrosinase. Besides that, we also talked about the dimension for biosensor design, which then we took from marketed home-glucose meter sizes.

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We hurried to finish the slides for pre-recording, my part is especially explaining the design of biosensors and the test strip, specification, as well as the dimension. Prior to recording, we showed to the lecturer first to check out and till finally, the recording finished to be compiled by 23rd night.

The recording division:
a. Title: Sharifah✅
b. Introduction/Background:
- Sulfonamides; uses, types, effect Sharifah✅
c. Problem statement: Sharifah✅
d. Objectives: Sharifah✅
e. Literature Review:
- Biosensor Sharifah✅
f. Methodology:
- Materials; enzyme, Screen printed electrode, nanomaterials azra ✅
- Fabrication process Era ✅
- Design consideration (dalam Jamboard) Era ✅
- Biosensor strip and body part Farah ✅
g. Result:
- design summary (in table); name of product, function, type of biosensor and enzyme used, features, specification (dimension and material) farah ✅
- mechanism on how biosensor work, output: azra ✅
h. Discussion:
- Reason why choose that type of sulfonamide, biosensor and the enzyme, nanomaterial, and PLA for body yuni
- advantages and limitation yuni
i. Conclusion - Era

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I was who made the slides first because we were confused what later to show to Dr.Aisyah during online meeting, while the report was also still unorganized and we still didn't really get the point of what had been written there. Thus I initiatively made some slides for at least until the literature review.

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I took charge of designing both the device for the biosensor and the strip. Since I had to do it by myself, I chose a simpler way which is using Tinkercard. It doesn't need a lot of menus or selections to shape the 3D design and almost all the basic shapes are ready to be used. The inspiration of the test strip is that we decided to take from the common screen-printed electrode.

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By this week I had to finish the overall design of the biosensors in order to be put in the pre-recorded slides of the presentation. The very latest feature to consider is what battery to use because we'd like to make the simple, small-sized compact device. Until finally I found while reading which is Lithium Cell that equals AAA. While the overall design is inspired by the glucose meter since it seems fitted to hand to carry on.