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Recombinant enzyme lysosomal acid lipase (LAL)

Specification

Drug Information

Related Products

Product Name:

Recombinant enzyme lysosomal acid lipase (LAL)

Description:

The product is a recombinant form of the enzyme lysosomal acid lipase (LAL). The amino acid sequence for the product is the same as the amino acid sequence for human LAL.

Formula:

C1968H2945N507O551S15

Sequences:

SGGKLTAVDPETNMNVSEIISYWGFPSEEYLVETEDGYILCLNRIPHGRKNHSDKGPKPVVFLQHGLLADSSNWVTNLANSSLGFILADAGFDVWMGNSRGNTWSRKHKTLSVSQDEFWAFSYDEMAKYDLPASINFILNKTGQEQVYYVGHSQGTTIGFIAFSQIPELAKRIKMFFALGPVASVAFCTSPMAKLGRLPDHLIKDLFGDKEFLPQSAFLKWLGTHVCTHVILKELCGNLCFLLCGFNERNLNMSRVDVYTTHSPAGTSVQNMLHWSQAVKFQKFQAFDWGSSAKNYFHYNQSYPPTYNVKDMLVPTAVWSGGHDWLADVYDVNILLTQITNLVFHESIPEWEHLDFIWGLDAPWRLYNKIINLMRKYQ

Molecular Weight:

55000.0 Da

Drug name:

Sebelipase alfa

Applications:

The product is a hydrolytic lysosomal cholesteryl ester and triacylglycerol-specific enzyme indicated for the treatment of patients with a diagnosis of Lysosomal Acid Lipase (LAL) deficiency.

Examples of Clinical Use:

Lysosomal Acid Lipase (LAL) deficiency

Pharmacodynamics :

Not Available

Mechanism of action:

LAL deficiency is an autosomal recessive lysosomal storage disorder characterized by a genetic defect resulting in a marked decrease or loss in activity of the lysosomal acid lipase (LAL) enzyme. The primary site of action of the LAL enzyme is the lysosome, where the enzyme normally causes the breakdown of lipid particles including LDL-c. Deficient LAL enzyme activity results in progressive complications due to the lysosomal accumulation of cholesteryl esters and triglycerides in multiple organs, including the liver, spleen, intestine, and the walls of blood vessels. The resulting lipid accumulation in the liver may lead to increased liver fat content and progression of liver disease, including fibrosis and cirrhosis. Lipid accumulation in the intestinal wall leads to malabsorption and growth failure. In parallel, dyslipidemia due to impaired degradation of lysosomal lipid is common with elevated LDL-c and triglycerides and low HDL-cholesterol (HDL-c). Sebelipase alfa binds to cell surface receptors via glycans expressed on the protein and is subsequently internalized into lysosomes. Sebelipase alfa catalyzes the lysosomal hydrolysis of cholesteryl esters and triglycerides to free cholesterol, glycerol and free fatty acids.

Affected organisms:

Humans and other mammals

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Recombinant enzyme lysosomal acid lipase (LAL) - Therapeutic Proteins

# data[].attributes.attachment_link.description
Specification Drug Information Related Products Product Name: Recombinant enzyme lysosomal acid lipase (LAL) Description: The product is a recombinant form of the enzyme lysosomal acid lipase (LAL). The amino acid sequence for the product is the same as the amino acid sequence for human LAL.

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Human tissue plasminogen activator

Specification

Drug Information

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Product Name:

Human tissue plasminogen activator

Description:

Human tissue plasminogen activator, purified, glycosylated, 355 residues purified from CHO cells. The product is genetically engineered to retain and delete certain portions of human tPA. The product is a deletion mutein of human tPA formed by deleting various amino acids present in endogenous human tPA. The product contains 355 of the 527 amino acids of native human tPA (amino acids 1-3 and 176-527), and retains the activity-related kringle-2 and serine protease domains of human tPA. Three domains are deleted from the product - kringle-1, finger, and epidermal growth factor (EGF).

Formula:

C1736H2671N499O522S22

Sequences:

SYQGNSDCYFGNGSAYRGTHSLTESGASCLPWNSMILIGKVYTAQNPSAQALGLGKHNYCRNPDGDAKPWCHVLKNRRLTWEYCDVPSCSTCGLRQYSQPQFRIKGGLFADIASHPWQAAIFAKHRRSPGERFLCGGILISSCWILSAAHCFQERFPPHHLTVILGRTYRVVPGEEEQKFEVEKYIVHKEFDDDTYDNDIALLQLKSDSSRCAQESSVVRTVCLPPADLQLPDWTECELSGYGKHEALSPFYSERLKEAHVRLYPSSRCTSQHLLNRTVTDNMLCAGDTRSGGPQANLHDACQGDSGGPLVCLNDGRMTLVGIISWGLGCGQKDVPGVYTKVTNYLDWIRDNMRP

Molecular Weight:

39589.6 Da

Synonyms:

Human t-PA (residues 1-3 and 176-527); Reteplase, recombinant; Reteplase,recombinant

Applications:

For lysis of acute pulmonary emboli, intracoronary emboli and management of myocardial infarction.

Examples of Clinical Use:

Acute pulmonary emboli, intracoronary emboli and myocardial infarction

Pharmacodynamics :

The product cleaves the Arg/Val bond in plasminogen to form plasmin. Plasmin in turn degrades the fibrin matrix of the thrombus, thereby exerting its thrombolytic action. This helps eliminate blood clots or arterial blockages that cause myocardial infarction.

Mechanism of action:

Reteplase binds to fibrin rich clots via the fibronectin finger-like domain and the Kringle 2 domain. The protease domain then cleaves the Arg/Val bond in plasminogen to form plasmin. Plasmin in turn degrades the fibrin matrix of the thrombus, thereby exerting its thrombolytic action.

Affected organisms:

Humans and other mammals

Targets:

Target 1. Plasminogen; Target 2. Fibrinogen alpha chain; Target 3. Urokinase plasminogen activator surface receptor; Target 4. Plasminogen activator inhibitor 1

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Human tissue plasminogen activator - Therapeutic Proteins

# data[].attributes.attachment_link.description
Human tissue plasminogen activator, purified, glycosylated, 355 residues purified from CHO cells. The product is genetically engineered to retain and delete certain portions of human tPA. The product is a deletion mutein of human tPA formed by deleting various amino acids present in endogenous human tPA.

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Recombinant analog of the human hormone leptin

Specification

Drug Information

Related Products

Product Name:

Recombinant analog of the human hormone leptin

Description:

The product, a recombinant analog of the human hormone leptin. The product is produced in E. coli and differs from native human leptin by the addition of a methionine residue at its amino terminus.

Formula:

C714H1167N191O221S6

Sequences:

MVPIQKVQDDTKTLIKTIVTRINDISHTQSVSSKQKVTGLDFIPGLHPILTLSKMDQTLAVYQQILTSMPSRNVIQISNDLENLRDLLHVLAFSKSCHLPWASGLETLDSLGGVLEASGYSTEVVALSRLQGSLQDMLWQLDLSPGC

Molecular Weight:

16155.4429 Da

Synonyms:

Metreleptin; Metreleptina; Métréleptine; Metreleptinum; N-Methionylleptin; r-metHuLeptin

Drug name:

Metreleptin

Applications:

The product is indicated as an adjunct to diet as replacement therapy to treat the complications of leptin deficiency in patients with congenital or acquired generalized lipodystrophy.

Examples of Clinical Use:

Congenital or acquired generalized lipodystrophy

Pharmacodynamics :

In patients with leptin deficiency, clinical trials demonstrated that exogenous leptin administration results in weight loss, reduction in mean HbA1c and fasting glucose levels, reduced blood insulin, and reduced triglyceride levels leading to improved insulin sensitivity and reductions in food intake.

Mechanism of action:

Metreleptin functions by binding to and activating the human leptin receptor (ObR), which belongs to the Class I cytokine family of receptors that signals through the JAK/STAT transduction pathway.

Affected organisms:

Humans and other mammals

Targets:

Target 1. Leptin receptor

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Recombinant analog of the human hormone leptin - Therapeutic Proteins

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Specification Drug Information Related Products Product Name: Recombinant analog of the human hormone leptin Description: The product, a recombinant analog of the human hormone leptin. The product is produced in E. coli and differs from native human leptin by the addition of a methionine residue at its amino terminus.

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Specification

Drug Information

Related Products

Product Name:

Human GLP-1 analog

Description:

The product, an analog of human GLP-1 and acts as a GLP-1 receptor agonist. The peptide precursor of the product, produced by a process that includes expression of recombinant DNA in Saccharomyces cerevisiae, has been engineered to be 97% homologous to native human GLP-1 by substituting arginine for lysine at position 34. The product is made by attaching a C-16 fatty acid (palmitic acid) with a glutamic acid spacer on the remaining lysine residue at position 26 of the peptide precursor.

Formula:

C172H265N43O51

Sequences:

HAEGTFTSDVSSYLEGQAAKEEFIAWLVRGRG

Source:

Saccharomyces cerevisiae

Molecular Weight:

3751.2 Da

Synonyms:

Arg34Lys26-(N-ε-(γ-Glu(N-α-hexadecanoyl)))-GLP-1[7-37]; LiraglutidaLiraglutide (genetical recombination); Liraglutide (rDNA origin); Liraglutide recombinant; Liraglutidum; N²⁶-(hexadecanoyl-gamma-glutamyle)-[34-arginine]GLP-1-(7-37)-peptide; N²⁶-(N-Hexadecanoyl-L-gamma-glutamyl)-[34-L-arginine]glucagon-like peptide 1-(7-37)-peptide

Drug name:

Liraglutide

Applications:

For use in/treatment of diabetes mellitus type 2.

Examples of Clinical Use:

Diabetes mellitus type 2

Pharmacodynamics :

The product is a once-daily GLP-1 derivative for the treatment of type 2 diabetes. GLP-1, in its natural form, is short-lived in the body (the half-life after subcutaneous injection is approximately one hour), so it is not very useful as a therapeutic agent. However, the product has a half-life after subcutaneous injection of 11-15 hours, making it suitable for once-daily dosing. The prolonged action of the product is achieved by attaching a fatty acid molecule at one position of the GLP-1 molecule, enabling it to bind to albumin within the subcutaneous tissue and bloodstream. The active GLP-1 is then released from albumin at a slow, consistent rate. Binding with albumin also results in slower degradation and reduced elimination of the product from the circulation by the kidneys compared to GLP-1.

Mechanism of action:

Liraglutide is an acylated GLP-1 (Glucagon-Like Peptide-1) receptor agonist. Liraglutide upregulates intracellular cAMP resulting in the release of insulin given elevated blood glucose concentrations. Glucagon secretion is also decreased in a glucose-dependent fashion by liraglutide.

Affected organisms:

Humans and other mammals

Targets:

Target 1. Glucagon-like peptide 1 receptor; Enzyme 1. Dipeptidyl peptidase 4; Enzyme 2. Neprilysin

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Human GLP-1 analog - Therapeutic Proteins

# data[].attributes.attachment_link.description
The product, an analog of human GLP-1 and acts as a GLP-1 receptor agonist. The peptide precursor of the product, produced by a process that includes expression of recombinant DNA in Saccharomyces cerevisiae, has been engineered to be 97% homologous to native human GLP-1 by substituting arginine for lysine at position 34.

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Human recombinant alpha-L-iduronidase (628 residues)

Specification

Drug Information

Related Products

Product Name:

Human recombinant alpha-L-iduronidase (628 residues)

Description:

Human recombinant alpha-L-iduronidase, 628 residues (mature form), produced by recombinant DNAtechnology in a Chinese hamster ovary cell line. The product is a glycoprotein with a molecular weight of approximately 83 kD. The predicted amino acid sequence of the recombinant form, as well as the nucleotide sequence that encodes it, are identical to a polymorphic form of human a-L-iduronidase. It contains 6 N-linked oligosaccharide modification sites.

Formula:

C3160H4848N898O881S12

Sequences:

APHLVQVDAARALWPLRRFWRSTGFCPPLPHSQADQYVLSWDQQLNLAYVGAVPHRGIKQVRTHWLLELVTTRGSTGRGLSYNFTHLDGYLDLLRENQLLPGFELMGSASGHFTDFEDKQQVFEWKDLVSSLARRYIGRYGLAHVSKWNFETWNEPDHHDFDNVSMTMQGFLNYYDACSEGLRAASPALRLGGPGDSFHTPPRSPLSWGLLRHCHDGTNFFTGEAGVRLDYISLHRKGARSSISILEQEKVVAQQIRQLFPKFADTPIYNDEADPLVGWSLPQPWRADVTYAAMVVKVIAQHQNLLLANTTSAFPYALLSNDNAFLSYHPHPFAQRTLTARFQVNNTRPPHVQLLRKPVLTAMGLLALLDEEQLWAEVSQAGTVLDSNHTVGVLASAHRPQGPADAWRAAVLIYASDDTRAHPNRSVAVTLRLRGVPPGPGLVYVTRYLDNGLCSPDGEWRRLGRPVFPTAEQFRRMRAAEDPVAAAPRPLPAGGRLTLRPALRLPSLLLVHVCARPEKPPGQVTRLRALPLTQGQLVLVWSDEHVGSKCLWTYEIQFSQDGKAYTPVSRKPSTFNLFVFSPDTGAVSGSYRVRALDYWARPGPFSDPVPYLEVPVPRGPPSPGNP

Molecular Weight:

69899.4 Da

Synonyms:

alpha-L-Idosiduronase; Human Recombinant alpha-L-iduronidase; Laronidase; Laronidase (genetical recombination)

Applications:

For the treatment of mucopolysaccharidosis

Examples of Clinical Use:

Mucopolysaccharidosis

Pharmacodynamics :

The product is used to treat mucopolysaccharide storage disorders (specifically mucopolysaccharidosis 1 or Hurlers syndrome) caused by deficiencies of alpha-L-iduronidase. Reduced or absent a-L-iduronidase activity results in the accumulation of the GAG substrates, dermatan sulfate and heparan sulfate, throughout the body and leads to widespread cellular, tissue, and organ dysfunction.

Mechanism of action:

Laronidase catalyses the hydrolysis of terminal alpha-L-iduronic acid residues of dermatan sulfate and heparin sulfate.

Affected organisms:

Humans and other mammals

Targets:

Target 1. Iduronic acid

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Human recombinant alpha-L-iduronidase (628 residues) - Therapeutic Proteins

# data[].attributes.attachment_link.description
Human recombinant alpha-L-iduronidase, 628 residues (mature form), produced by recombinant DNAtechnology in a Chinese hamster ovary cell line. The product is a glycoprotein with a molecular weight of approximately 83 kD. The predicted amino acid sequence of the recombinant form, as well as the nucleotide sequence that encodes it, are identical to a polymorphic form of human a-L-iduronidase.

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Recombinant human insulin analogue (has a lysine at residuce B28 and proline at B29)

Specification

Drug Information

Related Products

Product Name:

Recombinant human insulin analogue (has a lysine at residuce B28 and proline at B29)

Description:

The product is a recombinant human insulin analogue produced in a specialized laboratory strain of Escherischia coli. Plasmid DNA transfected into the bacteria encodes for an analogue of human insulin that has a lysine at residuce B28 and proline at B29; these residues are reversed in endogenous human insulin. Reversal of these amino acid residues produces a rapid-acting insulin analogue.

Formula:

C257H387N65O76S6

Sequences:

GIVEQCCTSICSLYQLENYCNFVNQHLCGSHLVEALYLVCGERGFFYTKPT

Molecular Weight:

5808.0 Da

Synonyms:

Insulin lispro (genetical recombination); Insulin lispro (rDNA origin); Insulin lispro protamine; Insulin lispro protamine recombinant; Insulin lispro recombinant; Insulin,lispro,human/rDNA; Insulin,lispro,protamine/rDNA

Drug name:

Insulin Lispro

Applications:

For the treatment of Type 1 or 2 diabetes mellitus. To be used in conjunction with an intermediate or long-acting insulin except when used in a continuous insulin infusion pump.

Examples of Clinical Use:

Type 1 or 2 diabetes mellitus

Pharmacodynamics :

Insulin is a natural hormone produced by beta cells of the pancreas. In non-diabetic individuals, a basal level of insulin is supplemented with insulin spikes following meals. Increased insulin secretion following meals is responsible for the metabolic changes that occur as the body transitions from a postabsorptive to absorptive state. Insulin promotes cellular uptake of glucose, particularly in muscle and adipose tissues, promotes energy storage via glycogenesis, opposes catabolism of energy stores, increases DNA replication and protein synthesis by stimulating amino acid uptake by liver, muscle and adipose tissue, and modifies the activity of numerous enzymes involved in glycogen synthesis and glycolysis. Insulin also promotes growth and is required for the actions of growth hormone (e.g. protein synthesis, cell division, DNA synthesis). The product is a rapid-acting insulin analogue used to mimic postprandial insulin spikes in diabetic individuals. The onset of action of the product is 10-15 minutes. Its activity peaks 60 minutes following subcutaneous injection and its duration of action is 4-5 hours. Compared to regular human insulin, the product has a more rapid onset of action and a shorter duration of action. The product is also shown to be equipotent to human insulin on a molar basis.

Mechanism of action:

Insulin lispro binds to the insulin receptor (IR), a heterotetrameric protein consisting of two extracellular alpha units and two transmembrane beta units. The binding of insulin to the alpha subunit of IR stimulates the tyrosine kinase activity intrinsic to the beta subunit of the receptor. The bound receptor autophosphorylates and phosphorylates numerous intracellular substrates such as insulin receptor substrates (IRS) proteins, Cbl, APS, Shc and Gab 1. Activation of these proteins leads to the activation of downstream signaling molecules including PI3 kinase and Akt. Akt regulates the activity of glucose transporter 4 (GLUT4) and protein kinase C (PKC), both of which play critical roles in metabolism and catabolism. In humans, insulin is stored in the form of hexamers; however, only insulin monomers are able to interact with IR. Reversal of the proline and lysine residues at positions B28 and B29 of native insulin eliminates hydrophobic interactions and weakens some of the hydrogen bonds that contribute to the stability of the insulin dimers that comprise insulin hexamers. Hexamers of insulin lispro are produced in the presence of zinc and m-cresol. These weakly associated hexamers quickly dissociate upon subcutaneous injection and are absorbed as monomers through vascular endothelial cells. These properties give insulin lispro its fast-acting properties.

Affected organisms:

Humans and other mammals

Targets:

Target 1. Insulin receptor; Target 2. Insulin-like growth factor 1 receptor; Enzyme 1. Cytochrome P450 1A2.

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Recombinant human insulin analogue (has a lysine at residuce B28 and proline at B29) - Therapeutic Proteins

# data[].attributes.attachment_link.description
The product is a recombinant human insulin analogue produced in a specialized laboratory strain of Escherischia coli. Plasmid DNA transfected into the bacteria encodes for an analogue of human insulin that has a lysine at residuce B28 and proline at B29; these residues are reversed in endogenous human insulin.

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51 residue peptide hormone

Specification

Drug Information

Related Products

Product Name:

51 residue peptide hormone

Description:

The product is a 51 residue peptide hormone, composed of two amino acid chains covalently linked by disulfide bonds. The structure is identical to native human insulin. Recombinant insulin is synthesized by recombinant DNA techncology. Inserting the human insulin gene into the Escherichia coli bacteria or Saccharomyces cerevisiae produces insulin for human use.

Formula:

C257H383N65O77S6

Sequences:

GIVEQCCTSICSLYQLENYCNFVNQHLCGSHLVEALYLVCGERGFFYTPKT

Molecular Weight:

5808.0 Da

Synonyms:

High molecular weight insulin human; Human insulinhuman insulin (rDNA); Insulin (human); Insulin human [rDNA origin]; Insulin Human Regular; Insulin human regular (rDNA); Insulin human, rDNA origin; Insulin recombinant human; Insulin recombinant purified human; Insulin regular; Insulin, human; Regular Insulin, human

Drug name:

Insulin Human

Applications:

Indicated as an adjunct to diet and exercise to improve glycemic control in adults and children with type 1 and type 2 diabetes mellitus.

Examples of Clinical Use:

Type 1 or 2 diabetes mellitus

Pharmacodynamics :

Insulin regular is a short-acting insulin. When subcutaneously administered, the onset of action (as evidenced by a decrease in glucose level) occurs 30 minutes post-dose. Maximal effect occurs between 1.5 and 3.5 hours post-dose. The glucose-lowering effect occurs 8 hours post-dose. Compared to other rapid-acting insulin analogs, insulin regular has a slower onset of action and longer duration of action.

Mechanism of action:

The primary activity of insulin is the regulation of glucose metabolism. Insulin promotes glucose and amino acid uptake into muscle and adipose tissues, and other tissues except brain and liver. It also has an anabolic role in stimulating glycogen, fatty acid, and protein synthesis. Insulin inhibits gluconeogenesis in the liver.Insulin binds to the insulin receptor (IR), a heterotetrameric protein consisting of two extracellular alpha units and two transmembrane beta units. The binding of insulin to the alpha subunit of IR stimulates the tyrosine kinase activity intrinsic to the beta subunit of the receptor. The bound receptor is able to autophosphorylate and phosphorylate numerous intracellular substrates such as insulin receptor substrates (IRS) proteins, Cbl, APS, Shc and Gab 1. These activated proteins, in turn, lead to the activation of downstream signaling molecules including PI3 kinase and Akt. Akt regulates the activity of glucose transporter 4 (GLUT4) and protein kinase C (PKC) which play a critical role in metabolism and catabolism.

Affected organisms:

Humans and other mammals

Targets:

Target 1. Insulin receptor; Target 2. Insulin-like growth factor 1 receptor; Target 3. Retinoblastoma-associated protein; Target 4. Cathepsin D; Target 5. Insulin-degrading enzyme; Target 6. Neuroendocrine convertase 2; Target 7. Carboxypeptidase E; Target 8. Neuroendocrine convertase 1; Target 9. Protein NOV homolog; Target 10. Low-density lipoprotein receptor-related protein 2; Target 11. Insulin-like growth factor-binding protein 7; Target 12. Synaptotagmin-like protein 4; Enzyme 1. Cytochrome P450 1A2

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

# data[].attributes.attachment_link.title
51 residue peptide hormone - Therapeutic Proteins

# data[].attributes.attachment_link.description
The product is a 51 residue peptide hormone, composed of two amino acid chains covalently linked by disulfide bonds. The structure is identical to native human insulin. Recombinant insulin is synthesized by recombinant DNA techncology. Inserting the human insulin gene into the Escherichia coli bacteria or Saccharomyces cerevisiae produces insulin for human use.

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Human insulin analogue (the amino acid arginine at position B3 is replaced by lysine and the lysine at position B29 is replaced by glutamic acid)

Specification

Drug Information

Related Products

Product Name:

Human insulin analogue (the amino acid arginine at position B3 is replaced by lysine and the lysine at position B29 is replaced by glutamic acid)

Description:

The product is a biosynthetic, rapid-acting human insulin analogue produced in a non-pathogenic laboratory strain of Escherichia coli (K12). This recombinant hormone differs from native human insulin in that the amino acid arginine at position B3 is replaced by lysine and the lysine at position B29 is replaced by glutamic acid. These structural modifications decrease hexamer formation, stabilize the product monomers and increase the rate of absorption and onset of action compared to human insulin.

Formula:

C258H384N64O78S6

Sequences:

GIVEQCCTSICSLYQLENYCNFVKQHLCGSHLVEALYLVCGERGFFYTPET

Source:

Escherichia coli (K12)

Molecular Weight:

5823.0 Da

Synonyms:

Insulin Glulisine (recombinant DNA origin); Insulin glulisine recombinant

Drug name:

Insulin Glulisine

Applications:

For the treatment of Type 1 and 2 diabetes mellitus. Should be used in regimens including a long-acting or basal insulin analogue unless it is used in a continuous infusion pump. May be used with oral antidiabetic agents.

Examples of Clinical Use:

Type 1 or 2 diabetes mellitus

Pharmacodynamics :

Insulin is a natural hormone produced by beta cells of the pancreas. In non-diabetic individuals, a basal level of insulin is supplemented with insulin spikes following meals. Postprandial insulin spikes are responsible for the metabolic changes that occur as the body transitions from a postabsorptive to absorptive state. Insulin promotes cellular uptake of glucose, particularly in muscle and adipose tissues, promotes energy storage via glycogenesis, opposes catabolism of energy stores, increases DNA replication and protein synthesis by stimulating amino acid uptake by liver, muscle and adipose tissue, and modifies the activity of numerous enzymes involved in glycogen synthesis and glycolysis. Insulin also promotes growth and is required for the actions of growth hormone (e.g. protein synthesis, cell division, DNA synthesis). The product is a rapid-acting insulin analogue used to mimic postprandial insulin spikes in diabetic individuals. The onset of action of the product is approximately 15 minutes. Its activity peaks 60 minutes following subcutaneous injection and its duration of action is 2-4 hours.

Mechanism of action:

Insulin glulisine binds to the insulin receptor (IR), a heterotetrameric protein consisting of two extracellular alpha units and two transmembrane beta units. The binding of insulin to the alpha subunit of IR stimulates the tyrosine kinase activity intrinsic to the beta subunit of the receptor. The bound receptor autophosphorylates and phosphorylates numerous intracellular substrates such as insulin receptor substrates (IRS) proteins, Cbl, APS, Shc and Gab 1. Activation of these proteins leads to the activation of downstream signaling molecules including PI3 kinase and Akt. Akt regulates the activity of glucose transporter 4 (GLUT4) and protein kinase C (PKC), both of which play critical roles in metabolism and catabolism. In humans, insulin is stored in the form of hexamers; however, only insulin monomers are able to interact with IR. Substitution of the arginine at position B3 for lysine and replacement of the B29 lysine with glutamic acid decreases the propensity to form hexamers, stabilizes the hormone in monomeric form and results in a rapid rate of absorption and short duration of action.

Affected organisms:

Humans and other mammals

Targets:

Target 1. Insulin receptor; Enzyme 1. Cytochrome P450 1A2.

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

# data[].attributes.attachment_link.title
Human insulin analogue (the amino acid arginine at position B3 is replaced by lysine and the lysine at position B29 is replaced by glutamic acid) - Therapeutic Proteins

# data[].attributes.attachment_link.description
The product is a biosynthetic, rapid-acting human insulin analogue produced in a non-pathogenic laboratory strain of Escherichia coli (K12). This recombinant hormone differs from native human insulin in that the amino acid arginine at position B3 is replaced by lysine and the lysine at position B29 is replaced by glutamic acid.

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Creative BioMart provides customers with comprehensive endotoxin research services.Comprehensive,
one-stop service for a faster and more efficient experience.

Creative BioMart offers a variety of endotoxin-related products. Including endotoxin testing kits, endotoxin removal kits, endotoxin standards, purified LPS and its derivatives, endotoxin-free reagents, and consumables.

Creative BioMart provides customers with comprehensive endotoxin research services.Comprehensive,
one-stop service for a faster and more efficient experience.

Endotoxin Testing Services
Creative BioMart provides a full range of services for endotoxin testing. Our endotoxin testing services cover a wide range of methods and are available for a wide range of samples to provide comprehensive and efficient help for your experiments.

Endotoxin Removal Services
Creative BioMart provides a full range of services for endotoxin removal. Our endotoxin removal services cover a wide range of methods and are available for a wide range of samples to provide comprehensive and efficient help for your experiments.

Low Endotoxin Recovery
Low Endotoxin Recovery (LER) has a great impact on the detection of endotoxin, so to determine the accuracy of endotoxin detection, LER interference should be ruled out. Creative BioMart provides a hold-time studies to exclude LER interference services.

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Creative BioMart provides customers with comprehensive endotoxin research services.Comprehensive, one-stop service for a faster and more efficient experience. Creative BioMart offers a variety of endotoxin-related products. Including endotoxin testing kits, endotoxin removal kits, endotoxin standards, purified LPS and its derivatives, endotoxin-free reagents, and consumables.

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Endotoxin Removal Services in Recombinant Protein

# data[].attributes.attachment_link.title
Endotoxin testing Services - Creative BioMart

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Creative BioMart offers you TAL / LAL Reagent for endotoxin detection experiments. TAL / LAL Reagent can be used as a supplement to the detection reagents in the Endotoxin Testing Kit. It can also be purchased separately and used in conjunction with buffers and reaction tubes.

TAL / LAL reagents provided by Creative BioMart include a variety of specifications: vials, ampoules, vacuum test tubes, etc. In addition, TAL / LAL reagent also includes a variety of detection methods, such as Gel Clot LAL Assay, Chromogenic LAL Assay and Chromogenic LAL Assay.

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Creative BioMart offers you TAL / LAL Reagent for endotoxin detection experiments. TAL / LAL Reagent can be used as a supplement to the detection reagents in the Endotoxin Testing Kit. It can also be purchased separately and used in conjunction with buffers and reaction tubes.

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Creative BioMart provides you with endotoxin-related Supporting reagents, in order to complete the detection and removal of endotoxins more accurately and without interference.

Mainly provide Tris HCl Buffer, MgCl ₂, Endotoxin-free Blood Anticoagulant，Glucan Blocker and Dispersing Agent.

# data[].attributes.attachment_link.description
Creative BioMart provides you with endotoxin-related Supporting reagents, in order to complete the detection and removal of endotoxins more accurately and without interference. Mainly provide Tris HCl Buffer, MgCl 2, Endotoxin-free Blood Anticoagulant，Glucan Blocker and Dispersing Agent.

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Endotoxin (LPS) preparations are widely used to study the structure, metabolism, immunology, and biosynthesis of LPS. LPS is also used to induce the synthesis and secretion of growth-promoting factors such as interleukins. By combining LPS with FITC, TRITC, and TNP, it can be used to study intracellular responses such as immune response to LPS.

Creative BioMart offers a variety of LPS products, including a variety of bacterial sources, and various labeled LPS.

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Endotoxin (LPS) preparations are widely used to study the structure, metabolism, immunology, and biosynthesis of LPS. LPS is also used to induce the synthesis and secretion of growth-promoting factors such as interleukins. By combining LPS with FITC, TRITC, and TNP, it can be used to study intracellular responses such as immune response to LPS.

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Home >> Products >> Endotoxin-free Purification Resins

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Home >> Products >> Endotoxin-free Purification Kits

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Epigenetic Assays | Creative BioMart - Assay Kit

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Metabolism and Endocrinology Assays | Creative BioMart - Assay Kit

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Enzyme Activity Assays | Creative BioMart - Assay Kit

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Performance of assays kits purchased from manufacturers is highly recommended to be validated before use. Variations caused by lot-lot difference might lead to inconsistent assay results, which could be avoid beforehand by kit validation. Some manufacturers might implement rigorous quality control, while some might not. Necessity of validating the performance of assay kits is therefore apparent. Creative BioMart provides validation service to check the quality of your assay kits with regards to specificity, sensitivity, reproducibility, and linearity.

Assay Kit Validation Workflow

We are able to carry out validation with various types of assay kits, including ELISA kit, enzyme activity assay kit, quantification kit, sample preparation kit, et al. Positive control and negative control samples will be purchased if they are available in market. Otherwise customers are required to provide them for validation. Statistical analysis is presented in the validation report to conclude the performance of your assay kits.

Creative BioMart is experienced on assay kit validation and has gained great reputation in market. We are glad to help you ensure the quality of commercial assay kits, thus allowing smooth experiments. If you are looking for assay kit validation solutions, please contact us for custom service.

Inquiry

We are here to answer any question you may have

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Kit Validation | Creative BioMart - Assay Kit

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Performance of assays kits purchased from manufacturers is highly recommended to be validated before use. Variations caused by lot-lot difference might lead to inconsistent assay results, which could be avoid beforehand by kit validation. Some manufacturers might implement rigorous quality control, while some might not.

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Commercial assay kits are extensively used by scientists and researchers in a broad range of areas, such as biological research, drug development, medical test, food quality control, et al. We are devoted to development of novel assay kits that fill up the blank in market or that serve our valued customers. If the assay kit you are looking for is not available on market yet, please feel free to send a development request to Creative BioMart - Assay Kit. We are ready to help you design and produce your own kits as per your requirement.

Assay Kit Development Workflow

Key Features

We provide custom assay kit service to cover activity determination of different types of enzymes, including but not limited to oxidoreductases, transferases, hydrolases, lyases, isomerases, and ligases. Metabolites and hormones are associated with a number of urgent health issues. Detection of theses biomarkers therefore enables research on metabolic syndrome diagnosis. We comply comprehensive strategies to fulfill requirements of accurate and precise assay of biomarkers of interest. Moreover, assay kits which are used to detect cellular components, biochemical processes, epigenetic modifications, and cell signaling pathways, can be developed and produced by our professional team as well.

Creative BioMart has grown to the global leader in protein and enzyme sicence since last decade. Our collaborators in research institutes, industries, and governments think highly of our top services and products. Our mission is to make your assay quick, easy to handle, cost-effective, accurate, and reproducible. Please contact us if you plan to creative your own assay kit.

Inquiry

We are here to answer any question you may have

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Kit Development | Creative BioMart - Assay Kit

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Commercial assay kits are extensively used by scientists and researchers in a broad range of areas, such as biological research, drug development, medical test, food quality control, et al. We are devoted to development of novel assay kits that fill up the blank in market or that serve our valued customers.

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Food and Cosmetics Related Substances Test

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Food and Cosmetics Related Substances Test

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Ingredients and additives in food and cosmetics constantly draw public attention due to their potential impact on human health. Quality control plays a critical role before food product and cosmetics become available in the market. Creative BioMart endeavors to provide food and cosmetics related substances testing service using our assay kits to ensure test reliability, sensitivity, and specificity.

Our tests by assay kit cover a wide range of substances present or potentially present in food and cosmetics. Generally, your food product sample is processed and subject to quantification of acidity, hydrogen peroxide, peroxide value, carbonyl value, iodine value, soap content, unsaponifiable matter, pesticide residue, sibutramine et al. We also provide quick detection of contaminant microorganisms found in food product, such as Aspergillus, Penicillium, E. coli, et al. It is also available to test cosmetics sample concerning D-sorbitol, nitroimidazole, minoxidil, hydroquinone, sulfonamides, formaldehyde, boric acid, lincomycin, et al. As heavy metal accumulation prevalent in plants and animals, it comes imperative to assure the safe level of them in food and cosmetics. We are experienced in quick test of calcium, copper, iron, magnesium, cadmium, mercury, aluminum, nickel, cobalt, zinc, lead, tin, manganese, chromium, selenium, et al.

Creative BioMart is proud to serve you with sensitive and reliable food and cosmetics related substances testing in a short turnaround time. Please feel free to contact us if you plan to have your sample tested.

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Food and Cosmetics Related Substances Test | Creative BioMart - Assay Kit

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Ingredients and additives in food and cosmetics constantly draw public attention due to their potential impact on human health. Quality control plays a critical role before food product and cosmetics become available in the market. Creative BioMart endeavors to provide food and cosmetics related substances testing service using our assay kits to ensure test reliability, sensitivity, and specificity.

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Detection Assays | Creative BioMart - Assay Kit

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Cellular and Biochemical Assays | Creative BioMart - Assay Kit

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Metabolism and Endocrinology Assays | Creative BioMart - Assay Kit

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| Interleukin-4 is a pleiotropic cytokine produced primarily by activated T lymphocytes, basophils and mast cells.

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Specification

Drug Information

Related Products

Product Name:

Interleukin-12 (IL-12)

Description:

Interleukin-12 is a disulfide-linked heterodimeric protein (p70), composed of two subunits, p35 and p40, which are encoded by two different genes. Accumulating data indicate that p40 secretion precedes that of IL-12 expression. In addition, to its ability to covalently bind to p35 to form IL-12, p40 can bind to p19 to form IL-23. Recombinant human IL-12 p40 is a 40 kDa protein containing 306 amino acid residues.

Introduction:

As an influential heterodimeric pleiotropic cytokine, IL-12 is key in regulating certain immune responses. This is done through its regulation of adaptive type-1 cell-mediated immunity: a pathway that aids in protection against many viral agents. It is primarily produced through the stimulation of white blood cells in human tissue known as macrophages, monocytes or dendritic cells and occurs naturally in the body, as a response to factors such as invading pathogens, cellular damage interacting with activated T-cells. Having a large impact over both T-cells and natural killer cells (or NK cells), IL-12 has been identified as having strongly positive applications for fighting cancer.

Molecular Weight:

40 kDa

Synonyms:

CLMF; IL-12; NKSF; NSC672423; Ro-24-7472; YM01C, Cytotoxic lymphocyte maturation factor; Edodekin alfa (Prop INNM; USAN); Natural killer stimulatory factor;

Biological Activity:

Human IL-12 p40 has no IL-12 (p70) antagonist activity.

Purity:

>97% by SDS-PAGE and HPLC analysis.

Formulation:

Sterile filtered and then the protein was lyophilized in 1 x PBS pH 6.8.

Storage:

The lyophilized protein is stable for a few weeks at room temperature, but best stored at -80 centigrade. Reconstituted IL-12 p40 should be stored in working aliquots at -80 centigrade.

Reconstitution:

We recommend a quick spin followed by reconstitution in 200 ul of sterile water for injection for 0.5 mg/ml concentration . This solution can then be diluted into other aqueous buffers and stored at 4 centigrade for 3 months or below -10 centigrade for future use.

Endotoxin Level:

<0.1 ng per μg (1EU/μg).

Applications:

Having a large impact over both T-cells and natural killer cells (or NK cells), IL-12 has been identified as having strongly positive applications for fighting cancer.

Examples of Clinical Use:

Fighting cancer

Affected organisms:

Humans

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Interleukin-12 (IL-12) - Therapeutic Proteins

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Interleukin-12 is a disulfide-linked heterodimeric protein (p70), composed of two subunits, p35 and p40, which are encoded by two different genes. Accumulating data indicate that p40 secretion precedes that of IL-12 expression. In addition, to its ability to covalently bind to p35 to form IL-12, p40 can bind to p19 to form IL-23.

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| Interleukin-4 is a pleiotropic cytokine produced primarily by activated T lymphocytes, basophils and mast cells.

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Specification

Drug Information

Related Products

Product Name:

Interferon gamma (IFN-g)

Description:

Interferon gamma (IFN-g) is produced by lymphocytes which have been activated by specific antigens or mitogens.

Introduction:

IFN-g, in addition to having antiviral activity, has important immune-regulatory functions, it is a potent activator of macrophages, and has anti-proliferative effects on transformed cells and it can potentiate the anti-viral and anti-tumor effects of type I interferons. IFN-g mouse recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 134 amino acids and having a molecular mass of 15.6 kDa. The IFN-gamma is purified by proprietary chromatographic techniques.

Sequences:

MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC

Molecular Weight:

15.6 kDa

Synonyms:

IFN-g; Immune interferon; type II interferon; T-cell interferon;

Biological Activity:

The specific activity as determined in a viral resistance assay is < 0.1 ng/ml, corresponding to a specific activity of 1 x 10＾7 U/mg.

Purity:

>95% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE

Formulation:

Lyophilized from a 0.2 μm filtered concentrated (1 mg/ml) solution in PBS, pH 7.4 and 5% trehalose. Sterile filtered white lyophilized (freeze-dried) powder.

Storage:

Lyophilized IFN-g although stable at room temperature for 3 weeks, should be stored desiccated below -20 centigrade. Upon reconstitution IFN-g should be stored at 2-8 centigrade between up to 7 days and for future use below -20 centigrade. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Reconstitution:

It is recommended to reconstitute the lyophilized IFN-g in sterile distilled water not less than 100 μg/ml which can then be further diluted to other aqueous solutions.

Applications:

IFN-g, in addition to having antiviral activity, has important immune-regulatory functions, it is a potent activator of macrophages, and has anti-proliferative effects on transformed cells and it can potentiate the anti-viral and anti-tumor effects of type I interferons.

Examples of Clinical Use:

Anti-viral and anti-tumor effects

Affected organisms:

Humans

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Interferon gamma (IFN-g) - Therapeutic Proteins

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Specification Drug Information Related Products Product Name: Interferon gamma (IFN-g) Description: Interferon gamma (IFN-g) is produced by lymphocytes which have been activated by specific antigens or mitogens. Introduction: IFN-g, in addition to having antiviral activity, has important immune-regulatory functions, it is a potent activator of macrophages, and has anti-proliferative effects on transformed cells and it can potentiate the anti-viral and anti-tumor effects of type I interferons.

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Pseudomonas Exotoxin A from Pseudomonas Aeruginosa

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Pseudomonas Exotoxin A from Pseudomonas Aeruginosa

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| Interleukin-4 is a pleiotropic cytokine produced primarily by activated T lymphocytes, basophils and mast cells.

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Pseudomonas Exotoxin A from Pseudomonas Aeruginosa

Specification

Drug Information

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Product Name:

Pseudomonas Exotoxin A from Pseudomonas Aeruginosa

Description:

Pseudomonas exotoxin A is secreted by Pseudomonas aeruginosa. It is a member of the family mono-ADP-ribosyltransferases.

Introduction:

The Pseudomonas exotoxin (or exotoxin A) is an exotoxin produced by Pseudomonas aeruginosa. Vibrio cholerae produces a similar protein called the Cholix toxin (Q5EK40). It inhibits elongation factor-2. It does so by ADP-ribosylation of EF2 using NAD+. This then causes the elongation of polypeptides to cease. This mechanism is similar to that of diphtheria toxin. It has been investigated as a treatment for hepatitis B and cancer.

Source:

Pseudomonas aeruginosa

Synonyms:

Pseudomonas Exotoxin A from Pseudomonas Aeruginosa;

Formulation:

Lyophilized powder containing Tris buffer, EDTA, NaCl and lactose.

Storage:

Store at 2-8 centigrade.

Drug name:

Alvircept sudotox

Applications:

It has been investigated as a treatment for hepatitis B and cancer.

Examples of Clinical Use:

As a treatment for hepatitis B and cancer.

Affected organisms:

Humans

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Pseudomonas Exotoxin A from Pseudomonas Aeruginosa - Therapeutic Proteins

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Specification Drug Information Related Products Product Name: Pseudomonas Exotoxin A from Pseudomonas Aeruginosa Description: Pseudomonas exotoxin A is secreted by Pseudomonas aeruginosa. It is a member of the family mono-ADP-ribosyltransferases. Introduction: The Pseudomonas exotoxin (or exotoxin A) is an exotoxin produced by Pseudomonas aeruginosa. Vibrio cholerae produces a similar protein called the Cholix toxin (Q5EK40).

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| Interleukin-4 is a pleiotropic cytokine produced primarily by activated T lymphocytes, basophils and mast cells.

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Specification

Drug Information

Related Products

Product Name:

Soluble CD4 (sCD4)

Description:

CD4 is a transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.

Introduction:

CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost, giving rise to single positive (SP) CD4+ or CD8+ mature T cells. CD4+ SP cells, also known as T helper cells, further differentiate into multiple subsets of CD4+ cells including Th1, Th2, Th9, Th17,Th22, Tfh, and Treg cells which regulate humoral and cellular immunity. In human, CD4 is additionally expressed on macrophages, neutrophils, monocytes, NK cells, and neurons and glial cells in the brain. CD4 binds directly to MHC class II molecules on antigen presenting cells. This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction. CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a coreceptor for the gp120 surface glycoprotein of HIV-1.

Source:

Baculovirus infected Sf21 cells

Molecular Weight:

40 kDa

Synonyms:

CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4;

Biological Activity:

Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells. When 5 x 10＾4 cells/well are added to human recombinant CD4 coated plates (2.5 μg/mL, 100 μL/well), approximately 50-80% of the cells will adhere after 1 hour at 37 centigrade.

Purity:

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Formulation:

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution:

Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Endotoxin Level:

<0.10 EU per 1 μg of the protein by the LAL method.

Applications:

CD4 binds directly to MHC class II molecules on antigen presenting cells. This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction. CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a coreceptor for the gp120 surface glycoprotein of HIV-1.

Examples of Clinical Use:

Formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction.

Affected organisms:

Humans

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Soluble CD4 (sCD4) - Therapeutic Proteins

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Specification Drug Information Related Products Product Name: Soluble CD4 (sCD4) Description: CD4 is a transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.

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| Interleukin-4 is a pleiotropic cytokine produced primarily by activated T lymphocytes, basophils and mast cells.

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Platelet-Derived Growth Factor-AB (PDGF-AB)

Specification

Drug Information

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Product Name:

Platelet-Derived Growth Factor-AB (PDGF-AB)

Description:

PDGF-AB isomer is found to be associated with angiogenesis and proliferation. Potency: 10-100 ng/mL ED50/EC50.

Introduction:

Platelet-Derived Growth Factor (PDGF) is a cationic polypeptide and a constituent of α-granules. PDGF is composed of chain A and B linked together by a disulphide bridge. PDGF heterodimers occurs as PDGF-AB and homodimers exists as FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD). The PDGFB gene is localized to pericytes in the brain and is mapped to human chromosome 7p22.3. Cellular actions of platelet-derived growth factor (PDGF) include chemoattraction and activation of neutrophils, monocytes, and fibroblasts. PDGF appears to play a crucial role in the cellular response to tissue injury and is critically involved in the pathological progression of atherosclerosis. PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair.

Molecular Weight:

27-31 kDa

Biological Activity:

The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.

Purity:

≥97% (SDS-PAGE and N-terminal analysis)

Formulation:

Lyophilized from a 0.2 μm filtered solution in 40% acetonitrile and 0.1% trifluoroacetic acid.

Storage:

Store at -20 centigrade, avoid repeated freeze/thaw cycles.

Applications:

Platelet-Derived Growth Factor-AB human has been used in luciferase reporter assay.

Examples of Clinical Use:

Be associated with angiogenesis and proliferation.

Affected organisms:

Humans

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Platelet-Derived Growth Factor-AB (PDGF-AB) - Therapeutic Proteins

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Specification Drug Information Related Products Product Name: Platelet-Derived Growth Factor-AB (PDGF-AB) Description: PDGF-AB isomer is found to be associated with angiogenesis and proliferation. Potency: 10-100 ng/mL ED50/EC50. Introduction: Platelet-Derived Growth Factor (PDGF) is a cationic polypeptide and a constituent of α-granules. PDGF is composed of chain A and B linked together by a disulphide bridge.

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| Interleukin-4 is a pleiotropic cytokine produced primarily by activated T lymphocytes, basophils and mast cells.

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Human Interleukin 4 (IL-4)

Specification

Drug Information

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Product Name:

Human Interleukin 4 (IL-4)

Description:

Interleukin-4 is a pleiotropic cytokine produced primarily by activated T lymphocytes, basophils and mast cells.

Introduction:

Multiple immune response-modulating functions are performed by IL-4 on a variety of cell types and it has an important role in the regulator of isotype switching, induction of IgE production in B lymphocytes and differentiation of precursor T helper cells. IL-4 binds to both membrane-bound and secreted soluble IL-4 receptors. Interleukin-4 made in the laboratory is used as a biological response modifier to boost the immune system in cancer therapy.

Synonyms:

BCGF; BCDF; B cell stimulating factor; BSF-1; Lymphocyte stimulatory factor 1; IL-4; MGC79402; Binetrakin; Pitrakinra;

Biological Activity:

The ED50 as measured by the dose-dependent stimulation of human TF-1 cells is ≤ 0.2 ng/ml. This corresponds to a specific activity of ≥ 5 x 10＾6 units/mg.

Formulation:

Lyophilized from a 0.2µm concentrated (1mg/ml) solution in PBS pH 7.4.

Storage:

Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18 centigrade. Upon reconstitution IL4 should be stored at 4 centigrade between 2-7 days and for future use below -18 centigrade.

Drug name:

Binetrakin; Pitrakinra

Applications:

Interleukin-4 is used as a biological response modifier to boost the immune system in cancer therapy.

Examples of Clinical Use:

Boost the immune system in cancer therapy

Affected organisms:

Humans

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Human Interleukin 4 (IL-4) - Therapeutic Proteins

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Specification Drug Information Related Products Product Name: Human Interleukin 4 (IL-4) Description: Interleukin-4 is a pleiotropic cytokine produced primarily by activated T lymphocytes, basophils and mast cells. Introduction: Multiple immune response-modulating functions are performed by IL-4 on a variety of cell types and it has an important role in the regulator of isotype switching, induction of IgE production in B lymphocytes and differentiation of precursor T helper cells.

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| TNF-alpha protein (Val 77-Leu 233) is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

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Tumor Necrosis Factor alpha (TNF-alpha)

Specification

Drug Information

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Product Name:

Tumor Necrosis Factor alpha (TNF-alpha)

Description:

TNF-alpha protein (Val 77-Leu 233) is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

Introduction:

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD12a; p55/6) and TNF-R2 (TNF receptor type 2; CD12b; p75/8), bind to TNF-alpha.

Molecular Weight:

The recombinant human TNF-α consists of 158 amino acids and has a predicted molecular mass of 17.4 kDa which is also estimated by SDS-PAGE.

Synonyms:

DIF Protein; TNF-alpha Protein; TNFA Protein; TNFSF2 Protein;

Biological Activity:

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 5-50 pg/mL.

Purity:

>95 % as determined by SDS-PAGE

Formulation:

Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Storage:

Samples are stable for up to twelve months from date of receipt at -70 centigrade. Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Applications:

TNF-alpha is involved in fighting against the tumorigenesis.

Examples of Clinical Use:

Fighting against the tumorigenesis

Affected organisms:

Humans

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Tumor Necrosis Factor alpha (TNF-alpha) - Therapeutic Proteins

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TNF-alpha protein (Val 77-Leu 233) is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

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| Mullerian inhibiting substance (MIS), also named anti‑Mullerian hormone (AMH), is a tissue-specific TGF-beta superfamily growth factor. Its expression is restricted to the Sertoli cells of fetal and postnatal testis, and to the granulosa cells of postnatal ovary.

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Mullerian Inhibiting Substance (MIS)

Specification

Drug Information

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Product Name:

Mullerian Inhibiting Substance (MIS)

Description:

Mullerian inhibiting substance (MIS), also named anti‑Mullerian hormone (AMH), is a tissue-specific TGF-beta superfamily growth factor. Its expression is restricted to the Sertoli cells of fetal and postnatal testis, and to the granulosa cells of postnatal ovary.

Introduction:

The human MIS gene encodes a 553 amino acid residue (aa) prepropeptide containing a signal a sequence (1-24), a pro‑region (25-455), and the carboxyl-terminal bioactive protein (446-553) . MIS is synthesized and secreted as a disulfide-linked homodimeric pro‑protein. Proteolytic cleavage is required to generate the N-terminal pro‑region and the C‑terminal bioactive protein, which remain associated in a non-covalent complex. Recombinant C‑terminal MIS has been shown to be bioactive. However, the complex with the N-terminal pro‑region showed enhanced activity. The C‑terminal region contains the seven canonical cysteine residues found in TGF-beta superfamily members. These cysteine residues are involved in inter- and intra-molecular disulfide bonds, which forms the cysteine knot structure. Human and mouse MIS share 73% and 90% aa sequence identity in their pro‑region and C‑terminal region, respectively. MIS induces Mullerian duct (female reproductive tract) regression during sexual differentiation in the male embryo. Posnatally, MIS has been shown to regulate gonadal functions. MIS inhibits Leydig cell proliferation and is a regulator of the initial and cyclic recruitment of ovarian follicles. MIS has also been found to have anti‑proliferative effects on breast, ovarian and prostate tumor cells. Like other TGF-beta superfamily members, MIS signals via a heteromeric receptor complex consisting of a type I and a type II receptor serine/threonine kinase. Depending on the cell context, different type I receptors (including Act RIA/ALK2, BMP RIA/ALK3, and BMP RIB/ALK6) that are shared by other TGF-beta superfamily members, have been implicated in MIS signaling. In contrast, the type II MIS receptor (MIS RII) is unique and does not bind other TGF-beta superfamily members. Upon ligand binding, MIS RII recruits the non-ligand binding type I receptor into the complex, resulting in phosphorylation the BMP-like signaling pathway effector proteins Smad1, Smad5 and Smad 8.

Source:

E. coli-derived human MIS/AMH protein

Molecular Weight:

11.7 kDa (monomer)

Synonyms:

AMH; MIF; MIS; Muellerian hormone; muellerian-inhibiting factor; muellerian-inhibiting substance; Mullerian hormone; Mullerian inhibiting factor; Mullerian inhibiting substance;

Biological Activity:

Measured by its ability to inhibit growth of OVCAR‑3 human ovarian carcinoma cells. Measured by its binding ability in a functional ELISA. Immobilized rhMIS at 3 µg/mL (100 µL/well) will bind rrMIS RII/Fc Chimera with a linear range of 1.6-100 ng/mL.

Purity:

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Formulation:

Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.

Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution:

Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Endotoxin Level:

<0.01 EU per 1 μg of the protein by the LAL method.

Applications:

MIS has been shown to regulate gonadal functions. MIS inhibits Leydig cell proliferation and is a regulator of the initial and cyclic recruitment of ovarian follicles. MIS has also been found to have anti‑proliferative effects on breast, ovarian and prostate tumor cells.

Examples of Clinical Use:

Anti‑proliferative effects on breast, ovarian and prostate tumor cells

Affected organisms:

Humans

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Mullerian Inhibiting Substance (MIS) - Therapeutic Proteins

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Specification Drug Information Related Products Product Name: Mullerian Inhibiting Substance (MIS) Description: Mullerian inhibiting substance (MIS), also named anti‑Mullerian hormone (AMH), is a tissue-specific TGF-beta superfamily growth factor. Its expression is restricted to the Sertoli cells of fetal and postnatal testis, and to the granulosa cells of postnatal ovary.

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| Creative BioMart provides you with endotoxin-related Supporting reagents, in order to complete the detection and removal of endotoxins more accurately and without interference.
Mainly provide Tris HCl Buffer, MgCl2, Endotoxin-free Blood Anticoagulant，Glucan Blocker and Dispersing Agent.

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Creative BioMart provides you with endotoxin-related Supporting reagents, in order to complete the detection and removal of endotoxins more accurately and without interference.

Mainly provide Tris HCl Buffer, MgCl ₂, Endotoxin-free Blood Anticoagulant，Glucan Blocker and Dispersing Agent.

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Creative BioMart provides you with endotoxin-related Supporting reagents, in order to complete the detection and removal of endotoxins more accurately and without interference. Mainly provide Tris HCl Buffer, MgCl 2, Endotoxin-free Blood Anticoagulant，Glucan Blocker and Dispersing Agent.

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| Endotoxin (LPS) preparations are widely used to study the structure, metabolism, immunology, and biosynthesis of LPS. LPS is also used to induce the synthesis and secretion of growth-promoting factors such as interleukins. By combining LPS with FITC, TRITC, and TNP, it can be used to study intracellular responses such as immune response to LPS.

Creative BioMart offers a variety of LPS products, including a variety of bacterial sources, and various labeled LPS.

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Endotoxin (LPS) preparations are widely used to study the structure, metabolism, immunology, and biosynthesis of LPS. LPS is also used to induce the synthesis and secretion of growth-promoting factors such as interleukins. By combining LPS with FITC, TRITC, and TNP, it can be used to study intracellular responses such as immune response to LPS.

Creative BioMart offers a variety of LPS products, including a variety of bacterial sources, and various labeled LPS.

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Endotoxin (LPS) preparations are widely used to study the structure, metabolism, immunology, and biosynthesis of LPS. LPS is also used to induce the synthesis and secretion of growth-promoting factors such as interleukins. By combining LPS with FITC, TRITC, and TNP, it can be used to study intracellular responses such as immune response to LPS.

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| Creative BioMart offers a variety of endotoxin products, such as endotoxin testing kit, endotoxin removal kit, TAL/LAL reagent, endotoxin-free consumables, supporting reagent, LPS and derivatives. We are constantly expanding our product range and hope to further enhance our product portfolio and products in the coming year through further academic and industry cooperation.

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Home >> Products >> Endotoxin-free Purification Resins

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| Creative BioMart offers a variety of endotoxin products, such as endotoxin testing kit, endotoxin removal kit, TAL/LAL reagent, endotoxin-free consumables, supporting reagent, LPS and derivatives. We are constantly expanding our product range and hope to further enhance our product portfolio and products in the coming year through further academic and industry cooperation.

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Home >> Products >> Endotoxin-free Purification Kits

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| In endotoxin detection experiments, endotoxin removal experiments, and some experiments that require strict control of endotoxins, all experimental consumables required for the experiment should be made of products without endotoxin.

Creative BioMart provides you with a variety of endotoxin-free experimental supplies for bacterial and cell experiments.

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In endotoxin detection experiments, endotoxin removal experiments, and some experiments that require strict control of endotoxins, all experimental consumables required for the experiment should be made of products without endotoxin.

Creative BioMart provides you with a variety of endotoxin-free experimental supplies for bacterial and cell experiments.

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In endotoxin detection experiments, endotoxin removal experiments, and some experiments that require strict control of endotoxins, all experimental consumables required for the experiment should be made of products without endotoxin. Creative BioMart provides you with a variety of endotoxin-free experimental supplies for bacterial and cell experiments.

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| Creative BioMart offers you endotoxin removal kits. It is convenient for you to carry out the endotoxin removal work independently during the experiment and bring guarantee to your experiment.

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Creative BioMart offers you endotoxin removal kits. It is convenient for you to carry out the endotoxin removal work independently during the experiment and bring guarantee to your experiment.

Endotoxin removal kits are designed to efficiently remove endotoxin contamination from biological samples. It is suitable for the endotoxin removal of protein samples, monoclonal antibody drugs, therapeutic vaccines, small molecule chemical drugs, other proteins, peptides, antibodies, polysaccharides and other biological samples. The highly efficient endotoxin-specific adsorption substance is used as a ligand to specifically and efficiently remove endotoxin without affecting the activity of most biological samples.

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Creative BioMart offers you endotoxin removal kits. It is convenient for you to carry out the endotoxin removal work independently during the experiment and bring guarantee to your experiment. Endotoxin removal kits are designed to efficiently remove endotoxin contamination from biological samples.

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| Creative BioMart offers you a variety of Endotoxin Testing Kits, including multiple detection methods. You can choose according to your different needs for the experiment.

In addition to the detection reagents, the endotoxin detection kit also includes azo dyes, buffers, endotoxin standards, endotoxin-free test tubes and other related accessories. The endotoxin detection kit ensures that you can perform endotoxin detection experiments easily and quickly.

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Creative BioMart offers you a variety of Endotoxin Testing Kits, including multiple detection methods. You can choose according to your different needs for the experiment.

In addition to the detection reagents, the endotoxin detection kit also includes azo dyes, buffers, endotoxin standards, endotoxin-free test tubes and other related accessories. The endotoxin detection kit ensures that you can perform endotoxin detection experiments easily and quickly.

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Creative BioMart offers you a variety of Endotoxin Testing Kits, including multiple detection methods. You can choose according to your different needs for the experiment. In addition to the detection reagents, the endotoxin detection kit also includes azo dyes, buffers, endotoxin standards, endotoxin-free test tubes and other related accessories.

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| Creative BioMart offers a variety of endotoxin products, such as endotoxin testing kit, endotoxin removal kit, TAL/LAL reagent, endotoxin-free consumables, supporting reagent, LPS and derivatives. We are constantly expanding our product range and hope to further enhance our product portfolio and products in the coming year through further academic and industry cooperation.

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Creative BioMart provides customers with comprehensive endotoxin research services.Comprehensive,
one-stop service for a faster and more efficient experience.

Creative BioMart offers a variety of endotoxin-related products. Including endotoxin testing kits, endotoxin removal kits, endotoxin standards, purified LPS and its derivatives, endotoxin-free reagents, and consumables.

Creative BioMart provides customers with comprehensive endotoxin research services.Comprehensive,
one-stop service for a faster and more efficient experience.

Endotoxin Testing Services
Creative BioMart provides a full range of services for endotoxin testing. Our endotoxin testing services cover a wide range of methods and are available for a wide range of samples to provide comprehensive and efficient help for your experiments.

Endotoxin Removal Services
Creative BioMart provides a full range of services for endotoxin removal. Our endotoxin removal services cover a wide range of methods and are available for a wide range of samples to provide comprehensive and efficient help for your experiments.

Low Endotoxin Recovery
Low Endotoxin Recovery (LER) has a great impact on the detection of endotoxin, so to determine the accuracy of endotoxin detection, LER interference should be ruled out. Creative BioMart provides a hold-time studies to exclude LER interference services.

CONTACT US

USA

Tel: 1-631-559-9269 1-516-512-3133

Fax: 1-631-938-8127

Email: info@creative-biomart.com

45-1 Ramsey Road, Shirley, NY 11967, USA

Europe

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Creative BioMart provides customers with comprehensive endotoxin research services.Comprehensive, one-stop service for a faster and more efficient experience. Creative BioMart offers a variety of endotoxin-related products. Including endotoxin testing kits, endotoxin removal kits, endotoxin standards, purified LPS and its derivatives, endotoxin-free reagents, and consumables.

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| Interleukin-1 (IL-1) was originally thought to be a lymphocyte activating factor, but it was later found to have multiple effects, including inducing inflammation, raising body temperature, and stimulating T and B cell proliferation. Its activity is not limited to the immune system. IL-1 is also involved in regulating blood calcium levels, stimulating various body temperatures, regulating blood pressure or modulating sleeping. There is evidence that IL-1 is involved in the pathogenesis of certain cardiovascular diseases, and IL-1β can be used as a potential biomarker and therapeutic target for cardiovascular disease. Creative BioMartBiomarker is capable to provide our customers with superior IL-1β detection service, various detection methods ensuring high sensitivity for detecting biomarkers in different samples with different concentrations.

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Interleukin-1 (IL-1) was originally thought to be a lymphocyte activating factor, but it was later found to have multiple effects, including inducing inflammation, raising body temperature, and stimulating T and B cell proliferation. Its activity is not limited to the immune system. IL-1 is also involved in regulating blood calcium levels, stimulating various body temperatures, regulating blood pressure or modulating sleeping. There is evidence that IL-1 is involved in the pathogenesis of certain cardiovascular diseases, and IL-1β can be used as a potential biomarker and therapeutic target for cardiovascular disease. Creative BioMartBiomarker is capable to provide our customers with superior IL-1β detection service, various detection methods ensuring high sensitivity for detecting biomarkers in different samples with different concentrations.

Introduction

IL-1 is the term for two different but related proteins, namely IL-1α and IL-1β, encoded by two separate genes. They produce a 31 kDa precursor under stress or cell injury and are subject to proteolysis by specific proteases during maturation. IL-1α and IL-1β use the same membrane receptor (IL-1R) to trigger signal transduction pathways, leading to the activation of NF-κB or AP-1.

Figure 1. The IL-1 signaling and its negative regulation.

The lack of IL-1β reduces the severity of atherosclerosis in mice. The interaction of osteopontin and IL-1β may help the development of atherosclerotic lesions and the instability of atheromatous plaques. In addition, much evidence, including the human body, indicates that IL-1β plays an important role in insulin resistance in type 2 diabetes and pre-diabetic states, producing a large number of adipokines, including IL-1β. These adipokines can be released into the circulation system and affect distant organs, including the heart or vessels. Therefore, IL-1β plays an important role in cardiovascular disease. At present, there have been studies to use IL-1β as a target for cardiovascular disease therapy.

Application of IL-1β

Serum levels of IL-1β, can be used as a potential biomarker and therapeutic target for cardiovascular disease.

Our Advantages

Accept a wide range of sample types

Provide multiple detection methods

Ensure high sensitivity for detecting IL-1β in different samples

Ensure high accuracy and repeatable IL-1β detection

Ensure a wide kinetic range to detect samples of different concentrations

Short experimental period

Workflow of IL-1β Detection at Creative BioMart Biomarker

Creative BioMart Biomarker strictly controls each specific experimental step in the IL-1β detection procedure to ensure high sensitivity, high accuracy and repeatable IL-1β detection.

Please feel free to contact us if you would like to know more about IL-1β detection. At Creative BioMart Biomarker, we not only provide high-quality IL-1β detection service, but also provide detection services for other biomarkers. Additionally, our experts can also provide and help design the best solution according to your specific requirements.
References:

1. Vicenová, B.; et al. Emerging role of interleukin-1 in cardiovascular diseases. Physiol. Res. 2009, 58: 481-498.

2. Herder, C.; et al. Circulating levels of interleukin 1-receptor antagonist and risk of cardiovascular disease. Arterioscler Thromb Vasc Biol. 2017, 37: 1222-1227.

3. Peiró, C.; et al. IL-1β inhibition in cardiovascular complications associated to diabetes mellitus. Front Pharmacol. 2017, 8: 363.

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Interleukin-1 (IL-1) was originally thought to be a lymphocyte activating factor, but it was later found to have multiple effects, including inducing inflammation, raising body temperature, and stimulating T and B cell proliferation. Its activity is not limited to the immune system.

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| Interleukin 18 (IL-18) is a factor that plays a key role in inflammation and immune response. It is related to many autoimmune diseases and is one of the research targets of these diseases. Creative BioMart Biomarker provides scientists with fast and sensitive IL-18 detection services. The professional team and rich experience can provide customers with reliable detection results and help customers accelerate research progress.

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Interleukin 18 (IL-18) is a factor that plays a key role in inflammation and immune response. It is related to many autoimmune diseases and is one of the research targets of these diseases. Creative BioMart Biomarker provides scientists with fast and sensitive IL-18 detection services. The professional team and rich experience can provide customers with reliable detection results and help customers accelerate research progress.

IL-18

IL-18 is a protein expressed in many types of cells. It was originally discovered and described as an interferon-γ-inducing factor due to its important role in inflammation and immune response. IL-18 is a member of the IL-1 family. The IL-1 family initially contained only IL-1α and IL-1β, and later expanded to a family of 11 members (IL-1α, IL-1β, IL-1Ra, IL-18, IL-36α, IL-36β, IL-36Ra, IL-37, IL-36γ, IL-38, IL-33). There is a correlation between IL-18 and IL-1 β, they are first synthesized as inactive precursors, and both of them need caspase-1 and protease-3 for cleavage and extracellular processing, respectively. In addition, IL-18 and IL-1 α also have similarities, and their precursors are both present in mesenchymal cells and related to monocyte membranes. IL-18 works by binding to its receptor. The IL-18 receptor can bind to IL-37. The IL-18 precursor and IL-37 precursor have a closely related tertiary structure, and their gene structure also suggests the correlation between the two. IL-18 can induce T cells to produce IFN-γ, which requires the processing of caspase-1. The main immunomodulatory effect of IL-18 is reflected in NK cells. IL-18 is associated with a variety of diseases, including autoimmune diseases, metabolic syndrome, macrophage activation syndrome and sepsis, etc., and the production of IL-18 is increased in several autoimmune diseases. IL-18 may also be related to some clinical manifestations related to caspase-1 activation in inflammatory diseases. Therefore, IL-18 is currently an important target in the research of some immune diseases and inflammatory diseases.

Figure 1. IL-18 in Innate and Adaptive Immunity (Garlanda, et al. 2013)

Application of IL-18 Detection

IL-18 levels can be used as biomarkers in researches related to autoimmune diseases, metabolic syndrome, macrophage activation syndrome, sepsis, etc.

Our Advantages

Guarantee high accuracy and sensitivity for IL-18 detection

Ensure high repeatability of IL-18 detection

Experienced biomarker detection technical team

Strictly control each specific experimental step to ensure accurate and reliable test results in each sample

Short turnaround time to accelerate your research

More favorable prices in the biomarker testing service market can help reduce research costs

Detection Technologies

Samples

Sample types: serum, plasma, cell culture supernatants, etc.

Target species: human, mouse, rat, porcine, bovine, etc.

Workflow of Biomarker Detection at Creative BioMart Biomarker

At Creative BioMart Biomarker, we offer high-quality IL-18 detection service that ensuring the sensitivity and specificity of test results. You can also talk to our experts according to your certain requirement, and we will determine the final detection scheme based on the communication results. Please feel free to contact us, Creative BioMart Biomarker is here to offer you professional and thoughtful service.
References:

1. Garlanda, C.; et al. The interleukin-1 family: back to the future. Immunity. 2013, 39(6): 1003-1018.

2. Dinarello, C. A. Overview of the IL-1 family in innate inflammation and acquired immunity. Immunological Reviews. 2018, 281(1): 8-27.

# data[].attributes.attachment_link.description
Interleukin 18 (IL-18) is a factor that plays a key role in inflammation and immune response. It is related to many autoimmune diseases and is one of the research targets of these diseases. Creative BioMart Biomarker provides scientists with fast and sensitive IL-18 detection services.

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| Interleukin 17 (IL-17) is a key cytokine in the human body, and the signal transduction mechanism mediated by it is of great significance for the research of various immune diseases. Creative BioMart Biomarker provides testing services for IL-17A, IL-17B, IL-17F, etc. Our testing services are not suitable for private medical diagnosis and testing. Our efficient testing services with reasonable and competitive testing prices can help researchers shorten the research cycle and reduce research costs.

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Interleukin 17 (IL-17) is a key cytokine in the human body, and the signal transduction mechanism mediated by it is of great significance for the research of various immune diseases. Creative BioMart Biomarker provides testing services for IL-17A, IL-17B, IL-17F, etc. Our testing services are not suitable for private medical diagnosis and testing. Our efficient testing services with reasonable and competitive testing prices can help researchers shorten the research cycle and reduce research costs.

IL-17

IL-17 is a cytokine originally isolated from murine T-cell hybridomas. In the early stage, it was considered to be a subtype of the cytotoxic T lymphocyte-associated protein (CTLA) family, so it was called CTLA-8. It was later renamed IL-17. IL-17 has six homologous molecules, namely IL-17A, IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. Among them, IL-17A and IL-17F have a high degree of homology and have similar functions. IL-17A can be produced by several types of immune cells including T helper 17 cell. The receptor family of IL-17 has 5 members, including IL-17RA, IL-17RB, IL-17RC, IL-17RD and IL-17RE. The 5 receptors have sequence homology, and their shared structures include the fibronectin III-like domain in the extracellular region and the SEF/IL-17R (SEFIR) domain in the intracellular region. IL-17RA is the shared receptor of IL-17 family cytokines, and IL-17RB, IL-17RC and IL-17RE are the specific receptors of IL-17E, IL-17A, IL-17F and IL-17C, respectively. IL-17RD is involved in the regulation of IL-17A signal transduction. IL-17A and IL-17F can induce pro-inflammatory gene expression alone or in conjunction with TNFα, IL-6, G-CSF, IL-1, CXCL, CCL20 and matrix metalloproteinases. IL-17 can also upregulate pro-inflammatory genes by activating NF-κB, MAPK and C/EBP cascades. In addition, IL-17 can activate the Jak-Stat and Jak-PI3 K pathways. The protein Act1 is also closely related to the functional pathway of IL-17. Act1 can serve as an anchoring platform at the proximal end of the receptor to initiate a TRAF6-dependent signaling pathway and a TRAF2-TRAF5-dependent cascade activated by IL-17.

Figure 1. Interleukin-17 Receptor Signaling (Zenobia, et al. 2015)

Application of IL-17 Detection

IL-17 levels can be used as biomarkers in researches related to rheumatoid arthritis, cancer, multiple sclerosis, chronic inflammatory bowel disease, etc.

Our Advantages

Guarantee high accuracy and sensitivity for IL-17 detection

Ensure high repeatability of IL-17 detection

Experienced biomarker detection technical team

Strictly control each specific experimental step to ensure accurate and reliable test results in each sample

Short turnaround time to accelerate your research

More favorable prices in the biomarker testing service market can help reduce research costs

Detection Technologies

Samples

Sample types: serum, plasma, cell culture supernatants, etc.

Target species: human, mouse, rabbit, rat, porcine, canine, monkey, etc.

Workflow of Biomarker Detection at Creative BioMart Biomarker

At Creative BioMart Biomarker, we offer high-quality IL-17 detection service that ensuring the sensitivity and specificity of test results. You can also talk to our experts according to your certain requirement, and we will determine the final detection scheme based on the communication results. Please feel free to contact us, Creative BioMart Biomarker is here to offer you professional and thoughtful service.
References:

1. Kuwabara, T.; et al. The role of IL-17 and related cytokines in inflammatory autoimmune diseases. Mediators of Inflammation. 2017, 2017: 3908061.

2. Zenobia, C.; Hajishengallis, G. Basic biology and role of interleukin‐17 in immunity and inflammation. Periodontology. 2015, 69(1): 142-159.

# data[].attributes.attachment_link.description
Interleukin 17 (IL-17) is a key cytokine in the human body, and the signal transduction mechanism mediated by it is of great significance for the research of various immune diseases. Creative BioMart Biomarker provides testing services for IL-17A, IL-17B, IL-17F, etc. Our testing services are not suitable for private medical diagnosis and testing.

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| Interleukin-10 (IL-10) is a pleiotropic cytokine with important immune regulation functions, and is often an important target in the researches of immune diseases and immune responses. As a leader in biomarker services, Creative BioMart Biomarker provides efficient, accurate and reliable IL-10 testing services for scientists both in academia and industry, and has been committed to helping customers shorten research cycles and reduce research costs.

# data[].attributes.attachment_link.readable_text
Interleukin-10 (IL-10) is a pleiotropic cytokine with important immune regulation functions, and is often an important target in the researches of immune diseases and immune responses. As a leader in biomarker services, Creative BioMart Biomarker provides efficient, accurate and reliable IL-10 testing services for scientists both in academia and industry, and has been committed to helping customers shorten research cycles and reduce research costs.

IL-10

IL-10 is also known as human cytokine synthesis inhibitory factor (CSIF), which is a pleiotropic, immunoregulatory cytokine encoded by the IL10 gene. IL-10 is mainly produced by B cells, macrophages, dendritic cells (DC), CD4⁺ and CD8⁺ T cells. It is a homodimer formed by the combination of two subunits each composed of 178 amino acids. During infection, IL-10 can affect the activity of many types of cells in the immune system. IL-10 can mediate its effects on T cells and natural killer (NK) cells functions through its direct effects on monocytes and macrophages. In addition, IL-10 can reduce the expression of MHC class II molecules on the surface of DCs and macrophages, weakening the function of antigen-presenting cells (APCs). It can also reduce the expression of proinflammatory cytokines and chemokines, thereby reducing the inflammatory response. IL-10 in CD4⁺ T cells can inhibit the production and proliferation of cytokines such as IFN-γ and TNF-α, so it can directly regulate Th1 and Th2 responses by inhibiting T cell activity and differentiation. IL-10 participates in the regulation of JAK-STAT signal transduction pathway through its receptor IL-10 receptor-1 and IL-10 receptor-2, thereby exerting its biological functions. IL-10 can protect the host from infection-related immunopathology. In patients with autoimmune diseases such as systemic lupus erythematosus (SLE) and multiple sclerosis (MS), IL-10 has abnormal serum levels, and the imbalance of IL-10 expression in patients may play an important role in these diseases.

Figure 1. The sources and targets of IL-10 during infection (Couper, et al. 2008)

Application of IL-10 Detection

IL-10 levels can be used as biomarkers in researches related to SLE, MS, etc.

Our Advantages

Guarantee high accuracy and sensitivity for IL-10 detection

Ensure high repeatability of IL-10 detection

Experienced biomarker detection technical team

Strictly control each specific experimental step to ensure accurate and reliable test results in each sample

Short turnaround time to accelerate your research

More favorable prices in the biomarker testing service market can help reduce research costs

Detection Technologies

Samples

Sample types: serum, plasma, cell culture supernatants, etc.

Target species: human, mouse, rabbit, rat, bovine, canine, chicken, etc.

Workflow of Biomarker Detection at Creative BioMart Biomarker

At Creative BioMart Biomarker, we offer high-quality IL-10 detection service that ensuring the sensitivity and specificity of test results. You can also talk to our experts according to your certain requirement, and we will determine the final detection scheme based on the communication results. Please feel free to contact us, Creative BioMart Biomarker is here to offer you professional and thoughtful service.
References:

1. Couper, K. N.; et al. IL-10: the master regulator of immunity to infection. The Journal of Immunology. 2008, 180(9): 5771-5777.

2. Ng, T. H. S.; et al. Regulation of adaptive immunity; the role of interleukin-10. Frontiers in Immunology. 2013, 4: 129.

# data[].attributes.attachment_link.description
Interleukin-10 (IL-10) is a pleiotropic cytokine with important immune regulation functions, and is often an important target in the researches of immune diseases and immune responses. As a leader in biomarker services, Creative BioMart Biomarker provides efficient, accurate and reliable IL-10 testing services for scientists both in academia and industry, and has been committed to helping customers shorten research cycles and reduce research costs.

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Insulin like growth factor binding protein 5 test

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Insulin like growth factor binding protein 5 test

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| Insulin like growth factor binding protein 5 (IGFBP5) is a biomarker with clinical application prospects related to various tumors. As an expert in the field of biomarkers, Creative BioMart Biomarker has an experienced technical team and a mature cancer biomarker detection platform, which can provide customers with fast, accurate and sensitive IGFBP5 test results, helping customers shorten the research cycle and reduce research costs.

# data[].attributes.attachment_link.readable_text
Insulin like growth factor binding protein 5 (IGFBP5) is a biomarker with clinical application prospects related to various tumors. As an expert in the field of biomarkers, Creative BioMart Biomarker has an experienced technical team and a mature cancer biomarker detection platform, which can provide customers with fast, accurate and sensitive IGFBP5 test results, helping customers shorten the research cycle and reduce research costs.

IGFBP5 and Cancers

IGFBP is a key regulator of insulin-like growth factors (IGFs) and contains six protein families (IGFBP1, IGFBP2, IGFBP3, IGFBP4, IGFBP5, IGFBP6), and IGFBP5 is one of them that have the relatively more conserved building blocks. IGFs are a class of growth factors with mitogenic and metabolic activities, which can activate the tyrosine kinase pathway by binding to the receptors on the cell surface. IGFBP5 regulates the function of IGF by binding to the conserved domain of IGF. IGFBP5 is localized in the cytoplasm and nucleus, and its combination with IGF may inhibit physiological activities such as proliferation and differentiation. The IGF system participates in many important functions in the human body, including proliferation, differentiation, tumor migration, and invasion. It can be used in clinical diagnosis and disease pathological development monitoring, such as cancer progression and endocrine responsiveness. IGF is up-regulated during tumorigenesis, and IGFBP5 is thought to regulate tumor proliferation and spread, reflecting the development and metastasis of cancer. Therefore, IGFBP5 is considered to be a biomarker for clinical treatment responses and clinical outcomes of various tumors, such as breast cancer, prostate cancer, lung cancer, bladder cancer, thyroid cancer, cervical cancer, salivary gland cancer, etc. IGFBP5 is differentially expressed in cancer tissues, which may be related to the degree of cell differentiation in different cancers. At the same time, the increase of IGFBP5 level is also related to tumor metastasis and migration, so IGFBP5 can be used in tumor research to indicate whether the tumor has metastasized or migrated. IGFBP5 is related to the prognosis of various cancers, and can be used as a prognostic biomarker for breast cancer, ovarian cancer, glioblastoma, etc., indicating clinical results, response to treatment, and survival rate. IGFBP5 is a promising biomarker in various tumor researches, and also has development potential in the development of cancer drugs.

Figure 1. Schematic view of IGFBP5 and possible regulation in cell proliferation networks (Güllü, et al. 2012)

Application of IGFBP5 Detection

Plasma and serum IGFBP5 levels as biomarkers to predict different diseases, such as breast cancer, prostate cancer, lung cancer, bladder cancer, thyroid cancer, etc.

Our Advantages

Guarantee high accuracy and sensitivity for IGFBP5 detection

Ensure high repeatability of IGFBP5 detection

Short turn-around time of detection service

Competitive price in the market of IGFBP5 detection services

Provide multiple IGFBP5 detection methods, including ELISA, colorimetric assay, etc.

Accept a wide range of sample types (plasma, serum and cell culture supernates)

Workflow of Biomarker Detection at Creative BioMart Biomarker

Creative BioMart Biomarker strictly controls each specific experimental step in the detection procedure to ensure accurately quantify the level of IGFBP5 in each sample.

At Creative BioMart Biomarker, we offer IGFBP5 detection service that refer to different methods, you can communicate with our experts according to your research needs, and we will determine the final detection technological scheme based on the communication results. Please feel free to contact us, Creative BioMart Biomarker is here to offer you professional and thoughtful service.
References:

1. Murukesh, N.; et al. Biomarkers of angiogenesis and their role in the development of VEGF inhibitors. British Journal of Cancer. 2010, 102(1): 8-18.

2. Macedo, F.; et al. Gastric cancer and angiogenesis: is VEGF a useful biomarker to assess progression and remission? Journal of Gastric Cancer. 2017, 17(1): 1-10.

3. Alsohaimi, A. The pathogenic role of vascular endothelial growth factor (VEGF) in skin diseases. Advances in Medicine and Medical Research. 2019, 2(1): 27-37.

4. Güllü, G.; et al. Functional roles and clinical values of insulin-like growth factor-binding protein-5 in different types of cancers. Chinese Journal of Cancer. 2012, 31(6): 266-280.

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Insulin like growth factor binding protein 5 (IGFBP5) is a biomarker with clinical application prospects related to various tumors. As an expert in the field of biomarkers, Creative BioMart Biomarker has an experienced technical team and a mature cancer biomarker detection platform, which can provide customers with fast, accurate and sensitive IGFBP5 test results, helping customers shorten the research cycle and reduce research costs.

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| Interferon (IFN)-γ is one of the most studied immune biomarkers. Creative BioMart Biomarker provides sensitive and reliable IFN-γ detection services for customers in related research fields. We have many years of experience in biomarker testing services, and efficient testing and accurate results can effectively help customers advance research progress.

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Interferon (IFN)-γ is one of the most studied immune biomarkers. Creative BioMart Biomarker provides sensitive and reliable IFN-γ detection services for customers in related research fields. We have many years of experience in biomarker testing services, and efficient testing and accurate results can effectively help customers advance research progress.

IFN-γ

IFNs are pleiotropic cytokines with anti-tumor and immunomodulatory properties. IFNs can be classified according to genetic loci, sequence identity, cell of origin, distribution of receptors, etc. IFN-α and IFN-β constitute the human type I IFN family, and IFN-γ is the only member of the type II IFN family. IFN-γ is mainly secreted by activated lymphocytes, but also by B cells, natural killer (NK) T cells and professional antigen-presenting cells. It is a homodimer formed by the non-covalent bonding of two polypeptide subunits, and has undergone multiple N-glycosylation during the synthesis process, and finally formed a 50kDa molecule. The biological effects of IFN-γ are achieved by activating molecular signaling pathways in cells. The combination of IFN-γ and its receptors IFNγR1 and IFNγR2 can activate the JAN/STAT pathway, thereby regulating the transcription of hundreds of genes and mediating different biological responses. The pleiotropic functions of IFN-γ are mediated by apoptosis and cell cycle regulators, inflammatory signaling molecules, transcriptional activators, etc. IFN-γ mainly activates anti-cancer immunity by promoting the activity of immune-related cells (including macrophages, NK cells, dendritic cells, etc.). It can also inhibit the differentiation and function of regulatory T cells, Th2 and Th17. IFN-γ is a major participant in human systemic autoimmune responses, and abnormal expression of IFN-γ can be found in autoimmune diseases. At the same time, its cellular targets may have the application prospect of prediction and treatment in tumor.

Figure 1. Immunomodulatory effects of IFN-γ (Castro, et al. 2018)

Application of IFN-γ Detection

IFN-γ levels can be used as biomarkers in researches related to rheumatoid arthritis, systemic lupus erythematous, etc.

Our Advantages

Guarantee high accuracy and sensitivity for IFN-γ detection

Ensure high repeatability of IFN-γ detection

Experienced biomarker detection technical team

Strictly control each specific experimental step to ensure accurate and reliable test results in each sample

Short turnaround time to accelerate your research

More favorable prices in the biomarker testing service market can help reduce research costs

Detection Technologies

Samples

Sample types: serum, plasma, tissue homogenates, cell culture supernatants, etc.

Target species: human, mouse, rabbit, rat, bovine, canine, chicken, goat, etc.

Workflow of Biomarker Detection at Creative BioMart Biomarker

At Creative BioMart Biomarker, we offer high-quality IFN-γ detection service that ensuring the sensitivity and specificity of test results. You can also talk to our experts according to your certain requirement, and we will determine the final detection scheme based on the communication results. Please feel free to contact us, Creative BioMart Biomarker is here to offer you professional and thoughtful service.
References:

1. Castro, F.; et al. Interferon-gamma at the crossroads of tumor immune surveillance or evasion. Frontiers in Immunology. 2018, 9: 847.

2. Pollard, K. M.; et al. Interferon-γ and systemic autoimmunity. Discovery Medicine. 2013, 16(87): 123-131.

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Interferon (IFN)-γ is one of the most studied immune biomarkers. Creative BioMart Biomarker provides sensitive and reliable IFN-γ detection services for customers in related research fields. We have many years of experience in biomarker testing services, and efficient testing and accurate results can effectively help customers advance research progress.

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| Intercellular adhesion molecule-1 (ICAM-1) is an immune-related molecule, which has research value in a variety of immune-related diseases. Creative BioMart Biomarker provides customers with ICAM-1 ELISA and CLIA testing services. Our testing services are suitable for many different types of samples and can meet the needs of different researchers. Our ICAM-1 testing service is not suitable for private medical diagnosis and testing.

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Intercellular adhesion molecule-1 (ICAM-1) is an immune-related molecule, which has research value in a variety of immune-related diseases. Creative BioMart Biomarker provides customers with ICAM-1 ELISA and CLIA testing services. Our testing services are suitable for many different types of samples and can meet the needs of different researchers. Our ICAM-1 testing service is not suitable for private medical diagnosis and testing.

Intercellular Adhesion Molecule-1

ICAM-1 is a transmembrane glycoprotein with a size of about 100kDa and belongs to the immunoglobulin supergene family. ICAM-1 is expressed in leukocyte subsets, endothelial cells, fibroblasts, glial cells, epithelial cells, etc. The full-length isoform of ICAM-1 consists of five Ig domains, a transmembrane domain, and a short cytoplasmic tail containing multiple threonine residues, which can interact with actin cytoskeleton and the ezrin/radixin/moesin complex associated with the lipid rafts. Membrane-bound ICAM-1 plays an important role in lymphocyte activation, leukocyte trafficking and some other immune functions. ICAM-1 can bind to a variety of ligands, including LFA-1, Mac-1, fibrinogen, hyaluronan and major rhinoviruses. The circulating form of ICAM-1 in body fluids, namely soluble ICAM-1 (sICAM-1), is produced by the proteolytic cleavage and alternative splicing of membrane-bound ICAM-1. sICAM-1 can also interact with LFA-1 and Mac-1. sICAM-1 is often studied as a biomarker and modulator that can promote inflammation. Its serum level is generally low, but it will increase in many inflammation-related pathological processes. Therefore, in a variety of diseases, ICAM-1 can be considered as a biomarker related to disease progression and severity.

Figure 1. Schematic representation of ICAM-1 isoforms. (A) Full-length ICAM-1 isoform. (B) ICAM-1 full-length isoforms dimerize by interaction through the fourth Ig domains. (C) The six membrane ICAM-1 isoforms. (D) Under inflammatory conditions, the Ig domain 5 of the full-length isoform is truncated.

Application of ICAM-1 Detection

ICAM-1 levels can be used as biomarkers in researches related to cancer, atherosclerosis, bacterial sepsis, type 2 diabetes, etc.

Our Advantages

Guarantee high accuracy and sensitivity for ICAM-1 detection

Ensure high repeatability of ICAM-1 detection

Experienced biomarker detection technical team

Strictly control each specific experimental step to ensure accurate and reliable test results in each sample

Short turnaround time to accelerate your research

More favorable prices in the biomarker testing service market can help reduce research costs

Detection Technologies

Samples

Sample types: serum, plasma, cell culture supernatants, tissue homogenates, etc.

Target species: human, mouse, rabbit, rat, canine, etc.

Workflow of Biomarker Detection at Creative BioMart Biomarker

At Creative BioMart Biomarker, we offer high-quality ICAM-1 detection service that ensuring the sensitivity and specificity of test results. You can also talk to our experts according to your certain requirement, and we will determine the final detection scheme based on the communication results. Please feel free to contact us, Creative BioMart Biomarker is here to offer you professional and thoughtful service.
References:

1. Ramos, T.N.; et al. ICAM-1: isoforms and phenotypes. The Journal of Immunology. 2014, 192 (10): 4469-4474.

2. Müller, N. The role of intercellular adhesion molecule-1 in the pathogenesis of psychiatric disorders. Front Pharmacol. 2019, 10: 1251.

# data[].attributes.attachment_link.description
Intercellular adhesion molecule-1 (ICAM-1) is an immune-related molecule, which has research value in a variety of immune-related diseases. Creative BioMart Biomarker provides customers with ICAM-1 ELISA and CLIA testing services. Our testing services are suitable for many different types of samples and can meet the needs of different researchers.

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| Hyaluronic acid (HA) is a ubiquitous molecule with a wide range of physiological functions. It is an intrinsic molecule in the knee joint and can be used as a biomarker for indicating osteoarthritis. In addition, HA can be used as an indicator of disease progression. Creative BioMart Biomarker offers high quality detection service for HA, ensuring high detection accuracy, sensitivity and efficiency for each sample.

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Hyaluronic acid (HA) is a ubiquitous molecule with a wide range of physiological functions. It is an intrinsic molecule in the knee joint and can be used as a biomarker for indicating osteoarthritis. In addition, HA can be used as an indicator of disease progression. Creative BioMart Biomarker offers high quality detection service for HA, ensuring high detection accuracy, sensitivity and efficiency for each sample.

Introduction

HA is also known as hyaluronan, which is almost ubiquitous in the body. HA is the simplest glycosaminoglycan (GAG), and also the only GAG that is not synthesized in the Golgi apparatus. The HA solution always exhibits a high viscosity. It is a water soluble polymer with special enzymatic degradation. The synthesis of HA does not need to be attached to the core protein. It is the main component of the extracellular matrix (ECM) and is present in some soft connective tissues, including synovial fluid, ocular vitreous, cartilage and skin. Combined with its rheological properties, HA has a protective effect on cartilage, which helps to explain the long-term beneficial effects of HA on articular cartilage. HA exists in two forms based on the chain length, namely low molecular weight HA chain (LMWHA) and high molecular weight HA chain (HMWHA). The functions of cell communication, movement and morphogenesis are due to the interaction of transparent plasmids with tissue receptors, mainly CD44 and RHAMM on the cell surface. Reduce inflammation and apoptosis by down-regulating many of the factors that cause ECM. HMWHA inhibits phagocytosis, macrophage activation, and production of inflammatory cytokines. The HMWHA chain can be broken down into LMWHA, which has pro-inflammatory effects. These fragments secrete inflammatory cytokines and stimulate angiogenesis and tissue remodeling after activation of the endogenous signaling pathway. They promote the activation and maturation of dendritic cells and release pro-inflammatory cytokines. Molecular changes in the damaged joint ECM alter the composition and structure of the native HA. With the secretion of molecules and tissue remodeling, pathological development has also taken place. As OA progresses, the natural HA concentration and the distribution of intra-articular HA change, resulting in a decrease in the mechanical/viscoelastic properties of the endogenous synovial fluid. The abundance and size distribution of HA in biological fluids is considered to be an indicator of inflammation.

Application of HA Detection

Serum and plasma HA levels as biomarkers to predict bone diseases, such as osteoarthritis.

Our Advantages

Guarantee high accuracy and sensitivity for HA detection

Ensure high repeatability of HA detection

Short turn-around time of detection service

Competitive price in the market of detection services

Accept a wide range of sample types (serum, plasma, etc.)

Detection Technologies

Workflow of HA Detection at Creative BioMart Biomarker

Creative BioMart Biomarker strictly controls each specific experimental step in the HA detection procedure to ensure accurately quantify the level of HA in each sample.

At Creative BioMart Biomarker, we not only provide high-quality HA detection service, but also provide detection services for other biomarkers. You can communicate with our experts according to your research needs, and we will determine the final detection technological scheme based on the communication results. Please feel free to contact us, Creative BioMart Biomarker is here to offer you professional and thoughtful service.
References:

1. Terpos, E.; et al. The role of markers of bone remodeling in multiple myeloma. Blood Reviews. 2005, 19(3): 125-142.

2. Eastell, R.; Szulc, P. Use of bone turnover markers in postmenopausal osteoporosis. The Lancet Diabetes & Endocrinology. 2017, 11(5): 908-923.

3. Unnanuntana, A.; et al. The assessment of fracture risk. Journal of Bone & Joint Surgery American Volume. 2010, 92(3): 743-753.

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Hyaluronic acid (HA) is a ubiquitous molecule with a wide range of physiological functions. It is an intrinsic molecule in the knee joint and can be used as a biomarker for indicating osteoarthritis. In addition, HA can be used as an indicator of disease progression.

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| Creative BioMart specializes in providing cost-effective assay kits and custom services to both academia and biotech/pharmaceutical industries in the study of enzyme activity, protein biology, and small molecule detection.

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Enzyme Activity Assays | Creative BioMart - Assay Kit

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| Creative BioMart specializes in providing cost-effective assay kits and custom services to both academia and biotech/pharmaceutical industries in the study of enzyme activity, protein biology, and small molecule detection.

We have developed and manufactured over thousands of enzyme activity assay kits. They are designed to analyze almost every class of enzymes, including oxidoreductases, transferases, hydrolases, lyases, isomerases, and ligases. They are also available in different detection methods. All of our assay kits are ready to use, and they are all provided with necessary reagents, instructions, and analysis protocols to help researchers quickly obtain accurate and reproducible results.

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About Creative BioMart Assay Kit

Creative BioMart specializes in providing cost-effective assay kits and custom services to both academia and biotech/pharmaceutical industries in the study of enzyme activity, protein biology, and small molecule detection.

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Creative BioMart specializes in providing cost-effective assay kits and custom services to both academia and biotech/pharmaceutical industries in the study of enzyme activity, protein biology, and small molecule detection.

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| Creative BioMart is experienced on assay kit validation and has gained great reputation in market. We are glad to help you ensure the quality of commercial assay kits, thus allowing smooth experiments. If you are looking for assay kit validation solutions, please contact us for custom service.

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Performance of assays kits purchased from manufacturers is highly recommended to be validated before use. Variations caused by lot-lot difference might lead to inconsistent assay results, which could be avoid beforehand by kit validation. Some manufacturers might implement rigorous quality control, while some might not. Necessity of validating the performance of assay kits is therefore apparent. Creative BioMart provides validation service to check the quality of your assay kits with regards to specificity, sensitivity, reproducibility, and linearity.

Assay Kit Validation Workflow

We are able to carry out validation with various types of assay kits, including ELISA kit, enzyme activity assay kit, quantification kit, sample preparation kit, et al. Positive control and negative control samples will be purchased if they are available in market. Otherwise customers are required to provide them for validation. Statistical analysis is presented in the validation report to conclude the performance of your assay kits.

Creative BioMart is experienced on assay kit validation and has gained great reputation in market. We are glad to help you ensure the quality of commercial assay kits, thus allowing smooth experiments. If you are looking for assay kit validation solutions, please contact us for custom service.

Inquiry

We are here to answer any question you may have

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Kit Validation | Creative BioMart - Assay Kit

# data[].attributes.attachment_link.description
Performance of assays kits purchased from manufacturers is highly recommended to be validated before use. Variations caused by lot-lot difference might lead to inconsistent assay results, which could be avoid beforehand by kit validation. Some manufacturers might implement rigorous quality control, while some might not.

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| Commercial assay kits are extensively used by scientists and researchers in a broad range of areas, such as biological research, drug development, medical test, food quality control, et al. We are devoted to development of novel assay kits that fill up the blank in market or that serve our valued customers. If the assay kit you are looking for is not available on market yet, please feel free to send a development request to Creative BioMart – Assay Kit. We are ready to help you design and produce your own kits as per your requirement.

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Commercial assay kits are extensively used by scientists and researchers in a broad range of areas, such as biological research, drug development, medical test, food quality control, et al. We are devoted to development of novel assay kits that fill up the blank in market or that serve our valued customers. If the assay kit you are looking for is not available on market yet, please feel free to send a development request to Creative BioMart - Assay Kit. We are ready to help you design and produce your own kits as per your requirement.

Assay Kit Development Workflow

Key Features

We provide custom assay kit service to cover activity determination of different types of enzymes, including but not limited to oxidoreductases, transferases, hydrolases, lyases, isomerases, and ligases. Metabolites and hormones are associated with a number of urgent health issues. Detection of theses biomarkers therefore enables research on metabolic syndrome diagnosis. We comply comprehensive strategies to fulfill requirements of accurate and precise assay of biomarkers of interest. Moreover, assay kits which are used to detect cellular components, biochemical processes, epigenetic modifications, and cell signaling pathways, can be developed and produced by our professional team as well.

Creative BioMart has grown to the global leader in protein and enzyme sicence since last decade. Our collaborators in research institutes, industries, and governments think highly of our top services and products. Our mission is to make your assay quick, easy to handle, cost-effective, accurate, and reproducible. Please contact us if you plan to creative your own assay kit.

Inquiry

We are here to answer any question you may have

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Kit Development | Creative BioMart - Assay Kit

# data[].attributes.attachment_link.description
Commercial assay kits are extensively used by scientists and researchers in a broad range of areas, such as biological research, drug development, medical test, food quality control, et al. We are devoted to development of novel assay kits that fill up the blank in market or that serve our valued customers.

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| Ingredients and additives in food and cosmetics constantly draw public attention due to their potential impact on human health. Quality control plays a critical role before food product and cosmetics become available in the market. Creative BioMart endeavors to provide food and cosmetics related substances testing service using our assay kits to ensure test reliability, sensitivity, and specificity.

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Ingredients and additives in food and cosmetics constantly draw public attention due to their potential impact on human health. Quality control plays a critical role before food product and cosmetics become available in the market. Creative BioMart endeavors to provide food and cosmetics related substances testing service using our assay kits to ensure test reliability, sensitivity, and specificity.

Our tests by assay kit cover a wide range of substances present or potentially present in food and cosmetics. Generally, your food product sample is processed and subject to quantification of acidity, hydrogen peroxide, peroxide value, carbonyl value, iodine value, soap content, unsaponifiable matter, pesticide residue, sibutramine et al. We also provide quick detection of contaminant microorganisms found in food product, such as Aspergillus, Penicillium, E. coli, et al. It is also available to test cosmetics sample concerning D-sorbitol, nitroimidazole, minoxidil, hydroquinone, sulfonamides, formaldehyde, boric acid, lincomycin, et al. As heavy metal accumulation prevalent in plants and animals, it comes imperative to assure the safe level of them in food and cosmetics. We are experienced in quick test of calcium, copper, iron, magnesium, cadmium, mercury, aluminum, nickel, cobalt, zinc, lead, tin, manganese, chromium, selenium, et al.

Creative BioMart is proud to serve you with sensitive and reliable food and cosmetics related substances testing in a short turnaround time. Please feel free to contact us if you plan to have your sample tested.

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Food and Cosmetics Related Substances Test | Creative BioMart - Assay Kit

# data[].attributes.attachment_link.description
Ingredients and additives in food and cosmetics constantly draw public attention due to their potential impact on human health. Quality control plays a critical role before food product and cosmetics become available in the market. Creative BioMart endeavors to provide food and cosmetics related substances testing service using our assay kits to ensure test reliability, sensitivity, and specificity.

# data[].attributes.body
| Creative BioMart specializes in providing cost-effective assay kits and custom services to both academia and biotech/pharmaceutical industries in the study of enzyme activity, protein biology, and small molecule detection.

We have developed and manufactured over thousands of enzyme activity assay kits. They are designed to analyze almost every class of enzymes, including oxidoreductases, transferases, hydrolases, lyases, isomerases, and ligases. They are also available in different detection methods. All of our assay kits are ready to use, and they are all provided with necessary reagents, instructions, and analysis protocols to help researchers quickly obtain accurate and reproducible results.

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Detection Assays | Creative BioMart - Assay Kit

# data[].attributes.body
| Creative BioMart specializes in providing cost-effective assay kits and custom services to both academia and biotech/pharmaceutical industries in the study of enzyme activity, protein biology, and small molecule detection.

We have developed and manufactured over thousands of enzyme activity assay kits. They are designed to analyze almost every class of enzymes, including oxidoreductases, transferases, hydrolases, lyases, isomerases, and ligases. They are also available in different detection methods. All of our assay kits are ready to use, and they are all provided with necessary reagents, instructions, and analysis protocols to help researchers quickly obtain accurate and reproducible results.

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Cellular and Biochemical Assays | Creative BioMart - Assay Kit

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Metabolism and Endocrinology Assays | Creative BioMart - Assay Kit

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Recombinant urate-oxidase enzyme

Specification

Drug Information

Related Products

Product Name:

Recombinant urate-oxidase enzyme

Description:

The product is a recombinant urate-oxidase enzyme produced by a genetically modified Saccharomyces cerevisiae strain. The cDNA coding was cloned from a strain of Aspergillus flavus.

Formula:

C1521H2381N417O461S7

Sequences:

SAVKAARYGKDNVRVYKVHKDEKTGVQTVYEMTVCVLLEGEIETSYTKADNSVIVATDSIKNTIYITAKQNPVTPPELFGSILGTHFIEKYNHIHAAHVNIVCHRWTRMDIDGKPHPHSFIRDSEEKRNVQVDVVEGKGIDIKSSLSGLTVLKSTNSQFWGFLRDEYTTLKETWDRILSTDVDATWQWKNFSGLQEVRSHVPKFDATWATAREVTLKTFAEDNSASVQATMYKMAEQILARQQLIETVEYSLPNKHYFEIDLSWHKGLQNTGKNAEVFAPQSDPNGLIKCTVGRSSLKSKL

Species:

Aspergillus flavus

Source:

Saccharomyces cerevisiae

Molecular Weight:

34109.5 Da

Synonyms:

Urate oxidase; Uricase

Drug name:

Rasburicase

Applications:

For treatment of hyperuricemia, reduces elevated plasma uric acid levels (from chemotherapy).

Examples of Clinical Use:

Hyperuricemia

Pharmacodynamics :

Drugs used to treat lympohoid leukemia, non-Hodgkin's lymphoma and acute myelogenous leukemia often lead to the accumulation of toxic plasma levels of purine metabolites (i.e. uric acid). The injection of the product reduces levels of uric acid and mitigates the toxic effects of chemotherapy induced tumor lysis.

Mechanism of action:

Rasburicase catalyzes enzymatic oxidation of uric acid into an inactive and soluble metabolite (allantoin).

Affected organisms:

Humans and other mammals

Targets:

Target 1. Uric acid

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Recombinant urate-oxidase enzyme - Therapeutic Proteins

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Specification Drug Information Related Products Product Name: Recombinant urate-oxidase enzyme Description: The product is a recombinant urate-oxidase enzyme produced by a genetically modified Saccharomyces cerevisiae strain. The cDNA coding was cloned from a strain of Aspergillus flavus.

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Recombinant interleukin eleven

Specification

Drug Information

Related Products

Product Name:

Recombinant interleukin eleven

Description:

The product, the active ingredient is recombinant Interleukin eleven, which is produced in Escherichia coli (E. coli) by recombinant DNA technology. The protein has a molecular mass of approximately 19,000 daltons, and is non-glycosylated. The polypeptide is 177 amino acids in length (the natural IL-11 has 178).

Formula:

C854H1411N253O235S2

Sequences:

GPPPGPPRVSPDPRAELDSTVLLTRSLLADTRQLAAQLRDKFPADGDHNLDSLPTLAMSAGALGALQLPGVLTRLRADLLSYLRHVQWLRRAGGSSLKTLEPELGTLQARLDRLLRRLQLLMSRLALPQPPPDPPAPPLAPPSSAWGGIRAAHAILGGLHLTLDWAVRGLLLLKTRL

Molecular Weight:

19047.2 Da

Synonyms:

IL-11; Interleukin 11; Interleukin-11; Oprelvekin; Recombinant interleukin-11

Applications:

Increases reduced platelet levels due to chemotherapy

Examples of Clinical Use:

Reduced platelet levels

Pharmacodynamics :

The product is indicated for the prevention of severe thrombocytopenia and the reduction of the need for platelet transfusions following myelosuppressive chemotherapy in adult patients with nonmyeloid malignancies who are at high risk of severe thrombocytopenia. The primary hematopoietic activity of the product is stimulation of megakaryocytopoiesis and thrombopoiesis. The product has shown potent thrombopoietic activity in individuals with compromised hematopoiesis

Mechanism of action:

Oprelvekin binds to the interleukin 11 receptor which leads to a cascade of signal transduction events. IL-11 is a thrombopoietic growth factor that directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production.

Affected organisms:

Humans and other mammals

Targets:

Target 1. Interleukin-11 receptor subunit alpha

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Recombinant interleukin eleven - Therapeutic Proteins

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The product, the active ingredient is recombinant Interleukin eleven, which is produced in Escherichia coli (E. coli) by recombinant DNA technology. The protein has a molecular mass of approximately 19,000 daltons, and is non-glycosylated. The polypeptide is 177 amino acids in length (the natural IL-11 has 178).

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B domain deleted recombinant factor VIII (BDDrFVIII)

Specification

Drug Information

Related Products

Product Name:

B domain deleted recombinant factor VIII (BDDrFVIII)

Description:

The product, also known as BDDrFVIII (B domain deleted recombinant factor VIII), is a recombinant DNA-based product with functional characteristics comparable to those of endogenous coagulation Factor VIII, the essential human blood clotting protein that is impaired in Hemophilia A. The product is identical in sequence to endogenously produced Factor VIII, but does not contain the B-domain, which has no known biological function. The product is produced through recombinant DNA technology and purification, resulting in a 1438 amino acid, 170 kDa protein. Clinical evaluation has shown that BDDrFVIII is pharmacokinetically equivalent to full-length recombinant FVIII.

Formula:

Not Available

Sequences:

Not Available

Molecular Weight:

173000.0 Da (glycosylated)

Synonyms:

Antihemophilic factor (recombinant, B-domain deleted), plasma/albumin free; Antihemophilic factor recombinant plasma/albumin free; Antihemphilic factor, recombinant human B-domain deleted; B-domain deleted recombinant factor VIII; BDDrFVIII; Human factor VIII, recombinant B-domain deleted

Drug name:

Moroctocog alfa

Applications:

The product is approved by Health Canada for the control and prevention of hemorrhagic episodes and for routine and surgical prophylaxis in patients with hemophilia A (congenital factor VIII deficiency or classic hemophilia). The product is also approved by the European Medicines Agency for the treatment and prophylaxis of bleeding in patients with Haemophilia A (congenital factor VIII deficiency).

Examples of Clinical Use:

Bleeding in Haemophilia A (congenital factor VIII deficiency).

Pharmacodynamics :

Antihemophilic Factor binds factor IXa along with calcium and phospholipid, which converts factor X to factor Xa to facilitate the clotting cascade.

Mechanism of action:

Antihemophilic factor (AHF) is a protein found in normal plasma which is necessary for clot formation. The administration of AHF provides an increase in plasma levels of AHF and can temporarily correct the coagulation defect of patients with hemophilia A (classical hemophilia). As factor VIII is the specific clotting factor deficient in patients with hemophilia A, replacement of clotting factor with Moroctocog alfa, also known as BDDrFVIII (B domain deleted recombinant factor VIII), is the cornerstone of the prevention and treatment of bleeding for this disorder.

Affected organisms:

Not Available

Targets:

Target 1. Coagulation factor X; Target 2. Phytanoyl-CoA dioxygenase, peroxisomal; Target 3. Coagulation factor IX; Target 4. Asialoglycoprotein receptor 2; Target 5. 78 kDa glucose-regulated protein; Target 6. Calreticulin; Target 7. Calnexin; Target 8. Protein ERGIC-53; Target 9. Prolow-density lipoprotein receptor-related protein 1; Target 10. Multiple coagulation factor deficiency protein 2; Target 11. von Willebrand factor; Enzyme 1. Prothrombin; Enzyme 2. Vitamin K-dependent protein C

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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B domain deleted recombinant factor VIII (BDDrFVIII) - Therapeutic Proteins

# data[].attributes.attachment_link.description
The product, also known as BDDrFVIII (B domain deleted recombinant factor VIII), is a recombinant DNA-based product with functional characteristics comparable to those of endogenous coagulation Factor VIII, the essential human blood clotting protein that is impaired in Hemophilia A.

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Erythropoiesis stimulating agent (ESA)

Specification

Drug Information

Related Products

Product Name:

Erythropoiesis stimulating agent (ESA)

Description:

The product is a chemically synthesised Erythropoiesis Stimulating Agent (ESA) with a longer half-life than erythropoietin.

Formula:

Not Available

Sequences:

Not Available

Molecular Weight:

Not Available

Synonyms:

CERA; Pegzerepoetin alfa

Drug name:

Methoxy polyethylene glycol-epoetin beta

Applications:

For the treatment of patients with anaemia associated with chronic kidney disease. Not a substitute for RBC transfusion if immediate correction of anemia is required.

Examples of Clinical Use:

Anaemia associated with chronic kidney disease

Pharmacodynamics :

Stimulates hemoglobin production by stimulating the erythropoetin receptor of erythroid progenitor cells in the bone marrow. Hemoglobin increase, following a single initial dose, occurs 7 to 15 days after.

Mechanism of action:

Erythropoietin is a growth factor for erythroid development. It is produced in the kidney and released into the bloodstream in response to hypoxia, interacting with erythroid progenitor cells to increase red blood cell production. Production of endogenous erythropoietin is impaired in patients with chronic kidney disease (CKD), and erythropoietin deficiency is the primary cause of their anaemia. Administration of methoxy polyethylene glycol-epoetin beta acts like endogenous erythropoetin and stimulates erythropoetin receptor of the erythroid progenitor cells in the bone marrow.

Affected organisms:

Not Available

Targets:

Target 1. Erythropoietin receptor

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Erythropoiesis stimulating agent (ESA) - Therapeutic Proteins

# data[].attributes.attachment_link.description
Specification Drug Information Related Products Product Name: Erythropoiesis stimulating agent (ESA) Description: The product is a chemically synthesised Erythropoiesis Stimulating Agent (ESA) with a longer half-life than erythropoietin. Formula: Not Available Sequences: Not Available Molecular Weight: Not Available Synonyms: CERA; Pegzerepoetin alfa Drug name: Methoxy polyethylene glycol-epoetin beta Applications: For the treatment of patients with anaemia associated with chronic kidney disease.

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Pegylated, recombinant granulocyte colony-stimulating factor (G-CSF)

Specification

Drug Information

Related Products

Product Name:

Pegylated, recombinant granulocyte colony-stimulating factor (G-CSF)

Description:

The product is a pegylated, recombinant granulocyte colony-stimulating factor (G-CSF) that was synthetized using a highly site-specific glycoPEGylation technology. The product is a covalent conjugate of Filgrastim with a single methoxy polyethylene glycol (PEG) molecule via a carbohydrate linker consisting of glycine, N-acetylneuraminic acid and N-acetylgalactosamine. The average molecular mass of the product comprises 18,798 Da for Filgrastim, 203 Da for GalNAc, 338 Da for glycylsialic acid and approximately 20,000 Da for PEG.

Formula:

C866H1372N226O258S9*(C2H4O)n

Sequences:

MTPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEELVLLGHSLGIPWAPLSSCPSQALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTLQLDVADFATTIWQQMEELGMAPALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRVLRHLAQP

Molecular Weight:

39000.0 Da (glycoPEGylated, approximate)

Synonyms:

Not Available

Drug name:

Lipegfilgrastim

Applications:

Indicated for the reduction in the duration of neutropenia and the incidence of febrile neutropenia in adult patients treated with cytotoxic chemotherapy for malignancy (with the exception of chronic myeloid leukaemia and myelodysplastic syndromes).

Examples of Clinical Use:

Reduction in the duration of neutropenia and the incidence of febrile neutropenia

Pharmacodynamics :

Mimicking endogenous granulocyte colony-stimulating factors, the product enhances the number and function of circulating neutrophils by binding to endogenous G-CSF receptors. A small increase in monocyte and/or lymphocyte counts may also be observed. Following a single subcutaneous dose administration of 100 μg/kg, the product resulted in a significant increase in neutrophilic granulocyte and large unstained cell counts. G-CSF also increases the antibacterial activities of neutrophils including the phagocytosis. Due to structural similarity between the product and pegfilgrastim, G-CSF receptor binding was equivalent between two molecules. However, the product showed greater time-dependent resistance to neutrophil elastase degradation and greater retention of activity than pegfilgrastim.

Mechanism of action:

Endogenous granulocyte colony-stimulating factor (G-CSF) is a glycoprotein that stimulates neutrophil progenitors. It is produced mainly by monocytes, fibroblasts and endothelial cells to promote the development of neutrophils and increase their proliferation and maturation. Subsequently, G-SCF stimulates the release of matured neutrophils from the bone marrow storage pools into the peripheral blood to enhance their function. Via binding to to the human G-CSF receptors, lipegfilgrastim activates the receptor signalling pathway as a growth factor to stimulate proliferation of haematopoietic progenitor cells and their differentiation into mature cells, and promote subsequent release into the peripheral blood. This stimulatory effect of lipegfilgrastim may extend to other single lineage and multilineage progenitors and pluripotent haematopoietic stem cells. The presence of the PEG moiety in lipegfilgrastim decreases the plasma clearance and extends the drug's terminal elimination half-life, allowing for less frequent dosing.

Affected organisms:

Not Available

Targets:

Target 1. Granulocyte colony-stimulating factor receptor

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Pegylated, recombinant granulocyte colony-stimulating factor (G-CSF) - Therapeutic Proteins

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The product is a pegylated, recombinant granulocyte colony-stimulating factor (G-CSF) that was synthetized using a highly site-specific glycoPEGylation technology. The product is a covalent conjugate of Filgrastim with a single methoxy polyethylene glycol (PEG) molecule via a carbohydrate linker consisting of glycine, N-acetylneuraminic acid and N-acetylgalactosamine.

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Natural hirudin (leucine for isoleucine at the N-terminal end of the molecule and the absence of a sulfate group on the tyrosine at position 63)

Specification

Drug Information

Related Products

Product Name:

Natural hirudin (leucine for isoleucine at the N-terminal end of the molecule and the absence of a sulfate group on the tyrosine at position 63)

Description:

The product is identical to natural hirudin except for substitution of leucine for isoleucine at the N-terminal end of the molecule and the absence of a sulfate group on the tyrosine at position 63. It is produced via yeast cells.

Formula:

C287H440N80O110S6

Sequences:

LVYTDCTESGQNLCLCEGSNVCGQGNKCILGSDGEKNQCVTGEGTPKPQSHNDGDFEEIPEEYLQ

Molecular Weight:

6963.425 Da

Synonyms:

Hirudin variant-1; Lepirudin recombinant

Applications:

For the treatment of heparin-induced thrombocytopenia.

Examples of Clinical Use:

Heparin-induced thrombocytopenia

Pharmacodynamics :

The product is used to break up clots and to reduce thrombocytopenia. It binds to thrombin and prevents thrombus or clot formation. It is a highly potent, selective, and essentially irreversible inhibitor of thrombin and clot-bond thrombin. The product requires no cofactor for its anticoagulant action. The product is a recombinant form of hirudin, an endogenous anticoagulant found in medicinal leeches.

Mechanism of action:

Lepirudin forms a stable non-covalent complex with alpha-thrombin, thereby abolishing its ability to cleave fibrinogen and initiate the clotting cascade. The inhibition of thrombin prevents the blood clotting cascade.

Affected organisms:

Humans and other mammals

Targets:

Target 1. Prothrombin

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Natural hirudin (leucine for isoleucine at the N-terminal end of the molecule and the absence of a sulfate group on the tyrosine at position 63) - Therapeutic Proteins

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The product is identical to natural hirudin except for substitution of leucine for isoleucine at the N-terminal end of the molecule and the absence of a sulfate group on the tyrosine at position 63. It is produced via yeast cells.

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Recombinant granulocyte colony-stimulating factor

Specification

Drug Information

Related Products

Product Name:

Recombinant granulocyte colony-stimulating factor

Description:

The product is a recombinant granulocyte colony-stimulating factor which functions as an immunostimulator.

Formula:

Not Available

Sequences:

Not Available

Molecular Weight:

Not Available

Synonyms:

G-CSF (CHO cell derived); Glycosylated recombinant G-CSF; Glycosylated recombinant granulocyte colony stimulating factor; Granulocyte colony stimulating factor 3 (CHO cell derived); Granulocyte colony-stimulating factor lenograstim; Lenograstim (genetical recombination); Lenograstim rDNA

Drug name:

Lenograstim

Applications:

The drug is used to reduce the risk of life-threatening infection in patients with neutropenia, particularly after cytotoxic chemotherapy. The product is indicated as a treatment to reduce the duration of neutropenia and the severity of infections in patients with non-myeloid malignancy who have undergone autologous or allogeneic bone marrow transplantation, or treatment with established cytotoxic chemotherapy and in addition to reduce the incidence of infection associated with established cytotoxic chemotherapy. The product is also indicated to mobilise peripheral blood progenitor cells (PBPCs) with The product alone, or after myelosuppressive chemotherapy, in order to accelerate haematopoietic recovery by infusion of such cells, after myelosuppressive or myeloablative therapy. The product is also indicated to accelerate the engraftment of these cells after their reinfusion. The product is also indicated for the treatment of severe chronic neutropenia including congenital agranulocytosis (Kostmann's syndrome).

Examples of Clinical Use:

Severe chronic neutropenia including congenital agranulocytosis (Kostmann's syndrome).

Pharmacodynamics :

The product has been confirmed as a valuable adjunct to minimise the haematological toxicity of myelosuppressive chemotherapy in patients with malignant disease. The drug also enhances neutrophil recovery in patients undergoing stem cell rescue, and assists peripheral blood progenitor cells mobilisation.

Mechanism of action:

Lenograstim is the glycosylated recombinant form of human granulocyte colony stimulating factor. Lenograstim accelerates neutrophil recovery significantly after chemotherapy, with beneficial effects on clinical end-points such as incidence of laboratory-confirmed infection and length of hospital stay. Chemotherapy dose intensity has also been increased in patients receiving lenograstim, notably those with breast or small cell lung cancer, although improvements in tumour response and survival have not been demonstrated. Lenograstim also assists neutrophil recovery in patients undergoing bone marrow transplantation, and stimulates the production of peripheral blood stem cells (PBSCs) for autologous transfusion after aggressive chemotherapy.

Affected organisms:

Not Available

Targets:

Target 1. Granulocyte colony-stimulating factor receptor

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Recombinant granulocyte colony-stimulating factor - Therapeutic Proteins

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Specification Drug Information Related Products Product Name: Recombinant granulocyte colony-stimulating factor Description: The product is a recombinant granulocyte colony-stimulating factor which functions as an immunostimulator.

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Recombinant non-naturally occurring type-I interferon

Specification

Drug Information

Related Products

Product Name:

Recombinant non-naturally occurring type-I interferon

Description:

The product is a recombinant non-naturally occurring type-I interferon. The 166-amino acid sequence of The product was derived by scanning the sequences of several natural interferon alpha subtypes and assigning the most frequently observed amino acid in each corresponding position. Four additional amino acid changes were made to facilitate the molecular construction, and a corresponding synthetic DNA sequence was constructed using chemical synthesis methodology. The product differs from interferon alfa-2b at 20/166 amino acids (88% homology), and comparison with interferon-beta shows identity at over 30% of the amino acid positions. The product is produced in Escherichia coli (E. coli) cells that have been genetically altered by insertion of a synthetically constructed sequence that codes for the product. Prior to final purification, the product is allowed to oxidize to its native state, and its final purity is achieved by sequential passage over a series of chromatography columns. This protein has a molecular weight of 19,434 daltons.

Formula:

C860H1353N227O255S9

Sequences:

MCDLPQTHSLGNRRALILLAQMRRISPFSCLKDRHDFGFPQEEFDGNQFQKAQAISVLHEMIQQTFNLFSTKDSSAAWDESLLEKFYTELYQQLNDLEACVIQEVGVEETPLMNVDSILAVKKYFQRITLYLTEKKYSPCAWEVVRAEIMRSFSLSTNLQERLRRKE

Molecular Weight:

19343.0 Da

Synonyms:

IFN Alfacon-1; IFN-Con1; Interferon alfacon-1

Drug name:

Interferon alfacon-1

Applications:

For the treatment of hairy cell leukemia, malignant melanoma, and AIDS-related Kaposi's sarcoma.

Examples of Clinical Use:

Hairy cell leukemia, malignant melanoma, and AIDS-related Kaposi's sarcoma

Pharmacodynamics :

Upregulates the expression of MHC I proteins, allowing for increased presentation of peptides derived from viral antigens. This enhances the activation of CD8+ T cells that are the precursors for cytotoxic T lymphocytes (CTLs) and makes the macrophage a better target for CTL-mediated killing. Interferon alpha also induce the synthesis of several key antiviral mediators, including 2'-5' oligoadenylate synthetase (2'-5' A synthetase) and protein kinase R.

Mechanism of action:

Interferon alpha binds to type I interferon receptors (IFNAR1 and IFNAR2c) which, upon dimerization, activate two Jak (Janus kinase) tyrosine kinases (Jak1 and Tyk2). These transphosphorylate themselves and phosphorylate the receptors. The phosphorylated INFAR receptors then bind to Stat1 and Stat2 (signal transducers and activators of transcription) which dimerize and activate multiple (~100) immunomodulatory and antiviral proteins. Interferon alpha binds less stably to type I interferon receptors than interferon beta. The resulting actions include gene transcription, inhibition of cellular growth, alteration of the state of cellular differentiation, interference with oncogene expression, alteration of cell surface antigen expression, increasing phagocytic activity of macrophages, and augmentation of the cytotoxicity of lymphocytes for target cells.

Affected organisms:

Humans and other mammals

Targets:

Target 1. Interferon alpha/beta receptor 1; Target 2. Interferon alpha/beta receptor 2

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Recombinant non-naturally occurring type-I interferon - Therapeutic Proteins

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The product is a recombinant non-naturally occurring type-I interferon. The 166-amino acid sequence of The product was derived by scanning the sequences of several natural interferon alpha subtypes and assigning the most frequently observed amino acid in each corresponding position.

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Creative BioMart offers you TAL / LAL Reagent for endotoxin detection experiments. TAL / LAL Reagent can be used as a supplement to the detection reagents in the Endotoxin Testing Kit. It can also be purchased separately and used in conjunction with buffers and reaction tubes.

TAL / LAL reagents provided by Creative BioMart include a variety of specifications: vials, ampoules, vacuum test tubes, etc. In addition, TAL / LAL reagent also includes a variety of detection methods, such as Gel Clot LAL Assay, Chromogenic LAL Assay and Chromogenic LAL Assay.

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Creative BioMart offers you TAL / LAL Reagent for endotoxin detection experiments. TAL / LAL Reagent can be used as a supplement to the detection reagents in the Endotoxin Testing Kit. It can also be purchased separately and used in conjunction with buffers and reaction tubes.

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Creative BioMart provides you with endotoxin-related Supporting reagents, in order to complete the detection and removal of endotoxins more accurately and without interference.

Mainly provide Tris HCl Buffer, MgCl ₂, Endotoxin-free Blood Anticoagulant，Glucan Blocker and Dispersing Agent.

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Creative BioMart provides you with endotoxin-related Supporting reagents, in order to complete the detection and removal of endotoxins more accurately and without interference. Mainly provide Tris HCl Buffer, MgCl 2, Endotoxin-free Blood Anticoagulant，Glucan Blocker and Dispersing Agent.

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Endotoxin (LPS) preparations are widely used to study the structure, metabolism, immunology, and biosynthesis of LPS. LPS is also used to induce the synthesis and secretion of growth-promoting factors such as interleukins. By combining LPS with FITC, TRITC, and TNP, it can be used to study intracellular responses such as immune response to LPS.

Creative BioMart offers a variety of LPS products, including a variety of bacterial sources, and various labeled LPS.

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Endotoxin (LPS) preparations are widely used to study the structure, metabolism, immunology, and biosynthesis of LPS. LPS is also used to induce the synthesis and secretion of growth-promoting factors such as interleukins. By combining LPS with FITC, TRITC, and TNP, it can be used to study intracellular responses such as immune response to LPS.

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Home >> Products >> Endotoxin-free Purification Resins

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Home >> Products >> Endotoxin-free Purification Kits

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In endotoxin detection experiments, endotoxin removal experiments, and some experiments that require strict control of endotoxins, all experimental consumables required for the experiment should be made of products without endotoxin.

Creative BioMart provides you with a variety of endotoxin-free experimental supplies for bacterial and cell experiments.

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In endotoxin detection experiments, endotoxin removal experiments, and some experiments that require strict control of endotoxins, all experimental consumables required for the experiment should be made of products without endotoxin. Creative BioMart provides you with a variety of endotoxin-free experimental supplies for bacterial and cell experiments.

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Creative BioMart offers you endotoxin removal kits. It is convenient for you to carry out the endotoxin removal work independently during the experiment and bring guarantee to your experiment.

Endotoxin removal kits are designed to efficiently remove endotoxin contamination from biological samples. It is suitable for the endotoxin removal of protein samples, monoclonal antibody drugs, therapeutic vaccines, small molecule chemical drugs, other proteins, peptides, antibodies, polysaccharides and other biological samples. The highly efficient endotoxin-specific adsorption substance is used as a ligand to specifically and efficiently remove endotoxin without affecting the activity of most biological samples.

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Creative BioMart offers you endotoxin removal kits. It is convenient for you to carry out the endotoxin removal work independently during the experiment and bring guarantee to your experiment. Endotoxin removal kits are designed to efficiently remove endotoxin contamination from biological samples.

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Creative BioMart offers you a variety of Endotoxin Testing Kits, including multiple detection methods. You can choose according to your different needs for the experiment.

In addition to the detection reagents, the endotoxin detection kit also includes azo dyes, buffers, endotoxin standards, endotoxin-free test tubes and other related accessories. The endotoxin detection kit ensures that you can perform endotoxin detection experiments easily and quickly.

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Creative BioMart offers you a variety of Endotoxin Testing Kits, including multiple detection methods. You can choose according to your different needs for the experiment. In addition to the detection reagents, the endotoxin detection kit also includes azo dyes, buffers, endotoxin standards, endotoxin-free test tubes and other related accessories.

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| https://biomarker.creativebiomart.net/dkk-1-detection-service.html

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Dickkopf-1 (DKK-1) is an inhibitor of Wnt signaling pathway involved in the regulation of bone resorption, DKK-1 is associated with a variety of bone diseases such as rheumatoid arthritis, multiple myeloma, Paget's disease and osteoporosis. Creative BioMart Biomarker offers high quality detection service for DKK-1, ensuring high detection accuracy, sensitivity and efficiency for each sample.

Introduction

DKK-1 is a secreted protein with two cysteine rich regions and belongs to the DKK family. DKK family is mainly composed of DKK-1, DKK-2, DKK-3, DKK-4 and a DKK-3 related protein which is known as soggy. They can participate in physiological activities by inhibiting Wnt signaling pathway. Among them, DKK-1 is the better-studied member that was originally found in the Xenopus, it is widely expressed in osteoblasts, osteocytes, skin, prostate, placenta, etc. Wnt signaling pathway plays an important role in cell fate determination, cell proliferation, cell polarity, cell migration, embryonic development, stem cell maintenance and tissue repair in the case of injury. In general, the Wnt signal is initiated by the binding of Wnt proteins to Frizzled (Fzd) receptors which can bind to a coreceptor such as lipoprotein receptor related protein 5 and 6 (LRP5/6), to form a complex that activates the typical and atypical Wnt signaling pathways. Only when bind to Fzd and LRP5/6 simultaneously to form a receptor-ligand complex, can Wnt protein stimulate the Wnt signaling pathway. Wnt1 protein binds to β-propeller1 of LRP5/6, and Wnt3 binds to β-propeller3 of LRP5/6, while DKK-1 not only binds to β-propeller1 of LRP5/6, but also binds to β-propeller3. Since the interaction between Wnt protein and β-propeller is blocked, Wnt signaling pathway is suppressed. In addition, DKK-1 can also enhance its inhibitory function by using coreceptors. Wnt signaling pathway is an important regulator of bone development and bone balance, and it increases bone mass through a number of mechanisms, including stem cell turnover, stimulation of pre-osteoblastic replication, induction of osteoblastogenesis, and inhibition of apoptosis for osteoblasts and osteocytes. DKK-1 disrupts cascade reaction of Wnt signaling to inhibit osteoblast differentiation. Decreased expression of DKK-1 leads to an increase in Wnt activity and bone mass, while increased expression of DKK-1 may result in an increase in osteoblast-dependent osteoclastogenesis. Studies have shown that circulating DKK-1 levels are sufficient to reflect its expression in the bone microenvironment, therefore, DKK-1 is an important biomarker of bone resorption.

Figure 1. The prevailing model of the role of DKK-1 and the mesenchymal cell lineage in bone development and bone mass homeostasis (Pinzone, et al. 2008)

Application of DKK-1 Detection

Serum DKK-1 level as a biomarker to predict different diseases, such as osteoporosis, rheumatoid arthritis, Paget's disease, multiple myeloma, etc.

Our Advantages

Guarantee high accuracy and sensitivity for DKK-1 detection

Ensure high repeatability of DKK-1 detection

Short turn-around time of detection service

Competitive price in the market of detection services

Provide multiple DKK-1 detection methods, including ELISA and EIA

Workflow of DKK-1 Detection at Creative BioMart Biomarker

Creative BioMart Biomarker strictly controls each specific experimental step in the DKK-1 detection procedure to ensure accurately quantify the level of DKK-1 in each sample.

At Creative BioMart Biomarker, we offer DKK-1 detection service that includes several technical methods, you can communicate with our experts according to your research needs, and we will determine the final detection technological scheme based on the communication results. Please feel free to contact us, Creative BioMart Biomarker is here to offer you professional and thoughtful service.
References:

1. Pinzone, J.J.; et al. The role of dickkopf-1 in bone development, homeostasis, and disease. Blood. 2008, 113(3): 517-525.

2. Huang, Y.; et al. Dickkopf-1: Current knowledge and related diseases. Life Sciences. 2018, 209: 249-254.

3. Terpos, E.; et al. Biology and treatment of myeloma related bone disease. Metabolism. 2017, 80: 80-90.

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Dickkopf-1 (DKK-1) is an inhibitor of Wnt signaling pathway involved in the regulation of bone resorption, DKK-1 is associated with a variety of bone diseases such as rheumatoid arthritis, multiple myeloma, Paget's disease and osteoporosis. Creative BioMart Biomarker offers high quality detection service for DKK-1, ensuring high detection accuracy, sensitivity and efficiency for each sample.

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Differential screening is an important part of biomarker research. Rigorous screening experiment design and sensitive sample analysis can help screen out biomarkers that are conducive to clinical transformation. Due to the complex composition of biological samples, the initial screening of biomarkers is a difficult part. As an expert in the field of biomarker services, Creative BioMart Biomarker has an experienced expert team and technical team, as well as a variety of advanced technical platforms that can be used for differential screening of biomarkers. We can help customers to customize the differential screening scheme according to the research needs of customers.

Biomarkers can specifically and sensitively reflect the state of the disease, and are used in the early screening, diagnosis, course monitoring, staging, prognosis and treatment response evaluation of various diseases. For clinical applications, ideal biomarkers can be clearly related to specific diseases or disease states, and can be used to distinguish similar physiological conditions. At the same time, the method to obtain such biomarkers should be convenient, from standard biological sources, such as serum and urine. In addition, ideal biomarkers can be quickly, easily, and accurately detected by existing detection technologies. Although some biomarkers have been found to be used as indicators of diseases or disease states, there is still a need to find new biomarkers to further promote the sensitivity and specificity of disease screening, thereby promoting the early diagnosis of diseases. Differential screening is an important means to find new biomarkers, usually through biotechnology analysis of differential expression between normal control samples and experimental samples to achieve the purpose of biomarker screening. With the advancement of biotechnology, not only can traditional biotechnologies be used for differential screening of biomarkers, the development of omics technology has also brought a broader development space for the discovery of new biomarkers. For example, differential expression analysis of serum glycoproteins by proteomics technology is used to screen new biomarkers for hepatocellular carcinoma. Omics technology has the advantages of high throughput and automation, enabling multiple markers to be screened at one time. The advancement of science has brought a variety of different analysis technologies to the field of biomarkers, which can provide different information for differential screening, and increase the chance of discovering new biomarkers, as well as help biomarkers move to clinical applications faster.

Figure 1. Workflow for urinary protein biomarker discovery (Duangkumpha, et a l. 2019)

Optional Specimen Type

Serum, plasma, urine, CSF, whole blood, platelets, PBMC, cell lysates, tissue culture media, cultured cells, biopsy.

Optional Sample Category

DNA, RNA, protein/polypeptide, glycan, hormone

Optional Screening Methods

Protein Microarrays

Antibody Microarrays

Two-Dimensional Electrophoresis

All-Liquid Separations

Mass Spectrometry

Tissue Microarrays

Magnetic Resonance Imaging (MRI)

Our Advantages

Multiple biomarker analysis technology platforms

Optional high-throughput screening platform

Experienced technical team

Strict and standard experimental operation

Provide complete experimental data and detailed professional analysis reports

High quality and more efficient custom services

Short service cycle and more competitive prices

Workflow of Custom Biomarker Service at Creative BioMart Biomarker

At Creative BioMart Biomarker, we provide customers with high-quality custom biomarker service related to differential screening, and our expert team can customize scientific and rigorous experimental programs as soon as possible according to customer research needs and sample types. Our technical team has many years of experience in biomarker analysis and has been strictly controlling each process in the screening to ensure that customers are provided with accurate, precise, and repeatable screening results. If you are seeking related services, Creative BioMart Biomarker may be your ideal choice, please feel free to contact us for more information.
References:

1. Duangkumpha, K.; et al. Urine proteomics study reveals potential biomarkers for the differential diagnosis of cholangiocarcinoma and periductal fibrosis. PLOS One. 2019,14(8): e0221024.

2. Gao, H. J.; et al. Quantitative proteomic analysis for high-throughput screening of differential glycoproteins in hepatocellular carcinoma serum. Ann Transl Med. 2015, 12(3): 246-254.

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Differential screening is an important part of biomarker research. Rigorous screening experiment design and sensitive sample analysis can help screen out biomarkers that are conducive to clinical transformation. Due to the complex composition of biological samples, the initial screening of biomarkers is a difficult part.

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CXCL12 is a CXC chemokine that contains multiple alternative splicing forms in humans. CXCL12 is involved in a variety of physiological and pathological processes, including embryogenesis, hematopoiesis, angiogenesis, and inflammatory responses, as well as cardiovascular disease (CVD). Creative BioMart Biomarker is capable to provide our customers with superior CXCL12 detection service, various detection methods ensuring high sensitivity for detecting biomarkers in different samples with different concentrations.

Introduction

The CXC chemokine CXCL12, also known as stromal cell-derived factor 1 (SDF-1), is one of the 17 members of the CXC chemokine family. CXCL12 is an important factor in physiological and pathological processes including embryogenesis, hematopoiesis, angiogenesis and inflammatory responses as it activates or induces migration of hematopoietic progenitor and stem cells, endothelial cells and most leukocytes. Therefore, the activity of CXCL12 is strictly regulated at multiple levels. CXCL12 has six splice variants in humans, each having a specific tissue distribution and in vivo activity. Controlled splice variant transcription and mRNA stability determine the CXCL12 expression profile. CXCL12 interacts with its receptor CXC chemokine receptor 4 (CXCR4) and atypical chemokine receptor 3 (ACKR3) and binds to glycosaminoglycans (GAGs) in tissues and on the endothelium, function under pathological and pathological conditions. The CXCL12/CXCR4 axis plays an important role in progenitor cell migration. Mobilization of hematopoietic stem cells, progenitor cells such as endothelial progenitor cells (EPC) and smooth muscle progenitor cells (SPC), are associated with a variety of diseases such as atherosclerosis. Studies have shown that the CXCL12/CXCR4 axis has anti-atherosclerotic effects. In addition to its effect on progenitor cells to mediate beneficial effects on atherosclerosis, CXCL12/CXCR4 can also affect disease progression by affecting a variety of cells associated with atherosclerosis.

Figure 1. Signal transduction pathways activated by CXCL12. (Janssens, R. 2018)

Genome-wide studies have shown a clear association between CXCL12 and CVD. Previous studies have shown that high levels of CXCL12 are expressed in vascular cells of human atherosclerotic lesions, but not in normal blood vessels. Studies in various humans have shown that CXCL12 is a potential regulator of atherosclerosis. A study comparing the levels of plasma CXCL12 in healthy controls with angina patients revealed a decrease in CXCL12 levels in the patient group. Patients with unstable angina have lower levels of CXCL12 than patients with stable angina. Taken together, this study demonstrates that CXCL12 has anti-atherosclerotic properties. In another study, platelet CXCL12 expression was significantly increased in patients with stable angina compared with healthy controls, indicating that CXCL12 has stronger atherogenic and pro-thrombotic. Therefore, CXCL12 can be used as an early biomarker for CVD.

Application of CXCL12

Plasma, serum levels of CXCL12, can be used as a predictor of cardiovascular disease.

Our Advantages

Accept a wide range of sample types

Provide multiple detection methods

Ensure high sensitivity for detecting CXCL12 in different samples

Ensure high accuracy and repeatable CXCL12 detection

Ensure a wide kinetic range to detect samples of different concentrations

Short experimental period

Workflow of CXCL12 Detection at Creative BioMart Biomarker

Creative BioMart Biomarker strictly controls each specific experimental step in the CXCL12 detection procedure to ensure high sensitivity, high accuracy and repeatable CXCL12 detection.

Please feel free to contact us if you would like to know more about CXCL12 detection. At Creative BioMart Biomarker, we not only provide high-quality CXCL12 detection service, but also provide detection services for other biomarkers. Additionally, our experts can also provide and help design the best solution according to your specific requirements.
References:

1. Janssens, R.; et al. The unique structural and functional features of CXCL12. Cell Mol Immunol. 2018, 15(4): 299-311.

2. van der Vorst, E.P.; et al. MIF and CXCL12 in cardiovascular diseases: functional differences and similarities. Front Immunol. 2015, 6: 373.

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CXCL12 is a CXC chemokine that contains multiple alternative splicing forms in humans. CXCL12 is involved in a variety of physiological and pathological processes, including embryogenesis, hematopoiesis, angiogenesis, and inflammatory responses, as well as cardiovascular disease (CVD).

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Biomarkers have huge development potential and research space in many disease research fields. As a leader in companies that provide scientific research outsourcing services for biomarker-related research, Creative BioMart Biomarker not only provides detection services for single biomarkers, multiple biomarkers and imaging biomarkers, but also provides customized biomarker services according to the detail research needs of customers. The high-quality services we provide can meet the service needs of different customers in biomarker research, and are a reliable and long-term partner for our customers.

Why Choose Our Custom Biomarker Service for Related Research?

As a leader in the biomarker industry, Creative BioMart Biomarker has many years of service experience in various fields of biomarker-related research and technology. We have an experts team with rich research experience, an experienced technical team, and several advanced technology platforms. Our expert team is able to customize the appropriate research plan through multiple discussions and negotiations with the client according to your research needs. Our technical team guarantees strict implementation of the experimental plan in accordance with the experimental specifications and regulations to ensure the accuracy and repeatability of the test. In addition, we have been providing customers with comprehensive and detailed experimental data and professional analysis reports. Moreover, our custom biomarker services are highly efficient and cost-effective, and can complete service projects in a short time, helping customers shorten the research cycle and reduce research costs

Our Custom Biomarker Service Can Provide You with Solutions to The Following Problems (including but not limited to):

Workflow of Custom Biomarker Service at Creative BioMart Biomarker

Creative BioMart Biomarker has always had an extraordinary performance in the field of customized biomarker services. Our experts are dedicated to serving customers, ensuring that individual research programs are developed for each project to meet the needs of different customers. We provide 24/7 online services for customers to ensure that any questions and needs of customers on custom biomarker services are resolved as soon as possible. If you are conducting research on biomarkers and are looking for related custom services, Creative BioMart Biomarker will be your ideal choice. Please feel free to contact us, we look forward to being your trusted partner.

Related Services:

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Biomarkers have huge development potential and research space in many disease research fields. As a leader in companies that provide scientific research outsourcing services for biomarker-related research, Creative BioMart Biomarker not only provides detection services for single biomarkers, multiple biomarkers and imaging biomarkers, but also provides customized biomarker services according to the detail research needs of customers.

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C-terminal telopeptide of type I collagen, which is also known as carboxy-terminal collagen I crosslinks (CTX-I), is a biomarker that can be measured as an indication of bone resorption changes and bone diseases. Creative BioMart Biomarker offers high quality detection service for CTX-I, ensuring high detection accuracy, sensitivity and efficiency for each sample.

Introduction

CTX-I is a telopeptide product from the breakdown of type I collagen which contains pyridinium crosslinks. Type I collagen is the most abundant collagen in the human body which is expressed in almost all connective tissues. Type I collagen is an important component of structural proteins in tissues such as bone, skin, tendon, ligament and blood vessels, it accounts for about 95% of the total collagen present in bone and accounts for about 80% of the entire proteins exist in bone. Type I collagen is a triple helix that mainly composed of two α1 chains and one α2 chain. When bone resorption occurs, osteoclasts secrete proteolytic enzymes that degrade type I collagen into fragments, obtain CTX-I and NTX-I finally. Therefore, measuring the expression level of CTX-I in the body can reflect the changes of bone resorption rate, CTX-I can be considered as a biomarker for bone resorption and bone diseases. When osteoclasts degrade type I collagen, CTX-I with different fragment sizes is released into the circulation, most of which are relatively small, they can enter the urine through glomerulus, so urine can be added as a test sample for CTX-I. As bone age increases, the aspartic acid present in CTX-I is converted from its α form to the β form, and β-CTX is then released into the blood and can be used as a specific biomarker for bone resorption. The International Osteoporosis Foundation (IOF) and International Federation of Clinical Chemistry recommend CTX-I as a reference biomarker, in addition, CTX-I can be used as a biomarker for many diseases that are associated with accelerated bone resorption, such as hyperthyroidism, hyperparathyroidism, osteomalacia, rickets, Paget's disease, multiple myeloma, and bone metastases. As a biomarker for bone diseases, the concentration changes of CTX-I may play a role as an assessment indicator for bone diseases diagnostic and antiresorptive therapies.

Figure 1. A schematic diagram to show the main factors that regulate bone remodeling at cellular level and the generation of bone turnover markers during bone remodeling (Glendenning, et al. 2018)

Application of CTX-I Detection

Serum and urine PICP levels as biomarkers to predict different diseases, such as osteomalacia, osteoporosis, bone metastasis, Paget's disease, multiple myeloma, etc.

Our Advantages

Guarantee high accuracy and sensitivity for PICP detection

Ensure high repeatability of PICP detection

Short turn-around time of detection service

Competitive price in the market of detection services

Provide multiple PICP detection methods, including ELISA, CLIA and RIA

Accept a wide range of sample types (serum and urine)

Workflow of CTX-I Detection at Creative BioMart Biomarker

Creative BioMart Biomarker strictly controls each specific experimental step in the CTX-I detection procedure to ensure accurately quantify the level of CTX-I in each sample.

At Creative BioMart Biomarker, we offer CTX-I detection service which includes several technical methods, you can communicate with our experts according to your research needs, and we will determine the final detection technological scheme based on the communication results. Please feel free to contact us, Creative BioMart Biomarker is here to offer you professional and thoughtful service.
References:

1. Glendenning, P.; et al. Clinical utility of bone turnover markers in the management of common metabolic bone diseases in adults. Clinica Chimica Acta. 2018, 481: 161-170.

2. Szulc, P.; et al. Use of CTX-I and PINP as bone turnover markers: national bone health alliance recommendations to standardize sample handling and patient preparation to reduce pre-analytical variability. Osteoporosis International. 2017, 28: 2541-2556.

3. Herrmann, M.; Seibel, M. The amino- and carboxyterminal cross-linked telopeptides of collagen type I, NTX- I and CTX- I: A comparative review. Clinica Chimica Acta. 2008, 393(2): 57-75.

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C-terminal telopeptide of type I collagen, which is also known as carboxy-terminal collagen I crosslinks (CTX-I), is a biomarker that can be measured as an indication of bone resorption changes and bone diseases. Creative BioMart Biomarker offers high quality detection service for CTX-I, ensuring high detection accuracy, sensitivity and efficiency for each sample.

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Chondroitin sulfate 846 epitope (CS846) can be used as a marker for newly synthesized aggrecan molecules in the matrix, and its presence in synovial fluid and serum can reflect the rate of aggrecan synthesis. The reactivity of CS846 was increased in human OA samples, where increased synthesis of aggrecan was generally observed. Creative BioMart Biomarker is capable to provide our customers with superior CS846 detection service, various detection methods ensuring high sensitivity for detecting biomarkers in different samples with different concentrations.

Introduction

Changes in the functional properties and disorders of articular cartilage in osteoarthritis (OA) are the result of changes in matrix macromolecular degradation, synthesis, structure and interaction. During the development of OA, changes occur not only in the degradation of matrix molecules, but also in the rate of synthesis and structure of various matrix components. These changes can represent the response of cells to joint and tissue damage and can serve as metabolic markers of tissue damage. The release of matrix macromolecular fragments and proteases following human OA or joint damage provides evidence of increased matrix turnover. Therefore, increased concentrations of fragmented type II collagen, aggrecan, cartilage oligomeric matrix protein (COMP), and bone sialoprotein (BSP) was detected in articular cartilage or synovial fluid of OA patients, accompanied by significant increase in matrix metalloproteinase 1 (MMP-1) and MMP-3 levels in synovial fluid. Aggrecan is a major component of the extracellular matrix (ECM) in cartilage and is a large proteoglycan. Aggrecan interacts with hyaluronan and link protein and forms large aggregated complexes that fill the interstices of the collagen meshwork. Aggrecan degradation is an important marker of OA, and CS846 is a marker of aggrecan synthesis. The level of CS846 is elevated in cartilage and synovial fluid of OA patients, and related to the progression of joint damage in rheumatoid arthritis (RA).

Figure 1. Structure of proteoglycan aggregates.

Application of CS846

Serum, synovial fluid levels of CS846 can be used as biomarkers for the diagnosis of OA.

Our Advantages

Accept a wide range of sample types

Provide multiple detection methods, including ELISA, fluoro-microbeads guiding chip (FMGC)

Ensure high sensitivity for detecting CS846 in different samples

Ensure high accuracy and repeatable CS846 detection

Ensure a wide kinetic range to detect samples of different concentrations

Short experimental period

Workflow of CS846 Detection at Creative BioMart Biomarker

Creative BioMart Biomarker strictly controls each specific experimental step in the CS846 detection procedure to ensure high sensitivity, high accuracy and repeatable CS846 detection.

Please feel free to contact us if you would like to know more about CS846 detection. At Creative BioMart Biomarker, we not only provide high-quality CS846 detection service, but also provide detection services for other biomarkers. Additionally, our experts can also provide and help design the best solution according to your specific requirements.
References:

1. Lohmander, L.S.; et al. Changes in joint cartilage aggrecan after knee injury and in osteoarthritis. Arthritis Rheum. 1999, 42(3): 534-44.

2. Huang, K.; Wu L.D. Aggrecanase and aggrecan degradation in osteoarthritis. Journal of International Medical Research. 2008, 36: 1149.

3. Jansen, W.D; et al. The combination of the biomarkers urinary C-terminal telopeptide of type II collagen, serum cartilage oligomeric matrix protein, and serum chondroitin sulfate 846 reflects cartilage damage in hemophilic arthropathy. Arthritis Rheum. 2009, 60(1): 290-8.

4. Roughley, P.J.; Mort, J.S. The role of aggrecan in normal and osteoarthritic cartilage. Journal of Experimental Orthopaedics. 2014, 1: 8.

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Chondroitin sulfate 846 epitope (CS846) can be used as a marker for newly synthesized aggrecan molecules in the matrix, and its presence in synovial fluid and serum can reflect the rate of aggrecan synthesis. The reactivity of CS846 was increased in human OA samples, where increased synthesis of aggrecan was generally observed.

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C-peptide is a peptide chain produced when proinsulin breaks down to produce insulin. Its molar amount is equal to that of insulin, so it can be used as a substitute indicator for detecting insulin secretion. C-peptide is related to multiple intracellular signaling pathways in the cell. It is not only a biomarker for the diagnosis of non-diabetic hypoglycemia and insulinoma, but also related to the treatment and prognosis of various complications of diabetes. Creative BioMart Biomarker offers high quality detection service for C-peptide, ensuring high detection accuracy, reproducibility and efficiency.

Introduction

The C-peptide is a peptide chain connecting the A chain and the B chain of insulin in proinsulin, and consists of 31 amino acids. Proinsulin is formed in the endoplasmic reticulum and forms two disulfide bonds between the A and B chains of insulin, and one disulfide bond in the A chain. During the formation of proinsulin, the C-peptide is essential for the correct folding of the two chains of insulin. Proinsulin is then secreted into the Golgi apparatus and packed into vesicles for release. Proinsulin is catalyzed by proinsulin converting enzyme and carboxypeptidase E in the vesicles to undergo proteolysis, producing equimolar amounts of C-peptide and insulin. After insulin and C-peptide are secreted from pancreatic β cells, insulin is largely eliminated in the liver, while the content of C-peptide hardly changes. In addition, because C-peptide has a longer half-life in the blood and has less hemolytic effect, C-peptide can be used as a substitute indicator of insulin secretion in clinical testing. At the same time, the determination of C-peptide can be used for the evaluation of hypoglycemia in non-diabetic patients and the diagnosis of insulinoma. The C-peptide may bind to the G protein-coupled membrane receptor on the membrane structure to increase the intracellular calcium ion concentration and subsequently activate multiple intracellular signaling pathways that depend on calcium ions. The intracellular signaling pathway that C-peptide participates in is related to the pathogenesis and treatment of diabetes. Therefore, C-peptide can be used not only as a biomarker for the diagnosis of non-diabetic hypoglycemia and insulinoma, but also as a treatment and prognostic indicator for various complications of diabetes.

Figure 1. Processing of the proinsulin prohormone (Yosten, 2015)

Application of C-Peptide Detection

Plasma and serum C-peptide levels can be studied as a biomarker in researches related to diabetes mellitus, insulinoma, etc.

Our Advantages

Guarantee high accuracy and sensitivity for C-peptide detection

Ensure high repeatability of C-peptide detection

Short turn-around time of detection service

Competitive price in the market of C-peptide detection services

Multiple technology platforms for C-peptide test services (ELISA, CLIA, etc.)

Accept a wide range of sample types (plasma, serum, tissue homogenates, etc.)

Workflow of C-Peptide Detection at Creative BioMart Biomarker

Creative BioMart Biomarker strictly controls each specific experimental step in the C-peptide detection procedure to ensure accurately quantify the level of C-peptide in each sample.

At Creative BioMart Biomarker, we offer C-peptide detection service which include several technical methods, you can communicate with our experts according to your research needs, and we will determine the final detection technical scheme based on the communication results. Please feel free to contact us, Creative BioMart Biomarker is here to offer you professional and thoughtful service.
References:

1. Hörber, S.; et al. Harmonization of immunoassays for biomarkers in diabetes mellitus. Biotechnology Advances. 2019.

2. Yosten, G.L.C; Kolar, G.R. The physiology of proinsulin C-peptide: unanswered questions and a proposed model. Physiology.2015, 30(4): 327-332.

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C-peptide is a peptide chain produced when proinsulin breaks down to produce insulin. Its molar amount is equal to that of insulin, so it can be used as a substitute indicator for detecting insulin secretion. C-peptide is related to multiple intracellular signaling pathways in the cell.

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Complement component 4 (C4), like complement component 3 (C3), is not only a relatively abundant molecule in complement protein, but also a kind of complement protein which has been studied widely. C4 is abnormally expressed in systemic lupus erythematosus (SLE), glomerulonephritis and other diseases, and may be used as a biomarker for disease diagnosis and monitoring. Creative BioMart Biomarker has many years of experience in biomarker testing and can provide customers with accurate and reliable C4 detection services. High-quality services, short turnaround time and reasonable prices can effectively help customers shorten the research cycle and reduce research costs.

Complement Component 4

C4 participates in the activation pathway of complement system and plays a key role in it. The complement system is a part of the human immune system and a defense system against microbial invaders. The complement system involves dozens of components, including pattern-recognition molecules (PRM), proteases, protein components, regulators and cell surface receptors. When faced with foreign invaders, the combination of PRM and molecular surface patterns can trigger different initiation pathways. The activation pathway of complement system includes the classical pathway (CP), the lectin pathway (LP) and the alternative pathway (AP). The binding of recognition molecule C1q to the antibody complex on the pathogen surface promotes the initiation of CP, which leads to the cleavage of C4 into C4a and C4b, in which C4b surface exposes unique thionolactone ring structure so that C4b can covalently bind to the pathogen surface. Then C2 cleaves to form C2a and C2b.C2a combines with C4b to form C3 convertase C4bC2a. In LP, MASP-1 automatically activates and triggers MASP-2 activation, leading to the cleavage of C4 and C2, which in turn promotes the assembly of C4bC2a and the cleavage of C3, and finally causes further cleavage and activation events. Complement participates in many diseases. Complement deficiency and excessive complement activation are related to the pathogenesis of inflammatory diseases. In the process of inflammation, the expression level of C4 may change, so it can be used as a biomarker in the research of some related diseases to assist in the diagnosis and monitoring of diseases.

Figure 1. The three pathways of complement activation (Beltrame, et al. 2014)

Application of Complement Component 4 Detection

C4 levels can be used as biomarkers in researches related to glomerulonephritis, SLE, etc.

Our Advantages

Guarantee high accuracy and sensitivity for C4 detection

Ensure high repeatability of C4 detection

Short turn-around time of detection service

Competitive price in the market of C4 detection services

Multiple technology platforms for C4 test services (ELISA, CLIA)

Accept a wide range of sample types (plasma, serum, tissue homogenate, etc.)

Workflow of Biomarker Detection at Creative BioMart Biomarker

Creative BioMart Biomarker strictly controls each specific experimental step in the detection procedure to ensure accurately quantify the level of C4 in each sample.

At Creative BioMart Biomarker, we offer high-quality C4 detection service that ensuring the sensitivity and specificity of test results. You can also talk to our experts according to your certain requirement, and we will determine the final detection scheme based on the communication results. Please feel free to contact us, Creative BioMart Biomarker is here to offer you professional and thoughtful service.
References:

1. Andrighetto, S.; et al. Complement and complement targeting therapies in glomerular diseases. International Journal of Molecular Sciences. 2019, 20(24): 6336.

2. Ricklin, D.; Lambris, J. D. Complement in immune and inflammatory disorders: pathophysiological mechanisms. Journal of Immunology. 2013, 190(8): 3831-3838.

3. Beltrame, M. H.; et al. The lectin pathway of complement and rheumatic heart disease. Frontiers in Pediatrics. 2014, 2: 148.

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Complement Component 4 Detection Service

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Complement component 4 (C4), like complement component 3 (C3), is not only a relatively abundant molecule in complement protein, but also a kind of complement protein which has been studied widely. C4 is abnormally expressed in systemic lupus erythematosus (SLE), glomerulonephritis and other diseases, and may be used as a biomarker for disease diagnosis and monitoring.

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Performance of assays kits purchased from manufacturers is highly recommended to be validated before use. Variations caused by lot-lot difference might lead to inconsistent assay results, which could be avoid beforehand by kit validation. Some manufacturers might implement rigorous quality control, while some might not. Necessity of validating the performance of assay kits is therefore apparent. Creative BioMart provides validation service to check the quality of your assay kits with regards to specificity, sensitivity, reproducibility, and linearity.

Assay Kit Validation Workflow

We are able to carry out validation with various types of assay kits, including ELISA kit, enzyme activity assay kit, quantification kit, sample preparation kit, et al. Positive control and negative control samples will be purchased if they are available in market. Otherwise customers are required to provide them for validation. Statistical analysis is presented in the validation report to conclude the performance of your assay kits.

Creative BioMart is experienced on assay kit validation and has gained great reputation in market. We are glad to help you ensure the quality of commercial assay kits, thus allowing smooth experiments. If you are looking for assay kit validation solutions, please contact us for custom service.

Inquiry

We are here to answer any question you may have

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Kit Validation | Creative BioMart - Assay Kit

# data[].attributes.attachment_link.description
Performance of assays kits purchased from manufacturers is highly recommended to be validated before use. Variations caused by lot-lot difference might lead to inconsistent assay results, which could be avoid beforehand by kit validation. Some manufacturers might implement rigorous quality control, while some might not.

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Commercial assay kits are extensively used by scientists and researchers in a broad range of areas, such as biological research, drug development, medical test, food quality control, et al. We are devoted to development of novel assay kits that fill up the blank in market or that serve our valued customers. If the assay kit you are looking for is not available on market yet, please feel free to send a development request to Creative BioMart - Assay Kit. We are ready to help you design and produce your own kits as per your requirement.

Assay Kit Development Workflow

Key Features

We provide custom assay kit service to cover activity determination of different types of enzymes, including but not limited to oxidoreductases, transferases, hydrolases, lyases, isomerases, and ligases. Metabolites and hormones are associated with a number of urgent health issues. Detection of theses biomarkers therefore enables research on metabolic syndrome diagnosis. We comply comprehensive strategies to fulfill requirements of accurate and precise assay of biomarkers of interest. Moreover, assay kits which are used to detect cellular components, biochemical processes, epigenetic modifications, and cell signaling pathways, can be developed and produced by our professional team as well.

Creative BioMart has grown to the global leader in protein and enzyme sicence since last decade. Our collaborators in research institutes, industries, and governments think highly of our top services and products. Our mission is to make your assay quick, easy to handle, cost-effective, accurate, and reproducible. Please contact us if you plan to creative your own assay kit.

Inquiry

We are here to answer any question you may have

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Kit Development | Creative BioMart - Assay Kit

# data[].attributes.attachment_link.description
Commercial assay kits are extensively used by scientists and researchers in a broad range of areas, such as biological research, drug development, medical test, food quality control, et al. We are devoted to development of novel assay kits that fill up the blank in market or that serve our valued customers.

# data[].attributes.attachment_link.readable_text
Ingredients and additives in food and cosmetics constantly draw public attention due to their potential impact on human health. Quality control plays a critical role before food product and cosmetics become available in the market. Creative BioMart endeavors to provide food and cosmetics related substances testing service using our assay kits to ensure test reliability, sensitivity, and specificity.

Our tests by assay kit cover a wide range of substances present or potentially present in food and cosmetics. Generally, your food product sample is processed and subject to quantification of acidity, hydrogen peroxide, peroxide value, carbonyl value, iodine value, soap content, unsaponifiable matter, pesticide residue, sibutramine et al. We also provide quick detection of contaminant microorganisms found in food product, such as Aspergillus, Penicillium, E. coli, et al. It is also available to test cosmetics sample concerning D-sorbitol, nitroimidazole, minoxidil, hydroquinone, sulfonamides, formaldehyde, boric acid, lincomycin, et al. As heavy metal accumulation prevalent in plants and animals, it comes imperative to assure the safe level of them in food and cosmetics. We are experienced in quick test of calcium, copper, iron, magnesium, cadmium, mercury, aluminum, nickel, cobalt, zinc, lead, tin, manganese, chromium, selenium, et al.

Creative BioMart is proud to serve you with sensitive and reliable food and cosmetics related substances testing in a short turnaround time. Please feel free to contact us if you plan to have your sample tested.

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Food and Cosmetics Related Substances Test | Creative BioMart - Assay Kit

# data[].attributes.attachment_link.description
Ingredients and additives in food and cosmetics constantly draw public attention due to their potential impact on human health. Quality control plays a critical role before food product and cosmetics become available in the market. Creative BioMart endeavors to provide food and cosmetics related substances testing service using our assay kits to ensure test reliability, sensitivity, and specificity.

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Cellular and Biochemical Assays | Creative BioMart - Assay Kit

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Metabolism and Endocrinology Assays | Creative BioMart - Assay Kit

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Performance of assays kits purchased from manufacturers is highly recommended to be validated before use. Variations caused by lot-lot difference might lead to inconsistent assay results, which could be avoid beforehand by kit validation. Some manufacturers might implement rigorous quality control, while some might not. Necessity of validating the performance of assay kits is therefore apparent. Creative BioMart provides validation service to check the quality of your assay kits with regards to specificity, sensitivity, reproducibility, and linearity.

Assay Kit Validation Workflow

We are able to carry out validation with various types of assay kits, including ELISA kit, enzyme activity assay kit, quantification kit, sample preparation kit, et al. Positive control and negative control samples will be purchased if they are available in market. Otherwise customers are required to provide them for validation. Statistical analysis is presented in the validation report to conclude the performance of your assay kits.

Creative BioMart is experienced on assay kit validation and has gained great reputation in market. We are glad to help you ensure the quality of commercial assay kits, thus allowing smooth experiments. If you are looking for assay kit validation solutions, please contact us for custom service.

Inquiry

We are here to answer any question you may have

# data[].attributes.attachment_link.title
Kit Validation | Creative BioMart - Assay Kit

# data[].attributes.attachment_link.description
Performance of assays kits purchased from manufacturers is highly recommended to be validated before use. Variations caused by lot-lot difference might lead to inconsistent assay results, which could be avoid beforehand by kit validation. Some manufacturers might implement rigorous quality control, while some might not.

# data[].attributes.attachment_link.readable_text
Ingredients and additives in food and cosmetics constantly draw public attention due to their potential impact on human health. Quality control plays a critical role before food product and cosmetics become available in the market. Creative BioMart endeavors to provide food and cosmetics related substances testing service using our assay kits to ensure test reliability, sensitivity, and specificity.

Our tests by assay kit cover a wide range of substances present or potentially present in food and cosmetics. Generally, your food product sample is processed and subject to quantification of acidity, hydrogen peroxide, peroxide value, carbonyl value, iodine value, soap content, unsaponifiable matter, pesticide residue, sibutramine et al. We also provide quick detection of contaminant microorganisms found in food product, such as Aspergillus, Penicillium, E. coli, et al. It is also available to test cosmetics sample concerning D-sorbitol, nitroimidazole, minoxidil, hydroquinone, sulfonamides, formaldehyde, boric acid, lincomycin, et al. As heavy metal accumulation prevalent in plants and animals, it comes imperative to assure the safe level of them in food and cosmetics. We are experienced in quick test of calcium, copper, iron, magnesium, cadmium, mercury, aluminum, nickel, cobalt, zinc, lead, tin, manganese, chromium, selenium, et al.

Creative BioMart is proud to serve you with sensitive and reliable food and cosmetics related substances testing in a short turnaround time. Please feel free to contact us if you plan to have your sample tested.

# data[].attributes.attachment_link.title
Food and Cosmetics Related Substances Test | Creative BioMart - Assay Kit

# data[].attributes.attachment_link.description
Ingredients and additives in food and cosmetics constantly draw public attention due to their potential impact on human health. Quality control plays a critical role before food product and cosmetics become available in the market. Creative BioMart endeavors to provide food and cosmetics related substances testing service using our assay kits to ensure test reliability, sensitivity, and specificity.

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Cellular and Biochemical Assays | Creative BioMart - Assay Kit

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Platelet Activating Factor Acetylhydrolase (PAF-AH)

Specification

Drug Information

Related Products

Product Name:

Platelet Activating Factor Acetylhydrolase (PAF-AH)

Description:

Recombinant Human PAF-AH is a 420 amino acid glycoprotein which migrates with an apparent molecular mass of 47-55 kDa by SDS-PAGE analysis. Recombinan Human PAF-AH has a calculated, theoretical molecular weight of 47.8 kDa.

Introduction:

Platelet Activating Factor (PAF) is a biologically active phospholipid, which exerts primarily proinflammatory activities by specifically signaling through G-protein-coupled receptors on platelets, neutrophils, and monocytes. Platelet Activating Factor Acetylhydrolase (PAF-AH) is a secreted protein that mediates PAF activity by specifically catalyzing hydrolysis of the "sn2" ester bond, resulting in the conversion of PAF to the biologically inactive lyso-PAF. PAF-AH can also interact with LDL particles to induce the hydrolysis of LDL-associated, oxidized phospholipids, generating lysophosphatidylcholine (lyso-PC) and other lysophospholipids.

Sequences:

FDWQYINPVA HMKSSAWVNK IQVLMAAASF GQTKIPRGNG PYSVGCTDLM FDHTNKGTFL RLYYPSQDND RLDTLWIPNK EYFWGLSKFL GTHWLMGNIL RLLFGSMTTP ANWNSPLRPG EKYPLVVFSH GLGAFRTLYS AIGIDLASHG FIVAAVEHRD RSASATYYFK DQSAAEIGDK SWLYLRTLKQ EEETHIRNEQ VRQRAKECSQ ALSLILDIDH GKPVKNALDL KFDMEQLKDS IDREKIAVIG HSFGGATVIQ TLSEDQRFRC GIALDAWMFP LGDEVYSRIP QPLFFINSEY FQYPANIIKM KKCYSPDKER KMITIRGSVH QNFADFTFAT GKIIGHMLKL KGDIDSNVAI DLSNKASLAF LQKHLGLHKD FDQWDCLIEG DDENLIPGTN INTTNQHIML QNSSGIEKYN

Molecular Weight:

47.8 kDa

Synonyms:

PAF acetylhydrolase; PAF 2-acylhydrolase; LDL-associated phospholipase A2; LDL-PLA(2); 2-acetyl-1-alkylglycerophosphocholine esterase; 1-alkyl-2-acetylglycerophosphocholine esterase;

Biological Activity:

Measured by its ability to cleave a PAF analog in a chromogenic substrate linked assay. At a PAF-AH concentration of 10.0 μg/ml, 50% cleavage was achieved at an incubation time of approximately 2 minutes.

Purity:

≥95% by SDS-PAGE gel and HPLC analyses.

Applications:

Platelet Activating Factor Acetylhydrolase (PAF-AH) is a secreted protein that mediates PAF activity by specifically catalyzing hydrolysis of the "sn2" ester bond, resulting in the conversion of PAF to the biologically inactive lyso-PAF.

Examples of Clinical Use:

Catalyzing hydrolysis of the "sn2" ester bond, resulting in the conversion of PAF to the biologically inactive lyso-PAF.

Affected organisms:

Humans

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Platelet Activating Factor Acetylhydrolase (PAF-AH) - Therapeutic Proteins

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Specification Drug Information Related Products Product Name: Platelet Activating Factor Acetylhydrolase (PAF-AH) Description: Recombinant Human PAF-AH is a 420 amino acid glycoprotein which migrates with an apparent molecular mass of 47-55 kDa by SDS-PAGE analysis. Recombinan Human PAF-AH has a calculated, theoretical molecular weight of 47.8 kDa.

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Specification

Drug Information

Related Products

Product Name:

Recombinant Angiostatin

Description:

Recombinant Angiostatin (98-259 aa) is a single, non-glycosylated polypeptide chain, it represents a proteolytic fragment of plasminogen containing the first three kringle structures.

Introduction:

Angiostatin is an internal proteolytic fragment of plasminogen, containing at least three Kringle domains. The Kringle domains have specific anti-migratory and anti-proliferative activities. Angiostatin, an endogenous angiogenesis inhibitor, inhibits endothelial cell proliferation in vitro and angiogenesis in vivo. It is a potent and specific natural inhibitor of angiogenisis and metastatic tumor growth.

Sequences:

VYLSECKTGN GKNYRGTMSK TKNGITCQKW SSTSPHRPRF SPATHPSEGL EENYCRNPDN DPQGPWCYTT DPEKRYDYCD ILECEEECMH CSGENYDGKI SKTMSGLECQ AWDSQSPHAH GYIPSKFPNK NLKKNYCRNP DRELRPWCFT TDPNKRWELC DIPRCTTPPP SSGPTYQCLKGTGENYRGNV AVTVSGHTCQ HWSAQTPHTH NRTPENFPCK NLDENYCRNP DGKRAPWCHT TNSQVRWEYC KIPSCDSSP

Source:

Escherichia coli

Molecular Weight:

30 kDa

Synonyms:

Recombinant Angiostatin;

Biological Activity:

The activity is assayed on anti-proliferation and anti-migration of endothelial cells in vitro and anti-angiogenesis in vivo. The specific activity of anti-migration of endothelial cells in vitro is 0.55×105 Units/mg.

Formulation:

Lyophilised

Storage:

Shipped at 4 centigrade. Store at -20 centigrade. Store under desiccating conditions. pH: 5.50. Constituents: 4% Mannitol, 0.16% Sodium acetate.

Reconstitution:

Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, subdivide the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles.

Applications:

Angiostatin, an endogenous angiogenesis inhibitor, inhibits endothelial cell proliferation in vitro and angiogenesis in vivo. It is a potent and specific natural inhibitor of angiogenisis and metastatic tumor growth.

Examples of Clinical Use:

Natural inhibitor of angiogenisis and metastatic tumor growth.

Affected organisms:

Humans

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Recombinant Angiostatin - Therapeutic Proteins

# data[].attributes.attachment_link.description
Specification Drug Information Related Products Product Name: Recombinant Angiostatin Description: Recombinant Angiostatin (98-259 aa) is a single, non-glycosylated polypeptide chain, it represents a proteolytic fragment of plasminogen containing the first three kringle structures. Introduction: Angiostatin is an internal proteolytic fragment of plasminogen, containing at least three Kringle domains.

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Specification

Drug Information

Related Products

Product Name:

Native Angiostatin

Description:

Angiostatin is an internal proteolytic fragment of plasminogen, containing at least three Kringle domains.

Introduction:

The Kringle domains have specific anti-migratory and anti-proliferative activities. Angiostatin, an endogenous angiogenesis inhibitor, inhibits endothelial cell proliferation in vitro and angiogenesis in vivo. It is a potent and specific natural inhibitor of angiogenisis and metastatic tumor growth.

Synonyms:

Native Angiostatin;

Storage:

Shipped on dry ice. Upon delivery aliquot and store at -80 centigrade. Avoid freeze / thaw cycles. pH: 7.40. Constituents: 0.476% HEPES, 0.87% Sodium chloride.

Reconstitution:

Constituents: 0.476% HEPES, 0.87% Sodium chloride.

Applications:

Angiostatin, an endogenous angiogenesis inhibitor, inhibits endothelial cell proliferation in vitro and angiogenesis in vivo. It is a potent and specific natural inhibitor of angiogenisis and metastatic tumor growth.

Examples of Clinical Use:

Natural inhibitor of angiogenisis and metastatic tumor growth.

Affected organisms:

Humans

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Native Angiostatin - Therapeutic Proteins

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Specification Drug Information Related Products Product Name: Native Angiostatin Description: Angiostatin is an internal proteolytic fragment of plasminogen, containing at least three Kringle domains. Introduction: The Kringle domains have specific anti-migratory and anti-proliferative activities. Angiostatin, an endogenous angiogenesis inhibitor, inhibits endothelial cell proliferation in vitro and angiogenesis in vivo.

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Beta-Glucocerebrosidase (GBA)

Specification

Drug Information

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Product Name:

Beta-Glucocerebrosidase (GBA)

Description:

GBA produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 503 amino acids (40-536a.a.) and having a molecular mass of 56.4kDa. (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). GBA is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Introduction:

Beta-Glucocerebrosidase, also known as GBA is amember of the glycosyl hydrolase 30 family. GBA is a lysosomal enzyme which requires a signal peptide for transport across the membrane of the rough endoplasmic reticulum as well as glycosylation for transport into lysosomes. Furthermore, Gaucher disease is caused by a deficiency in the activity of the enzyme glucocerebrosidase.

Sequences:

ARPCIPKSFG YSSVVCVCNA TYCDSFDPPT FPALGTFSRY ESTRSGRRME LSMGPIQANH TGTGLLLTLQ PEQKFQKVKG FGGAMTDAAA LNILALSPPA QNLLLKSYFS EEGIGYNIIR VPMASCDFSI RTYTYADTPD DFQLHNFSLP EEDTKLKIPL IHRALQLAQR PVSLLASPWT SPTWLKTNGA VNGKGSLKGQ PGDIYHQTWA RYFVKFLDAY AEHKLQFWAV TAENEPSAGL LSGYPFQCLG FTPEHQRDFI ARDLGPTLAN STHHNVRLLM LDDQRLLLPH WAKVVLTDPE AAKYVHGIAV HWYLDFLAPA KATLGETHRL FPNTMLFASE ACVGSKFWEQ SVRLGSWDRG MQYSHSIITN LLYHVVGWTD WNLALNPEGG PNWVRNFVDS PIIVDITKDT FYKQPMFYHL GHFSKFIPEG SQRVGLVASQ KNDLDAVALM HPDGSAVVVV LNRSSKDVPL TIKDPAVGFL ETISPGYSIH TYLWRRQ HHH HHH

Source:

Sf9 Baculovirus Cells

Molecular Weight:

56.4 kDa

Synonyms:

GBA Human; Beta-Glucocerebrosidase Human Recombinant; Glucosidase; Beta; Acid; D-Glucosyl-N-Acylsphingosine Glucohydrolase; Beta-Glucocerebrosidase; Acid Beta-Glucosidase; Glucosylceramidase; Imiglucerase; Alglucerase; EC 3.2.1.45; Beta-GC; GLUC; Glucosidase; Beta; Acid (Includes Glucosylceramidase); Glucosylceramidase-Like Protein; Lysosomal Glucocerebrosidase; GBA1; GCB; GC; Glucosylceramidase;

Purity:

>80% as determined by SDS-PAGE.

Formulation:

Sterile Filtered colorless solution. GBA protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH7.4) and 10% glycerol.

Storage:

Store at 4 centigrade if entire vial will be used within 2-4 weeks. Store, frozen at -20 centigrade for longer periods of time. Avoid multiple freeze-thaw cycles.

Applications:

Treating Gaucher disease.

Examples of Clinical Use:

Treating Gaucher disease.

Affected organisms:

Humans

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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GBA produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 503 amino acids (40-536a.a.) and having a molecular mass of 56.4kDa. (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). GBA is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

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Bacterial/Permeability-Increasing Protein (BPI)

Specification

Drug Information

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Product Name:

Bacterial/Permeability-Increasing Protein (BPI)

Description:

The native protein BPI is isolated from human buffy coats and has a molecular mass of 53kDa. BPI encodes a lipopolysaccharide binding protein. BPI is related to human neutrophil granules and has bactericidal activity on gram-negative organisms.

Introduction:

Bacterial/Permeability-Increasing Protein (BPI) is present in the azurophilic granules of polymorphonuclear leukocytes (PMN). BPI is toxic only toward Gram-negative bacteria. This specificity is attributable to the strong attraction of BPI for the lipopolysaccharides (LPS) in the bacterial envelope. BPI is also an important antigen for anti-neutrophil cytoplasmic autoantibodies (ANCA) in vasculitis.

Molecular Weight:

53 kDa

Synonyms:

BPI Human; Bactericidal/Permeability-Increasing Protein Human; Bactericidal/permeability-increasing protein; CAP 57;

Purity:

>70% as determined by SDS-PAGE and capillary electrophoresis.

Formulation:

Sterile filtered colorless solution. BPI (1.15mg/ml) is supplied in 25mM Sodium Acetate pH-4.6.

Storage:

Store at 4 centigrade if entire vial will be used within 2-4 weeks. Store, frozen at -20 centigrade for longer periods of time. Avoid multiple freeze-thaw cycles.

Applications:

BPI is toxic only toward Gram-negative bacteria. BPI is also an important antigen for anti-neutrophil cytoplasmic autoantibodies (ANCA) in vasculitis.

Examples of Clinical Use:

Has bactericidal activity on gram-negative organisms.

Affected organisms:

Humans

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Specification Drug Information Related Products Product Name: Bacterial/Permeability-Increasing Protein (BPI) Description: The native protein BPI is isolated from human buffy coats and has a molecular mass of 53kDa. BPI encodes a lipopolysaccharide binding protein. BPI is related to human neutrophil granules and has bactericidal activity on gram-negative organisms. Introduction: Bacterial/Permeability-Increasing Protein (BPI) is present in the azurophilic granules of polymorphonuclear leukocytes (PMN).

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Human Transforming Growth Factor-Beta 3 (TGFB3)

Specification

Drug Information

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Product Name:

Human Transforming Growth Factor-Beta 3 (TGFB3)

Description:

TGFbeta 3 (transforming growth factor-beta 3) is a member of a TGF-beta superfamily subgroup that is defined by their structural and functional similarities.

Introduction:

TGF-beta 3 and its closely related proteins, TGF-beta 1 and -beta 2, act as cellular switches to regulate immune function, cell proliferation, and epithelial-mesenchymal transition. The non-redundant biological effects of TGF- beta 3 include involvement in palatogenesis, chondrogenesis, and pulmonary development. Human TGF- beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 20 aa signal peptide and a 392 aa proprotein. The proprotein is processed by a furinlike convertase to generate a 220 aa latencyassociated peptide (LAP) and a 112 aa mature TGF-beta 3. Mature human TGF-beta 3 shows 100%, 99%, and 98% aa identity with mouse/dog/horse, rat, and pig TGF- beta 3, respectively. TGF-beta 3 is secreted as a complex with LAP. This latent form of TGF-beta 3 becomes active upon cleavage by plasmin, matrix metalloproteases, thrombospondin-1, and a subset of integrins. TGF-beta 3 binds with high affinity to TGF-beta RII, a type II serine/threonine kinase receptor. This receptor then phosphorylates and activates type I serine/threonine kinase receptors, TGF- beta RI or ALK-1, to modulate transcription through Smad phosphorylation. The divergent biological effects exerted by individual TGF-beta isoforms is dependent upon the recruitment of co-receptors (TGF- beta RIII and endoglin) and the subsequent initiation of Smad-dependent or -independent signaling pathways.

Source:

Baculovirus infected Sf21 cells

Molecular Weight:

Predicted Molecular Mass: 12.7 kDa (monomer). SDS-PAGE: 12 kDa, reducing conditions; 24 kDa, non-reducing conditions.

Synonyms:

ARVD; FLJ16571; TGFB3; TGFbeta 3; TGF-beta 3; TGF-beta3; TGF-beta-3; transforming growth factor beta-3; transforming growth factor, beta 3;

Biological Activity:

Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.01-0.04 ng/mL. The specific activity of recombinant human TGF-beta 3 is approximately 2.2 x 104 IU/μg, which is calibrated against recombinant human TGF-beta 3 WHO International Standard (NIBSC code: 09/234).

Purity:

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Formulation:

Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.

Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution:

Reconstitute at 20 μg/mL in sterile 4 mM HCl containing 1 mg/mL human or bovine serum albumin.

Endotoxin Level:

<0.01 EU per 1 μg of the protein by the LAL method.

Applications:

The non-redundant biological effects of TGF- beta 3 include involvement in palatogenesis, chondrogenesis, and pulmonary development.

Examples of Clinical Use:

Promoting palatogenesis, chondrogenesis, and pulmonary development

Affected organisms:

Humans

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Human Transforming Growth Factor-Beta 3 (TGFB3) - Therapeutic Proteins

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Specification Drug Information Related Products Product Name: Human Transforming Growth Factor-Beta 3 (TGFB3) Description: TGF­beta 3 (transforming growth factor-beta 3) is a member of a TGF­-beta superfamily subgroup that is defined by their structural and functional similarities. Introduction: TGF-beta 3 and its closely related proteins, TGF-beta 1 and ­-beta 2, act as cellular switches to regulate immune function, cell proliferation, and epithelial­-mesenchymal transition.

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Recombinant Human Thrombopoietin (TPO)

Specification

Drug Information

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Product Name:

Recombinant Human Thrombopoietin (TPO)

Description:

Thrombopoietin (Tpo) (Ser22-Gly353), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c‑mpl.

Introduction:

Defects in the Tpo‑Tpo R signaling pathway are associated with a variety of platelet disorders. The 353 amino acid (aa) human Tpo precursor is cleaved to yield the 332 aa mature protein. Mature human Tpo shares approximately 70% aa sequence homology with mouse and rat Tpo. It is an 80‑85 kDa protein that consists of an N‑terminal domain with homology to Erythropoietin (Epo) and a C‑terminal domain that contains multiple N‑linked and O‑linked glycosylation sites. Tissue specific alternate splicing of human Tpo generates multiple isoforms with internal deletions, insertions, and/or C‑terminal substitutions. Tpo promotes the differentiation, proliferation, and maturation of MK and their progenitors. Several other cytokines can promote these functions as well but only in cooperation with Tpo. Notably, IL‑3 independently induces MK development, although its effects are restricted to early in the MK lineage. Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation. It is cleaved by platelet‑derived thrombin following Arg191 within the C‑terminal domain and subsequently at other sites upon extended digestion. Full length Tpo and shorter forms circulate in the plasma. The C‑terminal domain is not required for binding to Tpo R or inducing MK growth and differentiation. Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia‑sensitized neurons and inhibits neuronal differentiation by blocking NGF induced signaling.

Source:

Baculovirus infected Sf21 cells

Molecular Weight:

Predicted Molecular Mass: 35 kDa SDS-PAGE: 43-60 kDa, reducing conditions

Synonyms:

Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; megakaryocyte stimulating factor; MGDF; MGDFC-mpl ligand; MK-CSF; ML; MPL ligand; MPLLGMGC163194; Myeloproliferative leukemia virus oncogene ligand; THPO; thrombopoietin nirs variant 1; Thrombopoietin; Tpo; TPOMKCSF;

Biological Activity:

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED50 for this effect is 0.3-3 ng/mL.

Purity:

>97%, by SDS-PAGE with silver staining

Formulation:

Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.

Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -70 centigrade under sterile conditions after reconstitution.

Reconstitution:

Reconstitute at 50-200 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Endotoxin Level:

<1.0 EU per 1 μg of the protein by the LAL method.

Applications:

Tpo promotes platelet production, aggregation, ECM adhesion, and activation.

Examples of Clinical Use:

Promoting platelet production, aggregation, ECM adhesion, and activation.

Affected organisms:

Humans

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Recombinant Human Thrombopoietin (TPO) - Therapeutic Proteins

# data[].attributes.attachment_link.description
Specification Drug Information Related Products Product Name: Recombinant Human Thrombopoietin (TPO) Description: Thrombopoietin (Tpo) (Ser22-Gly353), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c‑mpl. Introduction: Defects in the Tpo‑Tpo R signaling pathway are associated with a variety of platelet disorders.

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Transforming Growth Factor-Alpha (TGFA)

Specification

Drug Information

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Product Name:

Transforming Growth Factor-Alpha (TGFA)

Description:

TGFA Human Recombinant (40-89) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 50 amino acids and having a molecular mass of 6kDa. The TGFA is purified by proprietary chromatographic techniques.

Introduction:

Transforming Growth Factor-Alpha (TGF-alpha) belongs to the EGF family of cytokines. TGFA soluble form is discharged from the membrane by proteolytic cleavage. Membrane-bound proTGF-alpha is biologically active and has a role in cell-cell adhesion or in the stimulation of adjacent cells. TGFA expression is common in transformed cells. Additionally, TGFA is expressed in normal tissues during embryogenesis and in adult cells/tissues, including the pituitary, keratinocytes, and macrophages.

Sequences:

VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA.

Molecular Weight:

6 kDa

Synonyms:

Transforming Growth Factor Alpha; Protransforming Growth Factor Alpha; TGF-Alpha; TGFA;

Biological Activity:

The ED50, as measured in a proliferation assay using mouse Balb/3T3 cells, is less than 0.4ng/ml.

Purity:

>97.0% as determined by SDS-PAGE.

Formulation:

Sterile Filtered White lyophilized (freeze-dried) powder. Lyophilized from a 0.2µm filtered solution in Acetonitrile and TFA.

Storage:

Lyophilized TGFA although stable at room temperature for 3 weeks, should be stored desiccated below -18 centigrade. Upon reconstitution TGFA should be stored at 4 centigrade between 2-7 days and for future use below -18 centigrade. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Reconstitution:

It is recommended to reconstitute the lyophilized TGFA in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Applications:

Membrane-bound proTGF-alpha is biologically active and has a role in cell-cell adhesion or in the stimulation of adjacent cells.

Examples of Clinical Use:

Has a role in cell-cell adhesion or in the stimulation of adjacent cells.

Affected organisms:

Humans

For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Transforming Growth Factor-Alpha (TGFA) - Therapeutic Proteins

# data[].attributes.attachment_link.description
Specification Drug Information Related Products Product Name: Transforming Growth Factor-Alpha (TGFA) Description: TGFA Human Recombinant (40-89) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 50 amino acids and having a molecular mass of 6kDa. The TGFA is purified by proprietary chromatographic techniques. Introduction: Transforming Growth Factor-Alpha (TGF-alpha) belongs to the EGF family of cytokines.

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Creative BioMart provides you with endotoxin-related Supporting reagents, in order to complete the detection and removal of endotoxins more accurately and without interference.

Mainly provide Tris HCl Buffer, MgCl ₂, Endotoxin-free Blood Anticoagulant，Glucan Blocker and Dispersing Agent.

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Creative BioMart provides you with endotoxin-related Supporting reagents, in order to complete the detection and removal of endotoxins more accurately and without interference. Mainly provide Tris HCl Buffer, MgCl 2, Endotoxin-free Blood Anticoagulant，Glucan Blocker and Dispersing Agent.

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Endotoxin (LPS) preparations are widely used to study the structure, metabolism, immunology, and biosynthesis of LPS. LPS is also used to induce the synthesis and secretion of growth-promoting factors such as interleukins. By combining LPS with FITC, TRITC, and TNP, it can be used to study intracellular responses such as immune response to LPS.

Creative BioMart offers a variety of LPS products, including a variety of bacterial sources, and various labeled LPS.

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Endotoxin (LPS) preparations are widely used to study the structure, metabolism, immunology, and biosynthesis of LPS. LPS is also used to induce the synthesis and secretion of growth-promoting factors such as interleukins. By combining LPS with FITC, TRITC, and TNP, it can be used to study intracellular responses such as immune response to LPS.

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