# data[].attributes.body
The technology of preparing recombinant DNA in vitro by cutting up DNA molecules and splicing together fragments from more than one organism

# data[].attributes.headline
1. b) Name the source of plasmid and what is the role in the cloning process?

# data[].attributes.subject
1. b) Name the source of plasmid and what is the role in the cloning process?

# data[].attributes.body
Source : Bacteria cell
Role  : Can used in genetic engineering to amplify, or produce many copies of, certain genes.

# data[].attributes.body
An endonuclease that recognize and cleave DNA molecule at specific nucleotide sequences

# data[].attributes.body
Restriction site: GAATTC
Restriction enzyme: BamHl

# data[].attributes.body
i. 4
ii. 4

# data[].attributes.body
DNA Ligase

# data[].attributes.body
Bacteria is suitable as a host cell due to the capable of receiving recombinant DNA through the transformation process. It is also able to maintain the structure of the recombinant DNA and amplify the gene product from recombinant DNA.

# data[].attributes.body
Q is most suitable to be a cloning vector.

# data[].attributes.body
A suitable cloning vector is able to accept foreign DNA in multiple cloning sites (MCS).
Besides, they contain an origin of replication which they are able to replicate freely in the host cell. Moreover, they contain genetic markers for screening purpose such as ampR.

# data[].attributes.body
Recombinant DNA

# data[].attributes.body
Host cell

# data[].attributes.body
2. e) The two characteristic of T are able to receive the recombinant DNA through the transformation process and able to express the gene product of the recombinant DNA .

# data[].attributes.body
2. f) No , it’s not possible to use different types of s for vector and target gene . It is because in order to produce compatible sticky ends , we need to cleaved with the same restriction enzymes.

# data[].attributes.body
EcoRI cleave and produce sticky end while SmaI cleave and produce blunt end. Besides, EcoRI cleave the restriction site between nucleotide G and A whereas SmaI cleave the restriction side between nucelotide G and C.

# data[].attributes.body
i) staggered cut with single-stranded end.

ii) 5’ AATT 3’

iii) 2 pieces

# data[].attributes.body
White and blue colonies are formed. White colonies indicated that the recombinant plasmid while the blue colonies indicated the non-recombinant plasmid.

# data[].attributes.body
4. b) Bacteria that culture in medium that contains ampicillin will survive.