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Transformation efficiency = Number of Colonies/Amount of DNA spread on agar plate = 1/.16 = 6.25 transformants/ug

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DNA spread = (total amount of DNA) x (fraction of DNA) = .8 x .196 = .16 ug

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Fraction of DNA used = Volume Spread on LB/amp plate/Total Volume in Test Tube =100/510 = .196

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Amount of pGLO DNA (ug) = (concentraton of DNA) x (volume of DNA) = 10 x .008 = .8

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Since we were able to successfully produce a colony that had expressed the GFP gene from the bioluminescent jellyfish, it proves that it is possible for bacteria to produce traits from other species. Therefore, our guiding claim was correct.

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The evidence we collected was based off of the observations we saw from the colonies grown on the LB/amp/agar plate, which totaled to one colony. From there we could determine the amount of pGLO DNA spread, which can be calculated by multiplying the total amount of DNA used by the fraction of DNA used, in this experiment it came out to around 0.2. Using these two numbers, the transformation efficiency can be calculated, as it is the total number of colonies growing on the agar plate divided by the amount of DNA spread on the agar plate, which totaled to 6.25 (transformants/ug).

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Yes, the insertion of the GFP gene from bioluminescent jellyfish into e. coli can change/transform e. coli into a bioluminescent form/phenotype by expression of the GFP gene into the green fluorescent protein that glows green.

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Can the insertion of the GFP gene from bioluminescent jellyfish into e. coli change/transform e. coli into a bioluminescent form/phenotype by expression of the GFP gene into the green fluorescent protein that glows green?

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By: Erica Olsen, Enun Enun, and Sami Guiterrez

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By: Erica Olsen, Enun Enun, and Sami Guiterrez