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Iago and Othello talk about Desdemonna and Cassio when Othello says this:"Certain,men should be what they seem.".
3.3.133
This quote shows us how Iago uses Othello's misguided trusted belief to slowly plant insecurities in his mind so he can suspect his wife.

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Iago increases Othello's jealousies by hesitating to tell him what he is thinking.
O, beware, my lord, of jealousy! It is the green-eyed monster which doth mock The meat it feeds on.
Jealous and envy has better effect when its delivered stealthily and subtly as it further taunts a person's insecurities.
3.3.169-171

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At the very beginning of the play, we see Iago's source of beef with Othello: envy for a position."But he ,sir had th' election.And I of whom his eyes had seen proof At Rhodes, ,at Cyprus, and on other grounds."
1.1.28-29 p.4
Explanation: Here we see that jealousy is a corrosive feeling that doesn't stay confined to a character but also to all that characters' supporters, thus being a greater menace.

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Brabantio arrives to arrest Othello for dnapping his daughter. "Of such a thing as thou-to fear, not delight./Judge me the world if 'tis not gross in sense/ That thou hast practiced on her with foul charms/Abused her delicate youth with drugs or minerals."
1.2
Brabantio thinks his daughter has been bewitched because of Othello'sidentity as a Moor.

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Iago explains Desdemonna's possible behaviour after Cassio uncomfortably leaves her.She that, so young, could give out such a seeming, /To seel her father's eyes up close an oak,/He thought 'twas witchcraft.
3.3.213-215

Explanation:Iago is putting the idea that his wife is manipulating him, which is a good tactic to distract Othello's attention from the real liar.

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Right after a drinking brawl, Cassio asks Iago for help after he nearly gets fired from lieutenant position.Iago's response is to go see Desdemonna for advice so Cassio can be suspected to have seduced Othello's wife."When devils will the blackest sins pull off will the blackest sins put on /They do suggest at first with heavenly shows."
2.3.331-334 p.114
Iago reveals that the best to commit sins is to remain disguised.

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After speaking of his schemes to Roderigo to destroy Cassio, Iago closes the scene with a soliloquy."For that I do suspect the lusty/ Hath leaped into my seat.The thought wherof /Doth, like a poisonous mineral, gnaw my inwards,/And nothing can or shall content my soul./Till I an evened with him wife for wife."
2.1.289-293 p.84
Explanation:  Although there is no evidence yet, the mere image of Othello sleeping with his wife is enough for Iago to twist his already bitter nature to cause Othello to mlose his reputation

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1. Context  ( Who? Where? When?)
2.   The quote... written in Shakespeare’s words (Early Modern English)
5.   Reference:  (1.2.45)
6.   Explanation of the link between the quote and the theme.

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After waking Brabantio up, Roderigo informs the senator of his daughter's betrayal and secret marriage with a racial outcast, much to Brabantio's rage."Call up  my brother.-Oh would you had had her./Some one way,some another. Do you know/Where we may apprehend her and the Moor?
1.1.178-180  p.14
Explanation: In spite of previously respecting Othello due to his military skills, Brabantio has an immediate change of heart abou t him when he realises his daughter is married to a Moor.Because of the Venitians prejudice towards people of color, Desemonna's father is worried for his reputation of being connected to social outsiders

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From the dark streets of Venice, Iago and Rodrigo wake up the senator with shocking news.     In an attempt to damage the reputation of Othello and infuriate the senator, Iago shouts up to Brabantio,  "... you have lost half of your soul.  Even now, very now, an old black ram is tupping your white ewe. ... you'll have your daughter covered with a Barbary horse.  You'll have your nephews neigh to you"
( 1.1.89-91 and 114-116)

Explanation: This shows Iago’s racist attitude towards Othello.   He uses animal imagery to make true love and an honest, loving sexual encounter between Othello and Desdemona seem bestial, violent, and nasty.

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On the dark streets of Venice, Iago is telling his puppet Roderigo about his 2-faced nature. Iago explains,  "I follow him to serve my turn upon him. ... Others there are who, trimmed in forms and visages of duty, keep their hearts attending on themselves and, throwing but shows of service on their lords, do well thrive by them. ... I AM NOT WHAT I AM"
(1.1.44-67)

Explanation:  to Iago tries to reassure Roderigo that he is actually pretending to be loyal to Othello so that he can eventually find a way to destroy his new marriage.