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Determine the possibility of recycling of supernatant solutions of Rhodamine B isothiocyanate (RBITC), which previously reacted with surface modified silica particles.

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i) A large part of the chromophore does not react with these particles and ii) that this compound is stable regardless of storage time. For this purpose, it is necessary to study whether the chromophore does not undergo any decomposition during the storage time or during the conjugation reaction with the surface of the particle.

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I. The synthesis of fluorescent particles of silica presented too many aggregates and polydispersity.
II. In relation to the recycling cycles observed in Optical Fluorescence Microscopy (MOF):
O The particles with the old RBITC, at all times presented almost no fluorescence, which refutes the hypothesis of
That this compound is stable, irrespective of storage time.
O The particles with the reaction of the fresh RBITC, in their 3 cycles, maintained the same intensity of fluorescence, so the hypothesis that much of the chromophore does not react with these particles is correct.

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The present investigation showed that the solutions of the RBITC with little storage time can be reused because this chromophore does not lose its fluorescence intensity.
This discovery is very useful for its correct use and could contribute to: reduce the expenses in the purchase of this compound; Take care of the environment by virtue of its toxicity; And optimize its use taking full advantage of its potential.

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Information about the cromophore, Empirical Formula (Hill Notation) C29H30ClN3O3S

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Rhodamine B is a chemical compound and a dye. It is often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers.