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I dont know the measurement😂

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I looove reading old books because they explain theoretical and methodology very detailed. Enjoy reading

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Fariza and Azizah, have you check Dr. Mashitah's research? What is your preference in sperm freezing?

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Fariza and Azizah, have you check Dr. Mashitah's research? What is your preference in sperm freezing?

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Cryoprotectant and cryopreservation of equine semen 

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Cryoprotectant and cryopreservation of equine semen 

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1. main advantage to using frozen semen in any breeding program is faster genetic gain for the inherited trait desired.
2. Fertility rates using frozen stallion spermatozoa are not equal to that of fresh semen
3. As equine semen is a one of the most difficult in the industry to cryopreserve efficiently without causing damage to the membrane or apoptosis.

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Hi all, lovely day. Can you find out if we can stain blastocyst with FdA. And which stage. Write in the comment. Thanks!

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Koon and Fariza, I am still waiting for your selfie...and finalize discussion with your pair.

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Koon and Fariza, I am still waiting for your selfie...and finalize discussion with your pair.

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Triton X 100
Hoechst
Propidium iodide

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1. There are two types of cryopreservation procedures: slow freezing and vitrification.
2. Usually storage time does not show significant effect to the fertility of the oocytes, but some studies show longer time may cause changing in quality of the oocytes hence affect the fertility.
3. Contamination may occur in liquid nitrogen due to it did not sterile.

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mendeley reference and there are lotsss more in youtube

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1. Use mature bulls
2. Crypopreserved sperm in liquid nitrogen do maintain population especially those of
endangered species.
3. Storage time does not show significant effect to the characteristics of spermatozoa.
4. Ejaculates must be evaluated for pH, volume, colour, viability, mobility, abnormality and sperm comcentration before amd after cryopeserved.
5. Inter-male variablity give impact to the susceptibility of bull sperm to cryoinduced damage.
6. Storage time divide into two: short term storage and long term storage
Short term : 0h, 24h,48h
Long term : 1 week, 1 month
7. Agree to use two different type of extenders
8. Should have one control sample,

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1. we are using mice or ruminant embryo for prolonged storage?
2. does assessment for embryo development within 48 H in every 4 H is somehow quite impossible ? to think that  i have to stay in lab  almost a week for this purpose :D

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2. Duration of  our mimic transport are 48/72/96 hours.
3. We are using embryo at cleavage stage.

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Transportation of embryo using liquid nitrogen 

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Transportation of embryo using liquid nitrogen 

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1. We agree to use open carrier instead of close carrier

2. Duration of storage to mimic transport : 48hrs/72hrs/96hrs

3. Not doing oocyte vitrification, in vivo embryo at cleavage stage will be collected and use as our sample.

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1. Stable stage of embryo for vitrification : morula
2. will do staining for blastocyst after thawing
3. open carrier for vitrification
4.will assess:
- survivality of embryo after thawing and during culture
-development of morula to blastocyst
5. minimizing as much parameters to be present
6. agree to use no vitrification and no cryopreserved morula for control.
7.time of cryopreserved is the parameter:
-immediately 0 H
-24 H
-168 H (7 days)
8. culture within 48 H and wil be assess for every 4 H
*can be alter depends on availability

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In agreement to use IVF and time as control, using chemicals as stimuli (eg: ethanol, 6-DMAP, A23187) (but chemicals TBC, as I need to further read on it) as varying factor.

If possible, will try to do freezing & staining

Question: is it confirmed that I'll be using superovulated mice as my sample?

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1. We decided to use open carrier as control for vitrification of cleavage stage embryo, but we are wonder open pulled straw is better or cryoloop?

2. Can we have different storage duration to mimic transport of embryo and then see the effect on developmental of embryo for each duration. ( eg. 48hrs/72hrs/96hrs)

3. We observe the developmental of blastocyst under fluorescence microscope after 48 hrs of thawing? Or longer than that?

4. We are using mice or goat?

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Suggest the best CPA for embryos and sperm

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Suggest the best CPA for embryos and sperm

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2. mendeley desktop
3. share document

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- crypreservation of sperm may lead to decrease in freezing viability and fertilization ability in animals
- the viability and motility of sperm gradually decrease over storage time
- abnormality gradually increases over time
- concentration does not significantly different during the period of storage

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1. vitrification is a convenient cryopreservation method, either open carrier and closed carrier have similar effect.
2. vapor nitrogen is a safety and newly developed transport method compare to liquid nitrogen.
3. concentration and the volume of cryoprotectant used with effect the survival rate of oocytes and embryo.

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1. Close carrier is better than open carrier during vitrification and storage of oocyte amd embryo as the risk of contamination is higher

2. Survival rate can be higher if the warming rate of carriers is higher.

3. Sterilization of LN2 using ultraviolet irradiation is too complicated and not preferrable (in my personal opinion)

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1. Principal step for the success of nuclear transfer for cloning is the efficient method of oocyte activation

2. There are various parthenogenetic activation

3. Different species will have different effect on different type of chemicals/stimuli/methods chosen

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1.vitrification of embryois influenced by concentration and combination of CPAs.
2.time of exposure during vitrification treatment is important to achieved osmotic equilibrium.
3.cell injury will occur during prolonged storage but no significant damage as embryo will restored after 24H of culture.