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I learned about the differences in time it takes to complete the stages of mitosis (prophase, metaphase, anaphase, and telophase) in comparison to one another. I am still wondering what the actual proportions of times are. I think i did somewhat well, but I was a little subjective in the cells that I chose and counted. I think, as a group, we did relatively well because we had around the same numbers as the class averages.

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Part of our hypothesis was correct. We determined that interphase takes the longest time (455.76 minutes) to complete. This makes sense because, during interphase, the cell has to grow and duplicate its DNA. This takes a long time, and the cell has to continuously check (checkpoints) on the DNA to make sure it has not mutated. The shortest (25.92 minutes) was telophase at which point the nuclei of the daughter cells are being formed. This portion of mitosis sometimes includes cytokinesis.

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Assuming that mitosis takes 720 minutes to complete and the percentage of cells in a phase is equal to its percentage of the time for mitosis.

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Under a microscope

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Interphase- consists of G1, S, and G2 stages. DNA is replicated, the cell grows, and prepares for mitosis.
Prophase- chromosomes condense, spindle is formed, and nuclear envelope breaks down.
Metaphase- chromosomes line up at metaphase plate, kinetochores attach to microtubules.
Anaphase- chromosomes are pulled apart by microtubules and the use of motor proteins.
Telophase/cytokinesis- spindle is broken down, nuclei of daughter cells form, cells begin to pinch off (animal) or a cell plate forms (plant).

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Based on class averages

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Our group is #3

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Materials:
-onion root slide
-microscope
Set up:
1. place the slide on the microscope and adjust into focus.
2. move the slide around until you can see the cells close the the very tip, but not actually at the tip.(these will be the best source of mitosis.)
3. Once you can see the cells clearly, start to name the stage of mitosis they are in. (It is best to go in columns or rows to get accurate numbers.) Your partner should tally how many cells you have counted and what phase they are in.
4. Once you have counted 50 cells, switch, repeating steps 1-3 on a different root. (There are 3 samples per slide.)

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Our group believed that interphase would be the longest, then metaphase, then prophase, then telophase, and finally anaphase would be the shortest.

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How long do each of the phases of mitosis (including interphase) take?