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References!

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Explain techniques: CRISPR-Cas9, blue white screening, plasmid isolation.
Theory about: lacZ gene, B-gal

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Result of blue/white screening (colour of colonies on the plates).

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Result of blue/white screening (colour of colonies on the plates).

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DNA concentrations measured on nanodrop.

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DNA concentrations measured on nanodrop.

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Did we get the results that were expected- why not?

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How was the function of lacZ tested using CRISPR.

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How was the function of lacZ tested using CRISPR.

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Nanodroplet A260/A280 ratio

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Future steps

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Crispr-cas: background, discovered as a bacterial adaptive immune system.

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Explanation of different phenotypes observed in dishes A-D

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pLZDonor (control)
pLZDonorGuide

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Colour of colonies the first and second week

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Colour of colonies the first and second week

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Nanodrop: Nevne konsentrasjon og tetthet, dette er ikke hovedfokuset i modulen. 

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Nanodrop: Nevne konsentrasjon og tetthet, dette er ikke hovedfokuset i modulen. 