# data[].attributes.headline
	
	
		
			Mahendra Kumar Trivedi Treating Chronophobia Naturally
		
	

# data[].attributes.subject
	
	
		
			Mahendra Kumar Trivedi Treating Chronophobia Naturally
		
	

# data[].attributes.body
The Trivedi Effect is a unique human biofield energy possessed by Mahendra Trivedi that is very effective in treating Chronophobia or fear of future, naturally.

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Future | Energy Transmission | Trivedi Testimonials

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Douglas shares impact of Trivedi Effect in his life. After the Energy Transmission he feels a greater level of happiness in his life. The Trivedi Effect has improved his daily life and his driving habits. Since he is with Mahendra Trivedi and The Trivedi Effect, he doesn't get angry.

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			Mahendra Trivedi – Natural Treatment for Enlarged Prostate
		
	

# data[].attributes.subject
	
	
		
			Mahendra Trivedi – Natural Treatment for Enlarged Prostate
		
	

# data[].attributes.body
Mahendra Trivedi possesses a very natural and effective healing power called The Trivedi Effect. Watch full video to know about its numerous benefits.

# data[].attributes.attachment_link.entities[].name
Enlarged Prostate Herbal Remedies & Natural Treatment for BPH

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Endosterol Health Menopause Bph Home Remedies Prostate Natural Remedies Female Labido

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Financial and Health Stability | Trivedi Testimonials

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Ayurvedic Treatment of Enlarged Prostate

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Overcome from Benign Prostatic Hyperplasia (BPH) | Trivedi Testimonials

# data[].attributes.attachment_link.description
Padman Pillai shares his experience about the Energy Transmission. He was suffering from BPH due to this problem, urination was very painful & difficult for him. But after the transmission his problem was totally resolved and he has prosperity in all areas of the life. To know more, visit: http://www.triveditestimonials.com/video-testimonials/health/

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			Mahendra Trivedi – How to Empower & Connect to the Divine
		
	

# data[].attributes.subject
	
	
		
			Mahendra Trivedi – How to Empower & Connect to the Divine
		
	

# data[].attributes.body
Watch full video to understand how Mahendra Kumar Trivedi’s powerful spiritual forcefield can help you connect to the divine.

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Trivedi Effect on Relationships & Personal Life | Energy Transmission | Trivedi Testimonials

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Healers Mastery Program | Trivedi Effect | Trivedi Testimonials

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Connection to the divine with The Trivedi Effect | Trivedi Testimonials

# data[].attributes.attachment_link.description
Jeff Peoples talks about the connection with the Divine. He says that Trivedi Effect has helped him to connect with God of his understanding. He says, pay attention on how miraculous God is, and hope the right miracle happen in your life.

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			Mahendra Trivedi – Natural Remedies for Depression & Anxiety
		
	

# data[].attributes.subject
	
	
		
			Mahendra Trivedi – Natural Remedies for Depression & Anxiety
		
	

# data[].attributes.body
The Trivedi Effect is a unique energy treatment by Mahendra Trivedi that has been scientifically proven to heal Depression & Anxiety naturally.

# data[].attributes.attachment_link.entities[].name
Trivedi Master Blessings | Improved Business & Financial Wellness

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Life | Energy Transmissions | Trivedi Testimonials

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Trivedi Water on Health | Trivedi Testimonials

# data[].attributes.attachment_link.title
Impact of Trivedi Effect on Physical and Mental Health | Trivedi Testimonials

# data[].attributes.attachment_link.description
Faith has invested in properties and it didn't work according to her expectations. Due to this she was suffering from stress, deep depression and anxiety. She joined Master Healers Programs in 2011. As an impact of Trivedi Effect she feels more energized and has noticed positive physical and mental changes.

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			An Experimental Study on Translational Medicine |Omicsonline
		
	

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			An Experimental Study on Translational Medicine |Omicsonline
		
	

# data[].attributes.body
An experimental study on Translational medicine, it is a discipline within biomedical and public health research that aims to improve the health of individuals and the community by "translating" findings into diagnostic tools, medicines, procedures, policies and education.

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			An Experimental Study on Translational Medicine |Omicsonline
		
	

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			An Experimental Study on Translational Medicine |Omicsonline
		
	

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			Biofield & Its Effect on Enterobacter aerogenes
		
	

# data[].attributes.subject
	
	
		
			Biofield & Its Effect on Enterobacter aerogenes
		
	

# data[].attributes.body
Enterobacter aerogeneshas been commonly described as a versatile opportunistic pathogen in hospital infections. Study this research to know about biofield impact on E. aerogenes.

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Volume 5 * Issue 3 * 1000155

ISSN: 2161-1025 TM, an open access journal

Trivedi et al., Transl Med (Sunnyvale) 2015, 5:3

http://dx.doi.org/10.4172/2161-1025.1000155

Research ArticleOpen Access

Characterization of Phenotype and Genotype of Biofield Treated

Mahendra Kumar Trivedi1 , Alice Branton1 , Dahryn Trivedi1, Gopal Nayak1, Mayank Gangwar2 and Snehasis Jana2*

1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd, Bhopal-462026, Madhya Pradesh, India

Enterobacter aerogenes (E. aerogenes) has been commonly described as a versatile opportunistic pathogen in

hospital infections. The aim of the present work was to evaluate the impact of bioeld treatment on E. aerogenes for

its phenotypic and genotypic characteristics. E. aerogenes bearing ATCC 13048 (American Type Culture Collection)

was procured from Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group

was subjected to Mr. Trivedi's bioeld treatment and analyzed for antimicrobial susceptibility, minimum inhibitory

concentration (MIC), biochemical reactions, and biotype using automated MicroScan Walk-Away® system. In addition,

treated group of E. aerogenes was evaluated for DNA polymorphism by Random Amplied Polymorphic DNA (RAPD)

and 16S rDNA sequencing to establish the phylogenetic relationship of E. aerogenes with different closely related

bacterial species. Antimicrobial susceptibility results showed an alteration of 14.28% among twenty-eight tested

antimicrobials. Similarly, 15.65% tested antimicrobials showed an alteration in MIC values. Chloramphenicol showed

improved sensitivity i.e. resistant to susceptible after bioeld treatment, with the support of decreased MIC by two

to control. Bioeld treatment showed an impact on biochemical reactions (9.09%) followed by a change in biotype

number (7770 5272) in treated group with respect to control (7770 5372). Using RAPD analysis, sample showed an

average range of 4 to 42% of polymorphism, while 16S rDNA study showed that treated sample was detected as

Kluyvera cryocrescens (GenBank Accession Number: AM184245) with 97% identity of gene sequencing data, which

was nearest homolog species to Enterobacter aerogenes strain: C1111 (Accession No. AB244467). These results

suggest that Mr. Trivedi's unique bioeld treatment can alter the antimicrobial sensitivity pattern, thus it can be used as

alternate energy medicine in future.

Polymorphism; RAPD; 16S rDNA analysis

Enterobacter aerogenes (E. aerogenes) is a common organism of

most of the hospital-acquired infections. Adaptive capability of E.

aerogenes is remarkable and can easily acquire resistance to against

β-lactam antibiotics [1]. During last ve years, it has shown that E.

aerogenes isolates are having natural resistance against aminopenicillins,

and express an extended-spectrum β-lactamase (ESBL), which results

resistance to β-lactam antibiotics [2]. However, E. aerogenes exhibits

acquired resistance against dierent categories of antimicrobial agents.

General resistance mechanisms exhibited by E. aerogenes involves

membrane permeability, p-glycoprotein eux pump, and enzyme

degradation against carbapenems, uoroquinolones, quinolones,

tetracycline, and chloramphenicol [3]. Currently, colistin, and

polymyxin antibiotic have been preferred as alternative drugs against

Gram-negative pathogens, due to the extended resistance of Gram-

negative bacteria against almost all antibiotics [4]. Recently, colistin in

antibiotic therapy shows serious toxicity and associated adverse eects

like neurotoxicity and nephrotoxicity [5]. Because of all the associated

side eects and failure of drug treatment therapy, alternate treatment

approach is required. Recently, an alternate treatment is known as

bioeld energy and is reported that inhibits the growth of bacterial

Bioeld is the name given to the electromagnetic eld that

permeates and surrounds living organisms [7]. It is referred as

biologically produced electromagnetic and subtle energy eld that

provides regulatory and communication functions within the human

organism. Various internal physiological processes such as blood ow,

brain, heart function, etc. that generate bioeld. Biomagnetic elds

around the human body can be measured using dierent techniques

[8]. Researchers have attempted dierent biological studies and eects

*Corresponding author: Jana S, Trivedi Science Research Laboratory Pvt. Ltd.,

Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd, Bhopal-462026,

Received August 15, 2015; Accepted September 02, 2015; Published September

Characterization of Phenotype and Genotype of Bioeld Treated Enterobacter

Copyright: © 2015 Trivedi MK, et al., This is an open-access article distributed

under the terms of the Creative Commons Attribution License, which permits

unrestricted use, distribution, and reproduction in any medium, provided the

original author and source are credited.

of bioeld on various biomolecules such as proteins, antibiotics [9],

and conformational change in DNA [10], bacterial cultures [11] etc.

us, it can be concluded that human can to harness the energy from

environment or universe and can transmit into any living or nonliving

object(s) around the Universe. e objects always receive the energy

and responding to useful way that is called bioeld energy. Mr.

Trivedi's unique bioeld treatment has been well known and studied

in the eld of material science [12-14], agricultural science [15-17],

and biotechnology [18]. Mr. Trivedi's unique bioeld treatment is

also known as e Trivedi Eect®, which was also reported in altering

the susceptibility of antimicrobials against pathogenic and multidrug

resistant microbes [19-21].

Aer consideration of the clinical signicance of E. aerogenes and

signicant impact of Mr. Trivedi's bioeld treatment on microbes,

authors evaluated the eect of bioeld treatment on E. aerogenes

followed by genotyping of treated organism. Genotyping was

performed using strain dierentiation and distinctive polymorphism

using polymerase chain reaction (PCR) technique of random amplied

polymorphic DNA analysis (RAPD) and 16S rDNA sequencing.

Volume 5 * Issue 3 * 1000155

ISSN: 2161-1025 TM, an open access journal

Enterobacter aerogenes ATCC 13048 [American Type Culture

Collection] was procured from Bangalore Genei, in sealed pack, and

stored as per the recommended storage conditions for further use.

e anti-microbial susceptibility, biochemical reactions, and biotype

number were evaluated on MicroScan Walk-Away® (Dade Behring Inc.,

West Sacramento, CA) using Negative Breakpoint Combo 30 (NBPC

30) panel. DNA ngerprinting (RAPD) and the 16S rDNA sequencing

studies were carried out using Ultrapure Genomic DNA Prep Kit;

Cat KT 83 (Bangalore Genei, India). All the tested antimicrobials,

biochemicals and other reagents were procured from Sigma-Aldrich,

India.

Study design and bioeld treatment

E. aerogenes strain was divided into two groups i.e. control and

treatment. e treatment group was in sealed pack and handed over

to Mr. Trivedi for bioeld treatment under laboratory conditions. Mr.

Trivedi provided the treatment through his energy transmission process

to the treated group without touching the sample. Aer treatment,

control and treated groups were assessed on day 10 for antimicrobial

susceptibility, minimum inhibitory concentration (MIC), biochemical

reactions, biotype, and genotyping using RAPD and 16S rDNA analysis.

e results of treated samples were compared with respect to control.

Investigation of antimicrobial susceptibility assay

Investigation of antimicrobial susceptibility of E. aerogenes was

carried out with the help of automated instrument, MicroScan Walk-

Away® using Negative Breakpoint Combo 30 (NBPC30) panel as per

the manufacturer's instructions. e panel was allowed to equilibrate

to room temperature prior to rehydration. All opened panel were used

on the same day. e tests were carried out on MicroScan, which were

miniaturized of the broth dilution susceptibility test that has been

dehydrated. Briey, 0.1 mL (100 μL) of the standardized suspension of

E. aerogenes was pipetted into 25 mL of inoculum water using pluronic

and inverted 8-10 times and inoculated, rehydrated, and then subjected

to incubation for 16 hours at 35°C. Rehydration and inoculation was

performed using the RENOK® system with inoculators-D (B1013-4).

e detailed experimental procedures and conditions were followed

as per the manufacturer's instructions. Briey, aer inoculation and

rehydration with a standardized suspension of E. aerogenes, it was

incubated at 35°C for 16 hours. MIC and a qualitative susceptibility

like susceptible (S), intermediate (I), inducible β-lactamases (IB), and

resistant (R) were determined by observing the lowest antimicrobial

concentration showing growth inhibition [22].

were determined by MicroScan Walk-Away® system, it interprets

the microbe biochemical results with the use of a photometric or

uorogenic reader. On the basis of nature of bacilli (Gram-negative or

Gram-positive), it generates computerized reports using conventional

panels, which utilizes the photometric reader and provide identication

results. Before commencing the experiment, the NBPC 30 panel was

rst incubated and read on the MicroScan Walkaway system. Aer

completion of reading on the Walkaway system, the NBPC 30 panel was

removed from system and read on the Biomic system within 1 hour.

MicroScan Walk-Away instrument consist of a database associated

with collective information, which was required to identify the group,

genera, or species of the family. Detailed experimental procedure was

followed as per manufacturer-recommended instructions [22].

Biotype number: e biotype number of E. aerogenes was

determined by MicroScan Walk-Away® processed panel data utilizing

data of biochemical reactions. Similar experimental procedure

was followed for identication of biotype number as described in

biochemical reaction study, and as per manufacturer-recommended

Random Amplied Polymorphic DNA (RAPD) analysis

ree series of inoculums (one for control and other two for

treatment named as treatment A and B) were prepared from E. aerogenes

sample. Two inoculums (treatment samples A and B) were subjected

to Mr. Trivedi's bioeld treatment. Whilst handing over treated groups

to Mr. Trivedi for bioeld treatment, optimum precautions were taken

to avoid the contamination. Aer that, the treated samples (A and B)

were sub-cultured by taking 1% inoculum and inoculated to fresh 5 mL

medium and labeled as treatment A-1 and treatment B-1 respectively.

Control and treated samples were incubated at 37°C with 160 rpm for

18 h. Subsequently, the cultures were spun down, and genomic DNA

was isolated for control and treated samples using Genomic DNA Prep

Kit (Bangalore Genei, India). Designed primers were initially screened

for their sharpness, further primers that have the basic of sharpness,

clarity of the prole and the existence of polymorphisms were chosen

for further study. RAPD was performed with all samples of E. aerogenes

using ve RAPD primers, which were labelled as RBA8A, RBA13A,

RBA20A, RBA10A and RBA15A. e PCR mixture contained 2.5 μL

each of buer, 4.0 mM each of dNTP, 2.5 μM each of primer, 5.0 μL each

of genomic DNA, 2U each of Taq polymerase, 1.5 μL of MgCl2 and 9.5

μL of nuclease-free water in a total of 25 μL mixture. PCR amplication

protocol followed with initial denaturation at 94ºC for 7 min, followed

by 8 cycles of denaturation at 94ºC for 1 min, annealing at 35ºC for 1

min, and extension at 72ºC for 2 min; and 35 cycle of denaturation at

94ºC for 1 min, annealing at 38ºC for 1 min, and extension at 72ºC

for 1.5 min; and the nal extension at 72ºC for 7 min. Amplied PCR

products (12 μL) from all samples (control and treated) were separated

on 1.5% agarose gels at 75 volts, stained with ethidium bromide and

visualized under UV illumination [23].

e percentage of polymorphism was calculated using following

Percent polymorphism = A/B×100;

Where,A = number of polymorphic bands in treated sample; and B

= number of polymorphic bands in control.

Amplication and gene sequencing of 16S rDNA

Genomic DNA was isolated and puried from a treated group

of E. aerogenes cells by using genomic purication Kit, as per the

manufacturer's instructions. e PCR product was bi-directionally

sequenced using the forward, reverse, and an internal primer. DNA 16S

region amplication was performed using the primer set 16S forward and

reverse primer [24]. 16S rDNA gene (~1.5 kb) was amplied by universal

primers; forward primer (5ˊ -AGAGTTTGATCCTGGCTCAG-3ˊ )

and reverse primer (3ˊ -ACGGTCATACCTTGTTACGACTT-5ˊ ).

Amplication was carried out in a Rapid Cycler thermocontroller, with

initial denaturation, annealing and extension temperature. Following

amplication products were analyzed by gel electrophoresis at 100 V

(in 1.0% agarose gel, 0.2 µg of ethidium bromide mL-1) in tris-acetate

buer (TAE), and visualized under UV light in a gel documentation

unit (BioRad Laboratories, USA). e amplied fragment of PCR was

puried from the agarose gel by DNA Gel Extraction Kit. Sequencing of

amplied product was carried out on a commercial basis from Bangalore

Genei, India. e obtained 16S rDNA sequences data were aligned

and compared with the sequences, available in GenBank database

of National Center for Biotechnology Information (NCBI) using

the algorithm BLASTn program. e multiple sequence alignment/

Volume 5 * Issue 3 * 1000155

ISSN: 2161-1025 TM, an open access journal

phylogenetic tree were constructed using MEGA 3.1 soware using

neighbor-joining method [25].

Assessment of antimicrobial susceptibility

e eect of bioeld treatment on E. aerogenes with respect to

antimicrobials susceptibility pattern and MIC are summarized in Tables

1 and 2, respectively. e data were analyzed and compared with respect

to control. e treated cells of E. aerogenes showed 14.28% alteration out

of twenty-eight tested antimicrobials as compared to control. Results

showed altered antibiogram of cefotaxime, cefotetan, chloramphenicol,

and piperacillin as compared to control. Cefotaxime was converted from

inducible β-lactamases to intermediate, while cefotetan changed from

inducible β-lactamases to resistant. Chloramphenicol resistance prole

was improved from resistant to susceptible as compared to control.

Piperacillin sensitivity was also altered from inducible β-lactamases

to intermediate. Rest of the tested antimicrobials did not show any

alteration in sensitivity with respect to control.

Similarly, sensitivity results of tested antimicrobials are well

supported with MIC results (Table 2). Cefotaxime showed four folds

alteration in MIC value (32 µg/mL) while cefotetan showed two folds

change in MIC value (>32 µg/mL) with respect to control. Improved

sensitivity of chloramphenicol also showed decreased MIC value by two

folds (i.e. >16 to ≤8 µg/mL) as compared to control. Noroxacin also a

showed decrease in MIC value by two folds aer bioeld treatment with

2Amoxicillin/k-clavulanate R R

3Ampicillin/sulbactam I I

23 Piperacillin/tazobactam IB IB

26 Ticarcillin/k-clavulanate IB IB

28 Trimethoprim/sulfamethoxazole S S

R: Resistant; I: Intermediate; S: Susceptible; '-': IB: Inducible β-lactamases; deleted

Table 1: Effect of bioeld treatment on multidrug resistant lab isolates of

Enterobacter aerogenes to antimicrobial susceptibility.

respect to control (i.e. 8 to ≤4 µg/mL). However, piperacillin showed

four folds change in MIC value (i.e. ≤16 to 64 µg/mL) as compared

to control. Overall, 15.65% tested antimicrobials showed altered MIC

values with respect to control. e rest of the tested antimicrobials did

not show any alteration in MIC values with respect to control data.

According to National health statistics reports of National Center

for Health Statistics in the Unites States, 2002-2012, bioeld treatment as

an alternative energy medicine was well reported and practiced among

adults [26]. e current experiment was designed todemonstrate

the impact of bioeld treatment on E. aerogenes for its susceptibility

pattern, biochemical reaction, and biotype number. Further, molecular

methods were performed to study the genetic alterations and similarities

using RAPD and 16S rDNA sequencing methods. Increased emergence

of resistant E. aerogenes is a global health problem, as an emerging

Gram-negative pathogen in Enterobacteriaceae family, associated with

severe hospital acquired infections. E. aerogenes isolates have a broad

ability to develop antimicrobial resistance [27]. Results showed, bioeld

treatment has induced change in susceptibility pattern of antimicrobials

such as cefotaxime, cefotetan, chloramphenicol, and piperacillin as

compared to control. Chloramphenicol showed improved sensitivity

aer bioeld treatment from resistant to susceptible. Chloramphenicol

exhibited natural resistance against E. aerogenes, and it was mediated

2Amoxicillin/K-clavulanate>16/8 >16/8

3Ampicillin/sulbactam16/8 16/8

27 Piperacillin/tazobactam≤16 ≤16

30 Ticarcillin/k-clavulanate≤16 ≤16

32 Trimethoprim/sulfamethoxazole ≤2/38≤2/38

Table 2: Minimum inhibitory concentration (MIC) oftested antimicrobials against

MIC values are presented in μg/mL; ESBL: Suspected extended-spectrum

# data[].attributes.attachment_link.title
Characterization of Phenotype and Genotype of Biofield Treated Enterobacter aerogenes - PdfSR.com

# data[].attributes.attachment_link.description
Enterobacter aerogenes (E. aerogenes) has been commonly described as a versatile opportunistic pathogen in hospital infections. The aim of the present work was to evaluate the impact of biofield treatment on E. aerogenes for its phenotypic and genotypic characteristics. E.

# data[].attributes.headline
	
	
		
			Human Biofield Energy Treatment on Enterobacter aerogenes
		
	

# data[].attributes.subject
	
	
		
			Human Biofield Energy Treatment on Enterobacter aerogenes
		
	

# data[].attributes.body
Tests to reveal the impact of human biofield energy treatment on Enterobacter aerogenes for its phenotypic and genotypic characteristics.

# data[].attributes.attachment_link.keywords[]
phenotype and genotype of enterobacter aerogenes

# data[].attributes.attachment_link.title
"Characterization of Phenotype and Genotype of Biofield Treated Enterobacter aerogenes" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Enterobacter aerogenes (E. aerogenes) has been commonly described as a versatile opportunistic pathogen in hospital infections. The aim of the present work was to evaluate the impact of biofield treatment on E. aerogenes for its phenotypic and genotypic characteristics. E.

# data[].attributes.headline
	
	
		
			Characterization of Biofield Treated Enterobacter aerogenes
		
	

# data[].attributes.subject
	
	
		
			Characterization of Biofield Treated Enterobacter aerogenes
		
	

# data[].attributes.body
The study suggest that the biofield energy treatment might be used as an alternate treatment approach in future than the existing antimicrobial therapy.

# data[].attributes.headline
	
	
		
			Properties of Phenotype & Genotype of Enterobacter aerogenes
		
	

# data[].attributes.subject
	
	
		
			Properties of Phenotype & Genotype of Enterobacter aerogenes
		
	

# data[].attributes.body
The study results suggest that human biofield energy treatment can alter the antimicrobial sensitivity pattern, thus it can be used as alternate energy medicine in future.

# data[].attributes.headline
	
	
		
			Biofield | Phenotype and Genotype of Enterobacter aerogenes
		
	

# data[].attributes.subject
	
	
		
			Biofield | Phenotype and Genotype of Enterobacter aerogenes
		
	

# data[].attributes.body
Authors evaluated the effect of biofield on E. aerogenes followed by genotyping of treated organism. Genotyping was performed using strain differentiation and distinctive polymorphism using PCR technique of random amplified polymorphic DNA analysis (RAPD) and 16S rDNA sequencing.

# data[].attributes.attachment_link.title
Characterization of Phenotype and Genotype of Biofield Treated Enterobacter aerogenes

# data[].attributes.attachment_link.description
Enterobacter aerogenes (E. aerogenes) has been commonly described as a versatile opportunistic pathogen in hospital infections. The aim of the present work was to evaluate the impact of biofield treatment on E. aerogenes for its phenotypic and

# data[].attributes.headline
	
	
		
			Phenotype & Genotype of Biofield Treated Enterobacter aerogenes
		
	

# data[].attributes.subject
	
	
		
			Phenotype & Genotype of Biofield Treated Enterobacter aerogenes
		
	

# data[].attributes.body
The aim of the present work was to evaluate the impact of biofield energy treatment on E. aerogenes for its phenotypic and genotypic characteristics.