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“Here’s the smell of the blood still. All the perfumes of Arabia will not sweeten this little hand,” (Shakespeare 5.1.53-55).

Clearly, Lady Macbeth is haunted by the image of the blood on her hands after helping Macbeth get away with Duncan’s murder. However, this blood that is on her hands could also symbolize the immense guilt that she feels for tempting her husband into killing Duncan. Lady Macbeth being torn apart with her guilt is another message from Shakespeare that regicide has horrible consequences.

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Rather than so, come fate into the list,
And champion me to th' utterance. (Shakespeare 3.1.76-77)

This quote connects to the EQ of fate vs. free will because Macbeth is now willing to challenge fate itself, even though the prophecies made him king. If Macbeth is able to kill Banquo and his offspring, then this proves that humans exert much control over their own free will, but if the prophecies are true, then humans have little to no control of their own lives. This is probably going to be the biggest confrontation between fate and free will, and from there it can be determined if one exerts control over their own life or if an external source controls your will.

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“Another yet? A seventh? I’ll see no more.
And yet the eighth appears who bears a glass
Which shows me many more, and some I see
That twofold balls and treble scepters carry” 

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“Another yet? A seventh? I’ll see no more.

And yet the eighth appears who bears a glass

Which shows me many more, and some I see

That twofold balls and treble scepters carry” (Shakespeare 4.1 117-121). This procession comes from the witches, and is therefore fate. The idea that it is destiny for Banquo's sons to be Kings emphasizes the legitimacy of King James' kingship, and this motif is making it seem like it was inevitable and rightful for Banquo's children to be Kings.

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“My hands are of your color, but I shame

To wear a heart so white” (Shakespeare 2.3.64-65)

Macbeth was unable to bring himself to see Duncan’s dead body once he has killed him, so Lady Macbeth went and cleaned up the crime scene for him. After coming back and seeing how devastated Macbeth is, she shames him for being mentally weaker than her. She stresses that she too, was guilty of the crime, as the blood on her hands suggests, but she was not frightened like Macbeth was. This quote demonstrates the expectation of traditional gender roles, as Lady Macbeth shames Macbeth for not having the courage that he, as a man, should have. She also makes the comparison of him to herself, further demonstrating his cowardice by showing that a woman, who was supposed to be weak and easily intimidated, was more courageous than him.

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“Come, you spirits that tend on moral thoughts, unsex me here, and fill me from the crown to the toe top-full of direst cruelty. Make thick my blood." (1.5 47-50)
Blood is shown as the characteristics to a person, almost as their connection to humanity or a reflection of their morals. Through Lady Macbeth asking for the spirits to make her blood thick, she is asking to become less empathetic towards the murder of Duncan.

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“Glamis hath murdered sleep, and therefore

Cawdor. Shall sleep no more. Macbeth shall sleep no more” (Shakespeare 39-41). This shows up in the text after MacBeth killed Duncan and is starting to reflect on what he just did.

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"My hands are of your color but I shame to wear a heart so white. I hear knocking At the south entry. Retire we to our chamber. A little water clears us of this deed. How easy it is then" (82-87 Shakespeare)! Blood as a motif is seen several times in the book all ready as Macbeth is first introduced to the reader and as he murders the king with his wife. The motif blood is used in this instance for Macbeth's wife to show that what she has done is for Macbeth and that she isn't afraid to show his color unlike him with his unwillingness to go place the daggers on the kings guards. Blood represents the significance of events like when in the hospital the blood on the Sargant body represents the rise of Macbeth and his power then the down fall of his morality when he murders the king. Blood could also represent the guilt Macbeth has for his action for example when he kills the king.

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“‘Glamis hath murdered sleep, and therefore

Cawdor

Shall sleep no more. Macbeth shall sleep no more’”(2.2 55-57).

Here it is saying that because he murdered Duncan, he will not be allowed to sleep anymore.

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"With all great Neptune's ocean wash this blood Clean from my hand? No, this my hand will rather the multitudinous seas incarnadine, Making the green one red" (Shakespeare 2.2.2.63-66).
The motif blood appears many times as Macbeth has just killed King Duncan. So blood is not only here to show the blood that is shed from King Duncan's death, but also of the guilt that Macbeth faces from killing him. Here Macbeth asks the ocean to clean his hands, rid him of his guilt, but he knows what's done is done. King Duncan is now dead.

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"Methought I heard a voice cry 'Sleep no more! Macbeth does murder sleep'-the innocent sleep, Sleep that knits up the raveled sleave of care, The death of each day's life, sore labor's bath, Balm of hurt minds, great nature's second course, Chief of nourisher in life's feast" (2.2.47-52).
This motif of sleep appears in a monologue once Macbeth had completed the deed of killing King Duncan. It foreshadows the madness to come, as one without the tranquility of sleep becomes mad. This motif contributes to the emerging theme of the consequences and guilt that corresponds with ambition.