# data[].attributes.attachment_link.readable_text
Researchers at the University of Pennsylvania have developed the first placenta-on-a-chip that can fully model the transport of nutrients across the placental barrier.

The flash-drive-sized device contains two layers of human cells that model the interface between mother and fetus. Microfluidic channels on either side of those layers allow researchers to study how molecules are transported through, or are blocked by, that interface.

Like other organs-on-chips, such as ones developed to simulate lungs, intestines and eyes, the placenta-on-a-chip provides a unique capability to mimic and study the function of that human organ in ways that have not been possible using traditional tools.

Research on the team's placenta-on-a-chip is part of a nationwide effort sponsored by the March of Dimes to identify causes of preterm birth and ways to prevent it. Prematurely born babies may experience lifelong, debilitating consequences, but the underlying mechanisms of this condition are not well understood due in part to the difficulties of experimenting with intact, living human placentae.

The research was led by Dan Huh, the Wilf Family Term Assistant Professor of Bioengineering in Penn's School of Engineering and Applied Science, and Cassidy Blundell, a graduate student in the Huh lab. They collaborated with Samuel Parry, the Franklin Payne Professor of Obstetrics and Gynecology; Christos Coutifaris, the Nancy and Richard Wolfson Professor of Obstetrics and Gynecology in Penn's Perelman School of Medicine; and Emily Su, assistant professor of obstetrics and gynecology in the Anschutz Medical School of the University of Colorado Denver.

The study was published in the journal Lab on a Chip.

The researchers' placenta-on-a-chip is a clear silicone device with two parallel microfluidic channels separated by a porous membrane. On one side of those pores, trophoblast cells, which are found at the placental interface with maternal blood, are grown. On the other side are endothelial cells, found on the interior of fetal blood vessels. The layers of those two cell types mimic the placental barrier, the gatekeeper between the maternal and fetal circulatory systems.

"That barrier," Blundell said, "mediates all transport between mother and fetus during pregnancy. Nutrients, but also foreign agents like viruses, need to be either transported by that barrier or stopped."

"One of the most important function of the placental barrier is transport," Huh said, "so it's essential for us to mimic that functionality."

In 2013, Huh and his collaborators at Seoul National University conducted a preliminary study to create a microfluidic device for culturing trophoblast cells and fetal endothelial cells. This model, however, lacked the ability to form physiological placental tissue and accurately simulate transport function of the placental barrier.

In their new study, the Penn researchers have demonstrated that the two layers of cells continue to grow and develop while inside the chip, undergoing a process known as "syncytialization."

"The placental cells change over the course of pregnancy," Huh said. "During pregnancy, the placental trophoblast cells actually fuse with one another to form an interesting tissue called syncytium. The barrier also becomes thinner as the pregnancy progresses, and with our new model we're able to reproduce this change.

"This process is very important because it affects placental transport and was a critical aspect not represented in our previous model."

The Penn team validated the new model by showing glucose transfer rates across this syncytialized barrier matched those measured in perfusion studies of donated human placentae.

While useful in providing this type of baseline, donated placental tissue can be problematic for doing many of the types of studies necessary for fully understanding the structure and function of the placenta, especially as it pertains to diseases and disorders.

"The placenta is arguably the least understood organ in the human body," Huh said, "and much remains to be learned about how transport between mother and fetus works at the tissue, cellular and molecular levels. An isolated whole organ is an not ideal platform for these types of mechanistic studies."

"Beyond the scarcity of samples," Blundell said, "there's a limited lifespan of how long the tissue remains viable, for only a few hours after delivery, and the system that is used to perfuse the tissue and perform transport studies is complex."

While the placenta-on-a-chip is still in the early stages of testing, researchers at Penn and beyond are already planning to use it in studies on preterm birth.

"This effort," Parry said, "was part of the much larger Prematurity Research Center here at Penn, one of five centers around the country funded by the March of Dimes to study the causes of preterm birth. The rate of preterm birth is about 10 to 11 percent of all pregnancies. That rate has not been decreasing, and interventions to prevent preterm birth have been largely unsuccessful."

As part of a $10 million grant from the March of Dimes that established the Center, Parry and his colleagues research metabolic changes that may be associated with preterm birth using in vitro placental cell lines and ex vivo placental tissue. The grant also supported their work with the Huh lab to develop new tools that could model preterm birth-associated placental dysfunction and inform such research efforts.

"Since publishing this paper," Samuel Parry said, "we've reached out to the principal investigators at the other four March of Dimes sites and offered to provide them this model to use in their experiments."

"Eventually," Huh said, "we hope to leverage the unique capabilities of our model to demonstrate the potential of organ-on-a-chip technology as a new strategy to innovate basic and translational research in reproductive biology and medicine."

The research was supported by the March of Dimes Prematurity Research Center at the University of Pennsylvania and the National Institutes of Health Director's New Innovator Award 1DP2HL127720-01.

Also contributing to the study were Huh lab members Emily Tess and Ariana Schanzer.

# data[].attributes.attachment_link.description
Researchers at the University of Pennsylvania have developed the first placenta-on-a-chip that can fully model the transport of nutrients across the placental barrier. The flash-drive-sized device contains two layers of human cells that model the interface between mother and fetus.

# data[].attributes.body
Drugs are categorized based on risk to the fetus.  Many drugs are placed in Category C; meaning not enough research has been done to determine if the drugs are safe.  It is currently unknown how the placenta allows some molecules and not others.  There are several drugs that are medically necessary for pregnant mothers but pose a risk to the fetus, thereby placing the mother at risk for medical complications.

# data[].attributes.headline
Drug Testing Shake Up with Organs-on-a-Chip

# data[].attributes.subject
Drug Testing Shake Up with Organs-on-a-Chip

# data[].attributes.body
Bloomberg
Published on Jul 29, 2015

# data[].attributes.attachment_link.title
Drug Testing Shake Up with Organs-on-a-Chip

# data[].attributes.attachment_link.description
July 28 -- Our latest BWest series: Bioengineering the Senses. We take a closer look at tech on the frontiers of medical science. Today, we take a look at organs-on-a-chip under development at the Wyss Institute of Harvard University. Could this device speed up and improve the way new medicines are developed?

# data[].attributes.body
This technology would be extremely helpful assessing drug safety for pregnant women. This is especially helpful to those who are taking medications that are medically necessary, keeping both mother and baby safe from harm.
This research is just one of the many doors being opened for 'organs-on-a-chip' that will be used for drug testing in various areas of medicine.

# data[].attributes.body
Due to the risk of affecting fetuses, this technology cannot be tested on pregnant mothers. Donated placentas are being used but are only viable for a few hours. This could create difficulty in collecting accurate data.

# data[].attributes.body
This is a great step toward a breakthrough, as many pregnant women suffer through medical complications due to halting a medically necessary medication, that may cause risk to the fetus. I have personally gone through this with migraine medication while pregnant. As a nurse in psychiatry, I had pregnant patients who couldn't take their psychiatric drugs due to this issue, which imposed a different risk to the mother and fetus altogether.

# data[].attributes.body
As this technology advances and is implemented, nurses may use this to educate and integrate this into their evidence-based practice.
There may also be nurses involved in the research itself by collecting and analyzing data. They may also design and develop methods to integrate the technology into patient-focused care.

# data[].attributes.body
Bloomberg. (2015, July 29).

Drug testing shake up with organs-on-a-chip. Retrieved from https://www.youtube.com/watch?v=rI8piLXJawQ&t=3s

Brusie, Chaunie. (2015, August 19). Pregnancy: Category C drugs you should avoid. Retrieved from

https://www.healthline.com/health/pregnancy/category-c-drugs#

Lerner, Evan. (2016, July 21).

Penn researchers develop placenta-on-a-chip. Retrieved from https://news.upenn.edu/news/penn-researchers-develop-placenta-chip