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How does the temperature affect photosynthesis in leaf disks?

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Sodium Bicarbonate, ice, hot plate, light, hole puncher, 4 plastic syringes, leaves, timer, thermometer, 4 beakers

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If the temperature increases, the rate of photosynthesis will increase, until coagulation.

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Control, Factors Held Constant, Independent and Dependent Variables

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Control, Factors Held Constant, Independent and Dependent Variables

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Control

room temperature sodium bicarbonate solution

Factors Held Constant

-type of leaf

-type of light

-time kept in light

Independent Variable

time

Dependent Variable

amount of floating disks

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Cold Temperature

disks started floating later in the process

Room Temperature

the ideal temperature for the process, so worked the best

Warm Temperature

disks began to float earlier in the process

Hot Temperature

enzymes were coagulated, so no results were shown

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The data in our experiement shows that when the temperature increases from 10°C up to 65°C, the rate of photosynthesis increases until it gets too hot to the point of the coagulation of enzymes. On the graph, the slopes increase from 10°C up to 35°C, and the 65°C slope stays at 0. At minute 15, the 10°C data had a leaf float, and this is because at lower temperatures, the enzymes are not as effective, so the results are shown later in the process.

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1. Hole punch 40 disks out of a leaf

2. Put 10 in each syringe

3. Prepare a bicarbonate solution in beaker

4. Use thermometer to get the appropriate temperature in celsius, which is 10°C, 20°C, 35°C and 65°C-use hot plates and ice

5. Suck in half a syringe of bicarbonate solution

6. Create vacuum by covering tip to make the disks sink (10 seconds)

7. Place under light for 18 minutes

8. Count amount of floating disks per minute

9. Repeat for all temperatures