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We discuss some of the confusion among the team members. We do the report based on the slide presentation that we did before. We also plan to submit the report to Dr. Aisyah first before submit it in the Google form.

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Briefing session for Module 2 with Dr. Aisyah

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In this module 2 for Lab 4, our group had been assigned for the topic of Biosensor to detect Sulfonamides under the supervision of Dr. Aisyah. Hence, we have a briefing session with Dr. Aisyah to discuss on the labsheet for this lab. Dr. explain about the objectives, timeline and what we should do throughout this lab.

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Each one of us in Group 1 and 2 need to add our self introduction in the padlet given by Dr. Aisyah.

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After the briefing session, we have a separate meeting to discuss on the task given. I had been assigned to do the biosensor to detect antibiotics. We need to update all the information in the jamboard prepared by Dr. Aisyah.

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Week9_Update jamboard with biosensor information

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I need to handle objective 2. Since it is a bit difficult for me to understand and search all the mechanism for the biosensor stated, I decided to ask help from some of my teammates and distribute the task like this:
1. Magnetic nanoparticles (era)
2. Electrochemical biosensor (azra)
3. Fluorescent biosensor (farah)
4. Surface plasmon resonance (Sharifah)

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Week off (no class)

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Since this week we didn't have any class meeting, we should do the proposal and continue to search information on the sulfonamides detection using biosensor

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We update our progress during the meeting. We manage to finish updating all the slide in the Jamboard. The PIC for each objectives is as stated below:
Objective 1: Yuni
Objective 2: Sharifah
Objective 3: Farah and Era
Objective 4: Azra

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After the progress meeting, we have a meeting with our team members and we discuss on the type of biosensor. I briefly explain on the mechanism for each of the biosensor since I am the one who handle that part. We decided to choose the Electrochemical biosensor. We also discuss on the type of sulfonamides and we still confusing to choose which types of sulfonamides. So, we need to do more research on this.

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We finalized and do some discussion on the proposal that need to be submitted on the 2nd January.

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One of our team members, Azra had successfully submit the proposal in the elearning

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In the slack discussion, we put all the articles and idea that we found in their respective channel. In the design and material channel, we put our idea for the biosensor design and we do some discussion regarding each of the biosensor design proposed.

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Finally, we need to choose one design to be presented and mine was chosen but we only manage to present the 2D hand sketching drawing. Dr. want us to design it using the 3D software and I think Tinkercad is better since it is an online software that can be shared and design it together.

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After progress meeting update, we have a group meeting and the output is listed below:
1. Search

Mechanism of detection of smx (eg: oxidation process of Tyr when there are smx)

Material for casing which better PLA or thermoplastic resin

Percentages of smx concentration in water (safe level- dangerous level value)

What buffer need to be used and why

2. Biosensor design

Display (percentages of smx concentration in wastewater; level of severity; voltage use)

On the device have : button to increase voltage; led with multicolor; place to keep the electrode

Dimensions : 95 x 120 mm

LCD dimensions: 64 x 80 mm

Electrode using screen-printed carbon electrode that will be immobilized with Tyr by crosslinking it with GA

3. PIC slide: Azra, Era, Farah
PIC design: Yuni, Sharifah

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I design the biosensor using Tinkercad software based on the final sketching that been done by Yuni. Yuni and I discuss and do this design together in Tinkercad.

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We presented about our recent design for the biosensor and our progress on the slide presentation. Dr. had point out some comment regarding our design and Dr. suggest us to have a smaller electrode with simple and practical design. After having discussion on our progress, Dr. explain about how the tyrosinase-based biosensor mechanisms works.

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We have a group meeting and discuss about the improvement of our biosensor design. We split up the team into 3 team to have better understanding on each of the task. It is as listed below:
Design: Farah
Mechanism: Azra, Yuni
Percentage SMX: Era, Sharifah

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We have some discussion on slack on the materials that can be used for the biosensor. We shared the article in the slack group discussion because everyone can read the article as well.

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Finalized and completed the report before submit it for SV checking.

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I need to present from Introduction parts until Literature Review. Even though each one of us need to record less than 2 minutes, but I didn't manage to do so because there is a lot of information that I need to include. After minimalized the information and doing some edit in the video, I managed to get about 2 minutes and 40 seconds.

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All the team members need to record their parts separately using the Google Meet and upload it in the Google drive.

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Yuni had compile all the video and edit some parts. She upload it in the Youtube and submit the link in the Google form.

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This is our last meeting with Dr.Aisyah and we need to show our progress for the 3D design, presentation slides and report. Dr. gave a lot of comments to improve our slide presentation:
1. Title is too long
2. Problem Statement need to include objective and at the last sentences add the solution statement "therefore, this study been conducted......"
3. Objective: (only 2 objective)
3 -> 1
4 -> 2
4. Literature Review need to include citation
5. Mechanism need to be drawn in diagram

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Distribute task for slide presentation and video presentation

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We finalized the distribution task for the slide presentation and video presentation:
a. Title: Sharifah
b. Introduction/Background: Sharifah
- Sulfonamides; uses, types, effect
c. Problem statement: Sharifah
d. Objectives: Sharifah
e. Literature Review: Sharifah
- Biosensor, carbon, tyrosinase, sulfomethoxazone
f. Methodology:
- Materials; enzyme, Screen printed electrode, nanomaterials- Azra
- Fabrication process- Era
- Design consideration- Era
- Process design of the biosensor strip and body part- Farah
g. Result:
- final design (picture): Farah
- design summary (in table); name of product, function, type of biosensor and enzyme used, features, specification (dimension and material)- Azra
- mechanism on how biosensor work, output (include severity and calculation)- Azra
h. Discussion:
- Reason why choose that type of sulfonamide, biosensor and the enzyme, why choose that nanomaterial, why choose PLA for body Yuni
- State overall advantages of our biosensor and its limitation Yuni
i. Conclusion - Era
i. Selected References

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Week6_Divide task for video presentation (20hb)

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During the QnA session with the panelist, we had take note some of the important things that need to be improved especially in the report. It been listed here:
1. Problem Statement
- Health treat (specify)
- Limitation from early detection (SWNCT)
- Costly, time consuming (Other Biosensor compared to Electrochemical)
2. Methodology
- Fabrication process (This picture is adapted from..... )
- Design consideration (small size, simplicity of strip)
3. Discussion
- Why we choose TinkerCad
- Future research (nanoparticles that need to be used because of the drawback from MWNCTs, use Solidworks for simulation)

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Finalized and finish the slide before recording for the video presentation.

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Discussion on slide and video presentation

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We submit the report after editing some comments from our Sv, Dr. Aisyah.

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Formula for calculating the concentration of Sulfamethoxazole (SMX) and percentage of severity level for SMX