# data[].attributes.body
No conclusive findings because of the lack of comparability between control group and study group, the data collected did not show clear differences between amino acid levels in the two groups anyway.

# data[].attributes.body
The process of providing amino acid supplementation immediately after exercise resulted in increased resting levels of amino acids in plasma to support exercise and recovery.

# data[].attributes.body
The horses fitness level was not tested before the investigation, muscle biopsy and v02 max etc was not studied, therefore It would be unfair to say all horses were at the same fitness level before the investigation.

# data[].attributes.body
The horses that acted as the control group were not in work prior to the study which means they are not accurately comparable to horses that have been in high intensity training.

# data[].attributes.body
No information on whether a phase in period was included to gently adjust the horses digestive tracts to the new dietary supplement.

# data[].attributes.body
Sampling occurred regularly and routinely, so time of day, feed and exercise would not impact results as long as they were also regular and routine.

Samples were taken following high intensity exercise:
Good as this is when protein demands are highest so is relatable to when the issue matters most
But samples taken at a range of times and compared would give a more accurate overview of the effects of the supplement on overall health.

# data[].attributes.body
Study is looking heavily at variations in amino acid concentrations and whether this changes due to supplementation - the study states on two days each week supplementation was not possible due to routines of the horses - could possibly affect the spectrum of results recorded.
'With the exception of race day restrictions when it was not possible for the horses to receive the supplement, the horses were provided with a complex amino acid supplement daily for 34 days '

# data[].attributes.body
No records of any injuries which may have taken place during the investigation itself.

# data[].attributes.body
Standardbreds used - bred for performance but in comparison with a native breed results could differ dramatically.
However this would apply more to athletic performance horses than for example a show cob, thus improving relevance to industry.

# data[].attributes.headline
No preliminary investigation into whether feeding the supplementation 3 weeks prior to the study would have any effect on the horse 

# data[].attributes.subject
No preliminary investigation into whether feeding the supplementation 3 weeks prior to the study would have any effect on the horse 

# data[].attributes.body
Analysing amino acid concentrations in the horse - fluctuations and variations in hormones between sexes may occur, possibly altering the data set and forming the basis of a different conclusion.
Looking at both genders could of given contrast between the data groups
However due to the small sample size this could of produced confounding variables between the gender groups.

# data[].attributes.body
Aims-
The aim of the study was to look at the losses of amino acids in Standardbred horses and the application of supplementation strategies to maintain condition during training.

# data[].attributes.body
'Two cohorts of five and six standardbreds'
Does not give a reliable data set due to a smaller range of individuals - results may not be gene