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--sounds familiar? As it applies, the use of Wistar-Kyoto rats in research is wider than you thought. In our childhood, we are fancied with the French rat chef starring in Ratatouille(2007) --or the rat who happened to fall in the sewers ended up literally falling in love as portrayed in the movie Flushed Away(2006). The rats in these movies are outbred stock until they are inbred reaching more than 20th filial generation. To answer the OST Barbie in this case, I attached a Youtube link for a clearer explanation.

Disclaimer: Barbie has nothing to do with WKY rats. I'm just hyping up the official soundtrack of a great movie!

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What Was I Made For? [From The Motion Picture "Barbie"]

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What Was I Made For? [From The Motion Picture "Barbie"]

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What are the King-Phillip-Came-Over-For-Gooseberry-Soup of the animal we're discussing here? Let's find out!

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INBRED describes closely related animal mating. Animal physiological variations, such as internal, invisible changes in physical or chemical function, are referred to as STRAIN.

Together, it makes inbred strain almost homozygous at every locus.

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It varies depending on their origin and breeding history!

A simple guideline as follows:

strain symbol all written in capital letters and/or numbers

substrain designation after a forward slash "/"

substrain designation given as per registered code

followed by <space>derivation method

<space>hygienic state code written in all capital letters

<space>filial generation number in parentheses "()"

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Charles River Laboratories, 2024. Rats of different strains share similar morphology in form or function as they are of the same breed. They differ in physiology such as physical and/or chemical function which are internal and invisible.

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Body weight: 200-400g

Body length: ~25cm

Body temperature: 35.9-37.5°C

Common features: pointed snout, short hairs, round and erect ears, and a long, hairless, scaly tail. Albino colouring, low fertility and calm behavior.

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Male

longer anogenital distance (~2x than female)

scrotum and testicles should be visible between 3-4 weeks of age

Female

shorter anogenital distance

vaginal opening is visible

When sexing rats, it is best to wait at least 2-3 weeks old and hold 2 rats to compare the anogenital distance.

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AI prompt (perplexity.com): Research application of Wistar Kyoto rats

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AI prompt (perplexity.com): Research application of Wistar Kyoto rats

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as normotensive controls in studies on Spontaneously Hypertensive Rats(SHR) (Zahraa Mohammed-Ali et al., 2017)

as a model of depression-like symptoms (Wright et al., 2020). In this case, the scientists get to explore anhedonia -reduced ability to experience pleasure or interest in previously enjoyable routine.

When evaluating anhedonia, the subjects are given sweetened solutions, such as sucrose or saccharin. Hedonic reaction to pleasant sweet flavors is the responsive variable. However, consideration is given to additional small possible factors affecting the reaction. This has led to the development of more accurate hedonic behavior assays.

Other research applications include vascular dysfunction, pharmacological and toxicological studies.

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1971: WKY/Crl

1974: WKY/Tac

1982: WKY/Hrl

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AI prompt (perplexity.com): Development of Wistar Kyoto rats

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AI prompt (perplexity.com): Development of Wistar Kyoto rats

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debuted as normotensive(having normal blood pressure) strain

They were not fully inbred before being given to various breeders, which resulted in a notable degree of variation in the research's findings.

Their genetic and behavioral divergence being influenced by breeding and dispersal methods

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Nomenclature:

WKY/Crl

WKY/Tac

WKY/Hrl

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The best video I could find on Youtube even though I had to tilt my head for a while.. hope it helps!

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The most convenient AI prompt so far because it provided the links for further readings and aids in finding reliable sources.

Other than that, I use MyBib as citation generator.

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Example: WKY/KyoRj f SPF (F78)

strain symbol: WKY denotes Wistar-Kyoto strain

common breeder/origin: KyoRj denotes CSAL(Orléans, from Kyoto University)

derivation technique: f denotes fostered

hygienic state: SPF denotes specific pathogen free

filial generation number: (F78) indicates the rat are at the 78th filial generation of breeding