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Explanation of how temperature, pH and substrate concentration affect enzyme activity. You need to be able to SKETCH the graphs resulting from changing each of these independent variables on the rate of enzyme activity as well as be able to EXPLAIN the effect of each variable on enzyme activity.

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3.6.3 Explain factors affecting enzyme activity

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Chemistry for Biologists resources aim to help you understand the chemistry and chemical principles that underlie a good deal of biology. These resources were hosted on the Chemistry for Biologists website, which launched in 2004 and was supported by the Royal Society of Chemistry and the Biochemical Society. From 2019 Chemistry for Biologists resources are hosted on the Royal Society of Biology website.

The resources are aimed at post-16 students taking biology (or related subjects) to A level, Scottish Higher or similar level. These will also be of use to first year undergraduates studying biology. The resources assume you have studied some chemistry (either a separate subject or as part of a balanced science course to GCSE level or equivalent). The material is organised into 17 topics, which can be approached in any order, although it might be a good idea to tackle Some basic chemistry first. Each chapter has a short multiple choice test accompanying it that you can take at any time. You can use these tests in a number of ways:

1. Before tackling each chapter to help you decide whether or not you need to work through the chapter or not.

2. After studying a chapter to check your understanding; or

3. Before and after studying a chapter to see how much you have improved

Most chapters include:

Text

Diagrams

Links to other websites

The resources originally presented 3-D rotatable structures, these are no longer supported, but we suggested that you explore http://molview.org/ instead. Just be wary of the many different isomers you will find, make sure you follow the biologically relevant examples. The rotatable molecules are important because a great deal of biochemistry depends on the three-dimensional shapes of molecules - how substrates fit the active sites of enzymes, how drug molecules fit receptors in cells, for example.

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About Chemistry for Biologists Chemistry for Biologists resources aim to help you understand the chemistry and chemical principles that underlie a good deal of biology. These resources were hosted on the Chemistry for Biologists website, which launched in 2004 and was supported by the Royal Society of Chemistry and the Biochemical Society.

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The activity of an Enzyme is affected by its environmental conditions. Changing these alter the rate of reaction caused by the enzyme. In nature, organisms adjust the conditions of their enzymes to produce an Optimum rate of reaction, where necessary, or they may have enzymes which are adapted to function well in extreme conditions where they live.

Temperature

Increasing temperature increases the Kinetic Energy that molecules possess. In a fluid, this means that there are more random collisions between molecules per unit time.

Since enzymes catalyse reactions by randomly colliding with Substrate molecules, increasing temperature increases the rate of reaction, forming more product.

However, increasing temperature also increases the Vibrational Energy that molecules have, specifically in this case enzyme molecules, which puts strain on the bonds that hold them together.

As temperature increases, more bonds, especially the weaker Hydrogen and Ionic bonds, will break as a result of this strain. Breaking bonds within the enzyme will cause the Active Site to change shape.

Graph showing a typical variation of enzyme activity with temperature

The temperature at which the maximum rate of reaction occurs is called the enzyme's Optimum Temperature. This is different for different enzymes. Most enzymes in the human body have an Optimum Temperature of around 37.0 °C.

pH - Acidity and Basicity

pH measures the Acidity and Basicity of a solution. It is a measure of the Hydrogen Ion () concentration, and therefore a good indicator of the Hydroxide Ion () concentration. It ranges from pH1 to pH14. Lower pH values mean concentrations and concentrations.

Acid solutions have pH values below 7, and Basic solutions (alkalis are bases) have pH values above 7. Deionised water is pH7, which is termed ' neutral '.

H⁺ and OH⁻ Ions are charged and therefore interfere with Hydrogen and Ionic bonds that hold together an enzyme, since they will be attracted or repelled by the charges created by the bonds. This interference causes a change in shape of the enzyme, and importantly, its Active Site.

Different enzymes have different Optimum pH values. This is the pH value at which the bonds within them are influenced by H⁺ and OH⁻ Ions in such a way that the shape of their Active Site is the most Complementary to the shape of their Substrate. At the Optimum pH, the rate of reaction is at an optimum.

Any change in pH above or below the Optimum will quickly cause a decrease in the rate of reaction, since more of the enzyme molecules will have Active Sites whose shapes are not (or at least are less) Complementary to the shape of their Substrate.

Graph showing a typical variation of enzyme activity with acidity

Small changes in pH above or below the Optimum do not cause a permanent change to the enzyme, since the bonds can be reformed. However, extreme changes in pH can cause enzymes to Denature and permanently lose their function.

Enzymes in different locations have different Optimum pH values since their environmental conditions may be different. For example, the enzyme Pepsin functions best at around pH2 and is found in the stomach, which contains Hydrochloric Acid (pH2).

Concentration

Changing the concentration of a substance only affects the rate of reaction if it is the limiting factor: that is, it the factor that is stopping a reaction from preceding at a higher rate.

If it is the limiting factor, increasing concentration will increase the rate of reaction up to a point, after which any increase will not affect the rate of reaction. This is because it will no longer be the limiting factor and another factor will be limiting the maximum rate of reaction.

As a reaction proceeds, the rate of reaction will decrease, since the Substrate will get used up. The highest rate of reaction, known as the Initial Reaction Rate is the maximum reaction rate for an enzyme in an experimental situation.

Substrate Concentration

Graph representing the change in reaction rate as substrate concentration increases

Enzyme Concentration

Increasing Enzyme Concentration will increase the rate of reaction, as more enzymes will be colliding with substrate molecules.

However, this too will only have an effect up to a certain concentration, where the Enzyme Concentration is no longer the limiting factor.

Graph representing the change in reaction rate as enzyme concentration increases

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The activity of an Enzyme is affected by its environmental conditions. Changing these alter the rate of reaction caused by the enzyme. In nature, organisms adjust the conditions of their enzymes to produce an Optimum rate of reaction, where necessary, or they may have enzymes which are adapted to function well in extreme conditions where they live.