# data[].attributes.body
BY: Tyler Campbell

# data[].attributes.body
The body uses enzymes to speed up chemical reaction within the human body. The body creates peroxide, which is a toxic chemical. Catalase break down the peroxide in the liver to turn it into water and oxygen. During this lab we will witness how the liver reacts with the peroxide.

# data[].attributes.body
The purpose of this lab is the prove that the enzyme(catalase) in the liver will break down peroxide and produce water and oxygen.

# data[].attributes.body
·       Water

·       Peroxide

·       (5) testing tubes

·       (5) pieces of liver

·       (5) wooden splints

·       Box of matches

·       Test tube holder

# data[].attributes.body
Step 1: Pour water in the first test tube up to the 2cm mark.

Step 2: Place a piece of liver in the water of the first test tube by pushing it down the wooden splint.

Step 3: Pour peroxide in the 2nd tube up to the 2cm mark.

Step 4: Place a piece of liver into the peroxide of the 2nd tube. Use wooden splint if necessary.

Step 5: Add peroxide in the 3rd tube up to the 2cm mark.

Step 6: Light the wooden splint and let it burn until the flame becomes nearly gone.

Step 7: While the flame is burning red, put it into the peroxide.

Step 8: Add 2cm of peroxide into the 4th tube.

Step 9: Add a piece of liver into the peroxide of the 4th tube.

Step 10: Light the wooden splint.

Step 11: As the flames become red, quickly strike the bubbles multiple times with the wooden splint. Sparks should occur.

Step 12: Add water and a piece of liver to the 5th test tube. Let it boil.

Step 13: Place peroxide into the 5th test tube.

Step 14: Record the reaction for each test tube.

# data[].attributes.body
Enzymes play a very important role in the body, a role that most would not notice. The liver is an enzyme that creates catalase that breaks down toxic peroxide into water and oxygen. The lab shows how the peroxide and liver react to make water and oxygen. This experiment proves that the liver preforms this much important role.

# data[].attributes.body
What are enzymes?  What are their roles in chemical reactions?

Enzymes are proteins in the body that speed up the rate of chemical reaction, by decreasing the amount of energy needed. Enzymes grab on to specific fitting substrates and create new products.

Describe what happened to test tube 2 when hydrogen peroxide was added to the liver.  What gas was produced?  What liquid was left behind?

Hydrogen peroxide was added to the liver, the enzyme(liver) produced oxygen from this reaction and left the water behind. The water and oxygen products from the chemical reaction between the liver and peroxide.

What effect does the red hot wood have on test tube 4? WHY is the flame being produced?

Oxygen is a gas that is flammable. When the wooden splint sparks when it touches tube 4, this is proof that when the liver and the peroxide mix that oxygen is the product of the chemical reaction. Since oxygen is flammable, the contact from the wood makes the spark.

What effect does temperature have on the enzymes (Test tube 5)?

Test tube #5 represents when an enzyme becomes too hot and above optimal temperature. The enzyme denatures and changes shape, and as we know the shape is critical for the chemical reaction. The change of shape causes the substrate(peroxide) not to react with the enzyme.