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~710 BC – The Nimrud lens – a piece of rock crystal – may have been used as a magnifying glass or as a burning-glass to start fires by concentrating sunlight.

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~710 BC – The Nimrud lens – a piece of rock crystal – may have been used as a magnifying glass or as a burning-glass to start fires by concentrating sunlight.

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In 1874 – Abbe equation

Ernst Abbe writes a mathematical formula that correlates resolving power to the wavelength of light. Abbe’s formula makes it possible to calculate the theoretical maximum resolution of a microscope.
Example of Abbe equation

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In 1931 – Transmission electron microscope

Ernst Ruska and Max Knoll design and build the first transmission electron microscope (TEM), based on an idea of Leo Szilard. The electron microscope depends on electrons, not light, to view an object. Modern TEMs can visualise objects as small as the diameter of an atom.

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1932 – Phase contrast microscope

Frits Zernike develops phase contrast illumination, which allows the imaging of transparent samples. By using interference rather than absorption of light, transparent samples, such as cells, can be imaged without having to use staining techniques.

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1942 – Scanning electron microscope

Ernst Ruska builds the first scanning electron microscope, which transmits a beam of electrons across the surface of a specimen.

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~1021 AD – Book of Optics Muslim scholar Ibn Al-Haytham writes his Book of Optics. It eventually transforms how light and vision are understood.

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~1021 AD – Book of Optics Muslim scholar Ibn Al-Haytham writes his Book of Optics. It eventually transforms how light and vision are understood.

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1284 – First eye glasses Salvino D’Armate is credited with inventing the first wearable eye glasses.

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1284 – First eye glasses Salvino D’Armate is credited with inventing the first wearable eye glasses.

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1590 – Early microscope Zacharias Janssen and his son Hans place multiple lenses in a tube. They observe that viewed objects in front of the tube appear greatly enlarged. This is a forerunner of the compound microscope and the telescope.

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1590 – Early microscope Zacharias Janssen and his son Hans place multiple lenses in a tube. They observe that viewed objects in front of the tube appear greatly enlarged. This is a forerunner of the compound microscope and the telescope.

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1957 – Confocal imaging principle

Marvin Minsky patents the principle of confocal imaging. Using a scanning point of light, confocal microscopy gives slightly higher resolution than conventional light microscopy and makes it easier to view ‘virtual slices’ through a thick specimen.

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1962 – Green fluorescent protein (GFP) discovered

Osamu Shimomura, Frank Johnson and Yo Saiga discover green fluorescent protein (GFP) in the jellyfish Aequorea victoria. GFP fluoresces bright green when exposed to blue light.

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1972 – First CAT scanner

Godfrey Hounsfield and Allan Cormack develop the computerised axial tomography (CAT) scanner. With the help of a computer, the device combines many X-ray images to generate cross-sectional views as well as three-dimensional images of internal organs and structures.

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1973 – Electron backscatter patterns observed

John Venables and CJ Harland observe electron backscatter patterns (EBSP) in the scanning electron microscope. EBSP provide quantitative microstructural information about the crystallographic nature of metals, minerals, semiconductors and ceramics.

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1978 – Confocal laser scanning microscope

Thomas and Christoph Cremer develop the first practical confocal laser scanning microscope, which scans an object using a focused laser beam.

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1981 - Scanning tunnelling microscope

Gerd Binnig and Heinrich Rohrer invent the scanning tunnelling microscope (STM). The STM ‘sees’ by measuring interactions between atoms, rather than by using light or electrons. It can visualise individual atoms within materials.

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1986 – Nobel Prize for microscopy

The Nobel Prize in Physics is awarded jointly to Ernst Ruska (for his work on the electron microscope) and to Gerd Binnig and Heinrich Rohrer (for the scanning tunnelling microscope).

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1992 – Green fluorescent protein (GFP) cloned

Douglas Prasher reports the cloning of GFP. This opens the way to widespread use of GFP and its derivatives as labels for fluorescence microscopy (particularly confocal laser scanning fluorescence microscopy).

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1993–1996 – Super-resolution microscopy

Stefan Hell pioneers a new optical microscope technology that allows the capture of images with a higher resolution than was previously thought possible. This results in a wide array of high-resolution optical methodologies, collectively termed super-resolution microscopy.

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Robert Hooke, experiments curator at the Royal Society of London, is generally acknowledged as being the first to observe cells. In about 1660, he improved the existing single-lens microscope design by placing a second lens in the viewing tube. When appropriately placed relative to the other components, the second lens allowed for much greater magnification of a sample; he created the first compound microscope. Modern compound microscopes can magnify a specimen 1000 to 2000 times.

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2010 – Atoms of a virus seen

Researchers at UCLA use a cryoelectron microscope to see the atoms of a virus.

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2014 – Chemistry Nobel prize for super microscopes

Nobel Prize in Chemistry awarded to Eric Betzig, Stefan Hell and William Moerner for the development of super-resolved fluorescence microscopy which allows microscopes to now ‘see’ matter smaller than 0.2 micrometres.

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In 1830 Spherical aberration solved

Joseph Jackson Lister reduces spherical aberration (which produces imperfect images) by using several weak lenses together at certain distances to give good magnification without blurring the image.