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1)3
2) Wrong type of amylase. Would expect optimum to be 7 but was 3.
3) Could have had more syringes

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1) ph 3 as the time taken for the amylase to digest was the shortest.
2) It seems strange as Amylase is usually found in the mouth and ph 3 is very acidic. You would think that it would be more neutral/alkaline.
3) Using the same iodine for each test and by human error when you are deciding that there is no longer a colour change in the iodine when the solution is dropped in.

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Ph 3
Ph in mouth is different - different type of amylase
Use more syringes

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Our optimum pH was at 3. Because it was the shortest amount of time it took to break down all the starch into its monosaccharides. This is because we would think that our body’s average pH is 7, this is most probably because it is produced in the mouth, in our mouth there is nothing very acidic so it should be neutral, but because it goes into our stomach through our oesophagus. It has to be alkaline so it can’t hurt us and creates the neutralisation reaction. If we want to improve this experiment, we should first increase the possible amount of time we can measure. With only one spotting tile,  we can only measure 4 minutes at maximum, some of these reactions took longer than 4 minutes to complete.

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1. Optimum pH: pH 3
2. Was strange as amylase is in the mouth which would suggest the optimum may be around 7.
3. Improvement: In order to measure the curve of rate of reaction, we could use a colorimeter to identify light absorbance/color intensity.

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1. What was the optimum pH for amylase and why?

2. Why might the optimum pH in this experiment seem strange and can you think of an explanation?
3. One possible error and improvement for the method?

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Optimum PH: pH3
I think it is strange the humans amylase optimum PH is 7 but I believe this is from a different animal there for has a different Ph in the mouth.
We may of  mixed up the syrintes at some point

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1) pH3, because it has the right amount of H+ ions
2) The optimum pH for the amylase in your mouth is pH7, but the reason the pH for the amylase we used is different is because it is a different amylase, therefore it has a different pH.
3) We could use a colorimeter to more accurately determine the rate of reaction.

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1) The optimum pH for amylase in this experiment is 3. It takes the shortest time to break down starch (turn the iodine to brown).
2) The optimum pH (3) seems strange because usually the amylase in the mouth (used to break down foods) is 7. The amylase in our mouth is neutral (7) and Ph3 is very acidic.
3) Making sure that the iodine drops the are placed on time.

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optimum pH: 3
Because it turned brown basically instantly. It was acidic to help break down the starch.
2: It is strange because we thought we thought that 7, the neutral ph, would be most effective. And our saliva has a pH of 7.
3: Mixing the pipettes up.

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Optimum pH: 3
It took the least amount of time to break down the starch and turn brown.
It was strange because we thought since it was in the mouth it would be pH 7.
To improve we could use a colorimeter so that we can improve the accuracy of the data.

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optimum pH: 3
Because it turned brown basically instantly. It was acidic to help break down the starch.
2: It is strange because we thought we thought that 7, the neutral ph, would be most effective. And our saliva has a pH of 7.
3: Mixing the pipettes up.

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1. The optimum pH for amylase is 3, The acidity helps break down the starch
2. It was strange because we got an optimum pH of 7.
3. Use a colorimeter to measure the colour absorption and intensity.

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1. 3
2. It was strange because we thought that the neutral pH 7 in your mouth would be the most effective
3. Using the wrong syringes and forgetting to time the right pH.

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1. 3
2. you would think that the acidity in the ph would denature the enzymes in the amylase
3. be more precise

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1) 3
2) It seems strange because amylase is usually found in the mouth. And a ph of 3 is quite acidic
3) Could have more syringes

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1) The optimum PH is 3 as the rate of reaction is fastest at this PH

2) Possibly because the amalyse is found in the mouth and the ph is 7

3) more spots in the plate so we can measure more time and more accurately as time increments are quite large

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1. 3, because at PH3 none of the spots changed to brown/ black
2. Yes, expected to be 7
3. Collect data carefully

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1. 3
2. We were expecting 7 to be the optimum as the pH in the mouth is generally neutral
3. Accuracy whilst pouring, it would be better if we used some measuring cylinders instead.

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1. Optimum pH was 3
2. It is strange because you'd think the pH should be neutral, as saliva is supposed be neutral, so you'd think it would be 7 pH.
3. Making sure you use a stopwatch that sounds every 20 seconds maybe, as we would be delayed a few seconds sometimes because we didn't realize.

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1) 3
2) Optimum PH in mouth is 7. Different type of amylase?
3) Might have been different amounts of Iodine in each drop.

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1. The optimum pH was 3.
2. This is quite surprising as the pH of the mouth where amylase breaks down starch is around 7. Why is this?
3. One error would be making sure to extract the amylase solution from the test tube on time. Also, making sure that all iodine drops were of equal amount. And improvement might be to test more pH levels between the one we did in order to get an image of the bigger picture.