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Staining - Chemicals bind to parts of the cell to create contrast so the cell can be seen Waxing - sample is embedded in wax so that thin strips can be cut without distortion

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+ Higher resolution can be achieved + Detail of internal structure

- High level of skill to prepare samples an use equipment - Equipment is expensive - Samples need to be placed in a vaccum as electrpns can be deflected by the air

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Max Resolution - 0.05nm Max Magnification - x500,000

- 2D - Passes through sample

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Max Resolution - 20nm Max Magnification - x100,000

- 3D - Bounce off sample

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Max R esolution - 0.2 um Max Magnification - x1500

+ Wide range of specimens can be viewed - Only thin specimens can be used - Internal detail cannot be seen

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The ability to distinguish between two points separately

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How many times greater an image is than the original object