# data[].attributes.body
| The main aim of this study was to evaluate the impact of human energy treatment on 2,6-Diclorophenol and 2,4-Dichlorophenol, using GC-MS analysis technique.

# data[].attributes.headline
Evaluation of Biofield on Dichlorophenol Isomers

# data[].attributes.subject
Evaluation of Biofield on Dichlorophenol Isomers

# data[].attributes.body
Dichlorophenol isomers, were studied with GC-MS analysis and a significant change in treated isotope abundance of 2H/1H or 13C/12C, and 18O/16O or 37Cl/35Cl was observed.

# data[].attributes.attachment_link.title
Determination of Isotopic Abundance of 2H, 13C, 18O, and 37Cl in Biofield Energy Treated Dichlorophenol Isomers

# data[].attributes.attachment_link.description
2,4-Dichlorophenol (2,4-DCP) and 2,6-dichlorophenol (2,6-DCP) are two isomers of dichlorophenols, have been used as preservative agents for wood, paints, vegetable fibers and as intermediates in the production of pharmaceuticals and dyes. The aim of the study was to evaluate the impact of biofield energy treatment on the isotopic abundance ratios of 2H/1H or 13C/12C, and 18O/16O or 37Cl/35Cl, in dichlorophenol isomers using gas chromatography-mass spectrometry (GC-MS).

# data[].attributes.headline
Energy Healing Treatment | Dichlorophenol Isomers

# data[].attributes.subject
Energy Healing Treatment | Dichlorophenol Isomers

# data[].attributes.body
The objective of the present research was to assess the effect of energy healing treatment on the isotopic abundance ration in dichlorophenols. Follow the link for further details.

# data[].attributes.body
| The current study shows the influence of unique alternative energy treatment on the isotopic abundance ratio in 2,4-Dichlorophenol. Read more details by following the provided link.

# data[].attributes.attachment_link.title
Determination of Isotopic Abundance of 2H, 13C, 18O, and 37Cl in Biofield Energy Treated Dichlorophenol Isomers | Mahendra Trivedi | Alice Branton | Dahryn Trivedi | Gopal Nayak | Snehasis Jana | Academic Room

# data[].attributes.attachment_link.description
2,4-Dichlorophenol (2,4-DCP) and 2,6-dichlorophenol (2,6-DCP) are two isomers of dichlorophenols, have been used as preservative agents for wood, paints, vegetable fibers and as intermediates in the production of pharmaceuticals and dyes. The aim of the study was to evaluate the impact of biofield energy treatment on the isotopic abundance ratios of 2H/1H or 13C/12C, and 18O/16O or 37Cl/35Cl, in dichlorophenol isomers using gas chromatography-mass spectrometry (GC-MS).

# data[].attributes.body
| Read here about the impact of biofield energy treatment on the isotopic abundance ratios of 2H/1H or 13C/12C, and 18O/16O or 37Cl/35Cl in 2,6-DCP using  the GC-MS analysis.

# data[].attributes.attachment_link.readable_text
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Science Journal of Analytical Chemistry

Published online December 21, 2015 (http://www.sciencepublishinggroup.com/j/sjac)

doi: 10.11648/j.sjac.20160401.11

ISSN: 2376-8045 (Print); ISSN: 2376-8053 (Online)

37Cl in Biofield Energy Treated Dichlorophenol Isomers

Trivedi Global Inc., Henderson, USA

Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Gunin Saikia, Snehasis Jana. Determination of Isotopic Abundance

Cl in Biofield Energy Treated Dichlorophenol Isomers. Science Journal of Analytical Chemistry.

Vol. 4, No. 1, 2016, pp. 1-6. doi: 10.11648/j.sjac.20160401.11

2,4-Dichlorophenol (2,4-DCP) and 2,6-dichlorophenol (2,6-DCP) are two isomers of dic hlorophenols, have been

used as preservative agents for wood, paints, vegetable fibers and as intermediates in the production of pharmaceuticals and

dyes. The aim of the study was to evaluate the impact of biofield energy treat ment on the isotopic abundance ratios of

Cl, in dichlorophenol isomers using gas chromatography-mass spectrometry (GC-MS). The 2,4-

DCP and 2,6-DCP samples were divided into two parts: control and treated. T he control sample remained as untreated, while

the treated sample was further divided into four groups as T1, T2, T3, and T4. The treated group was subjected to Mr. Trivedi's

biofield energy treatment. The GC-MS spectra of 2,4-DCP and 2 ,6-DCP showed three to six m/z peaks at 1 62, 126, 98, 73, 63,

37 etc. due to the molecular ion peak and fragmented peaks. The isotopic abundance ratios (percentage) in both the isomers

were increased significantly after biofield treatment as compared to the control. The isotopic abundance ratio of (PM+1)/PM

and (PM+2)/PM after biofield energy treatment were increased by 54.38% and 40.57% in 2,4-DCP and 126.11% and 18.65%

in 2,6-DCP, respectively which may affect the bond energy, reactivity and finally stability to the product.

Biofield Energy Treatment, 2,4-Dichlorophenol, 2,6-Dichlorophenol, Gas Chromatography-Mass Spectrometry

pesticides or converted into pesticides by chemical means.

2,4-DCP and 2,6-DCP are two isomers of dichlorophenols,

and they have been used as preservati ve agents for wood [1,

addition, they are used as herbicides, fungicides insectic ides

and as inter mediates in the pro duction of p harmaceuticals

chlorination of phenol using chlorine gas [5]. In the technical

product, there are impurities of other chlorophenol isomers or

chlorophenols with more or less chlorine [6]. Environmental

contamination is mainly occurred during manufacturing,

storage, transportation and application of chlorophenols. In

recent years, public health authorities are concerned about

the air borne contaminations and so germicidal paints has

been proposed in reducing at mospheric microbial pollutions

[7]. Keeping in mind of the applicability in large quantities

the stability of these isomers is most desired quality that

determines the life o f the finished product. Hence, it is

important to find out an alternate approach that could

enhance the chemical stability of the compound by altering

the structural pr operties. Recently, biofield energy treatment

is reported to alter the physicochemical p roperties and even

isotopic ratios of various elements significantly in a molecule

isotopic abundance ratios of

O, could be locally altered by kinetical ly driven

chemical reactions. There is an alternative and well-known

known as The Trivedi Effect

dichlorophenol isomers to undergo the isotopic changes.

The distribution of co ntaminant sources of any molecule

on a native or global scale can be understood by deter mining

the isotopic abundance ratio [11]. Any kinetic process that

leads to the local depletion or enhance ment of isotopes in

organic molecules can be successfully determined using gas

2 Mahendra Kumar Trivedi et al.: Determination of Isotopic Abundance of

Biofield Energy Treated Dichlorophenol Isomers

chromatography-mass spectrometry (GC-MS) [12]. These

deviations from perfect chemical equivalence are termed as

isotope effects. The isotopic abundance ratio is co mmonly

reported in terms of atom percent and determined by hi gh

resolution mass spectrometry (HRMS sp ectrometry) [13].

Moreover, the rate of chemical reaction may vary with t he

mass of the nucleus with d ifferent isotopic substitutions,

which slightl y affect the partitioning of energy within the

molecules [14]. Study the impact of biofield energy treatment

on isotopic abundance ratio of elements in a molecule is new

to its kind and our group have successfully designed and

The National Center for Complementar y and Alternative

Medicine (NCCAM) has recommended the use of energy

therapy as a part of Complementary and alternative medical

therapies (CAM) in the healthcare sector [15]. CAM include s

numerous energy-healing therapies, in which the biofield

therapy is a form of putative energy therap y that is being

widely used worldwide to improve the overall health o f

human beings. Humans have the ability to harness energy

from the environment/universe that can be transmitted to any

objects around. The ability of Mr. Trivedi as a biofield energy

physicochemical properties of various molecules and crystals

were altered by utilizing Mr. Trivedi's biofield energy

treatment [16-18]. Based on the previous results achieved by

The Trivedi Effect in various fields, biofield energy treated

2,4-DCP and 2,6-DCP were taken for mass spectroscopy

studies to evaluate the isotopic abundance ratio of

[where PM is the primary molecule and (PM+1) and (PM+2)

(2,6-DCP) were procured from Lobachemie Pvt. Ltd., India

and S. D. Fine Chemicals Pvt. Limited, India, respectively.

Each of the 2,4-DCP a nd 2,6-DCP samples was divided

into t wo parts, one part remained untreated and called as

control and the other part was subjected to Mr. Trivedi's

conditions, that considered as treated sample. The treated

sample was further divided into four groups (i.e. T1, T2, T3,

and T4) for GCMS analysis. There are no differences a mong

the treated samples T1, T2, T3, and T4, except time. The

isotopic abundance ratio of [(PM+1)/PM] and [(PM+2)/PM]

in control and treated samples were characterized using gas

chromatography-mass spectrometry (GC-MS).

GC-MS analysi s was done on Perkin Elmer/auto system

XL with Turbo mass, USA. The GC/MS was pe rformed in a

silica capill ary column. It was equipped with a quadrupole

detector with pre-filter, one of the fastest, widest mass

ranges available for any GC/MS. T he mass spectrometer

positive/negative, and chemi cal ioni zation mo de at the

electron ionization energy of 70 eV. Mass range: 20-620

Daltons (amu) , stabili ty: ± 0.1 m/z mass accuracy over 48

hours. The identi fication of anal ytes were done by retention

time and by a co mparison of the mass spectra o f identified

substances with refere nces.

The isotopic abundance ratio (PM+1)/PM and (P M+2)/PM

compared to the control. The percentage change i n isotopic

ratio was calculated from the following formula:

Percent change in isotopic abundance ratio

a re the r atios of i ntensity at

(PM+1) or (PM+2) to PM in mass spectra of treated and

control samples, respectively.

3. Results and Discussion

3.1. GC-MS Study of 2,4-Dichlorophenol

The mass spectrum obtained for the control sample of 2,4-

DCP in the positive-ion mode is illustrated in Fig. 1. It gives

molecular ion peak at m/z 162 with two major fragmented

peaks in lower m/z region that are well matched with the

literature report [19]. The peaks at m/z 64 a nd 98 were due to

the fragme ntation of 2,4-DC P to C

respectively. The GC-MS spectra of treated samples were

control sample. The GC-MS spectra of treated (T1, T2, T3,

and T 4) samples are presented in Fig. 2. It showed three

major peaks including molecular ion peak in T1 sample of

2,4-DCP with the base peak at m/z 63 (C

cases (control and treated) mass spectra showed the loss of

ions in the fragmentation process, followed by the

ring opening reaction, corresponded to the following ions,

, m/z 98 and 63. Most unlikely,

there were four major peaks observed in T2, with an

additional peak at m/z 73 (C

). However, similar to the

control sample, three major peaks at m/z 162, 98 and 63,

were observed in T3 and T4.

abundance ratio of all three elements are presented in Table

(PM+2)/PM in the control and treated 2,4-DCP was plotted

Science Journal of Analytical Chemistry 2016; 4(1): 1-6 3

Figure 1. GC-MS spectrum of control 2,4-dichlorophenol.

Table 1. GC-MS isotopic abundance analysis result of 2,4-dichlorophenol.

It was clearly seen from the Fig. 3 tha t, the isotopic

increased by 54.38% and 40.57%, respectively in treate d

samples of 2,4-DCP as compared to t he control. The

(PM+1)/PM and (PM+2)/PM in 2,4-DCP may have high

impact on the bond energies and reactivity o f the molecules

after biofield energy treatment. The increased isotopic

treated samples may i ncrease µ (effective mass) and binding

energy in the 2,4-DCP molecule with heavier isotopes, and

this may result in enhancing binding energy and stability of

Figure 2. GC-MS spectra of treated 2,4-dichlorophenol samples (T1, T2, T3, and T4).

# data[].attributes.attachment_link.authors[].name
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Gunin Saikia, Snehasis Jana

# data[].attributes.attachment_link.title
Determination of Isotopic Abundance of 2 H, 13 C, 18 O, and 37 Cl in Biofield Energy Treated Dichlorophenol Isomers - PdfSR.com

# data[].attributes.attachment_link.description
2,4-Dichlorophenol (2,4-DCP) and 2,6-dichlorophenol (2,6-DCP) are two isomers of dichlorophenols, have been used as preservative agents for wood, paints, vegetable fibers and as intermediates in the production of pharmaceuticals and dyes.

# data[].attributes.headline
Reactivity & Stability Factor of Dichlorophenol Isomers

# data[].attributes.subject
Reactivity & Stability Factor of Dichlorophenol Isomers

# data[].attributes.body
In the research, the isotopic abundance ratio of treated sample increased significantly, which may affect the bond energy, reactivity and finally stability to the product. To know more, follow the provided link.

# data[].attributes.attachment_link.keywords[]
isotopic abundance in dichlorophenol isomers

# data[].attributes.attachment_link.keywords[]
intermediates in the production of pharmaceuticals

# data[].attributes.attachment_link.keywords[]
dichlorophenol isomers used as herbicides

# data[].attributes.attachment_link.title
"Determination of Isotopic Abundance of 2H, 13C, 18O, and 37Cl in Biofield Energy Treated Dichlorophenol Isomers" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
2,4-Dichlorophenol (2,4-DCP) and 2,6-dichlorophenol (2,6-DCP) are two isomers of dichlorophenols, have been used as preservative agents for wood, paints, vegetable fibers and as intermediates in the production of pharmaceuticals and dyes. The aim of the study was to evaluate the impact of biofield energy treatment on the isotopic abundance ratios of 2H/1H or 13C/12C, and 18O/16O or 37Cl/35Cl, in dichlorophenol isomers using gas chromatography-mass spectrometry (GC-MS).

# data[].attributes.headline
Study of Isotopic Abundance: Dichlorophenol Isomers

# data[].attributes.subject
Study of Isotopic Abundance: Dichlorophenol Isomers

# data[].attributes.body
Due to the influence of unique human energy treatment, the isotopic abundance ratios in Dichlorophenol isomers i.e. 2,4-DCP & 2,6-DCP were altered significantly as compared to the control.

# data[].attributes.body
The GC-MS analysis of 2,4-Dichlorophenol shows the impact of unique non touch human energy treatment on the isotopic abundance ratio of H/1H or 13C/12C, and 18O/16O or 37Cl/35Cl.

# data[].attributes.attachment_link.title
Determination of Isotopic Abundance of 2H, 13C, 18O, and 37Cl in Biofield Energy Treated Dichlorophenol Isomers

# data[].attributes.attachment_link.description
2,4-Dichlorophenol (2,4-DCP) and 2,6-dichlorophenol (2,6-DCP) are two isomers of dichlorophenols, have been used as preservative agents for wood, paints, vegetable fibers and as intermediates in the production of pharmaceuticals and dyes. The aim of

# data[].attributes.headline
Alteration in 2,6-DCP Isotopic Abundance

# data[].attributes.subject
Alteration in 2,6-DCP Isotopic Abundance

# data[].attributes.body
After the biofield treatment, the result suggest that there were some alterations observed in 2,6-dichlorophenol isotopic abundance ratio. Get to know more, from the link given below.

# data[].attributes.body
In the present research, the GC-MS analysis of 2,4-DCP showed the result in increase in isotopic abundance ratio of (PM+1)/PM and (PM+2)/PM in the biofield energy treated sample as compare to control.

# data[].attributes.headline
Biofield Treatment on Dichlorophenol Isomers

# data[].attributes.subject
Biofield Treatment on Dichlorophenol Isomers

# data[].attributes.body
The aim of this study was to evaluate the impact of biofield energy treatment on the isotopic abundance ratios in Dichlorophenol isomers. Visit the link for more details.

# data[].attributes.attachment_link.description
2,4-Dichlorophenol (2,4-DCP) and 2,6-dichlorophenol (2,6-DCP) are two isomers of dichlorophenols, have been used as preservative agents for wood, paints, vegetable fibers and as intermediates in the production of pharmaceuticals and dyes.