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Hi everyone

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The sustainability of microbial bioplastics, production and applications

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The sustainability of microbial bioplastics, production and applications

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Aranza Mondragón Cruz - A01769664

Ricardo Soltelo Alfaro - A01770107

Alondra Alcántara Sánchez - A01770047

Carlos David Vázquez Aguilar - A01769969

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Andrea Manjarrez Trejo |  A01770068 - Oliver Bautista Gómez | A01770448 - Macarena Madrid Mañón | A01368255

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Life Cycle Assessment of pilot scale production of seaweed-based bioplastic, Team 1

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Life Cycle Assessment of pilot scale production of seaweed-based bioplastic, Team 1

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José Pablo Iglesias Zendejas    | A01770071
Victor Manuel Villafaña Velarde | A01367651
Evelyn Angélica Zarza González| A01770315

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Bioplastic made from seaweed polysaccharides with green production methods 

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Bioplastic made from seaweed polysaccharides with green production methods 

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María José Veytia Muhlia | A01770069
Yulyet Alessandra Zavala Rangel | A01067988
Stephany Lanzagorta | A01367509
Ian Contreras A01769936

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https://view.genial.ly/646d8fe5838999001822a9ea/interactive-content-activity-51-new-technologies-for-biotechnological-processes

Team members:
María José Anton
Karina Orta
Shurabe Mercado
Camila Noria

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Camila Noria González A01368000

Shurabe Danae Mercado Guido A01367715

María José Antón Hernández A01367550

Karina de Jesús Orta Cruz A01770060

Conclusions

Environmental Impact

We conclude that are many disadvantages for the conventional methods regarding the environment and bulk production due the the use of different chemical agents and solvents. Also, the bioextraction method has been the better solution for PHB extraction because of its economic and evironmental benefits.

The study discusses various methods for extracting polyhydroxyalkanoate (PHA), a biopolymer that can be used as a sustainable alternative to traditional plastics. It highlights the environmental impact of conventional extraction methods, which often involve the use of chemicals and solvents that can be harmful to the environment. Bioextraction methods, which use enzymes and other eco-friendly solvents, are presented as a more sustainable alternative. The report also discusses the potential of bacteriophage-related systems, predatory systems, and mealworm digestive systems for PHA extraction, which could further reduce the environmental impact of the process. Overall, the authors emphasize the importance of developing targeted green technology approaches for PHA extraction that are both highly pure and eco-friendly.

This review discusses the increasing use of bioplastics, specifically PHB, and the drawbacks of traditional extraction methods. Harmful to the environment and economically costly, there is a rising interest in bioextraction methods as greener alternatives. These methods utilize genetic and metabolic engineering, as well as predatory bacteria and mealworms, to extract purified PHB in an eco-friendly system. By combining biotechnology with conventional approaches, environmental limitations and costs can be reduced, leading to commercially viable extraction methods. Overall, bioextraction methods offer a more environmentally friendly and sustainable approach to extracting PHB from bacterial producer cells.

Haddadi, M. H., Asadolahi, R., & Negahdari, B. (2019). The bioextraction of bioplastics with focus on polyhydroxybutyrate: A review. International journal of environmental science and technology, 16, 3935-3948.

The primary steps of the methodoly are harvesting cell pretreament, polymer extraction, and post-treatment purification. Acording to the literature, for the extraction and purification of PHB methods are the solvent extraction that involves dissolving PHB in different components by precipitation in methanol and ethanol. Then they use chemical extraction that involves the separate the cell wall and extract PHB with a purity range of 86% to 99%. The surfactant-chelate extraction used chelants agents to extract PHB and it has the advantaje of being cheap. Also, the enzymatic digestion was used for the extraction but it is an expensive method and last the bioextraction system has a potencial because of the bacteriophage-related, predatory and mealworm digestion systems. The focus is on developing targeted green technology approaches for PHA extraction that are both highly pure and eco-friendly.

Main focus of the methodology

Innovation associated with the technology for bioplastics production

Mealworm digestive
What are bioextraction methods and what do they aim to address?
Bioextraction methods aim to address the concerns of environmental pollution and cost by utilizing green tools such as genetic and metabolic engineering of PHB-accumulating cells. These methods involve self-disruption, induction, and modification of predatory bacteria, as well as the utilization of the mealworm digestive system. By leveraging biotechnology mechanisms, manufacturers can extract purified PHB biopolymer in an eco-friendly system, using water and acetic acid for pre-treatment instead of more harmful reagents like SDS and chloroform.
Why is cell engineering a key aspect of bioextraction?
Cell engineering is a key aspect of bioextraction, but it is accompanied by modifications to culture conditions and feedstocks to overcome the limitations and drawbacks of conventional approaches. While these challenges are yet to be fully solved, the use of biotechnological approaches in bioextraction methods can help reduce their impact. By integrating biological techniques with conventional approaches, it becomes possible to overcome environmental limitations and reduce costs through genetically modified producers and optimized culture conditions. This paves the way for the establishment of suitable and commercially viable PHB extraction methods.

There are several innovative approaches associated with the technology for bioplastics production. These include: 1. Using wastewater sludge as a raw material for biopolymer production 2. Using enzymes in the extraction of polyhydroxyalkanoates (PHAs) produced by Cupriavidus necator 3. Using bacteriophages to prevent Pseudomonas aeruginosa contamination and fouling in Jet A aviation fuel 4. Using predatory bacteria and mealworms for bulk PHB extraction 5. Developing bio-based extraction methods through genetic and metabolic engineering and culture modification to produce highly pure PHB biopolymers and decrease additional chemical and solvents 6. Using landfill methane recycling for sustainable bioplastic production 7. Producing PHAs from fermented cheese whey using a mixed microbial culture 8. Developing biologically recovered PHAs through selective digestion of bacterial biomass in animals.
Lysis system
Starts with different systems like holin, endolysin, late transcriptional, and tandem. The holin-endolysin system acts as creel to endolysin through the inner membrane, at the same time the cytoplasm is suspended.
Predatory system
Carbon and energy sources become for living microorganisms for the metabolism and surrounding production happened in the intracelular and extracelular degradation of PHB.
Mealworm digestive
Many organisms contain copolymers, first shown in the PHB recovery when rats were feed on lyophilized C. Mercator H16

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Activity 5.1 - New Technologies for biotechnological processes

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Camila Noria González A01368000 Shurabe Danae Mercado Guido A01367715 María José Antón Hernández A01367550 Karina de Jesús Orta Cruz A01770060 Conclusions Environmental Impact We conclude that are many disadvantages for the conventional methods regarding the environment and bulk production due the the use of different chemical agents and solvents.