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Gelatin foam cell blocks made from cytology fluid specimens

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E.g. cells are collected by scraping, swabs, brushing or washing from  tissue surfaces.

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E.g. Serous fluids, CSF

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Naturally passed and freely available for collection 

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Naturally passed and freely available for collection 

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E.g. sputum, urine

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- SurePath or ThinPrep

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- AIR-DRIED (In the lab, these air-dried smears are rehydrated by placing in normal saline for 30 seconds, and then they are fixed in 95% alcohol, and then stained with Romanowsky Stains like MGG, Leishman or DiffQuik, or other special stains)
- ALCOHOL-FIXED / WET FIXED smears (Smears immediately fixed with cytology spray fixative or immerse in 95% Ethyl Alcohol for at least 20 minutes. Stained with Pap stain)

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1. Quick aspiration (avoid blood clot)
2. Quick transfer of material onto the glass slides
3. Proper smearing (avoid crushing the cells)
4. Immediate fixation (avoid air drying of specimen )
5. Stain with cytological stain like Pap stain (For alcohol fixed slide)
6. Stain with Romanowsky Stain or DiffQuik (For air dried slide)
7. Cell Block preparation (optional step)

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Pathologist will examine the cells and determine if they are BENIGN or MALIGNANT 

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Pathologist will examine the cells and determine if they are BENIGN or MALIGNANT 

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Cells --> Transferred to a glass slide  --> visualized under a microscope 

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Cells --> Transferred to a glass slide  --> visualized under a microscope 

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Obtianed by           PHYSICAL / MECHANICAL EXFOLIATION  

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Obtianed by           PHYSICAL / MECHANICAL EXFOLIATION  

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Obtained by SPONTANEOUS EXFOLIATION into a body fluid --> Cells have been spontaneously shed by the body

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Obtained by SPONTANEOUS EXFOLIATION into a body fluid --> Cells have been spontaneously shed by the body