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Bibsonomy | Mahendra Kumar Trivedi | Biofield

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Bibsonomy | Mahendra Kumar Trivedi | Biofield

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View the biofield publications of Mahendra Kumar Trivedi on Bibsonomy. Mr. Mahendra Trivedi is a Founder of The Trivedi Effect. Follow him on Bibsonomy to get in touch.

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Mahendra Kumar Trivedi’s Researches in Materials Science

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Mahendra Kumar Trivedi’s Researches in Materials Science

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Mahendra Kumar Trivedi is a man with supernatural powers and to validate hi power he has performed many researches. Know the impact of biofield treatment on the spectral properties of paracetamol and piroxicam Trivedi Effect, Materials Science

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Effect of Biofield Treatment on Spectral Properties of Paracetamol and Piroxicam

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Paracetamol and piroxicam are non-steroidal anti-inflammatory drugs (NSAIDs), widely used in pain and inflammatory diseases. The present study aimed to evaluate the impact of biofield treatment on spectral properties of paracetamol and piroxicam. The

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Mahendra Kumar Trivedi in International Conference

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Mahendra Kumar Trivedi in International Conference

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Know the effect of Mahendra Trivedi’s thought intervention through biofield in his physical presence on the atomic, crystalline and particle characteristics of first series of transition metal powders, group four metals and carbon allotropes.

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Mahendra Kumar Trivedi’s Biofield Treatment

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Mahendra Kumar Trivedi’s Biofield Treatment

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Mahendra Kumar Trivedi’s Biofield Treatment has shown the miraculous results. The treatment has substantially altered the atomic and physical properties of treated magnesium powder.

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Mendeley Supports Responsible Sharing Learn how you can share

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Impact of Biofield Treatment On Physicochemical Properties

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Impact of Biofield Treatment On Physicochemical Properties

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Know the impact of biofield treatment on physicochemical properties of hydroxyethyl cellulose and hydroxypropyl cellulose. Click here for more!

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Influence of Biofield Treatment on Physicochemical Properties of Hydroxyethyl Cellulose and Hydroxypropyl Cellulose

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Cellulose based polymers have shown tremendous potential as drug delivery carrier for oral drug delivery system (DDS). Hydroxyethyl cellulose (HEC) and hydroxypropyl cellulose (HPC) are widely explored as excipients to improve the solubility of

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Mahendra Kumar Trivedi’s Biofield Treatment on Barium Titanate Powder

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Mahendra Kumar Trivedi’s Biofield Treatment on Barium Titanate Powder

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Mahendra Kumar Trivedi’s Biofield Treatment had enhanced the density of barium titanate upto 1.38% as compared to control. To know more visit Academia and read the detailed report.

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Impact of Biofield Treatment on Atomic and Structural Characteristics of Barium Titanate Powder

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Barium titanate, perovskite structure is known for its high dielectric constant and piezoelectric properties, which makes it interesting material for fabricating capacitors, transducer, actuator, and sensors. The perovskite crystal structure and

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Biofield Treatment - Changes in Pharmaceutical Compound

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Biofield Treatment - Changes in Pharmaceutical Compound

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Mahendra Kumar Trivedi’s biofield treatment has substantially changed the various physicochemical and structural properties of Metronidazole and Tinidazole. To know about the research click here.

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Atomic and Structural Characteristics of Manganese Oxide

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Atomic and Structural Characteristics of Manganese Oxide

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Present study investigates impact of Biofield treatment on physical and atomic properties of Mn3O4. Read complete research on Google Scholar.

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Biofield Treatment: An Effective Strategy – Read Here

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Biofield Treatment: An Effective Strategy – Read Here

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Biofield Treatment is an effective strategy that helps to improve the quality of beef extract and meat infusion powder. To learn more read this paper.

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Do you want to know the effect of Biofield Treatment on Multidrug-resistant Burkholderia cepacia? Read Mahendra Kumar Trivedi researches in the field of Microbilogy with the Biofield Treatment.

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Read about Biofield Treatment on Pseudomonas fluorescens
	

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Read about Biofield Treatment on Pseudomonas fluorescens
	

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The objective behind this study was to investigate the effect of biofield treatment on antimicrobial sensitivity pattern of P. fluorescens.

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Antimicrobial Sensitivity Pattern of Pseudomonas fluorescens after Biofield

1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal- 462026 Madhya Pradesh, India

*Corresponding author: Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal-

Copyright: © 2015 Trivedi. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use,

distribution, and reproduction in any medium, provided the original author and source are credited.

Global emergence of Pseudomonas fluorescens (P. fluorescens) displays a mechanism of resistance to all

existing antimicrobials. Due to its strong ability to acquire resistance, there is a need of some alternative treatment

strategy. Objective of this study was to investigate the effect of biofield treatment on antimicrobial sensitivity pattern

of P. fluorescens. P. fluorescens cells were procured from MicroBioLogics in sealed packs bearing the American

Type Culture Collection (ATCC 49838) number. Two sets of ATCC samples were taken in this experiment and

denoted as A and B. ATCC-A sample was revived and divided into two groups (Gr) i.e. Gr.I (control) and Gr.II

(revived); likewise, ATCC-B was labeled as Gr.III (lyophilized). Gr.II and III were given biofield treatment and were

measured by MicroScan Walk-Away® system before and after treatment. Parameters studied in experiment were

antimicrobial sensitivity, minimum inhibitory concentration (MIC), biochemical reactions, and biotype number of both

control and treatment groups using MicroScan Walk-Away® system. Experimental results showed antimicrobials

showed altered sensitivity and MIC values in treated group as compared to control. Biochemical reactions showed

positive reaction in malonate, melibiose, nitrate, galactosidase, ornithine, raffinose, sorbitol, sucrose, tobramycin and

Voges-Proskauer in Gr.II. Arabinose, colistin, glucose, and rhaminose also showed positive reactions in Gr.II on day

10 while arginine and cetrimide showed negative reaction in Gr.III as compared to control. Biochemical tests results

revealed a change in biotype number in Gr.II (34101173, day 5), (77103177, a very rare biotype on day 10) and

Gr.III (40000043) as compared to control (02041722). Organism was identified as Enterobacter cloacae (GrII, day

10) and Vibrio fluvialis (Gr.III, day 10) with respect to control. These findings suggest that biofield treatment made

significant alteration in sensitivity pattern, MIC values, and biotype number of P. fluorescens.

; Antibiotic susceptibility;

Biofield treatment; Biochemical reactions; Biotyping

obligate aerobic, and considered as a psychrotrophic microorganism,

unable to grow at temperatures above 32°C. Fluorescent species of

pseudomonas, initially considered to be nonpathogenic for human.

Since 1953, human pathogenicity has been reported, when Pittman

describes its presence and characteristics in blood and caused severe to

opportunistic pathogen, and able to inhabit in many environment like

P.

have been reported for high hemolytic activity, which

also resides in the plant's rhizosphere

and produces a variety of secondary metabolites including antibiotics

P.

causing nosocomial infections even higher incidence rate

[3]. Some of these strains found in human digestive

tract with low commensal level while some found in refrigerated food

products with its psychrotrophic characters [4]. Recently, a specific

antigen of was detected in serum of ileal Crohn's disease patient [5].

P.

considered as a pathogenic microorganism. Different antimicrobial

combination therapies are preferred by microbiologist to alter its

sensitivity but due to their associated side effects, alternate approaches

are adopted by healthcare professionals. Among various alternate and

complementary therapies, biofield might be a new approach to do

permeates and surrounds living organisms. It is the scientifically

preferred term for the biologically produced electromagnetic and

functions within the organism. In spite of countless study reports of

the effectiveness of biofield therapies [6], there are very few well

controlled and peer-reviewed experimental studies [7]. According to

law of mass-energy inter-conversion [8], the conversion of mass into

energy is well stabilized, but its inversion i.e. energy into mass is not

yet proved scientifically. Whenever these electrical signals fluctuate

with time, the magnetic field generates as per the Ampere-Maxwell

law, and cumulatively known as electromagnetic field. As responses by

humans can be accounted for by the placebo effect, these experiments

on lower organisms are designed in order to directly test the impact of

biofield energy through scientific studies to rule out the placebo effect.

It is widely accepted that lyophilization is the most preferred method

to store and transport microbial cultures. Therefore, changes in the

biochemical and enzymatic characteristics of an organism cannot be

possible at this storage condition. Mr. Mahendra Trivedi's biofield

Infectious Diseases and TherapyJana et al., J Infect Dis Ther 2015, 3:3

http://dx.doi.org/10.4172/2332-0877.1000222

ISSN:2332-0877 JIDT, an open access journalVolume 3 * Issue 3 * 1000222

treatment has been studied significantly and its effects are considerably

microbes against tested antimicrobials [9-11]. However, researchers

found significant inhibitory effect of biofield in different cancer cell

models [12-14]. It has also shown significantly altered the crystalline

and powder characteristics of metals [15,16]. In agriculture, biofield

treated crops have been reported for a significant change on growth,

characteristics and yield of plants [17-19].

There are scanty reports on investigating biofield therapies against

microbes. In the present study, impact of biofield treatment on

P.

was evaluated and its antimicrobial susceptibility pattern,

MIC value, biochemical reactions, and biotype were studied in revived

as compared to control.

ATCC 49838 (American Type Culture Collection)

strains were procured from MicroBioLogics, Inc., USA, in two sets A

and B. The antimicrobials and biochemical used in the study were

procured from Sigma-Aldrich. Two different sealed packs were stored

with proper storage conditions until further use. The antimicrobial

estimated with the help of Microscan Walk-Away system® (Dade

Behring Siemens) using NBPC30 panel with respect to control groups.

Two ATCC samples (ATCC A and B) of

grouped and subjected to biofield treatment. ATCC A sample was

revived and divided into two groups (Gr) viz. Gr.I (control) and Gr.II

(revived); likewise, ATCC B was labeled as Gr.III (lyophilized). Then

group II and III were treated with Mr. Trivedi's biofield energy. Gr.II

was assessed on the 5th and 10th days after treatment while Gr.III was

assessed on 10th day only. Finally, all the groups (control and treated)

reactions pattern and biotyping.

Antimicrobial susceptibility assay

Antimicrobial susceptibility pattern of

using MicroScan Walk-Away® using NBPC30 panel as per

susceptibility pattern (S: Susceptible, I: Intermediate, and R: Resistant)

and minimum inhibitory concentration (MIC) were determined by

observing the change in antimicrobial concentration as per Clinical

and Laboratory Standards Institute (CLSI) guidelines [20].

Biochemical reaction and biotype number studies

organism identification after biofield treatment of

biochemical reactions patterns after biofield treatment were analyzed

using following 33 biochemical such as acetamide, adonitol, arabinose,

arginine, cetrimide, cephalothin, citrate, colistin, esculin, hydrolysis,

nitrofurantoin, glucose, hydrogen sulfide, indole, inositol, kanamycin,

sorbitol, sucrose, tartarate, tryptophan deaminase, tobramycin, urea,

Antimicrobial susceptibility assay

The data pertaining to the antimicrobial susceptibility tests and

details of MIC values were observed and reported in Table 1 and 2

respectively. The result of biofield treatment showed that sensitivity

pattern of ceftriaxone and tetracycline converted from I → R Gr.II on

day 5 and 10. Ceftazidime, ciprofloxacin and piperacillin, converted

from S → R in Gr.II on day 5 and 10 with changes in MIC values as

compared to control. Cefotaxime sensitivity pattern changed from I →

R in Gr.II while in cefepime, changed from I → R only at day 10 in

Gr.II. Tobramycin sensitivity pattern after treatment converted from S

→ I with altered MIC value on day 5 while S → R on day 10 in Gr.II. In

case of norfloxacin, change in MIC value was reported greater than 8

µg/mL in Gr.II as compared with control. Rest of tested antimicrobials

did not showed any change in susceptibility pattern and MIC values

after biofield treatment as compared to control (Table 2).

2Amoxicillin/K-clavulanate- - - -

3Ampicillin/Sulbactam - - - -

16Piperacillin/TazobactamS S S S

19Ticarcillin/K-ClavulanateR R R R

Citation: Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) Antimicrobial Sensitivity Pattern of Pseudomonas fluorescens after Biofield

Treatment. J Infect Dis Ther 3: 222. doi:10.4172/2332-0877.1000222

ISSN:2332-0877 JIDT, an open access journalVolume 3 * Issue 3 * 1000222

R: Resistant; I: Intermediate; S: Susceptible; '-': Not reported; Gr: group

Table 1: Effect of biofield treatment on antimicrobial susceptibility of

S.

2Amoxicillin/K-clavulanate>16/8 >16/8 >16/8 >16/8

3Ampicillin/Sulbactam>16/8 >16/8 >16/8 >16/8

5Aztreonam>16 >16 >16 >16

6Cefazolin>16 >16 >16 >16

10Cefoxitin>16 >16 >16 >16

14Cephalothin>16 >16 >16 >16

15Chloramphenicol>16 >16 >16 >16

16Ciprofloxacin≤1 >2 >2 ≤1

23Nitrofurantoin>64 >64 >64 >64

28 Ticarcillin/K-Clavulanate>64>64 >64 >64

Sulfamethoxazole >2/38 >2/38 >2/38 >2/38

MIC values are presented in µg/mL; Gr: Group

Table 2: Minimum inhibitory concentration (MIC) of

for tested antimicrobials

Biochemical tests denoted with codes are summarized in Table 3.

Results showed positive reactions (i.e. (-) negative to (+) positive) in

case of malonate, melibiose, nitrate, galactosidase, ornithine, raffinose,

sorbitol, sucrose, tobramycin, and Voges-Proskauer on day 5 and 10 in

Gr II on both days with respect to control. Arabinose, colistin, and

rhaminose showed negative reactions (i.e. (+) positive to (-) negative)

converted into positive to negative reaction in Gr.III and in Gr.II at

day 5 only. Cetrimide showed negative reaction in Gr.III as compared

to control. Glucose showed positive reaction at day 10 in Gr.II and

Gr.III as compared to control.

S.

Citation: Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) Antimicrobial Sensitivity Pattern of Pseudomonas fluorescens after Biofield

Treatment. J Infect Dis Ther 3: 222. doi:10.4172/2332-0877.1000222

ISSN:2332-0877 JIDT, an open access journalVolume 3 * Issue 3 * 1000222

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Antimicrobial Sensitivity Pattern of Pseudomonas fluorescens after Biofield Treatment - PdfSR.com

# data[].attributes.attachment_link.description
Global emergence of Pseudomonas fluorescens (P. fluorescens) displays a mechanism of resistance to all existing antimicrobials. Due to its strong ability to acquire resistance, there is a need of some alternative treatment strategy. Objective of this study was to investigate the effect of biofield treatment on antimicrobial sensitivity pattern of P.