# data[].attributes.body
Direct comparison

# data[].attributes.body
Emulsion PCR
- conducted on a bead surface within tiny water bubbles floating on an oil solution. (oil-water-base)
- one primer attached to surface of the bead
- used 454, polonator and SOLiD
-polymerase (pyrosequencing)
Bridge PCR
- Flank with adapter(solid surface, flow cell base)
- Flat  surface coated with 2 types of primer corresponding to adapter
-Solexa
- polymerase(reverse terminator)

# data[].attributes.body
Emulsion PCR
-Fragments, with adaptors, are PCR amplified within a water drop in oil.

-One primer is aiached to the surface of a bead
-Used by 454, Polonator and SOLiD.

Bridge PCR

-DNA fragments are flanked with adaptors.
- A flat surface coated with two types of primers, corresponding to the adaptors on a solid substrate
-Amplifica7on proceeds in cycles, with one end of each bridge tethered to the surface
- Used by Solexa.

# data[].attributes.body
Emulsion PCR
- bead-based
- vesicle only has 1 template present in each compartment
- oil-aqueous compartment
- primer is located on the surface of the bead
- only 1 adapter sequences is attached to the bead
- nucleotides not labelled
- no termination step
- asynchronous

Bridge PCR
- 'bridge' amplification reaction
- both known adapter sequences are attached to the solid substrate (chip)
- labelled nucleotides
- there is a washing, termination step
- synchronous

# data[].attributes.body
Emulsion PCR
fragments, with adapters, are pcr amplified within a water drop in oil. One primer is attached to the surface of a bead. Used by 454, Polonator and SOLiD.

Bridge PCR
DNA fragments are flanked with adaptors.
•  A flat surface coated with two types of primers, corresponding
to the adaptors.
•  Amplification proceeds in cycles, with one end of each bridge
tethered to the surface.
•  Used by Solexaillumina’s HiSeq platform

# data[].attributes.headline
2. Compare between emulsion PCR and Bridge PCR

# data[].attributes.subject
2. Compare between emulsion PCR and Bridge PCR

# data[].attributes.body
EMULSION PCR:
Emulsion PCR uses DNA products with attached adapters combined with beads that have one of the PCR primers to its surface while for Bridge PCR, the DNA products attached with adaptors placed on a chip, densely coated with adaptor primers to a solid surface

In Emulsion PCR, the PCR amplification takes place in a water-in-oil emulsion vesicle with only one template present in each compartment Bridge PCR, amplicons from a given template will remain tethered close to the point of origin

In Emulsion PCR, the amplicons are captured on the surface of the bead while in Bridge PCR,