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Lab modules are completed via the LMS to obtain appropriate certifications before placement.

Lab induction and tour of Heras Lab conducted by Begona Heras herself.

Khusnas mock presentation for her masters project.

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Preparing samples for proteins crystallization for Taylor's PhD project.

Inflammasome academic seminar talk.

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Attending the masters student presentations from different sectors of LIMS.

Attended the first lab meeting. where I got to introduce myself and the learn more about everyone's role at Heras Lab.

Feeding the galleria.

Obtaining and reading literature provided by Steph regarding DSB proteins.

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Assisting Khusna and Jason when they were culturing urinary bladder cells, monocytes, and hep2 cells.

Reading the literature provided by Khusna and Sushma regarding autotransporters.

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Observing and questioning Jason and Khusna as they stained the prepared cells lines and added antibodies for immunolabeling in order to visualize the internalization of pet-anti DDX3X into different cell lines using confocal microscopy.

Continued reading of literature provided by Khusna and Sushma regarding autotransporters.

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Observing and questioning Jason and Khusna as they stained the prepared cells lines and added antibodies for immunolabeling in order to visualize the internalization of pet-anti DDX3X into different cell lines using confocal microscopy.

Continued reading of literature

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Analyzing crystal growth in samples prepared with Taylor previously using microscopy.

Reporting crystal growth in different samples to Taylor.

Further reading of literature provided by Steph.

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Helping Khusna purify her initial PCR product using a commercial purification kit to help identify the issue behind the contamination of her construct.

Further reading of autotransporter literature provided by Khusna and Sushma.

Helping navigate and observe the use of Confocal microscopy on Khusna's previously prepared cell samples in order to evaluate the internalization of anit-pet DDX3X.

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Helping Khusna prepare new cell lines including breast, pancreas, and colorectal cell samples.

I was reading about Khusna's protocols for cell culturing and the PCR purification kit from the previous session in the lab with her.

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Confocal microscopy session to visualize the internalization of anti-pet DDX3X with the new cell lines. However this was cut short due to a cover slip issues.

Going through Khusna's and Sushma's presentation slides and autotransporter literature.

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Helping Khusna prepare new clines with cover slips for confocal microscopy of new cancer cells.m

Helping and observing Taylor purify proteins using HPLC and also utilize the peristatic pump to aid the HPLC.

I also helped Taylor check the concentration of these purified proteins samples using the nanodrop spectrometer.

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Confocal microscopy session with Khusna and help her obtain the images of internalization of pet anti-DDX3X in the new cancer cell samples.

I was reading through more of Sushma's literature that she sent previously.

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Helping Taylor prepare more samples for protein crystallization.

Helped Taylor analyze the formation of crystals in previously prepared samples.

Continue reading Taylor's ExDsbA protocols for ideal protein crystallization.

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Second academic seminar with the biochemistry department.

Observing Sushma and helping Sushma complete the transformation of her construct.

Helping Khusna and Sushma collect ordered materials that arrived in the store room.

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Helping Sushma purify her proteins using the HPLC.

Helping Sushma check the concentration of her protein samples using the nanodrop spectrometer.

Staining purified protein samples and using gel electrophoresis technology to analyze the samples.

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Helping Sushma check the purity of another batch newly purified proteins using the nano drop spectrometer.

Attended my second lab meeting.

Helping and observing Sushma prepare more gels for electrophoresis.

Observing Sushma clean the chromatography machine.

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Helping Taylor prepare more samples for protein crystallization.

Observing and helping Taylor analyze more protein crystallizations in previously prepared protein samples.

Observing Taylor used the general fluorescence reader to examine the effectiveness of inhibitors binding to DSB proteins.

Understanding the functioning of the general fluorescence reader from Taylor and how to analyze the graphs generated by the general fluorescence reader.

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Helping Taylor refill the pipette tip.

Helping and observing Taylor run the general fluorescence reader for three different inhibitors in 30 minute intervals.

Talking to Taylor and understanding how research projects are conducted in honors, masters and a PhD.

Reading literature to gain further understanding of the general fluorescence reader.

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Learning more from Taylor and furnishing an understanding of how to evaluate the results from the general fluorescence readers and how it indicates the efficiency of binding between inhibitors and DSB proteins.

Continued talking to Taylor regarding master's projects and the potential experience and value it can have on my future career.

Finished reading through Steph's literature that she previously emailed regarding DSB proteins.

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Going through the results of general fluorescence reader of other inhibitors with Taylor.

Observed prepare more gels for the rest of the protein samples she was purifying using the HPLC.

Continued reading through Sushma's literature that she sent earlier at the start of the placement.

Helped Sushma measure the concentration of her purified proteins using the nanodrop spectrometer.

Presented my experience throughout the placement with Begona Heras my supervisor.