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HepG2 HMCR Mo melt Curve

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HepG2 LC3 Mo Melt curve

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HepG2 FASN Mo Melt Curve

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HepG2 ACLY Mo Melt Curve

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qPCR analysis: LC3

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qPCR analysis: HMGCR

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qPCR analysis: FASN

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qPCR analysis: ACLY

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19/05/17-22/05/17
HepG2 RNA isolation and quantification

23/05/17
Standardize RNA and make cDNA

24/05/17
qPCR:
FASN and HMGCR

25/05/17
qPCR:
LC3 and ACLY

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Hi Alisa, Please see attached the 3 repeats for CV and MTT. I am busy with the 4th repeat of both CV and MTT . Thanks
Regards

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24/04/17-26/04/17
First MTT and CV repeat

08/05/17-10/05/17
Second MTT and CV repeat

15/05/17-19/05/17
Third MTT and CV repeat

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Split the cells and plated in 2 flasks

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1 June 2017

Thank you for the update.

Can we please update a representative melt curve (select the controls) for each gene for the MDAs and then for the HepG2s as well?

Thank you.

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13/04/17 - Discussed MTT and CV first repeat results. Did repeat of MTT and CV assays using low range of eicosanol. Changed media in 2 x T75. Could not split cos cells were stressed.

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12/04/17 - Made up CCM with EMEM and thawed cells

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Paper to use for qPCR fold change calculation

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Paper to use for qPCR fold change calculation

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Dialogue Box - Let us be kind, professional & courteous

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Dialogue Box - Let us be kind, professional & courteous

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AP: Are there any concepts in our plan of action that were unclear?

AP: Mary-anne, Please attend the MSc Committee meeting with Dr Soma. Send her an email indicating that you will need to since I am on leave. I will advise if anything changes.

AP: Sandra, Prof AJ will attend your PhD and ethics meeting with you.