# data[].attributes.headline
	
	
		
			Biofield | Properties of Dihydroxybenzophenone organic compound
		
	

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			Biofield | Properties of Dihydroxybenzophenone organic compound
		
	

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Present study aim was to investigate the influence of biofield energy treatment on the physical, thermal and spectral properties of 2,4-Dihydroxybenzophenone .

# data[].attributes.headline
	
	
		
			Spectral Properties of Biofield Treated 2,4-Dihydroxybenzophenone 
		
	

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			Spectral Properties of Biofield Treated 2,4-Dihydroxybenzophenone 
		
	

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The objective of this study was to investigate the influence of biofield energy treatment on the physical, thermal and spectral properties of 2,4-Dihydroxybenzophenone .

# data[].attributes.headline
	
	
		
			Mahendra Trivedi Biofield Energy Treated Dihydroxybenzophenone
		
	

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			Mahendra Trivedi Biofield Energy Treated Dihydroxybenzophenone
		
	

# data[].attributes.body
Butylatedhydroxytoluene (BHT) and 4-methoxyphenol (4-MP) are phenol derivatives that are generally known for their antioxidant properties and depigmenting activities. The aim of this study was to evaluate the impact of biofield energy treatment on the isotopic abundance in BHT and 4-MP using gas chromatography-mass spectrometry (GC-MS). BHT and 4-MP samples were divided into two parts: control and treated. The control group remained untreated while the treated group was subjected to Mr. Trivedi’s biofield treatment. Control and treated samples were characterized using GC-MS. The GC-MS data revealed that the isotopic abundance ratio of 13C/12C or 2H/1H (PM+1)/PM and 18O/16O (PM+2)/PM increased significantly in treated BHT and 4-MP (where PM- primary molecule, PM+1- isotopic molecule either for 13C or 2H and PM+2 is the isotopic molecule for 18O). The isotopic abundance ratio of (PM+1)/PM in the treated BHT and 4-MP was increased up to 181.27% and 380.73% respectively as compared to their respective control. Moreover, the isotopic abundance ratio of (PM+2)/PM in the treated BHT and 4-MP increased up to 185.99% and 355.33% respectively. GC-MS data suggests that the biofield treatment significantly increased the isotopic abundance of 2H, 13C and 18O in the treated BHT and 4-MP as compared to the control.

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			Biofield | Impact on Spectroscopic Characterization of Triphenylmethane
		
	

# data[].attributes.subject
	
	
		
			Biofield | Impact on Spectroscopic Characterization of Triphenylmethane
		
	

# data[].attributes.body
The aim of this study was to investigate the impact of Biofield treatment on physical, thermal and spectroscopical characterization of biofield treated triphenylmethane.

# data[].attributes.attachment_link.title
Physical, Thermal and Spectroscopical Characterization of Biofield Treated Triphenylmethane: An Impact of Biofield Treatment | Mahendra Trivedi | Alice Branton | Dahryn Trivedi | Gopal Nayak | Snehasis Jana | Academic Room

# data[].attributes.attachment_link.description
Triphenylmethane is a synthetic dye used as antimicrobial agent and for the chemical visualization in thin layer chromatography of higher fatty acids, fatty alcohols, and aliphatic amines. The present study was an attempt to investigate the impact of biofield treatment on physical, thermal and spectroscopical charecteristics of triphenylmethane.

# data[].attributes.headline
	
	
		
			Biofield | Effect on Spectroscopic Characterization of Triphenylmethane
		
	

# data[].attributes.subject
	
	
		
			Biofield | Effect on Spectroscopic Characterization of Triphenylmethane
		
	

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The present study was an attempt to investigate the impact of biofield treatment on physical, thermal and spectroscopical charecteristics of triphenylmethane.

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Trivedi et al., J Chromatogr Sep Tech 2015, 6:6

http://dx.doi.org/10.4172/2157-7064.1000292

Research ArticleOpen Access

Volume 6 * Issue 6 * 1000292

ISSN: 2157-7064 JCGST, an open access journal

Physical, Thermal and Spectroscopical Characterization of Biofield

Treated Triphenylmethane: An Impact of Biofield Treatment

Trivedi MK1 , Branton A1 , Trivedi D1, Nayak G1, Bairwa K2 and Jana S2*

1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal, Madhya Pradesh, India

*Corresponding author:Snehasis Jana, Trivedi Science Research Laboratory

Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd.,

Bhopal-462 026, Madhya Pradesh, India, Tel: +91-755-6660006; Fax: +91-755-

Citation:Trivedi MK, Branton A, Trivedi D, Nayak G, Bairwa K, et al. (2015)Physical,

Thermal and Spectroscopical Characterization of Bioeld Treated Triphenylmethane:

An Impact of Bioeld Treatment. Method Validation and Estimation of the Uncertainty.

Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed

under the terms of the Creative Commons Attribution License, which permits

unrestricted use, distribution, and reproduction in any medium, provided the

original author and source are credited.

Triphenylmethane is a synthetic dye used as antimicrobial agent and for the chemical visualization in

thin layer chromatography of higher fatty acids, fatty alcohols, and aliphatic amines. The present study was an

attempt to investigate the impact of bioeld treatment on physical, thermal and spectroscopical charecteristics of

triphenylmethane. The study was performed in two groups i.e., control and treatment. The treatment group subjected

to Mr. Trivedi's bioeld treatment. The control and treated groups of triphenylmethane samples were characterized

using X-ray diffraction (XRD), surface area analyzer, differential scanning calorimetry (DSC), thermogravimetric

mass spectrometry (GC-MS). XRD study revealed decreases in average crystallite size (14.22%) of treated

triphenylmethane as compared to control sample. Surface area analysis showed a slight increase (0.42%) in surface

area of treated sample with respect to control. DSC thermogram of treated triphenylmethane showed the slight

increase in melting point and latent heat of fusion with respect to control. TGA analysis of control triphenylmethane

showed weight loss by 45.99% and treated sample showed weight loss by 64.40%. The Tmax was also decreased

by 7.17% in treated sample as compared to control. The FT-IR and UV spectroscopic result showed the similar

pattern of spectra. The GC-MS analysis suggested a signicant decrease in carbon isotopic abundance (expressed

in δ13 C, ‰) in treated sample (about 380 to 524‰) as compared to control. Based on these results, it is found that

bioeld treatment has the impact on physical, thermal and carbon isotopic abundance of treated triphenylmethane

with respect to control.

Keywords: Triphenylmethane; Bioeld treatment; X-ray diraction;

Dierential scanning calorimetry; ermogravimetric analysis; Gas

chromatography-Mass Spectrometry (GC-MS)

XRD: X-ray diraction; DSC: Dierential scanning calorimetry;

TGA: ermogravimetric analysis; DTA: Dierential thermal analysis;

FT-IR: Fourier transform infrared; UV-Vis: Ultraviolet-visible; GC-

MS: Gas chromatography-mass spectrometry; PM: Primary molecule

Triphenylmethane is a hydrocarbon with molecular formula

as bromocresol green, malachite green etc., and used as pH indicator

and uorescence agent [1]. Boulos RA has reported its antimicrobial

property [2]. Triphenylmethane reported to inhibit 3-methyl-

cholanthrene-induced neoplastic transformation of 10T1/2 cells in a

dose-dependent manner and as a novel chemo preventive agent [3].

is has been used as visualizing agentin thin-layer chromatography

of higher chain fatty acids, fatty alcohols, and aliphatic amines

[4]. Recently, triphenylmethane was reported as an alternative for

mediated electronic transfer systems in glucose oxidase biofuel cells

(enzymatic biofuel cell). e enzymatic biofuel cell is a type of fuel cell

wherein the enzymes are used as a catalyst to oxidize its fuel, instead

of costly metals [5]. Hugle et al. used triphenylmethane as a possible

moderator material to reduces the speed of neutrons in nuclear chain

reactions, especially promising as cold neutrons moderator. It has

a unique structure i.e., three aromatic phenyl groups surrounding

one central carbon atom that is able to generate a stable radical ion

[6]. Diverse applications of triphenylmethane especially as orescent

indicator, mediator in biofuel and as a moderator had been suggested

the importance of physicochemical property of triphenylmethane.

It was previously reported that physical and thermal properties of

molecule also aect its reactivity [7,8]. Hence, it is benecial to nd

out an alternate approach that can improved the physicochemical

properties of compounds like triphenylmethane, which can enhance

its usability. Recently, bioeld treatment reported to alter the spectral

properties of various pharmaceutical drugs like chloramphenicol and

tetracycline, and physicochemical properties of metals, beef extract and

meat infusion powder [9-11].

Relation between mass and energy (E=mc2 ) is well reported

in literature [12]. The mass (solid matter) is consist of energy

and when this energy vibrates at a certain frequency, it provides

physical, atomic and structural properties like size, shape, texture,

crystal structure, and atomic weight to the matter [13]. Similarly,

human body also consists with vibratory energy particles like

protons, neutrons, and electrons [14]. Due to vibrations in these

particles, an electrical impulse is generated that cumulatively

forms electromagnetic field, which is known as biofield [15]. The

human has the ability to harness the energy from the environment

or Universe and transmit this energy into any object (living or

nonliving) on the Globe. The object(s) receive the energy and

respond into useful way, this process is known as biofield treatment.

Citation: Trivedi MK, Branton A, Trivedi D, Nayak G, Bairwa K, et al. (2015)Physical, Thermal and Spectroscopical Characterization of Bioeld Treated

doi:10.4172/2157-7064.1000292

Volume 6 * Issue 6 * 1000292

ISSN: 2157-7064 JCGST, an open access journal

ermogravimetric analysis-dierential thermal analysis

ermal stability of control and treated triphenylmethane were

analyzed using Mettler Toledo simultaneous TGA and dierential

thermal analyzer (DTA). e samples were heated from room

temperature to 400°C with a heating rate of 5°C/min under air

atmosphere. Percent change intemperature at which maximum weight

loss occurs in sample was calculated.

For determination of FT-IR and UV-Vis spectroscopic characters,

the treated sample was divided into two groups i.e., T1 and T2. Both

treated groups were analyzed for their spectral characteristics using FT-

IR and UV-Vis spectroscopy as compared to control triphenylmethane

sample. While, for GC-MS analysis, the treated sample was divided into

four groups i.e.,T1, T2, T3, and T4 and all treated groups were analyzed

along with control sample for isotopic abundance of carbon-13.

FT-IR spectroscopic characterization

FT-IR spectra of control and treated samples of triphenylmethane

were recorded on Shimadzu's Fourier transform infrared spectrometer

(Japan) with frequency range of 4000-500 cm-1. e analysis was

accomplished to evaluate the eect of bioeld treatment at atomic level

like dipole moment, force constant and bond strength in chemical

UV-Vis spectroscopic analysis

UV spectra of control and treated samples of triphenylmethane

were obtained from Shimadzu UV-2400 PC series spectrophotometer.

A quartz cell with 1 cm and a slit width of 2.0 nm was used for analysis.

e study was carried out using wavelength in the range of 200-400

nm. e UV spectra were analyzed to determine the eect of bioeld

treatment on the energy gap of bonding and nonbonding transition of

Gas chromatography-mass spectroscopy (GC-MS) analysis

e GC-MS analysis of control and treatment samples (T1, T2, T3, and

T4) of triphenylmethane were performed on Perkin Elmer/auto system XL

with Turbo mass and electron ionization mode, USA. Detection limit was

set to 1 Pico gram, and mass range was set to 10-650 amu. e isotopic ratio

13C/12C was expressed by its deviation in treated triphenylmethane sample

with respect to control. e isotopic abundance of 13C was computed on

a delta scale per thousand. e values of δ13 C of treated samples were

calculated using following equation [27].

m/z=244 for δ13C in mass spectra of treated and control samples

respectively.

XRD of control and treated triphenylmethane are presented in

Figure 1. e control triphenylmethane showed the XRD peaks at 2θ

equals to 11.70°, 11.91°, 15.00°, 18.24°, 19.67°, 22.52°, 22.66°, 23.99°,

24.96°, 26.19°, and 28.71°. However, the XRD diractogram of treated

triphenylmethane showed the decrease in intensity of the peaks. XRD

peaks in treated sample were appeared at 2θ equals to 11.92°, 12.33°,

14.87°, 15.03°, 18.25°, 19.66°, 20.76°, 22.52°, 23.99°, 24.24°, and 26.19°.

Mr. Trivedi's unique biofield energy is also called as The Trivedi

Effect® , and reported to change various physicochemical, thermal

and structural properties of several metals [10,16,17] and ceramics

[18]. In addition, biofield treatment has been extensively studied in

different fields such as agricultural science [19,20], biotechnology

research [21], and microbiology research [22-24].

Conceiving the impact of bioeld treatment on various living

and nonliving things, the study aimed to evaluate the impact of

bioeld treatment on spectral and physicochemical properties of

triphenylmethane using dierent analytical techniques.

Triphenylmethane was procured from Sisco Research Laboratories,

India. e study was performed in two groups i.e., control and

treatment. e control sample was remained as untreated; and

treatment sample was handed over in sealed pack to Mr. Trivedi for

bioeld treatment under laboratory conditions. Mr. Trivedi provided

the bioeld treatment through his energy transmission process to the

treatment group without touching the sample [9]. e control and

treated samples of triphenylmethane were evaluated using various

analytical techniques like X-ray diraction (XRD), surface area

analyzer, dierential scanning calorimetry (DSC), thermogravimetric

analysis (TGA), Fourier transform infrared (FT-IR), ultraviolet-visible

(UV-Vis) spectroscopy, and gas chromatography-mass spectrometry

X-ray diraction (XRD) study

XRD analysis of triphenylmethane was performed on Phillips,

Holland PW 1710 X-ray diractometer with copper anode and

nickel lter. Wavelength of X-ray used in XRD system was 1.54056 Å

with scanning rate of 0.05° 2/s, and a chart speed of 10 mm/2. Data

obtained from XRD system were in the form of a chart of 2θ (10-100°)

vs. intensity. e average crystallite size (G) of triphenylmethane was

calculated using the following equation [25].

powder samples respectively.

Surface area of control and treated triphenylmethane was measured

using the Brunauer-Emmett-Teller (BET) surface area analyzer,

Smart SORB 90. Percent changes in surface area were calculated using

%changeinsurfacearea100

Where, S Control and S Treated are the surface area of control and treated

Dierential scanning calorimetry (DSC) study

e control and treatment samples of triphenylmethane were

analyzed using a Pyris-6 Perkin Elmer dierential scanning calorimeter

(DSC) on a heating rate of 10°C/min under air atmosphere with air

ow rate of 5 mL/min. An empty pan sealed with covered aluminum

pan was used as a reference. e melting temperature (Tm ) and latent

heat of fusion (ΔH) were obtained from the DSC curve.

Citation: Trivedi MK, Branton A, Trivedi D, Nayak G, Bairwa K, et al. (2015)Physical, Thermal and Spectroscopical Characterization of Bioeld Treated

doi:10.4172/2157-7064.1000292

Volume 6 * Issue 6 * 1000292

ISSN: 2157-7064 JCGST, an open access journal

e sharp and intense peak in XRD diractogram of control and

treated samples suggested the crystalline nature of triphenylmethane

in both samples. e result showed that the XRD peaks were shied

aer bioeld treatment as 1191°→1192°; 1500°→15.03°; 18.24°→18.25°;

19.67°→19.66°; 24.96°→24.24° etc. moreover, few peaks like 11.70° and

28.7° in control sample are disappeared or their intensity is decreased

aer bioeld treatment. e decrease in intensity of XRD peaks in

bioeld treated triphenylmethane might be attributed to decrease in

long-range order of the molecules. e average crystallite size was

calculated using Scherrer formula and the result are shown in Figure 2.

e average crystallite size of control triphenylmethane was found as

117.17 nm that was decreased to 100.51 nm in treated sample. e result

showed about 14.22% decrease in average crystallite size in treated

sample as compared to control. It was reported that increase in internal

micro strain leads to decrease the corresponding crystallite size of the

material [27]. Moreover, Zhang et al. showed that presence of strain

and increase atomic displacement from their ideal lattice positions

causes reduction in crystallite size [28]. Hence, it is assumed that

bioeld treatment may induce the internal strain in triphenylmethane.

is might be the responsible for decrease in average crystallite size of

the treated triphenylmethane as compared to control.

e surface area of control and treated samples of triphenylmethane

was determined using BET surface area analyzer and data are presented

in Figure 3. e control sample showed a surface area of 0.8243 m2 /g;

however, the treated sample of triphenylmethane showed a surface

area of 0.8278 m2/g. e result showed a slight increase in surface

area (0.42%) in the treated triphenylmethane sample as compared to

control. e increase in surface area might be correlated to particle size

reduction due to high internal strain produced by bioeld treatment

[29]. e increase in surface area may lead to increase in solubility [30]

and reactivity of triphenylmethane as compared to control.

DSC was used to analyze the melting temperature and latent heat of

fusion (ΔH) of control and treated triphenylmethane. In solid materials,

substantial amount of interacting forces exist in atomic level that hold the

atoms at their positions. ΔH is dened as the energy needed to overcome

the interaction force to change the solid phase into liquid phase. Hence,

the energy provided during phase change i.e., ΔH is stored as potential

energy of atoms. However, melting point is related with kinetic energy

of the atoms [31]. DSC thermogram showed the melting temperature

at 94.57°C in control and 95.11°C in treated sample (Table 1), which

revealed about 0.57% increase in melting temperature in treated sample

of triphenylmethane with respect to control. e melting temperature of

triphenylmethane was well supported by literature data [32]. Likewise,

the DSC thermogram exhibited the latent heat of fusion i.e., 85.05 J/g in

control and 85.27 J/g in treated sample of triphenylmethane. e result

depicted about 0.26% change in latent heat of fusion of treated sample as

ermogravimetric analysis (TGA)/derivative

thermogravimetry (DTG) analysis

e TGA and DTG analysis of control and treated samples

of triphenylmethane are shown in Table 1. TGA thermogram of

control triphenylmethane exhibited the onset temperature around

216.00°C that was end-set around 257.00°C with 45.99% weight loss.

However, the treated triphenylmethane started losing weight around

193.00°C and end-set around at 248.00°C with 64.40% weight loss

(Figure 4). e result showed decrease in onset temperature of treated

triphenylmethane by 10.65% as compared to control. Moreover,

DTG thermogram exhibited the maximum thermal decomposition

Figure 1: XRD diffractogram of triphenylmethane.

Figure 2: Average crystallite size of control and treated triphenylmethane.

Figure 3: Surface area of control and treated triphenylmethane.

# data[].attributes.attachment_link.title
Physical, Thermal and Spectroscopical Characterization of Biofield Treated Triphenylmethane - PdfSR.com

# data[].attributes.attachment_link.description
Triphenylmethane is a synthetic dye used as antimicrobial agent and for the chemical visualization in thin layer chromatography of higher fatty acids, fatty alcohols, and aliphatic amines. The present study was an attempt to investigate the impact of biofield treatment on physical, thermal and spectroscopical charecteristics of triphenylmethane.

# data[].attributes.headline
	
	
		
			Biofield Effect on Spectroscopical Characteristics of Triphenylmethane
		
	

# data[].attributes.subject
	
	
		
			Biofield Effect on Spectroscopical Characteristics of Triphenylmethane
		
	

# data[].attributes.body
An experimental study - To see the impact of Mahendra Kumar Trivedi biofield treatment on physical, thermal and spectroscopical charecteristics of triphenylmethane.

# data[].attributes.attachment_link.title
"Physical, Thermal and Spectroscopical Characterization of Biofield Treated Triphenylmethane: An Impact of Biofield Treatment" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Triphenylmethane is a synthetic dye used as antimicrobial agent and for the chemical visualization in thin layer chromatography of higher fatty acids, fatty alcohols, and aliphatic amines. The present study was an attempt to investigate the impact of biofield treatment on physical, thermal and spectroscopical charecteristics of triphenylmethane.

# data[].attributes.headline
	
	
		
			An Experimental Study on Biofield Treated Triphenylmethane
		
	

# data[].attributes.subject
	
	
		
			An Experimental Study on Biofield Treated Triphenylmethane
		
	

# data[].attributes.body
Study more about the physical, thermal and spectroscopical characterization of Mr. Trivedi biofield treated Triphenylmethane.

# data[].attributes.headline
	
	
		
			Biofield Treatment | Spectroscopical Characteristics of Triphenylmethane
		
	

# data[].attributes.subject
	
	
		
			Biofield Treatment | Spectroscopical Characteristics of Triphenylmethane
		
	

# data[].attributes.body
To evaluate the impact of biofield treatment on physical, thermal and spectroscopical charecteristics of triphenylmethane, Mahendra Kumar Trivedi done this study.

# data[].attributes.headline
	
	
		
			Biofield | Spectroscopical Characterization of Triphenylmethane
		
	

# data[].attributes.subject
	
	
		
			Biofield | Spectroscopical Characterization of Triphenylmethane
		
	

# data[].attributes.body
Present study was designed to see the impact of Mahendra Kumar Trivedi biofield treatment on physical, thermal and spectroscopical charecteristics of triphenylmethane.

# data[].attributes.headline
	
	
		
			Mahendra Kumar Trivedi Biofield Impact on Triphenylmethane
		
	

# data[].attributes.subject
	
	
		
			Mahendra Kumar Trivedi Biofield Impact on Triphenylmethane
		
	

# data[].attributes.body
A test conducted to check the impact of Biofield treatment on physical, thermal and spectroscopical characterization of biofield treated Triphenylmethane.

# data[].attributes.attachment_link.title
Physical, Thermal and Spectroscopical Characterization of Biofield Treated Triphenylmethane: An Impact of Biofield Treatment

# data[].attributes.attachment_link.description
Triphenylmethane is a synthetic dye used as antimicrobial agent and for the chemical visualization in thin layer chromatography of higher fatty acids, fatty alcohols, and aliphatic amines. The present study was an attempt to investigate the impact of

# data[].attributes.headline
	
	
		
			Spectroscopical Characterization of Biofield Treated Triphenylmethane
		
	

# data[].attributes.subject
	
	
		
			Spectroscopical Characterization of Biofield Treated Triphenylmethane
		
	

# data[].attributes.body
Triphenylmethane is a synthetic dye used as antimicrobial agent. The present study was an attempt to investigate the impact of biofield treatment on physical, thermal and spectroscopical charecteristics of triphenylmethane.

# data[].attributes.headline
	
	
		
			Biofield | Spectral Properties of Triphenylmethane – Aftermath
		
	

# data[].attributes.subject
	
	
		
			Biofield | Spectral Properties of Triphenylmethane – Aftermath
		
	

# data[].attributes.body
The study to check the physical, thermal and spectroscopical characterization of biofield treated triphenylmethane after an impact of Biofield Treatment.