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Can oxaloacetate cross the mitochondria membrane?

Is NADPH created when transporting oxaloacetate?

Is NADH needed on the cytoplasm side?

What processing is needed on the mitochondrion side to get oxaloacetate? Does it require an enzyme?

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Oxaloacetate must be interconverted to return to the matrix.

1: CITRATE turns to OXALOACETATE and Acetyl CoA in the cytoplasm.

2: Oxaloacetate and NADH bind to form MALATE (still in cytoplasm)

3: Malate turn to pyruvate and NADPH (still un cytoplasm)

4: FInally, pyruvate CROSSES the membrane back into the mitochondrion (in mitochondrion).

5:In the mitochondria, the pyrivate is made BACK TO OXALOACETATE by the enzyme PYRUVATE CARBOXYLASE.

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Outline the mechanisms for the return of oxaloacetate to the mitocondria after citrate cleavage.

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To complete the shuttle, oxaloacetate must be transported from the matrix to the cytoplasm but there is no such transport system. Instead, oxaloacetate is first converted to aspartate by aspartate aminotransferase and then transported out of the mitochondria via the glutamate-aspartate translocase.