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"Filtering water samples for eDNA analysis" by ChicagoUSACE is licensed under CC BY 2.0
"Ghost" by Jordi Carrasco is licensed under CC BY 2.0
"Little boy responds to Adele's Hello" by VineMonster is licensed under Standard YouTube License
"Scientists move to make eDNA the next big thing for Oregon stream" by EarthFix is licensed under CC BY 2.0
"Working with environmental DNA (eDNA)" by USDA Forest Service is licensed under Standard YouTube License

Baldigo, B., Sporn, L.A., George, S., & Ball, J. (2017). Efficacy of environmental DNA to detect and quantify brook trout populations in headwater streams of the Adirondack Mountains, New York. Transactions of the American Fisheries Society, 146(1), 99-111.

Bista, I., Carvalho, G. R., Walsh, K., Seymour, M., Hajibabaei, M., Lallias, D., &Creer, S. (2017). Annual time-series analysis of aqueous eDNA reveals ecologically relevant dynamics of lake ecosystem biodiversity. Nature Communications, 8, 14087. http://doi.org.proxy.lib.miamioh.edu/10.1038/ncomms14087

Bylemans, J., Furlan, E., Gleeson, D., Pearce, L., & Daly, T. (2016). Improving the containment of a freshwater invader using environmental DNA (eDNA) based monitoring. Biological Invasions, 18(10), 3081-3089. doi:10.1007/s10530-016-1203-5

Ma, H., Stewart, K., Zhao, J., Lougheed, S., Wang, Y., & Zheng, J. (2016). Characterization, optimization, and validation of environmental DNA (eDNA) markers to detect an endangered aquatic mammal. Conservation Genetics Resources, 8(4), 561-568. doi:10.1007/s12686-016-0597-9

Roussel, J.-M., Paillisson, J.-M., Tréguier, A. and Petit, E. (2015). The downside of eDNA as a survey tool in water bodies. Journal of Applied Ecology, 52, 823–826. doi:10.1111/1365-2664.12428

U.S. Geological Survey. (2013). Application of environmental DNA for inventory and monitoring of aquatic species. Retrieved from https://pubs.usgs.gov/fs/2012/3146/pdf/fs2012-3146.pdf

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Sampling in aquatic environments allows for scientists to search for specific DNA markers, identifying certain species. It can also provide an overview of biodiversity in a body of water. Additionally, it does not in any way disturb the environment, so it can be a good option for fragile and protected spaces.

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As many of eDNA samples are taken in moving water, it is still unknown how far traceable DNA has traveled in the water (Roussel et al., 2015).

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A recent study compared the efficacy of eDNA in comparison with other sampling forms. It was discovered the DNA markers in water had a much longer persistence than eDNA in sediment (Baldigo et al., 2016).

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collecting samples (most commonly from aquatic environments) and analyzed for specific DNA markers which indicate the presence of certain species. Matt will give you an in-depth look at the extraction process

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Department of Environment , Water and Natural Resources

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Corps of Engineers Aquatic Nuisance Species

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Filtering Water Samples for eDNA Analysis

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U.S. Army Corps of Engineers (USACE) Fish Biologist Matthew Shanks explains the environmental DNA (eDNA) process at EPA labs, Chicago, July 2011. USACE works with other agencies to aggressively monitor the Chicago Sanitary and Ship Canal (CSSC) to determine the location and abundance of Asian carp in the area, using traditional and non-traditional methods, including eDNA analysis.

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eDNA is helping scientists study the biodiversity of aquatic environments over time (Bista et al., 2017). By sampling the same areas multiple times over the course of a year or longer, scientists can monitor trends in biodiversity.

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Scientists are using eDNA to help track populations of endangered species, like the Yangtze River dolphin (Ma et al., 2016).

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Listen to Kellie...she knows what's up.

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Department of Environment , Water and Natural Resources

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National Human Genome Research Institute

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Meet landscape ecologist Kellie Carim. Kellie is the eDNA Coordinator for the Rocky Mountain Research Station's National Genomic Center for Wildlife and Fish Conservation. In this video, Kellie demonstrates how it works and why this revolutionary process is a significant breakthrough in the monitoring of animal and fish species.

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Lack of DNA markers for certain species in a sample does not mean that species is not present in the environment. It simply is not present in that specific sample.

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Explore AirJOI's photos on Flickr. AirJOI has uploaded 288 photos to Flickr.

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This SoundCloud recording gives a good overview of how scientists in Oregon are using eDNA. Potentially, scientists can identify specific species and the abundance of those species from a small set of samples.

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Scientists Move To Make e-DNA The Next Big Thing For Oregon Streams by EarthFix

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Occasionally a big idea comes along that promises revolutionize the world - think about things like self-driving cars. For biologists - especially those who work with fish - the big idea involves something called "environmental DNA." The technology could revolutionize how we protect native animals and ensure invasive species don't take hold.

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eDNA samples can be used to determine the spread of invasive species and provide scientists with an idea of potential containment areas. (Byelmans, et al., 2016). Samples are taken from increasingly distant areas from the main population of invasives to determine the affected area.

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eDNA can be used to track secretive, rare, and small species

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Little boy responds to Adele's Hello Song

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DNA released from an organism into the environment (hair, saliva, mucus, skin, etc.)