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Gene editing in General - use, techniques, vectors

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plate bacteria step 2+: did not transfer sample to new tube

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plate bacteria step 2+: did not transfer sample to new tube

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How Defence systems work

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LacZ, blue/white screening, bacterial transformation, plasmid isolation, nanodrop

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PlzDonor and PLZDonorGuide

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LacZ gene

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Plasmids:
pLZDonor (control) vs pLZDonorGuide

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Plate pictures

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Explain CRISPR, Blue-white screening, plasmids (pLZDonor and pLZDonorGuide)

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Explain CRISPR, Blue-white screening, plasmids (pLZDonor and pLZDonorGuide)

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Colors of plated bacteria

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Nanodrop

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Why we got/didn’t get blue/white

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Discuss the growth and color of the bacteria om each plate

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Describe why the bacteria had the different colors

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Describe cellular growth on plates